LOCUS       sequence-199891-        1601 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mouse monomeric Rev7 and fragment of Champ1 protein (amino
            acids 328-355) from bicistronic plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Thanos Halazonetis
  TITLE     Expression plasmids for production of Rev7 and Champ1 proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pT5T_Rev7_1-211_SA112_KVK_Champ1_328-355
FEATURES             Location/Qualifiers
     source          1..1601
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(227..232,233..249,250..255))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     329..348
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        329..347
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             379..401
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             409..441
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     RBS             1121..1143
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1317..1335)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1331..1378
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1423..1441)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg
       61 ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg
      121 cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata
      181 cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aaacttatcg
      241 atgataagct gtcaaacatg agaattgagc tccgcggaga tccttagcga aagctaagga
      301 ttttttttag atctcgatcc cgcgaaatta atacgactca ctatagggag accacaacgg
      361 tttccctcta gaaataattt tgtttaactt taagaaggag atatacatat ggctagcatg
      421 actggtggac agcaaatggg tcgggatcca ttggaggatg attaaatgac caccctcacg
      481 cgccaagacc tcaactctgc ccaagtggtg gctgacgtgc tctccgagtt cctggaggtg
      541 gccgtgcacc tgattctcta tgtgcgcgag gtctacccgg tgggcatctt ccagaagcgc
      601 aagaagtaca acgtgccggt tcagatgtcc tgtcacccgg agctgaacca gtacatccag
      661 gacacactcc actgcgtcaa acctctcctg gagaagaacg atgtggagaa ggtggtggtg
      721 gtgattttgg ataaggaaca ccgcccagtg gagaagtttg tctttgagat cactcagcct
      781 cccttgctgt ccatcaattc agactccctc ctgtctcatg tggagcagct gcttcgagcc
      841 ttcatcctta agattagtaa ggttgataag gtcctggatc acaaccctcc aggctgcaca
      901 tttacagtcc tcgtgcacac aagagaagct gctactcgaa acatggagaa gatacaggtc
      961 atcaaggact tcccgtggat tctggcagat gaacaggatg tccacatgca cgacccccgc
     1021 ttgatacccc taaaaaccat gacgtcggac attttaaaga tgcagctcta cgttgaagag
     1081 cgagcgcata agaacagcta acccgggctc tagaaataat tttgtttaac tttaagaagg
     1141 agatatacca tgtcagcatc atcaggacct tggaagccag ctaaacctgc tccatctgtg
     1201 tctcctggac cttggaaacc aattccttct gtatcttaag cggccgcagc ttggtaccga
     1261 ctagtaagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
     1321 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
     1381 gaaaggagga actatatccg gataattctt gaagacgaaa gggcctcgtg atacgcctat
     1441 ttttataggt taatggcatg ataataatgg gttcttaaga cgtcaggtgg gcacttttcg
     1501 gggaaaatgt gcgcggaacc ctatttggtt atttttctaa atacattcaa atatgtatcc
     1561 gctcatgagc acaataaccc tgataaatgc ttcaataata t
//
