LOCUS       sequence-199854-         402 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for recombinant expression in bacteria of SUMO domain fused 
            to DogTag, permitting covalent linkage to SnoopTagJr.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Buldun CM, Jean JX, Bedford MR, Howarth M
  TITLE     SnoopLigase Catalyzes Peptide-Peptide Locking and Enables 
            Solid-Phase Conjugate Isolation.
  JOURNAL   J Am Chem Soc. 2018 Feb 28;140(8):3008-3018. doi: 
            10.1021/jacs.7b13237. Epub 2018 Feb 14.
  PUBMED    29402082
REFERENCE   2  (bases 1 to 402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.7b13237"; journalName: "J Am Chem Soc"; date: 
            "2018-02-28- 28"; volume: "140"; issue: "8"; pages: "3008-3018"
COMMENT     Sequence Label: pET28a-SUMO-DogTag
FEATURES             Location/Qualifiers
     source          1..402
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..33
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             331..399
                     /codon_start=1
                     /product="peptide derived from the RrgA adhesin of 
                     Streptococcus pneumoniae"
                     /label=DogTag
                     /note="forms a covalent bond with the DogCatcher protein 
                     when inserted into an intra-domain protein loop (Keeble et 
                     al., 2022)"
                     /translation="DIPATYEFTDGKHYITNEPIPPK"
ORIGIN
        1 atgggtagct ggagccatca ccatcatcat cacgggagcg atagcgaagt gaaccaggaa
       61 gcgaaaccgg aagttaaacc ggaagtgaaa ccggaaaccc atattaatct gaaagttagc
      121 gacggcagca gcgaaatctt ttttaaaatt aaaaaaacca ccccgctgcg tcgcctgatg
      181 gaagcctttg cgaaacgtca gggtaaagaa atggatagcc tgcgctttct gtatgacggc
      241 atccgtattc aggccgatca gaccccggaa gacctggata tggaagacaa cgatattatt
      301 gaagcgcatc gcgaacagat cggtggtggc gatattccgg ctacatatga atttaccgat
      361 ggtaaacatt atatcaccaa tgaaccgata ccgccgaaat aa
//
