LOCUS       sequence-20006-e         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Wu EW, Clemens KE, Heck DV, Munger K
  TITLE     The human papillomavirus E7 oncoprotein and the cellular 
            transcription factor E2F bind to separate sites on the 
            retinoblastoma tumor suppressor protein.
  JOURNAL   J Virol. 1993 Apr . 67(4):2402-7.
  PUBMED    8445736
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1993 Apr . 67(4):2402-7."
COMMENT     Sequence Label: pGEX2T E7 N245
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..28
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(384..406)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(551..569)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcaancggat ctggttccgc gtggatccat gggtggaggt atgcctacat tgcatgaata
       61 tatgttagat ttgcaaccag agacaactga tctctactgt tatgagcaat taaatgacag
      121 ctcagaggag gaggatgaaa tagatggtcc agctggacaa gcagaaccgg acagagccca
      181 ttacaatatt gtaacctttt gttgcaagtg tgactctacg cttcggttgt gcgtacaaag
      241 cacacacgta gacattcgta ctttggaaga cctgttaatg ggcacactag gaattgtgtg
      301 ccccatctgt tctcagaaac cataagaatt catcgtgact gactgacgat ctgcctcgcg
      361 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
      421 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
      481 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta taattcttga
      541 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
      601 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaaccnntnt nttntnnnnn
      661 nnnctaaata ctttaaaatt tgnttnccct cncngngaaa caataaacnn nnntaaagtg
      721 ncttcaatan tttgaaaaag gaaaganatg gantatccac acttttnngg gggncntttt
      781 tcnttntttt tnngnggggt tttt
//
