LOCUS       sequence-199836-        2214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GLRX2MTS in domestication vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Perez-Gonzalez A, Kniewel R, Veldhuizen M, Verma HK, 
            Navarro-Rodriguez M, Rubio LM, Caro E
  TITLE     Adaptation of the GoldenBraid modular cloning system and creation of
            a toolkit for the expression of heterologous proteins in yeast 
            mitochondria.
  JOURNAL   BMC Biotechnol. 2017 Nov 13;17(1):80. doi: 
            10.1186/s12896-017-0393-y.
  PUBMED    29132331
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2017-11-13"; volume: "17(1):80. doi"; pages: " 
            10.1186/s12896-017-0393-y"
COMMENT     Sequence Label: pUPD2-GLRX2MTS (12)
FEATURES             Location/Qualifiers
     source          1..2214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1..44)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    47..68
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      234..291
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(315..332)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(486..1074)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(566..585)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1257..1351)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1372..2031)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1946..1965
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2193..2211
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
       61 ttctagagcg atgagtggtc tcgccatgga gacaaatttt tccttcgact cgaatttaat
      121 tgttattatc attatcacgt tgtttgccac aagaattatt gctaaaagat ttttatctac
      181 tccaaaaatg tcaatgtgag accactcatc gcgactagta gcggccgctg cagtccggca
      241 aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc
      301 gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      361 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      421 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      481 gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      541 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      601 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      661 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      721 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      781 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      841 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
      901 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
      961 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1021 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1081 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1141 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1201 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag gcttggattc
     1261 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
     1321 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc
     1381 gccctgccac tcgtcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
     1441 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
     1501 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
     1561 atcaaaactg gtgaaactca cccagggatt ggctgatacg aaaaacatat tctcaataaa
     1621 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
     1681 tagaaactgc cggaaatcgt cgtggtattc actccagagt gatgaaaacg tttcagtttg
     1741 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
     1801 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
     1861 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
     1921 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
     1981 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
     2041 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
     2101 atggtgaaag ttggaacctc ttacgtgccc gatcaactcg agtgccacct gacgtctaag
     2161 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg caga
//
