LOCUS       sequence-19997-e         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Kraynov VS, Chamberlain C, Bokoch GM, Schwartz MA, Slabaugh S, Hahn 
            KM
  TITLE     Localized Rac activation dynamics visualized in living cells.
  JOURNAL   Science. 2000 Oct 13. 290(5490):333-7.
  PUBMED    11030651
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2000 Oct 13. 290(5490):333-7."
COMMENT     Sequence Label: pET23-PBD(65-150)-N-Cys
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             327..349
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(380..399)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(381..399)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tttcgggctt tgttagcagc cggntctcag tggtggtggt ggtggtgctc gagagctgac
       61 ttatctgtaa agctcatgta tttctggctg ttggatgtct tcttcgagtt gtaaaactcc
      121 aacacatcca gaacagcctg cgggtttttc ttctgctccg acttagtgat atttgatgtc
      181 tgaagcaagc gggcccactg ctctggcatt cccgtaaact cccctgtgac agcatcaaaa
      241 ccgacatgaa ttgtgtgttc aaaatctgaa gggagagaaa tctctggccg ctctttctct
      301 ttcttcttat tgccgcacat ggtatatctc cttcttaaag ttaaacaaaa ttatttctag
      361 agggaaaccg ttgtggtctc cctatagtga gtcgtattaa tttcgcggga tcgagatctc
      421 gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg
      481 gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac
      541 gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg
      601 tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttantgtncc
//
