LOCUS       sequence-199960-        3576 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pTEI071
FEATURES             Location/Qualifiers
     source          1..3576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     188..207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     441..458
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1903..1921)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1989..2093
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2313..3104
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3275..3576,1..287)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
       61 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      121 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      181 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      241 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      301 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      361 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      421 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      481 gcaaaccgcc tctccccgcg cgttggccga ttcattaatc actctgtggt ctcaggagtc
      541 tgaagctcgt aacattggct catacgatat ctggataaca atatatcaca ttgctctgat
      601 accatattaa aatcacatcc acgcattgaa tggactgatc atatatctta catattagtt
      661 aaaatccatt gtcgtgcgag agattgcctt ccgtgacatc ctctgtggac caggttcgct
      721 tgtcacacta gctataattg gtgaacacat gataacatat atgtttggag catagaagag
      781 ctagcgcctg tgtcattggt gtgtggtatc gaaagaacaa gagaatagaa caggagaaca
      841 tgaatatagc taggtttatt cattttcacc ccaaagctta gaaattgcgt tccaaagagt
      901 gggtgaattc atgtgtgagg gcaattagtg attgtaaaaa taaaattgtg ttttgtaaaa
      961 aacttttact gtcgaaatta tttagggtga tgaaaaaatc agtaaactac gaatgatagc
     1021 ttaaagagtt tctatcaaag tgattgagga atagtttgtt gcaaattaaa cctctaacaa
     1081 aatgttttct gttgtggttt ttcatctcta caaattttga attttatgat gaattagaaa
     1141 gatagaatga gttactttag attttaaaag gttgttcaag tttacaaaac agattactag
     1201 aatcatgatt aaaaatttac aagctacata ttgtctaaac caatgatgtt gaacatacca
     1261 gatgatagtt tttcagtgtt tgaacaatca attggatagt ttttatgttt ctgcaaaata
     1321 tgcaaataat cagtgttttt gagtctttgc attttgattt aaaagcaaaa acaactgagt
     1381 ttcaaggtta aattaattac attattcatg agatttatca ggttagtgga taaactgaca
     1441 atggaatcaa tgttattgta aattggtagt gatgttggac ttctaatgtt actctctatg
     1501 atgtttcggt catcaatatc acactatctt tacttttatt taaaggaaag atcacacaaa
     1561 taagttatct ctattcagaa ctattaagct gcttccaaaa gacttgcaac atgtggactc
     1621 gaaatgcttt ggctgcaatg aaaaaatcat agcaaaagct agtggactag agactgccac
     1681 ataagaatag taaacgttaa aaccaaaatc tcaaaaatcc aatgagtaaa gagatataga
     1741 ttacttcata gataacaaac gttactcgca attttcctat ataatccaac cctacctaac
     1801 cattttcaat cactctcact cacaagttag tcaccaaaaa aaaaaaaaaa cacaaaaagt
     1861 ttcaatgtga gaccacgaag tggctcttca gtggacgaaa gggcctcgtg atacgcctat
     1921 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     1981 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     2041 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgcgct
     2101 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     2161 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     2221 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     2281 agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat
     2341 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     2401 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagc gatattgatt
     2461 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     2521 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     2581 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     2641 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     2701 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     2761 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     2821 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     2881 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     2941 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     3001 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     3061 tccattacgt aaaaggcgag atcaccaagg tagtcggcaa ataactgtca gaccaagttt
     3121 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     3181 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3241 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3301 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3361 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3421 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3481 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3541 ccgggttgga ctcaagacga tagttaccgg ataagg
//
