LOCUS       sequence-199851-         423 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for recombinant bacterial expression of SnoopLigase, a 
            protein that promotes the formation of an isopeptide bond between 2 
            peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Buldun CM, Jean JX, Bedford MR, Howarth M
  TITLE     SnoopLigase Catalyzes Peptide-Peptide Locking and Enables 
            Solid-Phase Conjugate Isolation.
  JOURNAL   J Am Chem Soc. 2018 Feb 28;140(8):3008-3018. doi: 
            10.1021/jacs.7b13237. Epub 2018 Feb 14.
  PUBMED    29402082
REFERENCE   2  (bases 1 to 423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.7b13237"; journalName: "J Am Chem Soc"; date: 
            "2018-02-28- 28"; volume: "140"; issue: "8"; pages: "3008-3018"
COMMENT     Sequence Label: pET28a-AviTag-SnoopLigase
FEATURES             Location/Qualifiers
     source          1..423
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..84
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcg gcctgaacga tatcttcgag
       61 gcccagaaga tcgagtggca cgagggtagc ggtgggtctg gtagtggtgt gaataagaac
      121 gataaaaagc cgctgcgtgg tgccgtgttt agcctgcaga aacagcatcc cgactatccc
      181 gatatctatg gcgcgattga tcagaatggg acctatcaaa atgtgcgtac cggcgaagat
      241 ggtaaactga cctttaagaa tctgagcgat ggcaaatatc gcctgtttga aaatagcgaa
      301 cccccgggct ataaaccggt gcagaataag ccgattgtgg cgtttcagat tgtgaatggc
      361 gaagtgcgtg atgtgaccag cattgtgccg ccgggtgtgc cggctacata tgaatttacc
      421 taa
//
