LOCUS       sequence-199979-       10174 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus expressing eGFP and optimised Rabies Glycoprotein (oG).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10174)
  AUTHORS   Ciabatti E, Gonzalez-Rueda A, Mariotti L, Morgese F, Tripodi M
  TITLE     Life-Long Genetic and Functional Access to Neural Circuits Using 
            Self-Inactivating Rabies Virus.
  JOURNAL   Cell. 2017 Jul 1. pii: S0092-8674(17)30697-9. doi: 
            10.1016/j.cell.2017.06.014.
  PUBMED    28689641
REFERENCE   2  (bases 1 to 10174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2017 
            Jul 1. pii: S0092-8674(17)30697-9. doi: 10.1016/j.cell.2017.06.014."
COMMENT     Sequence Label: pLenti-CMV-GFP-2A-oG-WPRE
FEATURES             Location/Qualifiers
     source          1..10174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(140..159)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     487..508
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             560..625
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     misc_feature    2249..2837
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2302..2322)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2720..2731)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2924..3157
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3229..3350
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3266..3285)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3320..3339
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3390..3525
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3545..3564)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3546..3564)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3574..3591)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3574..3590)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3583..3605)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3732..4187
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3819..3838)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4029..4050
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4213..4317
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4318..4386,4387..5178)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4536..4555)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5349..5937
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5838..5857
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6091..6108
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6225..6246
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6261..6266,6267..6284,6285..6291)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6299..6315
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6304..6326
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6323..6339
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6323..6339
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6358..6378
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6360..6378
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        6479..6858
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6859..7062
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7077..7257
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7304..7429
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7922..8155
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8340..8384
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8651..8768
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        8869..9172
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9173..9376
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          9525..9903
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and 
                     human beta-globin intron 2"
     CDS             join(10011..10013,10014..10174,1..550)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(10053..10074)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
       61 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      121 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      181 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      241 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
      301 caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcag
      361 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
      421 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
      481 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactcacg gcatggacga
      541 gctgtacaag ggatccggag tgaaacagac tttgaatttt gaccttctca agttggcggg
      601 agacgtggag tccaacccag ggcccatggt cccacaggct ctcctcttcg tccctctgct
      661 cgtcttccca ctctgctttg gtaaattccc tatctacact atccccgaca agctgggccc
      721 ctggagtcct atcgatattc accatctgtc atgccctaac aatctcgtgg tcgaggacga
      781 aggctgtacc aacctgagcg gattctccta catggagctg aaagtgggct atatctctgc
      841 tattaagatg aacgggttca catgcactgg tgtggtcacc gaggcagaaa cctacacaaa
      901 ttttgtggga tatgtcacca caactttcaa gagaaaacac tttcggccaa caccagacgc
      961 ctgtcgggca gcttacaact ggaagatggc tggcgacccc cgctatgagg aaagcctgca
     1021 caatccttac ccagactatc attggctgcg cactgtgaag accacaaaag agagcctggt
     1081 catcatttcc ccatctgtcg ccgacctgga cccctacgac cgatctctcc acagtccagt
     1141 gtttcccggc ggaaactgct ccggagtggc cgtcagctcc acttactgtt ccaccaacca
     1201 tgattataca atctggatgc ctgagaatcc aaggctgggg atgtcctgcg acatcttcac
     1261 caacagcagg ggcaagaggg cctcaaaagg cagcgagact tgtggatttg tggatgaaag
     1321 agggctgtat aagagcctca aaggtgcctg caagctgaaa ctctgtggcg tgctgggact
     1381 ccggctgatg gacggaacct gggtcgctat gcagacatct aacgagacta agtggtgccc
     1441 ccctgggcag ctcgtgaatc tgcacgactt ccgaagcgat gagatcgaac atctggtggt
     1501 cgaggaactc gtgaagaaaa gggaggaatg tctcgatgct ctggagtcaa ttatgactac
     1561 caagagcgtg agcttcagga ggctgagcca cctgaggaag ctcgtccctg gcttcggaaa
     1621 agcatacacc atctttaaca agacactgat ggaggcagac gcccattata aatctgtgag
     1681 aacctggaat gaaatcattc ctagtaaggg atgcctgcga gtcgggggtc gatgtcaccc
     1741 acatgtgaac ggggtcttct ttaatggtat cattctgggg ccagatggta acgtgctgat
     1801 ccccgagatg cagtctagtc tgctccagca gcacatggaa ctgctcgtgt caagcgtcat
     1861 tcccctgatg catcctctcg cagacccaag cacagtgttc aaaaacggcg acgaggccga
     1921 agattttgtg gaggtccacc tgcccgacgt gcacgaacgg atcagtggcg tcgatctcgg
     1981 actgcctaat tggggcaagt acgtgctgct ctccgctgga gcactgactg ctctcatgct
     2041 gatcattttc ctgatgacct gctggcgacg agtgaaccgc tccgagccaa ctcagcacaa
     2101 tctgaggggg accggtagag aagtgtctgt cacaccccag agcggtaaaa tcatcagcag
     2161 ttgggaaagt cataaatctg ggggcgagac acggctctaa tgaaagcttg cctcgaggat
     2221 ggggactgat aaaagcttcg agctcgacaa tcaacctctg gattacaaaa tttgtgaaag
     2281 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     2341 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     2401 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     2461 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     2521 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     2581 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     2641 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     2701 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     2761 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     2821 ttgggccgcc tccccgcctg gaattcgagc tcggtacctt taagaccaat gacttacaag
     2881 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     2941 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     3001 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     3061 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     3121 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     3181 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     3241 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     3301 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     3361 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3421 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     3481 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     3541 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     3601 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     3661 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     3721 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     3781 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     3841 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     3901 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     3961 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4021 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     4081 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4141 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     4201 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     4261 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     4321 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     4381 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     4441 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     4501 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     4561 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     4621 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     4681 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     4741 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     4801 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     4861 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     4921 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     4981 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     5041 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     5101 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     5161 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     5221 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5281 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5341 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5401 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5461 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     5521 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5581 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5641 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     5701 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     5761 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     5821 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5881 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     5941 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     6001 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     6061 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     6121 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     6181 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     6241 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     6301 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     6361 attaaccctc actaaaggga acaaaagctg gagctgcaag cttggccatt gcatacgttg
     6421 tatccatatc ataatatgta catttatatt ggctcatgtc caacattacc gccatgttga
     6481 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     6541 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     6601 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     6661 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     6721 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     6781 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     6841 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     6901 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     6961 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     7021 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccggggt
     7081 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     7141 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     7201 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     7261 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     7321 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     7381 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     7441 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     7501 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     7561 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     7621 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     7681 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     7741 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     7801 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     7861 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     7921 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     7981 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     8041 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     8101 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     8161 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     8221 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     8281 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     8341 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     8401 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     8461 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     8521 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     8581 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt
     8641 atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     8701 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     8761 aaaattttat cgatcacgag actagcctcg actctagaaa gacgcgtcta gttattaata
     8821 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     8881 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     8941 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     9001 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     9061 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     9121 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     9181 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     9241 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     9301 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     9361 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
     9421 ttttgacctc catagaagac accgggaccg atccagcctc cgcggattcg aatcccggcc
     9481 gggaacggtg cattggaacg cggattcccc gtgccaagag tgacgtaagt accgcctata
     9541 gagtctatag gcccacaaaa aatgctttct tcttttaata tacttttttg tttatcttat
     9601 ttctaatact ttccctaatc tctttctttc agggcaataa tgatacaatg tatcatgcct
     9661 ctttgcacca ttctaaagaa taacagtgat aatttctggg ttaaggcaat agcaatattt
     9721 ctgcatataa atatttctgc atataaattg taactgatgt aagaggtttc atattgctaa
     9781 tagcagctac aatccagcta ccattctgct tttattttat ggttgggata aggctggatt
     9841 attctgagtc caagctaggc ccttttgcta atcatgttca tacctcttat cttcctccca
     9901 cagctcctgg gcaacgtgct ggtctgtgtg ctggcccatc actttggcaa agaattggga
     9961 ttcgaacatc gattgaattc ggtacccttc aggtcgactc cggagccacc atgagcaagg
    10021 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
    10081 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
    10141 tgaagttcat ctgcaccacc ggcaagctgc ccgt
//
