LOCUS       sequence-200036-       13228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP-Kif26b in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13228)
  AUTHORS   Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantu AV, Choi SS, Jong 
            BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R,
            Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH
  TITLE     Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the 
            control of cell and tissue behaviors in vertebrates.
  JOURNAL   Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509.
  PUBMED    28885975
REFERENCE   2  (bases 1 to 13228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-09-8"; volume: "6. pii: e26509. doi"; pages: " 
            10.7554/eLife.26509"
COMMENT     Sequence Label: pEF5-FRT-V5-DEST-GFP-Kif26b
FEATURES             Location/Qualifiers
     source          1..13228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(42..61)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        349..1527
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          580..1518
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1475..1495
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        1544..1562
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1641..1665
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1693..1695,1696..1698,1699..2409)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1693..1695,1696..1698,1699..2409)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1738..1759)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1999..2018)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2346..2367
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8798..8932
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(9022..9046)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9099..9140
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(9178..9195)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9184..9408
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9541..9560)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    9692..9739
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             9747..10769
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             9747..10769
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    10899..11020
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11069..11085)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11069..11085)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11082..11104)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11093..11109
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11117..11123,11124..11141,11142..11147))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11162..11183
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11300..11317)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11471..12059)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11551..11570)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12230..13021,13022..13090))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12853..12872
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13091..13195)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
       61 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
      121 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
      181 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
      241 ttattgacta ggcttttgca aaaagctttg caaagatgga taaagtttta aacagagagg
      301 aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc
      361 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
      421 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
      481 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
      541 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc
      601 cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg
      661 ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg
      721 ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
      781 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
      841 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
      901 gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac
      961 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
     1021 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
     1081 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
     1141 gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg
     1201 ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc
     1261 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc
     1321 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     1381 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     1441 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     1501 ttttttcttc catttcaggt gtcgtgagga attagcttgg tactaatacg actcactata
     1561 gggagaccca agctggctag gtaagcttgg taccgagctc ggatccacta gtccagtgtg
     1621 gtggaattct gcagatatca acaagtttgt acaaaaaagc aggctggcgc cggaaccaat
     1681 tcagtcgaca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     1741 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     1801 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     1861 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     1921 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     1981 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     2041 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     2101 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     2161 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     2221 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     2281 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     2341 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     2401 ctgtacaagg gccggcctac catgaattcg gtagccggaa ataaagagag gcttgcggtc
     2461 tccaccaggg gcaagaaata cggggtgaac gaaatgtgct cgcccaccaa gccctcagcg
     2521 ccctgctctc ctgagagctg gtaccgcaag gcttacgagg agtcgcgcgc cggcagccgg
     2581 cccaccccgg agggagcagg ctctgcgctg ggctcatcgg gcaccccgtc cccgggctcg
     2641 ggcacctcgt ccccgagctc cttcaccggc tctccgggac ctgcatctcc gggcattggt
     2701 accagctcgc ccggctctct gggcggctca cccggcttcg gcaccggctc ccctggctcg
     2761 ggcagcggcg gcggctcctc ccccggctcc gaccgcggcg tctggtgcga gaactgtaac
     2821 gcccgcttgg tggagctgaa gaggcaggct ctcaagctgc tgctgccggg acccctcccg
     2881 ggaaaggacc ctgctttctc tgctgtgatt cacgacaaac tccaggttcc caatactatc
     2941 aggaagtcgt ggaatgaccg agacaaccgc tgtgacattt gtgccacaca cctgaaccag
     3001 ctaaagcagg aagccatcca aatggtgctg accttggagc aggcagctgg cagcgagcac
     3061 tatgacacct ctctgggctc accccctccg atctccagca ttcccacact ggtggggtct
     3121 cgccacatgg gtgggctcca gcagcccagg gagtgggcct ttgtgcctgc cccctatgcc
     3181 acttctacct acacaggcct tgtcaacaaa cacagcggca aacccaacag ccttggggtc
     3241 agcaacggag cggaaaagaa gagtggctcc ccaacccacc aggccaaggt cagcctccag
     3301 atggccacca gtcccagcaa tggaaacatc ctcaactcgg tggccattca ggctcatcaa
     3361 tatctagacg gcacctggtc cctttcaaga accaatgggg ttaccctgta cccctaccag
     3421 atctcccagc tgatgacaga gaccggccgc gagggactga cagagtcagc gctcaaccgc
     3481 tacaatgctg acaagcctgc agccagcagt gtcccagccc cacagggttc ctgtgtggcc
     3541 agtgagactt ccacagggac ctcagtggcc gcttccttct tcgcacgagc tgcccagaag
     3601 ttaaatctgt catctaagaa gaagaaacac cggccttcga ctccatctgt tgcggaggct
     3661 cccctctttg cgaccagctt cagtgggatc ctgcagacct ctcctccccc agccccaccc
     3721 tgtctcctga gggctgtcaa caaggtgaag gacactcctg gcatgggcaa ggtgaaagtc
     3781 atgctgcgta tctgttccac atcagctcga gatacctcag aatccagctc tttcctgaag
     3841 gtggacccac ggaagaagca gatcaccttg tatgatcccc tgacttgcgg aggtcaaaat
     3901 gccttccaga aaagaagcag ccaggttcct ccgaagatgt ttgccttcga tgctgttttc
     3961 ccacaagatg cttctcaggc tgaagtttgt gctggaactg tggccgaagt catccagtct
     4021 gtggtgaacg gggcagatgg ctgtgtgttc tgttttggcc atgccaagct agggaaatcc
     4081 tacaccatga tcggaaggga cgactctatg cagaacctgg gcattattcc atgtgctatt
     4141 tcttggctgt tcaagcttat caatgagcgc aaagagaaga cgggggcccg attctcagtc
     4201 cgcatctcag ccgttgaggt gtggggaaaa gaggagaacc ttcgagacct gttgtctgag
     4261 gtggccacgg gcagcctaca ggacgggcag tccccaggcg tgtacctctg tgaagacccc
     4321 atctgtggca cacagctgca gaaccagagt gagctccggg cccccaccgc agagaaagct
     4381 gccttcttcc tggacgccgc cattgcctcg aggcgaagca accaacagga ttgtgacgag
     4441 gatgaccacc gcaactccca tatgctcttc accctgcaca tctaccagta ccggatggag
     4501 aagagcggga agggtggaat gtctggaggt cgaagccgcc tgcatctcat cgacctcggg
     4561 agctgtgtga aagctctcag caaaaaccga gaaggaggct cggggctgtg cctctcactg
     4621 tctgctctgg gcaacgtcat cctggctttg gtcaacggga gcaagcacat cccatacaag
     4681 gagagcaagc tcaccatgct tctgagggag tctctgggga atgtgaactg tcgcaccacc
     4741 atgatcgcac acatctcggc cgcagccagc agctacgcgg agaccctgtc caccatccag
     4801 atcgcgtcga gagtcttaag gatgaagaag aagaagacca agtacacatc aagctcctct
     4861 gggggagaaa gctcctgtga ggaaggcaga atgcgcaggc ccacccagct gagacccttc
     4921 catgcccggg ctccggtgga tccagaattc ccccttgccc cactgtccag tgatcctgac
     4981 tactcctcca gcagtgagca gtcctgcgac accgtcatct acatcgggcc caatggcaca
     5041 gccctgtctg acaaggaact cactgacaat gaggggcccc cagactttgt gcccattgtg
     5101 ccagccctac agaagaccag aggtgacagc cggccggggg aggcagcaga ggctgcagcc
     5161 agcaagtcag aaagggactg tctgaagtgc aatacgtttg ctgagctgca ggaaagacta
     5221 gattgcatag atggcagcga agaacccagt aagtttccct tcgaagagct gcccatccag
     5281 ttcgggccgg agcaagctgg cagatgtgct cctttaagcc aagcagtggg gccagacaca
     5341 ccctctgagt ctgaaaagga agataacggc tcagacaatg gccaattgac cgagagggaa
     5401 ggcacagagc ttccagcctc caaggcacag cggaatcggt cccctgcccc tactgtgaca
     5461 cgcagcagca gccccagtcc agcctcacca cggagcatcc cgggcagcag tagccagcac
     5521 agtacctccc agctcacaca gagccccagt ctccagagca gccgggagag tctgaactca
     5581 tgtggcttcg tggagggcaa gcccagaccc atgggctccc cgaggctagg cattgccagt
     5641 ctgtccaaga cctcagagta caaacctccc agttctccct ctcagaggtg caaagtctac
     5701 acccagaagg gggtcctgcc ctcctctgcc ccaccacctt ccctgagcaa ggactctggc
     5761 atggtctcca gtgaatcctt gcttcagccc gacgtgcgca ctcccccagt tggaatgagt
     5821 ccccaggttt tgaagaaatc catgtctgcc gggagcgaag ggtttcccgg aactctagtt
     5881 gacggtgagg accaggaagg ccctccagcg gactctaaga aggagattct gagcaccaca
     5941 atggtgactg tgcaacagcc cctggagctg aatggtgagg atgagttggt cttcacactg
     6001 gtggaggagc tgaccatcag cggggtgctc gacagtggac gccctaccag catcatcagc
     6061 ttcaacagtg actgctccgt gcaggccctg gcctcaggct cccggcccgt cagcatcatc
     6121 agcagcatca gcgaggatct ggagtgctac tccagcatgg cccccgtctc cgaggtgagc
     6181 atcacgcagt tcctgcctct cccaaagctg ggcctggatg agaaggctcg ggacgcgggc
     6241 agccggcgtt cctccatcag ctcctggctg agcgagatga gcgcgggcag tgatggcgag
     6301 cagtcatgcc acagcttcat agcccagacg tgctttgggc atggggaagc catggcagaa
     6361 cccccagcct cggagtttgt cagcagcatc cagaacacgg cagtggtgtg cagagagaag
     6421 cccgaggtgg gccctgacaa cctgctcatc ttatctgaga tgggggaaga gtctggcaac
     6481 aaagccgctc ccatcaaagg ctgcaaaatc tcaacggtgg gcaaggccat ggtcaccatc
     6541 tccaacacag ccagcctgag cagctgcgaa gggtacatcc ccatgaagac caacatcacc
     6601 gtttacccct gcatcgccat gagcccccga aacgtgcaag agcctgagtc ctccactgcc
     6661 accccaaagg tgagccccaa agcatcccag gcccaggaaa gcaaggaacc cagtacaagg
     6721 agagagatga aatttgagga tccttggctt aaacgagaag aagaggtgaa aagggagaac
     6781 gcttactcca gcgaggaagg ggttaagtgt gagccgctcc caagctccct gaagactgaa
     6841 ggtaagtcag agcaggaact ggatggcagg cccagctcag gcaacaggct gagcagcagc
     6901 agctcagagg cagccgcctt tcaagggact gataacgtta ggagagtcgt ggatggctgt
     6961 gagatggccc tgcctggctt ggtggcccaa agtcccctcc atgtcaaccg aaacctaaag
     7021 tcaagcagcc tcccaagggc atttcagaaa gctgacaggc acgaggagct ggacagcttc
     7081 taccattgcc tggcagacag caatggcttc agtgctgctt cgggcatccc atcctcgaag
     7141 acaaccctag agaggaaagt tgcttcaccc aagcattgcg tcctggcaag gcccaaaggg
     7201 accccgcctc tgcctcctgt ccgcaagtct agcttggacc agaagaacag ggccagccct
     7261 caacacggtg gtggcagcag caacaccagc agccccctga accagccagc taccttcttg
     7321 gcttgcttcc ctgatgagtc caacggcaag actaaggatg tcagtagcag cagtaagctc
     7381 ttcagcgcca agctggaaca gctggccagc agaagcaact ctcttggcag gacaacagtc
     7441 agccactacg aatgcttgtc gttggagagg gccgaaagcc tgtcgtcggt gagctcccgc
     7501 atgcatgccg gcaaagatag taccatgccc cgcactggga ggagcctggg tcgcagcact
     7561 ggcgcctcac cacccagctg tggcatcact cagtccacag gggcctctcc caaggccagc
     7621 cagtctaaga tctctgcagt gagcaagctc ctgctggcca gtcctaaatc ccgcagcagc
     7681 ctgtccacct ccaccaccaa aaccctcagc ttctccacca agtccctgcc acagtctgtg
     7741 ggccagagct ccaacctacc gcccagcggg aaacacatgt cctggtccac ccagtcgctc
     7801 agcaggaacc ggggctctgg gctggcttcc aaactgccgc taagagcggt caacgggcgc
     7861 atttcggagc tgctccaggg cagtgcgggc ccgaggagtg cacagctgcg ggcagaggcg
     7921 gaagagcgca gtggggctcc ctcggaggac aagcccgcag ccgcccacct gctgccctcg
     7981 ccctacagca agatcacccc tccgaggaag ccgcaccgct gcagcagcgg gcacggtagt
     8041 gataacagca gtgtcctgag tggggagcta ccacctgcca tggggaagac tgccctcttc
     8101 taccacagcg gaggcagcag tggctacgag agcatgatga gagacagcga ggccaccggc
     8161 agtgcatcct cagcccagga ctccatgagc gagaacagca gctccgtggg agggagatgc
     8221 aggagcctca aaaaccaaaa gaaaaggtcc aattcaggtt cccagagacg gaggctcatt
     8281 ccagcactgt ctctcgatac cccctcacct gtgaggaaaa ctgccagcag cacaggggtc
     8341 cgctgggtgg acggcccctt gaggagcacc cagaggagcc ttggggaacc attcgaaatt
     8401 aaagtctatg agattgatga cgtggagcgc ctgcagcgac gtcgaggagc tacaagcaag
     8461 gaggtcatgt gcttcaatgc caagctgaaa atcctagaac atcggcagca gagaatcgct
     8521 gaagtccgag ccaagtacga gtggctgatg aaggagctag aagcgaccaa gcagtacctg
     8581 atgctggacc ccaacaagtg gcttcgtgaa tttgacttgg agcaggtcct gcagctggat
     8641 tccctggagt acctggaggc actggagggt gtgacagagc gcttggagag ccgcgtcaac
     8701 ttctgcaagg cccatctcat gatgatcacc tgctttgaca tcacctccag gcgccgataa
     8761 ggcgcgcctc tagaactata gtgagtcgta ttacgtagat ccagacatga taagatacat
     8821 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     8881 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     8941 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaattcgc
     9001 ggccgcactc gagatatcta gacccagctt tcttgtacaa agtggttgat atccagcaca
     9061 gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc
     9121 tcctcggtct cgattctacg cgtaccggtt agtaatgagt ttaaacccgc tgatcagcct
     9181 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     9241 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     9301 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     9361 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     9421 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     9481 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     9541 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     9601 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     9661 aacttgatta gggtgatggt tcacgtacct agaagttcct attccgaagt tcctattctc
     9721 tagaaagtat aggaacttcc ttggccaaaa agcctgaact caccgcgacg tctgtcgaga
     9781 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     9841 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     9901 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     9961 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
    10021 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
    10081 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
    10141 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
    10201 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
    10261 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
    10321 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
    10381 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
    10441 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
    10501 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
    10561 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
    10621 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
    10681 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
    10741 ccagcactcg tccgagggca aaggaatagc acgtactacg agatttcgat tccaccgccg
    10801 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
    10861 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
    10921 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    10981 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
    11041 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
    11101 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
    11161 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
    11221 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
    11281 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    11341 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    11401 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    11461 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    11521 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    11581 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    11641 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    11701 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    11761 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    11821 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    11881 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
    11941 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    12001 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    12061 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    12121 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    12181 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    12241 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    12301 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    12361 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    12421 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    12481 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    12541 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    12601 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    12661 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    12721 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    12781 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    12841 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    12901 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    12961 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    13021 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    13081 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    13141 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    13201 ttccccgaaa agtgccacct gacgtcga
//
