LOCUS       sequence-199994-        4194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human IFITM3-F63Q with an N-terminal myc tag in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Chesarino NM, Compton AA, McMichael TM, Kenney AD, Zhang L, Soewarna
            V, Davis M, Schwartz O, Yount JS
  TITLE     IFITM3 requires an amphipathic helix for antiviral activity.
  JOURNAL   EMBO Rep. 2017 Oct;18(10):1740-1751. doi: 10.15252/embr.201744100. 
            Epub 2017 Aug 23.
  PUBMED    28835547
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embr.201744100"; journalName: "EMBO Rep"; date: "2017-10";
            volume: "18"; issue: "10"; pages: "1740-1751"
COMMENT     Sequence Label: myc-hIFITM3-F63Q
FEATURES             Location/Qualifiers
     source          1..4194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     52..74
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     66..83
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     67..83
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        110..413
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        414..617
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     568..588
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     614..638
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          755..851
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    1416..1550
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1440..1459)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1494..1513
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1661..1677)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1661..1677)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1674..1696)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1685..1701
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1709..1715,1716..1733,1734..1739))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1754..1775
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1892..1909)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2063..2651)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2143..2162)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2822..3613,3614..3682))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3445..3464
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3683..3787)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3855..3873
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3911..3933)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4033..4052
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
       61 gacgttgtaa aacgacggcc agtgagttcg agcttgcatg cctgcaggtc gttacataac
      121 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      181 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      241 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      301 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      361 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      421 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      481 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      541 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      601 tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
      661 gttttgacct ccatagaaga caccgggacc gatccagcct ccggactcta gaggatccgg
      721 tactagagga actgaaaaac cagaaagtta actggtaagt ttagtctttt tgtcttttat
      781 ttcaggtccc ggatccggtg gtggtgcaaa tcaaagaact gctcctcagt ggatgttgcc
      841 tttacttcta ggcctgtacg gaagtgttac ttctgctcta aaagctgcgg aattgtaccc
      901 gcgggcccac catggcatca atgcagaagc tgatctcaga ggaggacctg cttatggcca
      961 tggaggcccg aattcccatg aatcacactg tccaaacctt cttctctcct gtcaacagtg
     1021 gccagccccc caactatgag atgctcaagg aggagcacga ggtggctgtg ctgggggcgc
     1081 cccacaaccc tgctcccccg acgtccaccg tgatccacat ccgcagcgag acctccgtgc
     1141 ccgaccatgt cgtctggtcc ctgcagaaca ccctcttcat gaacccctgc tgcctgggct
     1201 tcatagcatt cgcctactcc gtgaagtcta gggacaggaa gatggttggc gacgtgaccg
     1261 gggcccaggc ctatgcctcc accgccaagt gcctgaacat ctgggccctg attctgggca
     1321 tcctcatgac cattctgctc atcgtcatcc cagtgctgat cttccaggcc tatggatagg
     1381 tcgaccgaga tctctcgagg taccgcggcc gcggggatcc agacatgata agatacattg
     1441 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     1501 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     1561 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt tcggatcctc
     1621 tagagtcgat ctgcaggcat gctagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     1681 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     1741 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     1801 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     1861 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     1921 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     1981 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2041 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2101 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2161 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2221 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2281 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2341 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2401 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2461 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     2521 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     2581 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2641 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     2701 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     2761 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     2821 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     2881 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     2941 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     3001 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3061 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     3121 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     3181 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     3241 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     3301 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     3361 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     3421 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     3481 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     3541 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     3601 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     3661 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     3721 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     3781 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     3841 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     3901 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     3961 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     4021 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     4081 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
     4141 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctgg
//
