LOCUS       sequence-200043-        8223 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Chimaera 7 (COPD-Ret2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8223)
  AUTHORS   Arakel EC, Richter KP, Clancy A, Schwappach B
  TITLE     delta-COP contains a helix C-terminal to its longin domain key to 
            COPI dynamics and function.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):6916-21. doi: 
            10.1073/pnas.1603544113. Epub 2016 Jun 13.
  PUBMED    27298352
REFERENCE   2  (bases 1 to 8223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1603544113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-21- 21"; volume: "113"; issue: "25"; pages: "6916-21"
COMMENT     Sequence Label: p415 CHI-7
FEATURES             Location/Qualifiers
     source          1..8223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(169..188)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     288..310
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(348..366)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    408..911
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        948..1052
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1053..1121,1122..1913)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1271..1290)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2084..2672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2573..2592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2826..2843
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2960..2981
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2996..3001,3002..3019,3020..3026)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3034..3050
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3039..3061
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3058..3074
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3058..3074
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3093..3113
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3095..3113
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3164..3513
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     primer_bind     3514..3530
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3514..3530
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5168..5184)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5169..5185)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      5187..5434
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5203..5221)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(5452..5471)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5453..5471)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5481..5498)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5481..5497)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5490..5512)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5638..6093
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5725..5744)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5935..5956
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6394..6803
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             6804..7898
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 taaaaaaaaa aaaatacaac aaataaaaaa cactcaatga cctgaccatt tgatggagtt
       61 taagtcaata ccttcttgaa ccatttccca taatggtgaa agttccctca agaattttac
      121 tctgtcagaa acggccttaa cgacgtagtc gatatggtgc actctcagta caatctgctc
      181 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
      241 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
      301 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
      361 cctattttta taggttaatg tcatgataat aatggtttct taggacggat cgcttgcctg
      421 taacttacac gcgcctcgta tcttttaatg atggaataat ttgggaattt actctgtgtt
      481 tatttatttt tatgttttgt atttggattt tagaaagtaa ataaagaagg tagaagagtt
      541 acggaatgaa gaaaaaaaaa taaacaaagg tttaaaaaat ttcaacaaaa agcgtacttt
      601 acatatatat ttattagaca agaaaagcag attaaataga tatacattcg attaacgata
      661 agtaaaatgt aaaatcacag gattttcgtg tgtggtcttc tacacagaca agatgaaaca
      721 attcggcatt aatacctgag agcaggaaga gcaagataaa aggtagtatt tgttggcgat
      781 ccccctagag tcttttacat cttcggaaaa caaaaactat tttttcttta atttcttttt
      841 ttactttcta tttttaattt atatatttat attaaaaaat ttaaattata attattttta
      901 tagcacgtga tgaaaaggac ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta
      961 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1021 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     1081 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     1141 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     1201 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     1261 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     1321 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     1381 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     1441 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     1501 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     1561 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     1621 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     1681 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     1741 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     1801 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     1861 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     1921 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     1981 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     2041 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2101 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2161 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2221 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2281 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2341 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     2401 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     2461 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     2521 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     2581 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     2641 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     2701 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     2761 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     2821 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     2881 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     2941 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     3001 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     3061 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     3121 agctggagct ccggatgcaa gggttcgaat cccttagctc tcattatttt ttgctttttc
     3181 tcttgaggtc acatgatcgc aaaatggcaa atggcacgtg aagctgtcga tattggggaa
     3241 ctgtggtggt tggcaaatga ctaattaagt tagtcaaggc gccatcctca tgaaaactgt
     3301 gtaacataat aaccgaagtg tcgaaaaggt ggcaccttgt ccaattgaac acgctcgatg
     3361 aaaaaaataa gatatatata aggttaagta aagcgtctgt tagaaaggaa gtttttcctt
     3421 tttcttgctc tcttgtcttt tcatctacta tttccttcgt gtaatacagg gtcgtcagat
     3481 acatagatac aattctatta cccccatcca tactctagaa ctagtggatc catggtgctg
     3541 ttggcagcag cagtctgtac aaaagcagga aaagctattg tttctcgaca gtttgtagag
     3601 atgactcgaa ctcggattga gggcttgtta gcagcttttc caaagctcat gaacactgga
     3661 aaacaacata catttgtgga aacagagagt gtcagatatg tttaccagcc tatggagaaa
     3721 ttgtacatgg tactgatcac caccaaaaac agcaacatct tagaagatct ggagacccta
     3781 aggctcttct caagagtgat tcctgaatat tgccgagcct tagaagagaa tgaaatatct
     3841 gagcactgtt ttgatttgat ttttgcattt gacgaaattg ttgccttggg ataccgggag
     3901 aatgttaacc tggcacagat cagaaccttc acagaaatgg attcccatga ggagaaggtg
     3961 ttcagagcag tcagagagac tcaagagcgt gaagccaagg ctgagatgcg gcgtaaagca
     4021 aaagaattac agcaggcccg aagagatgca gagagacagg gcaaaaaagc accaggattt
     4081 gggggctttg gaagctccac agtgtctgga ggcagcacaa cttctatgat cacagagacc
     4141 atcattgaaa ctgacaaacc aaaagtggca cccgcaccag ccagaccttc aggccctagc
     4201 aaggctctga aactgggagc caaaggaaag gaagtagata acttcgttga caaattgaaa
     4261 tctgaaggcg aaaatatcat atcctccaat atgggcaagc gtacctctga agcaaccaaa
     4321 gtgcatgctc cacccattaa tatggagagt gtgcacatga agattgagga aaagatcaca
     4381 ttaacctgtg gccgagatgg aggattacag aacatggagc tgcacggcat gatcatgctt
     4441 aggatctcag atgataaatt tggccgaatt cgtcttcatg tggaaaatga agacaagaaa
     4501 ggagtacagc tacagaccca tccaaatgtg gataaacaat cgttcctatc cactaaactt
     4561 atctccctgc gtgataaatc taaggctttc ccagccaacg accaaagcct gggtgttcta
     4621 agatggcgta aagtcgctcc cgcagaagat gactcattaa ttccattaac tctaactaca
     4681 tgggtttccc cctccgaatc acaacagggc tttgatgtca ttattgagta tgagagcgtc
     4741 ctggaaacag aactcgctga tgtgatcttc accattccag ttttccctca ggagccagta
     4801 gacataaata cagagtcttc tacatgctct gacgctgaag ttgtcaacat ggaccaagaa
     4861 atgggaacct ccattaagat tagcaaaatt gctgccaacg atgcaggtgc gctagcattc
     4921 actatcgaag ctccatatga agatgcattg tacccaatga ccgtttcctt ccaagagtcc
     4981 acgagggata aattagctaa gagtttcacc ggtatggcca tacaatctgt tgtcatggcc
     5041 aatgaccacg atcaagagct cccttacgac gtcatcacct ctttgaaatc agatgaatat
     5101 cttgtccaat aggcggccgc tctagaacta gtgcccccgg gctgcaggaa ttcgatatca
     5161 agcttatcga taccgtcgac ctcgagtcat gtaattagtt atgtcacgct tacattcacg
     5221 ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt
     5281 ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat ttcaaatttt
     5341 tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga
     5401 gaaggttttg ggacgctcga aggctttaat ttgcggccgg tacccaattc gccctatagt
     5461 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     5521 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     5581 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggacg
     5641 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     5701 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     5761 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     5821 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     5881 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     5941 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     6001 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     6061 cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc tccttacgca
     6121 tctgtgcggt atttcacacc gcatatcgac cggtcgagga gaacttctag tatatctaca
     6181 tacctaatat tattgcctta ttaaaaatgg aatcccaaca attacatcaa aatccacatt
     6241 ctcttcaaaa tcaattgtcc tgtacttcct tgttcatgtg tgttcaaaaa cgttatattt
     6301 ataggataat tatactctat ttctcaacaa gtaattggtt gtttggccga gcggtctaag
     6361 gcgcctgatt caagaaatat cttgaccgca gttaactgtg ggaatactca ggtatcgtaa
     6421 gatgcaagag ttcgaatctc ttagcaacca ttattttttt cctcaacata acgagaacac
     6481 acaggggcgc tatcgcacag aatcaaattc gatgactgga aattttttgt taatttcaga
     6541 ggtcgcctga cgcatatacc tttttcaact gaaaaattgg gagaaaaagg aaaggtgaga
     6601 gcgccggaac cggcttttca tatagaatag agaagcgttc atgactaaat gcttgcatca
     6661 caatacttga agttgacaat attatttaag gacctattgt tttttccaat aggtggttag
     6721 caatcgtctt actttctaac ttttcttacc ttttacattt cagcnanata tatatatata
     6781 tatttcaagg atataccatt ctaatgtctg cccctaagaa gatcgtcgtt ttgccaggtg
     6841 accacgttgg tcaagaaatc acagccgaag ccattaaggt tcttaaagct atttctgatg
     6901 ttcgttccaa tgtcaagttc gatttcgaaa atcatttaat tggtggtgct gctatcgatg
     6961 ctacaggtgt tccacttcca gatgaggcgc tggaagcctc caagaaggct gatgccgttt
     7021 tgttaggtgc tgtgggtggt cctaaatggg gtaccggtag tgttagacct gaacaaggtt
     7081 tactaaaaat ccgtaaagaa cttcaattgt acgccaactt aagaccatgt aactttgcat
     7141 ccgactctct tttagactta tctccaatca agccacaatt tgctaaaggt actgacttcg
     7201 ttgttgtcag agaattagtg ggaggtattt actttggtaa gagaaaggaa gacgatggtg
     7261 atggtgtcgc ttgggatagt gaacaataca ccgttccaga agtgcaaaga atcacaagaa
     7321 tggccgcttt catggcccta caacatgagc caccattgcc tatttggtcc ttggataaag
     7381 ctaatgtttt ggcctcttca agattatgga gaaaaactgt ggaggaaacc atcaagaacg
     7441 aattccctac attgaaggtt caacatcaat tgattgattc tgccgccatg atcctagtta
     7501 agaacccaac ccacctaaat ggtattataa tcaccagcaa catgtttggt gatatcatct
     7561 ccgatgaagc ctccgttatc ccaggttcct tgggtttgtt gccatctgcg tccttggcct
     7621 ctttgccaga caagaacacc gcatttggtt tgtacgaacc atgccacggt tctgctccag
     7681 atttgccaaa gaataaggtc aaccctatcg ccactatctt gtctgctgca atgatgttga
     7741 aattgtcatt gaacttgcct gaagaaggta aggccattga agatgcagtt aaaaaggttt
     7801 tggatgcagg tatcagaact ggtgatttag gtggttccaa cagtaccacc gaagtcggtg
     7861 atgctgtcgc cgaagaagtt aagaaaatcc ttgcttaaaa agattctctt tttttatgat
     7921 atttgtacat aaactttata aatgaaattc ataatagaaa cgacacgaaa ttacaaaatg
     7981 gaatatgttc atagggtaga cgaaactata tacgcaatct acatacattt atcaagaagg
     8041 agaaaaagga ggatgtaaag gaatacaggt aagcaaattg atactaatgg ctcaacgtga
     8101 taaggaaaaa gaattgcact ttaacattaa tattgacaag gaggagggca ccacacaaaa
     8161 agttaggtgt aacagaaaat catgaaacta tgattcctaa tttatatatt ggaggatttt
     8221 ctc
//
