LOCUS       sequence-199762-       15865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pAGM4723 containing Sr35 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15865)
  AUTHORS   Md Hatta MA, Ghosh S, Athiyannan N, Richardson T, Steuernagel B, Yu 
            G, Rouse MN, Ayliffe M, Lagudah ES, Radhakrishnan GV, Periyannan SK,
            Wulff BBH
  TITLE     Extensive Genetic Variation at the Sr22 Wheat Stem Rust Resistance 
            Gene Locus in the Grasses Revealed Through Evolutionary Genomics and
            Functional Analyses.
  JOURNAL   Mol Plant Microbe Interact. 2020 Nov;33(11):1286-1298. doi: 
            10.1094/MPMI-01-20-0018-R. Epub 2020 Oct 1.
  PUBMED    32779520
REFERENCE   2  (bases 1 to 15865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-01-20-0018-R"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-11"; volume: "33"; issue: "11"; pages: 
            "1286-1298"
COMMENT     Sequence Label: pBW_0059
FEATURES             Location/Qualifiers
     source          1..15865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1011..1356
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1299..1319
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     intron          1786..1975
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     polyA_signal    2697..2871
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             3614..3625
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    11223..11247
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11527..12156
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             12585..13658
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      13724..13918
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        13980..14084
                     /gene="bla"
                     /label=AmpR promoter
     CDS             14085..14879
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      15050..15638
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15539..15558
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    15774..15798
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga ggaattccaa tcccacaaaa atctgagctt aacagcacag
       61 ttgctcctct cagagcagaa tcgggtattc aacaccctca tatcaactac tacgttgtgt
      121 ataacggtcc acatgccggt atatacgatg actggggttg tacaaaggcg gcaacaaacg
      181 gcgttcccgg agttgcacac aagaaatttg ccactattac agaggcaaga gcagcagctg
      241 acgcgtacac aacaagtcag caaacagaca ggttgaactt catccccaaa ggagaagctc
      301 aactcaagcc caagagcttt gctaaggccc taacaagccc accaaagcaa aaagcccact
      361 ggctcacgct aggaaccaaa aggcccagca gtgatccagc cccaaaagag atctcctttg
      421 ccccggagat tacaatggac gatttcctct atctttacga tctaggaagg aagttcgaag
      481 gtgaaggtga cgacactatg ttcaccactg ataatgagaa ggttagcctc ttcaatttca
      541 gaaagaatgc tgacccacag atggttagag aggcctacgc agcaagtctc atcaagacga
      601 tctacccgag taacaatctc caggagatca aataccttcc caagaaggtt aaagatgcag
      661 tcaaaagatt caggactaat tgcatcaaga acacagagaa agacatattt ctcaagatca
      721 gaagtactat tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag
      781 agattggagt ctctaaaaag gtagttccta ctgaatctaa ggccatgcat ggagtctaag
      841 attcaaatcg aggatctaac agaactcgcc gtcaagactg gcgaacagtt catacagagt
      901 cttttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacactctg
      961 gtctactcca aaaatgtcaa agatacagtc tcagaagatc aaagggctat tgagactttt
     1021 caacaaagga taatttcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
     1081 atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga
     1141 aaggctatca ttcaagatct ctctgccgac agtggtccca aagatggacc cccacccacg
     1201 aggagcatcg tggaaaaaga agaggttcca accacgtcta caaagcaagt ggattgatgt
     1261 gacatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
     1321 tctatataag gaagttcatt tcatttggag aggacacgct cgagtataag agctcatttt
     1381 tacaacaatt accaacaaca acaaacaaca aacaacatta caattacatt tacaattatc
     1441 gatacaatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
     1501 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
     1561 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     1621 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
     1681 gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
     1741 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcaggtaaa tttctagttt
     1801 ttctccttca ttttcttggt taggaccctt ttctcttttt atttttttga gctttgatct
     1861 ttctttaaac tgatctattt tttaattgat tggttatggt gtaaatatta catagcttta
     1921 actgataatc tgattacttt atttcgtgtg tctatgatga tgatgataac tgcagccggt
     1981 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
     2041 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
     2101 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
     2161 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
     2221 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
     2281 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
     2341 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
     2401 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta
     2461 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
     2521 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
     2581 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
     2641 tcgtccgagg gcaaaggaat aggcttctct agctagagtc gatcgacaag ctcgagtttc
     2701 tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc
     2761 tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat
     2821 caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat cgctgcaagg
     2881 ttttgacggg tttactagca acatacgctg gattacgaat tcccatgggg agtgcctatg
     2941 ctctgcgctc gaggagggga gatgcttggg attgggtggg tgcttgtgag aaagagggag
     3001 taagagaaga agtaaggcca gtcaaagcaa gttcatcagg aggaggaatg gcagagagac
     3061 gatgttcaag cttactttgg ttagtggtgc aaataaaaat tctccctcat ttccaaggtt
     3121 cctttccata tttttcttct tcttaggatt cagtttctta catcttaatt aagcagcaga
     3181 tagttgagat ttgataatat tgtgtcacag tcagtgttgt gaaatattca gagattcttg
     3241 agaacaagta gttcgtctgg caaaattaat tcattcaacc atagtttgtc tcgtctggca
     3301 cacttggtta gtgctgcaaa taaaacttat cccttatttc caagcttcac tgttcagata
     3361 ttgatataat ttcttaaggt tcctgcatct taattaagaa actaccagac cataaattgt
     3421 ccacgaaaca gggcagtaat aacatctcca tcatattgga attctctgta gaaagaggaa
     3481 agtaggcaat tagtagatta tatggtagta tgatgggaaa aacaaggagc aaagtccttt
     3541 tactgttgat aacagatact agtatactta ctgttcttgg tatcattaat cccgagaaaa
     3601 ggttccacat ggtatagaag gccgaggaaa caacagatgc gatgttgtag tttggagtca
     3661 ggcccaccgt catcatcctg tagaaatgta tagtatgaca gcagggtgaa gaacatgaag
     3721 aacaggtacc ataacagctt cacagttgtc catttgaacc caatcatggc atacactagc
     3781 acgcggtata ttaacgactg aacaaagatg tatggaagtt ctattacaac ctgcaaatgg
     3841 aattacatgt taggggtcct ttgctcagtt gatagtgcgt ccacaacctc catgacctca
     3901 ttgataaaca tctgtgtacc aggtttggtg caatgaatta gttagtacaa cacacacaat
     3961 taaactgtat aaaaaggaga aaggactgac taataatctc attttaatca tgttcttcta
     4021 taatatatgc ttgtgtgcca tagtatatta tccaaattat aggacagtaa tcacctttct
     4081 tgttgaggaa acaacatcgg ccggtaatcg tagccatgca gagaatgcaa gggatatcat
     4141 tctgctcaca gtatcctgac acacgagcaa aagtttcttg cttctttgga tagtcatata
     4201 ttgtcatgtt gccctctatg tatccaccgg tctacctccc agccagcaca tccattagtt
     4261 tagtcttacc agcagcactg acacccatga gagctataat aagcacacct gacctaaatg
     4321 aaccactgat cattttcaac agtctcaacc agttctcttt cacaccttgt gctttgactt
     4381 cctgtatctt tatcagaaaa actattataa tatatcaaag gggttatttt taaatctgtt
     4441 agtatattac aggtcatatt ttgattgatg attataaaag caaatacttt gtcgagtgtt
     4501 gctgtattgc tacgccctgt tgttgtcggt gcctcttgta gaatctcacc agttagatta
     4561 gcttgtttta tcttcatcgt ttcctcaggt attgtttgct gatggctctc aaatgctgga
     4621 gggccaaatg tatagggtga aaattagacc tccagttgta attgtaagga ccctttctgt
     4681 tggcatccca agtagtacaa gttatagtaa ttcacgtttg agaagtggga gacaggtaca
     4741 atttttacct cagtaaacat tccacgccat tccagaacca gccttacaat tcggaaataa
     4801 gaaaactcaa tgggcatatg tacgtagtga atgttgcagc ttccttcgca tgcagtacgt
     4861 ctccgtaaca aggcatgctc ttttgaggtt taattttagt ttgtatgtga tatgtgatcc
     4921 gtttaactcg agctgcaatt tgcagacagt tgcgtcaagc aggaatctct tctttggttg
     4981 atcatgatgt gaacgctctt gaccatgctc agctttaaga cctaacgcag ctgtccaaca
     5041 gtgtccagtc tggctcgtac taccaagtgg taccgtagta ttgaagctgg gaccccgctg
     5101 tatacagaag cccacaggct gcacatggcc atatgccggt tatgtctccg tccggttggt
     5161 tgctacattt atctcttcct tacacccagc gaaccatcat taatctcaac cacccacaaa
     5221 acatcattaa tttcaaccca gctacacccc ttctcatcct ctccgggaca acgagagaac
     5281 acaccacgag tagagtcctt cacagaacag cacggagata ccagctttct tcctccccac
     5341 actaccgcaa tctcgcaagt ggagagagag agagagagag agagagatct agctatggag
     5401 attgccatgg gggctatcgg ctctctcctc ccgaagctcg gcgagctgct catcggcgag
     5461 atcaccctgg agaaaaaagt gagaaagggc atcgaatctc tcataacaga gctgaaattg
     5521 atgcaggctg tcctcagcaa ggtgtcgaag gtgccggcgg accagcttga tgagggggtc
     5581 aagatctggg caggaaatgt caaggagttg tcctaccaaa tggaggacat cgttgatgcc
     5641 ttcatggtgc gtgtggggga tggtggtgag tccaccaacc cgaagaacag agtcaagaag
     5701 atactcaaga aggtcaaaaa attattcaag aatggcaagg atctccatcg gatctctgct
     5761 gctctggaag aagtggttct tcaagcaaag cagttggccg agctgcgtca gaggtacgag
     5821 caagagatgc gggacactag tgctaatact agtgttgacc ctcgcatgat ggccttgtac
     5881 acagatgtga cagagctcgt cggcattgaa gaaacacgag acaagttgat caatatgttg
     5941 actgaaggtg atgattggtc gaagcatccg ttaaagacga tatctattgt tggatttggt
     6001 gggttaggca agacgactct tgccaaagca gcatacgaca agatcaaagt gcaatttgat
     6061 tgtggtgctt ttgtctcggt ttcgcgaaac cctgaaatga aaaaggtttt gaaggatatt
     6121 ctctatggac ttgacaaggt caagtacgaa aacatacata atgcagcgag ggacgaaaag
     6181 tatctcatag acgatataat tgaattcctt aatgacaaga ggtatgtatg tatgcttttt
     6241 tcctcttata cacaccgatt tccatttcat ctagtaaaat agtgacatcg tttaatacta
     6301 gtgcatatgc atcttgatag catattgtta atttatactt ttgatgtaaa tttttatagt
     6361 tattgtataa atcatataat gtaataatta gtgagtgcac atttttaagg taattggaaa
     6421 tatatattct aggtcgacga tctcaattat gtgcgtgtga gtgggggggg ggggggataa
     6481 tacaagtctc taattatgca atttggtcga gtttgcttag aagtaattta tccaaactaa
     6541 tataataata tattattttg ttatcttcat tgtattgatt tgttaactaa ttattaaatg
     6601 cattcatcaa ggtacctcat cgtaatcgat gatatatgga atgaaaaagc atgggagtta
     6661 attaagtgtg ctttctccaa gaagagcccc ggaagccgac taatcacgac aacccgaaat
     6721 gttagtgtct ctgaagcatg ttgctcttct gaggatgata tttataggat ggaacccctt
     6781 tccaatgatg tatcaagaac actcttctgt aaaagaatat tttcacaaga agaaggctgc
     6841 cctcaagaat tattgaaagt atccgaagaa attttgaaga aatgtggcgg tgtaccgtta
     6901 gctattatta ctatagcaag tctactagct aataagggtc atataaaagc aaaggatgaa
     6961 tggtatgctt tgcttagttc cattggtcat ggactcacaa aaaatcgcag tttggagcag
     7021 atgaaaaaga tattattatt cagctactat gatctacctt cttatttgaa accatgctta
     7081 ttatatctaa gcatcttccc agaggaccgc gagattagga gagctagact gatatggaga
     7141 tggatatcag aaggctttgt ttatagtgaa aagcaagata ttagcttata tgagctcggt
     7201 gacagctact tcaatgagct agtaaataga agtatgatcc agcccatagg cattgatgat
     7261 gaaggaaagg taaaggcttg ccgtgtacat gatatggtgc ttgacctcat atgctcattg
     7321 tcaagtgaag agaactttgt cacaatatta gatgacccca gaagaaaaat gcctaactca
     7381 gagagcaagg ttcgcaggtt gtccatccaa aatagcaaga tagatgtgga taccactagg
     7441 atggagcata tgagatctgt tactgttttc tcagataatg ttgttgggaa agtgttggat
     7501 atttcaagat ttaaagttct gcgcgtgttg gatttagaag gttgtcatgt ctccgatgtt
     7561 gggtatgtgg ggaatctgtt acatttgagg tacttggggc taaaaggtac tcatgttaag
     7621 gaccttccca tggaaatagg gaagctgcag tttttgctta ccttggattt aagagggact
     7681 aagatagaag tactgccgtg gagtgttgtt caactaagac gtctaatgtg cctgtatgtt
     7741 gattatggta tgaagctgcc atctgggata ggcaacctga ctttcctaga agtgctagat
     7801 gacctggggt tatctgacgt ggaccttgat tttgtgaaag aattgggccg tctgaccaag
     7861 ctcagggtgc tccggcttga cttccatggt tttgatcaga gcatgggtaa agctttggag
     7921 gaatccatta gtaatatgta caaactggac agtctagatg tatttgtcaa tcgtggactc
     7981 atcaattgct tgagcgaaca ttgggtgcct cctccacgac tctgtagatt ggctttcccg
     8041 tcaaaacgaa gttggttcaa gacattgccg tcatggatta atccttcatc gcttcctctc
     8101 ctctcctatc tggatataac gttgtttgaa gtgcgatcgg aggacatcca gcttcttggg
     8161 actctgcctg ctcttgttta tcttgagata tggaattatt ctgtgttcga agaagcgcat
     8221 gaggtggaag cgcccgtcct ttcctctggt gctgcattat tcccatgtgc gacagagtgt
     8281 cgcttcattg gtattggtgc cgtgccgtct atgtttccac aaggagctgc gccaaggctt
     8341 aaacgccttt ggttcacctt cccagccaag tggatctcca gtgaaaacat tggtttgggt
     8401 atgcggcacc tcccttccct ccagcgagtc gtcgttgacg ttatcagtga gggagctagc
     8461 cgtgaggagg cggatgaagc ggaggctgcg ctgagggccg cagccgagga ccaccccaac
     8521 cgtcccatcc tcgatatatg gtgacgcatg caaagggccg cagtggaggt acatacacta
     8581 gacgcacaag catgtattca catcatctgt acattgtcct tgatatatga tgttcattgc
     8641 tctttctttg tttcaggcgg acgaagcgag gcatgcgatt gaggttcgct agatacccct
     8701 ccacgatttt gtacaaagtg ttttcacaca aagtatttta ttcctctcct cttccgtttt
     8761 tcagtttcag gcagccggag tgtttaggct gattcgtcga atcttgcacc caggtactat
     8821 actaagtcca ttgatttgat tacaagcacc aaagtattct taatttcagc agtttcataa
     8881 gctagctagc tcgatcgatc aggcccctaa agcctaaaca ttactacgat atgaatctat
     8941 tcagcattgg cctgttcatt tatgctgaaa agatacatag gaccccttaa ttgttattcc
     9001 tgcatgctac aagattacaa ctaatctaga ttagtgagca atacactaag taatatgtgt
     9061 ttctctactt ggatgaaccc tgtgtttttt atgtatgcat ctcgacagtc caaatttgca
     9121 tgtctatcta aattctgaag tgtcaactga actgcaatgt gattttcgcc ttggttactc
     9181 tatgttgcat aagtacatag gatcagtagt tactttttct tgccacgtat aaggactcgt
     9241 attttccgaa aagacaagct ctcatatgat gtggtacaga ttagaaagaa tccactatga
     9301 agttggatga actctgtttt cttcttctgt catttaactg agccgttcac atgtggcatt
     9361 gcaggtagca tgagctaata aatttcgtcg acatggactc gatcgtgcgt gcctccaact
     9421 ccggtgccgt ttcttctcca tccaatacag tcgagcatac tgcatttgcc ttgccgtttg
     9481 acctgtcttt gttctgcaac ttcattctgt ccaccaccca tgtctgaagt ctcttcttgc
     9541 ttatgtcaat tcaacaacaa tcaagtttgt agggctcccc ctggattttg ccctgatgaa
     9601 ccattagtta tcatgtactg atgatgtctg gtttggatgc tctccagcat tgctcattac
     9661 actcctttat ttgtaacaac tttatttcag actcgatgtt tattcttatt ccagacttgt
     9721 aaactataat gtcagagttc agttcgtact atggtctgaa gtttatcttc catttgtcat
     9781 gttggacttg gtttgatcaa ataaatcatg aactggaacc tttttctctt tgctactgaa
     9841 tcacaccgtg tgcccttgct gttatttact ctgtagtttt ttttttgcga ataaactctg
     9901 tagaattatt ggccctgaac agatgcaatg gtttctggag gtaagaatgt gattcggtgt
     9961 actcccttca tcgattgtca gtctctactt tacttgcact tatacagggg agcaaaccag
    10021 gtgatgtttt ttgtgtgcca tgaagcaaat catatccttt tcgaaaaaaa tgaagcaaat
    10081 catatgctgt aacgcagagg gatgacccgt ttattagcaa ctcctgctgt ataccaccac
    10141 aataggtagc ctgcttcggc ctagctgcag ccgagatcag atgagaacgt gtaatttagc
    10201 ctgaagccgt gtaatttaac ttgtgtctaa gtctgctctg tccgaggttg cagccaagat
    10261 caggcgagat gacacatgtg tcatcccgtt tcagtctttc ttttgatcgc tcgccgtttt
    10321 gtcagaaaat cctactaact gaagctgaaa gcctgaagcc cctagctcgg tgtcacaaaa
    10381 ttcagtcaac tcttcctagt tgttcacgcc aactccaccg cgcgccccta tcctgttcgg
    10441 ccttgtctgt ttgggtaaaa cagacaaacg aggcggccca gcgcgcggga gcaaacgtac
    10501 ttttgtccgt tttgtgtccg ctttcgacac atccgcggcc caaatttgcg ccgcttttgt
    10561 ggtgaaacgg acagcacacg gacgcgcggg tcgtctatgc gtgtcctccc ctagcccgcc
    10621 cgtcggtggc acagggtggg cctttttcta tccgccccct ccctccctct ggccgcaccc
    10681 ccttcactct tcccctcgct gccgccgtcg ccgctgctgc cattgcagcc gtgcagttcg
    10741 gacgcgagct cgtccagccc cttcccctcg gcgccgtcga gctacgcaac cacggccacc
    10801 tggtgtgcgc gcccgctggc cggatttggg gcggatccgg tcggtcttga gcttggccgg
    10861 ctgtgggagg ccgggccgcg ccatggactg gtcgggcacc tcgctggaag gccctggcag
    10921 ggccgcaagg ccacatgcag gcagtggctc ctcctctagc cgctcggatc tgcaccgttg
    10981 gtggtccgcc ggcagatacg cgaatggcgg tcggccgggc tcggtgcttg cccaaaagct
    11041 cgccggcgca ccgttgccac tcattaagtg cgaccactgc ccaaggatgg tcgtgcgccg
    11101 cgtgtctaca atgccggaac atcccagatg tgtgttcatc aagtgcttaa acgacggggc
    11161 gctcagagag gatgcacatg tgaccgaggg acacgaagtg atccgtttaa actatcagtg
    11221 tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg
    11281 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc cagccgtgcg
    11341 gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt
    11401 ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag
    11461 cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg
    11521 gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc
    11581 gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc
    11641 gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt
    11701 gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc
    11761 gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc
    11821 gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg
    11881 gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc
    11941 gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg
    12001 tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc
    12061 gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag
    12121 gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga
    12181 gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa
    12241 cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg
    12301 aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc
    12361 tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga
    12421 attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg
    12481 gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc
    12541 cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac
    12601 catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg
    12661 ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc
    12721 aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgtcgt
    12781 cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg
    12841 acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc
    12901 gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt
    12961 ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt
    13021 cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg
    13081 tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc
    13141 agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc
    13201 gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta
    13261 gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag
    13321 ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc
    13381 ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg
    13441 ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg
    13501 ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc
    13561 cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc
    13621 gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc
    13681 aaattgccct agcaggggaa aaaggtcgaa aaagcttctt tcctgtggat agcacgtaca
    13741 ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt
    13801 acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt
    13861 ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac
    13921 tgtctggcca gcgcacagcc gaacagctgc aaaaagcgcc cacatcaagg ctccgagtgc
    13981 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    14041 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatggct aaaatgagaa
    14101 tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa
    14161 tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga
    14221 cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat
    14281 ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga
    14341 gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac
    14401 aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg
    14461 acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact
    14521 tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaggac actccattta
    14581 aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct
    14641 tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct
    14701 ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc
    14761 ggtcgctcag ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg
    14821 ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagc
    14881 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
    14941 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    15001 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    15061 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    15121 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    15181 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    15241 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    15301 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    15361 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    15421 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    15481 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    15541 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    15601 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    15661 tacggttcct gctcggatct gttggaccgg acagtagtca tggttgatgg gctgcctgta
    15721 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg
    15781 atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt
    15841 ttaatgtact ggggttgaac actct
//
