LOCUS       sequence-199728-        8330 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Brownawell AM, Macara IG
  TITLE     Exportin-5, a novel karyopherin, mediates nuclear export of 
            double-stranded RNA binding proteins.
  JOURNAL   J Cell Biol. 2002 Jan 7. 156(1):53-64.
  PUBMED    11777942
REFERENCE   2  (bases 1 to 8330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2002 Jan 7. 156(1):53-64."
COMMENT     Sequence Label: pKmyc-Exp5
FEATURES             Location/Qualifiers
     source          1..8330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    82..103
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(118..123,124..141,142..148)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    156..172
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     161..183
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     180..196
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     180..196
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        225..604
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        605..808
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     759..779
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     805..829
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1039..1057
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1039..1056
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1070..1089
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             1144..1173
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(3706..3717)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      4841..4850
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4854..4988
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4891..4910)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4945..4964
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        5057..5414
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5057..5248
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5080..5100)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5265..5400
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5434..5451)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5434..5450)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5443..5465)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5663..6118
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5750..5769)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5960..5981
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6135..6154)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6254..6276
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6314..6332)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6400..6504
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6505..6573,6574..7365)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6723..6742)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7536..8124
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8025..8044
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8278..8295
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
       61 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
      121 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
      181 aggaaacagc tatgacatga ttacgaatta attcgagctc gcccgacatt gattattgac
      241 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      301 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      361 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      421 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      481 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      541 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      601 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      661 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      721 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      781 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      841 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccg
      901 ggaacggtgc attggaacgc ggattccccg tgccaagagt gacgtaagta ccgcctatag
      961 agtctatagg cccaccccct tggcttcgtt agaacgcggc tacaattaat acataacctt
     1021 atgtatcata cacatacgat ttaggtgaca ctatagaata acatccactt tgcctttctc
     1081 tccacaggtg tccactccca ggtccaactg cacctcggtt ctaagcttct gcaggtcgac
     1141 atggaacaga aactcatctc tgaagaggat ctgtctagag gatctatggc gatggatcaa
     1201 gtaaacgcgc tgtgcgagca gctggtgaaa gcggtgacgg tcatgatgga ccccaactcc
     1261 acccagcgct accggctgga agccctcaag ttttgtgagg agtttaaaga aaagtgtcct
     1321 atctgtgtcc cctgtggctt gaggttggct gagaaaacac aagttgccat cgtcagacat
     1381 tttggccttc agatcctgga acacgttgtc aagtttcggt ggaacggcat gtctcgattg
     1441 gagaaggtgt atctgaagaa cagtgtcatg gagctgattg caaatggaac attgaacatt
     1501 ttggaagagg agaaccatat taaagatgct ctgtctcgaa ttgtagtgga aatgatcaag
     1561 cgagagtggc cacagcattg gcctgacatg ctaatagaat tggacactct ttccaaacaa
     1621 ggggaaacac agacagaatt ggtgatgttt atccttttgc gactggcaga ggatgtagtg
     1681 acttttcaga cacttccccc tcaaagaaga agggacatcc agcaaacatt aacccagaac
     1741 atggaaagga tcttcagttt tctgcttaac acacttcaag aaaatgtaaa caagtatcag
     1801 caagtgaaga cagatacttc tcaggagtca aaggcgcaag caaactgtcg agtaggagtt
     1861 gcagcactga atactctagc aggctatatt gactgggtgt ctatgagtca catcactgct
     1921 gaaaactgta aactcctgga gatactgtgt ttgctgttga atgaacagga acttcagttg
     1981 ggagccgctg agtgtcttct cattgcagtc agcagaaaag gcaagttgga agaccggaag
     2041 cccttgatgg tcttatttgg agatgttgcc atgcattata tactctccgc cgcacagact
     2101 gctgatggag gaggtttggt agaaaaacac tacgtctttc tgaagaggct ctgtcaggtg
     2161 ttgtgtgcgc tgggcaatca gctgtgtgca ttgctgggtg cagattctga tgtagaaaca
     2221 ccatcaaact ttggaaaata cctggaatct tttcttgctt tcacaaccca tccaagtcag
     2281 tttctacgct cttcaactca gatgacttgg ggagccctct tcaggcatga aatcctgtcc
     2341 cgtgatcctt tgctattagc aataatacca aaatatcttc gtgcttccat gactaacttg
     2401 gtcaagatgg gctttccttc taaaacagac agccctagct gtgaatattc tcggtttgat
     2461 tttgatagcg atgaggactt caatgctttc ttcaactcct cccgagcaca acaaggagag
     2521 gtgatgaggt tggcatgtcg tttggatccc aaaactagct tccagatggc tggggagtgg
     2581 ctaaagtatc aactatcaac ttttcttgat gctggttctg tgaattcttg ttctgcagtt
     2641 ggaactggag aaggaagcct ctgttccgtc ttctcacctt cattcgtgca gtgggaagcc
     2701 atgactcttt ttttggaaag tgttatcacc cagatgtttc gaacactaaa tagagaagaa
     2761 attcctgtta atgatggaat agagctattg cagatggttc tgaactttga taccaaggat
     2821 cccctcatcc tgtcctgcgt ccttactaat gtctctgcac tctttccatt tgtcacctac
     2881 agaccagagt tcctgcccca ggtcttctct aagctatttt catctgtcac ttttgaaact
     2941 gttgaagaaa gtaaggcccc cagaacccgg gcagtgagga atgtgaggag gcatgcttgt
     3001 tcctccatca tcaagatgtg tcgtgactac ccccagcttg tgctgcccaa ttttgacatg
     3061 ctttataacc atgtgaagca actcctctcc aatgagctac tcctgacaca aatggagaag
     3121 tgtgccctca tggaagccct ggttctcatt agcaaccaat ttaagaacta cgagcgtcag
     3181 aaggtgttcc tagaggagct gatggcacca gtggccagca tctggctttc tcaagacatg
     3241 cacagagtgc tgtcagatgt tgatgctttc attgcgtatg tgggtacaga tcagaagagc
     3301 tgtgacccag gcctggagga tccgtgtggc ttaaaccgtg cacgaatgag cttttgtgta
     3361 tacagcattc tgggtgtggt gaaacgaact tgctggccca ctgacctaga agaggccaaa
     3421 gctgggggat ttgtggtggg ttatacatcc agtggaaatc caatcttccg taacccctgc
     3481 acagagcaga ttctgaaact tcttgacaat ttgcttgcgc ttataagaac ccacaataca
     3541 ttatatgcac cagaaatgct agccaaaatg gcagagcctt tcaccaaggc tctggatatg
     3601 cttgacgcgg aaaaatctgc tatattagga ttacctcaac ctctcttgga actcaatgac
     3661 tctcctgtct tcaaaaccgt cttggaaaga atgcagcgtt tcttctctac cctctatgaa
     3721 aactgttttc atatcctagg gaaggcaggc ccttccatgc agcaagactt ctacactgtg
     3781 gaggaccttg ctacccagct tctcagctca gcctttgtca acttgaacaa tattcctgac
     3841 taccgactca gacccatgct tcgtgtcttt gtaaagcctc tggtgctctt ctgtccccca
     3901 gagcactatg aagccctggt atcccccatc ctcggacctc ttttcaccta cctccatatg
     3961 aggctttctc agaaatggca agttatcaac caaaggagcc tgctgtgtgg agaagatgag
     4021 gctgcagatg aaaacccaga gtctcaagag atgctggagg agcaactggt gaggatgtta
     4081 acccgagaag tcatggacct aatcacggtt tgctgtgttt caaagaaggg tgctgaccac
     4141 agtagtgctc ccccagcaga tggagacgat gaagaaatga tggccacaga ggtcaccccc
     4201 tcagctatgg cagagcttac agacctgggc aaatgtctga tgaagcatga ggatgtttgt
     4261 acagcgctat taattacagc cttcaattcc ctggcctgga aagatactct gtcctgccag
     4321 aggacaacct cacagctctg ctggcctctc ctcaaacaag tgctgtcagg gacactgctc
     4381 gcagatgcag ttacgtggct tttcaccagt gtgctgaaag gcttacagat gcacgggcag
     4441 cacgacgggt gcatggcttc cctggtccat ctggccttcc agatatatga ggcactgcgc
     4501 cccaggtacc tggagataag agctgtaatg gagcaaatcc ctgaaataca gaaggactca
     4561 ctggaccagt ttgactgcaa gcttttaaac ccctccctgc agaaagtggc tgacaagcgc
     4621 cgaaaggacc aattcaaacg cctcattgct ggttgcattg ggaaaccctt gggagagcag
     4681 ttccgaaaag aagttcacat taagaatctt ccctcacttt tcaaaaaaac aaagccaatg
     4741 ctggagacgg aggtgctgga caatgatggg ggtggcctgg ccaccatctt tgaaccctga
     4801 agatcccggg ctagcgggcg gccgcttgaa ttcatcgatg gccgccatgg cccaacttgt
     4861 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     4921 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     4981 tctggatcgg gaattaattc ggcgcagcac catggcctga aataacctct gaaagaggaa
     5041 cttggttagg taccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     5101 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     5161 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5221 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     5281 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     5341 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     5401 taggcttttg caaaaagctg ttaacagctt ggcactggcc gtcgttttac aacgtcgtga
     5461 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     5521 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     5581 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     5641 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     5701 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     5761 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     5821 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
     5881 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     5941 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     6001 tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     6061 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta
     6121 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     6181 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     6241 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     6301 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     6361 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     6421 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     6481 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     6541 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     6601 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     6661 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     6721 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     6781 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     6841 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     6901 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     6961 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     7021 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     7081 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     7141 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     7201 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     7261 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     7321 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     7381 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     7441 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     7501 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     7561 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     7621 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     7681 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     7741 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     7801 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     7861 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     7921 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7981 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     8041 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     8101 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     8161 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     8221 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     8281 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
//
