LOCUS       sequence-199790-         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expressed 7+ sortase A variant in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Jeong HJ, Abhiraman GC, Story CM, Ingram JR, Dougan SK
  TITLE     Generation of Ca2+-independent sortase A mutants with enhanced 
            activity for protein and cell surface labeling.
  JOURNAL   PLoS One. 2017 Dec 4;12(12):e0189068. doi: 
            10.1371/journal.pone.0189068. eCollection 2017.
  PUBMED    29200433
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-12-4"; volume: "12(12):e0189068. doi"; pages: " 
            10.1371/journal.pone.0189068. eCollection 2017"
COMMENT     Sequence Label: pET30b-7+-SrtA
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             493..510
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(563..581)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      577..624
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctctagaata attttgttta actttaagaa ggagatatac atatgcaagc taaacctcag
       61 attcccaagg acaagtcgaa ggtagctggg tatattgaaa tcccagatgc ggacattaaa
      121 gaaccagttt atccaggccc ggcaactcgt gaacagctta accgtggggt atcttttgcc
      181 aaagagaatc agtcacttga cgaccagaat atctctatcg ctgggcacac gttcattgga
      241 cgcccgaact atcaattcac caatctgaag gcagccaaga agggtagtat ggtgtacttc
      301 aaagttggta atgaaactcg caaatataag atgaccagta ttcgcaatgt taaacccact
      361 gcagtggaag tcctggatga gcagaagggc aaggataagc agttgactct tattacatgc
      421 gacgatttaa accgcgaaac aggagtgtgg gaaactcgca aaattttcgt ggctacagaa
      481 gtcaaactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag
      541 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
      601 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg
      661 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      721 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttnnttcct ttctcgccac
      781 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
      841 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
      901 atcgccctga tagacggttt ttcgcccttt gacgttggag t
//
