LOCUS       sequence-199973-        6817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bos taurus Arcn1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Arakel EC, Richter KP, Clancy A, Schwappach B
  TITLE     delta-COP contains a helix C-terminal to its longin domain key to 
            COPI dynamics and function.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):6916-21. doi: 
            10.1073/pnas.1603544113. Epub 2016 Jun 13.
  PUBMED    27298352
REFERENCE   2  (bases 1 to 6817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1603544113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-21- 21"; volume: "113"; issue: "25"; pages: "6916-21"
COMMENT     Sequence Label: pRS303 Bt delta-COP
FEATURES             Location/Qualifiers
     source          1..6817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2187..2209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2206..2222
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2243..2261
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4753..4772)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4754..4772)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4779..4796)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4779..4795)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4788..4810)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4936..5391
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5023..5042)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5233..5254
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5651..6313)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(6314..6501)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(6648..6667)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6767..6789
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctc gaaattaacc ctcactaaag ggaacaaaag ctggtaccgg
     2281 gccccccctc gagagtaatc atcaaatcat tcaaatagat gtcattttga atggataagt
     2341 caggtattaa aaggttattc ttgataagtt cgatcttgat agggggcaag agcttctcac
     2401 acgcggcata atcgccgttt tcaaaggcta tgcttaacga cttggtcaat tcggctaacg
     2461 agggcattac gatggcttct tatctcactc caatagtact ttccaccggt tatacttccg
     2521 gcttttccct attaatacaa gctacaattt caatgggtgg caaataatgt gtagaataga
     2581 aaataagccg acagggtaat aaagaaaatt tttagaaaaa aaaggttaga tggcttattt
     2641 aagttacagg ctagcgaaaa aaggaacttc agggcaagta aagtgtttga ttgggcacta
     2701 gcatggctta taaaggcgag caattgtcga aactaattaa tgttgtacgg actattgctg
     2761 tcttctcgtg gtaaatgcgt gttccaggtc gaatactact tgcacacagg cgagcggggc
     2821 cccataaaag tgttgccgat ttgttaagtt gtcttttcgg tttttctact ctgttattcc
     2881 ttacttccct ttttaagaac tctttttatc cttcatttag gatcttgcac gtctccgcct
     2941 catcacttga attaaaacat gtctctgtca gtaaaccttg gcgtttctat tgttcttcat
     3001 agttcaactt ttattattac ccgccctgcg cgtttacatt tttccagcaa cagccagcga
     3061 aaaattagaa aatctggttg ttgacacctc aagaacaagg gcaattagcc tcagcgtcga
     3121 atatagatca tattagaata cctatagctc catcaaaaga aatacacagg atccaccatg
     3181 gtgctgttgg cagcagcagt ctgtacaaaa gcaggaaaag ctattgtttc tcgacagttt
     3241 gtagagatga ctcgaactcg gattgagggc ttgttagcag cttttccaaa gctcatgaac
     3301 actggaaaac aacatacatt tgtggaaaca gagagtgtca gatatgttta ccagcctatg
     3361 gagaaattgt acatggtact gatcaccacc aaaaacagca acatcttaga agatctggag
     3421 accctaaggc tcttctcaag agtgattcct gaatattgcc gagccttaga agagaatgaa
     3481 atatctgagc actgttttga tttgattttt gcatttgacg aaattgttgc cttgggatac
     3541 cgggagaatg ttaacctggc acagatcaga accttcacag aaatggattc ccatgaggag
     3601 aaggtgttca gagcagtcag agagactcaa gagcgtgaag ccaaggctga gatgcggcgt
     3661 aaagcaaaag aattacagca ggcccgaaga gatgcagaga gacagggcaa aaaagcacca
     3721 ggatttgggg gctttggaag ctccacagtg tctggaggca gcacaacttc tatgatcaca
     3781 gagaccatca ttgaaactga caaaccaaaa gtggcacccg caccagccag accttcaggc
     3841 cctagcaagg ctctgaaact gggagccaaa ggaaaggaag tagataactt cgttgacaaa
     3901 ttgaaatctg aaggcgaaaa tatcatatcc tccaatatgg gcaagcgtac ctctgaagca
     3961 accaaagtgc atgctccacc cattaatatg gagagtgtgc acatgaagat tgaggaaaag
     4021 atcacattaa cctgtggccg agatggagga ttacagaaca tggagctgca cggcatgatc
     4081 atgcttagga tctcagatga taaatttggc cgaattcgtc ttcatgtgga aaatgaagac
     4141 aagaaaggag tacagctaca gacccatcca aatgtggata aaaaactttt cactgcagaa
     4201 tctctaattg gtttgaagaa tccagagaag tcatttccag tcaacagtga tgtaggggtg
     4261 ctaaagtgga gactgcagac cacagaggag tcttttattc cactgacaat taattgctgg
     4321 ccctcagaaa gtggaaatgg ttgtgatgtc aacatagaat atgagctaca agaagataat
     4381 ttagaactga atgatgtggt tatcaccatt ccactcccat ctggtgttgg cgctccagtg
     4441 attggtgaga ttgatggcga gtatcgacat gacagtcgac gaaatacctt ggagtggtgc
     4501 ctgccagtga ttgatgccaa aaataagagt ggcagccttg agttcagcat tgctgggcag
     4561 cccaatgact tcttccctgt tcaagtctcc ttcatctcca aaaaaaatta ctgtaacata
     4621 caggttacca aagtgaccca ggtagatgga aacagccctg taaggttttc cacagagacc
     4681 actttcctag tggataagta tgaaattctg tagataagaa tgcggccgcc accgcggtgg
     4741 agctccaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt
     4801 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     4861 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     4921 ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4981 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     5041 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     5101 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     5161 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5221 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     5281 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     5341 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt tcctgatgcg
     5401 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagatccgt cgagttcaag
     5461 agaaaaaaaa agaaaaagca aaaagaaaaa aggaaagcgc gcctcgttca gaatgacacg
     5521 tatagaatga tgcattacct tgtcatcttc agtatcatac tgttcgtata catacttact
     5581 gacattcata ggtatacata tatacacatg tatatatatc gtatgctgca gctttaaata
     5641 atcggtgtca ctacataaga acacctttgg tggagggaac atcgttggta ccattgggcg
     5701 aggtggcttc tcttatggca accgcaagag ccttgaacgc actctcacta cggtgatgat
     5761 cattcttgcc tcgcagacaa tcaacgtgga gggtaattct gctagcctct gcaaagcttt
     5821 caagaaaatg cgggatcatc tcgcaagaga gatctcctac tttctccctt tgcaaaccaa
     5881 gttcgacaac tgcgtacggc ctgttcgaaa gatctaccac cgctctggaa agtgcctcat
     5941 ccaaaggcgc aaatcctgat ccaaaccttt ttactccacg cacggcccct agggcctctt
     6001 taaaagcttg accgagagca atcccgcagt cttcagtggt gtgatggtcg tctatgtgta
     6061 agtcaccaat gcactcaacg attagcgacc agccggaatg cttggccaga gcatgtatca
     6121 tatggtccag aaaccctata cctgtgtgga cgttaatcac ttgcgattgt gtggcctgtt
     6181 ctgctactgc ttctgcctct ttttctggga agatcgagtg ctctatcgct aggggaccac
     6241 cctttaaaga gatcgcaatc tgaatcttgg tttcatttgt aatacgcttt actagggctt
     6301 tctgctctgt catctttgcc ttcgtttatc ttgcctgctc attttttagt atattcttcg
     6361 aagaaatcac attactttat ataatgtata attcattatg tgataatgcc aatcgctaag
     6421 aaaaaaaaag agtcatccgc taggtggaaa aaaaaaaatg aaaatcatta ccgaggcata
     6481 aaaaaatata gagtgtacta gaggaggcca agagtaatag aaaaagaaaa ttgcgggaaa
     6541 ggactgtgtt atgacttccc tgactaatgc cgtgttcaaa cgatacctgg cagtgactcc
     6601 tagcgctcac caagctctta aaacgggaat ttatggtgca ctctcagtac aatctgctct
     6661 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     6721 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     6781 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcga
//
