LOCUS       sequence-199999-        4194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human IFITM3-S61A,N64A,T65A with an N-terminal myc tag in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Chesarino NM, Compton AA, McMichael TM, Kenney AD, Zhang L, Soewarna
            V, Davis M, Schwartz O, Yount JS
  TITLE     IFITM3 requires an amphipathic helix for antiviral activity.
  JOURNAL   EMBO Rep. 2017 Oct;18(10):1740-1751. doi: 10.15252/embr.201744100. 
            Epub 2017 Aug 23.
  PUBMED    28835547
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embr.201744100"; journalName: "EMBO Rep"; date: "2017-10";
            volume: "18"; issue: "10"; pages: "1740-1751"
COMMENT     Sequence Label: myc-hIFITM3-S61A,N64A,T65A
FEATURES             Location/Qualifiers
     source          1..4194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     59..81
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     73..90
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     74..90
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        117..420
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        421..624
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     575..595
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     621..645
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          762..858
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    1423..1557
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1447..1466)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1501..1520
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1668..1684)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1668..1684)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1681..1703)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1692..1708
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1716..1722,1723..1740,1741..1746))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1761..1782
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1899..1916)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2070..2658)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2150..2169)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2829..3620,3621..3689))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3452..3471
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3690..3794)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3862..3880
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3918..3940)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4040..4059
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
       61 cagtcacgac gttgtaaaac gacggccagt gagttcgagc ttgcatgcct gcaggtcgtt
      121 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      181 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      241 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      301 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      361 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      421 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
      481 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      541 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      601 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      661 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg gactctagag
      721 gatccggtac tagaggaact gaaaaaccag aaagttaact ggtaagttta gtctttttgt
      781 cttttatttc aggtcccgga tccggtggtg gtgcaaatca aagaactgct cctcagtgga
      841 tgttgccttt acttctaggc ctgtacggaa gtgttacttc tgctctaaaa gctgcggaat
      901 tgtacccgcg ggcccaccat ggcatcaatg cagaagctga tctcagagga ggacctgctt
      961 atggccatgg aggcccgaat tcccatgaat cacactgtcc aaaccttctt ctctcctgtc
     1021 aacagtggcc agccccccaa ctatgagatg ctcaaggagg agcacgaggt ggctgtgctg
     1081 ggggcgcccc acaaccctgc tcccccgacg tccaccgtga tccacatccg cagcgagacc
     1141 tccgtgcccg accatgtcgt ctgggccctg ttcgccgccc tcttcatgaa cccctgctgc
     1201 ctgggcttca tagcattcgc ctactccgtg aagtctaggg acaggaagat ggttggcgac
     1261 gtgaccgggg cccaggccta tgcctccacc gccaagtgcc tgaacatctg ggccctgatt
     1321 ctgggcatcc tcatgaccat tctgctcatc gtcatcccag tgctgatctt ccaggcctat
     1381 ggataggtcg accgagatct ctcgaggtac cgcggccgcg gggatccaga catgataaga
     1441 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     1501 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     1561 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttcg
     1621 gatcctctag agtcgatctg caggcatgct agcttggcgt aatcatggtc atagctgttt
     1681 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     1741 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     1801 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     1861 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     1921 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1981 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2041 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2101 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2161 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2221 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2281 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2341 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2401 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2461 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     2521 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2581 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2641 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2701 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2761 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2821 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2881 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2941 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     3001 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3061 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3121 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3181 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3241 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3301 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3361 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3421 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3481 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3541 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3601 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3661 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3721 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3781 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     3841 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     3901 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     3961 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     4021 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     4081 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
     4141 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgc
//
