LOCUS       sequence-199787-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses 7Y sortase A variant in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Jeong HJ, Abhiraman GC, Story CM, Ingram JR, Dougan SK
  TITLE     Generation of Ca2+-independent sortase A mutants with enhanced 
            activity for protein and cell surface labeling.
  JOURNAL   PLoS One. 2017 Dec 4;12(12):e0189068. doi: 
            10.1371/journal.pone.0189068. eCollection 2017.
  PUBMED    29200433
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-12-4"; volume: "12(12):e0189068. doi"; pages: " 
            10.1371/journal.pone.0189068. eCollection 2017"
COMMENT     Sequence Label: pET30b-7Y-SrtA
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             491..508
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(561..579)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      575..622
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(746..765)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctagaataat tttgtttaac tttaagaagg agatatacat atgcaagcta aacctcaaat
       61 tccgaaagat aaatcaaaag tggcaggcta tattgaaatt ccagatgctg atattaaaga
      121 accagtatat ccaggaccag caacacgcga acaattaaat agaggtgtaa gctttgcaaa
      181 agaaaatcaa tcactagatg atcaaaatat ttcaattgca ggacacactt tcattgaccg
      241 tccgaactat caatttacaa atcttaaagc agccaaaaaa ggtagtatgg tgtactttaa
      301 agttggtaat gaaacacgta agtataaaat gacaagtata agaaacgtta agccaacagc
      361 tgtagaagtt ctagatgaac aaaaaggtaa agataaacaa ttaacattaa ttacttgtga
      421 tgatctgaat cgcgagacag gcgtttggga aacacgtaaa atctttgtag ctacagaagt
      481 caaactcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga
      541 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
      601 acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac
      661 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
      721 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
      781 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
      841 gctttacggc acctcgac
//
