LOCUS       sequence-199880-        2820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty vector with attL5-attL2 recombination sites for Gateway 
            Cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Lowder LG, Zhou J, Zhang Y, Malzahn A, Zhong Z, Hsieh TF, Voytas DF,
            Zhang Y, Qi Y
  TITLE     Robust transcriptional activation in plants using multiplexed 
            CRISPR-Act2.0 and mTALE-Act systems.
  JOURNAL   Mol Plant. 2017 Nov 29. pii: S1674-2052(17)30344-1. doi: 
            10.1016/j.molp.2017.11.010.
  PUBMED    29197638
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2017 Nov 29. pii: S1674-2052(17)30344-1. doi: 
            10.1016/j.molp.2017.11.010."
COMMENT     Sequence Label: pYPQ140
FEATURES             Location/Qualifiers
     source          1..2820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(19..118)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(135..154)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(136..154)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(159..175)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(159..175)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             606..1397
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1490..2078
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1979..1998
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2232..2249
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2408..2435)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2527..2613)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2662..2684
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2676..2693
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2677..2693
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2709..2804
                     /gene="mutant version of attL"
                     /label=attL5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ctagtaaggg cgaattcgac ccagctttct tgtacaaagt tggcattata aaaaataatt
       61 gctcatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
      121 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
      181 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
      241 gcctcctctg gaccagccag gacagaaatg cctcgacttc gctgctgccc aaggttgccg
      301 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
      361 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
      421 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
      481 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
      541 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
      601 acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
      661 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
      721 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
      781 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
      841 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
      901 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
      961 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     1021 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     1081 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     1141 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     1201 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     1261 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     1321 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     1381 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     1441 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1501 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1561 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1621 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     1681 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     1741 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     1801 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     1861 tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     1921 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     1981 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2041 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2101 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2161 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     2221 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     2281 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     2341 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tcgccaggaa gagtttgtag
     2401 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     2461 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     2521 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     2581 ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt
     2641 taacgcttgc atggatgttt tcccagtcac gacgttgtaa aacgacggcc agtcttaagc
     2701 tcgggcccaa ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
     2761 tgagcaatgc ttttttataa tgccaacttt gtatacaaaa gttgccccat ggcgttccct
//
