LOCUS       sequence-200064-       10341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Fzd4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10341)
  AUTHORS   Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantu AV, Choi SS, Jong 
            BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R,
            Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH
  TITLE     Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the 
            control of cell and tissue behaviors in vertebrates.
  JOURNAL   Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509.
  PUBMED    28885975
REFERENCE   2  (bases 1 to 10341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-09-8"; volume: "6. pii: e26509. doi"; pages: " 
            10.7554/eLife.26509"
COMMENT     Sequence Label: pLEX307-mFzd4
FEATURES             Location/Qualifiers
     source          1..10341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        91..1269
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          322..1260
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1217..1237
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1289..1313
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3021..3155
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3245..3269)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3277..3318
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3369..3957
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3422..3442)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3840..3851)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4029..4262
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4340..4461
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4501..4636
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4657..4675)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4685..4702)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4685..4701)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4694..4716)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4843..5298
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4930..4949)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5140..5161
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5324..5428
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5429..5497,5498..6289)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5647..5666)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6460..7048
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6949..6968
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7202..7219
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7336..7357
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7372..7377,7378..7395,7396..7402)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7410..7426
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7415..7437
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7434..7450
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7434..7450
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7469..7489
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7471..7489
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7517..7743
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7744..7924
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7971..8096
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8589..8822
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9007..9051
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9282..9492
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9304..9500
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9351..9486
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9532..10134
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9532..9551)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10028..10048
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10177..10294
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcctgcagg tgcaaagatg gataaagttt taaacagaga ggaatctttg cagctaatgg
       61 accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc
      121 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
      181 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
      241 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
      301 gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt
      361 acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt
      421 gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc
      481 ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc
      541 tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt
      601 tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat
      661 ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag
      721 cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag
      781 tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc
      841 tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc
      901 ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag
      961 tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg
     1021 gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct
     1081 ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact
     1141 gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt
     1201 ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag
     1261 gtgtcgtgag gctagcatcg attgatcaac aagtttgtac aaaaaagcag gctggcgccg
     1321 gaaccaattc agtcgactgg atccggtacc gaattcaggc cggcctacca tggcctggcc
     1381 gggcacaggg ccgagcagcc ggggggcgcc tggaggcgtc gggctcaggc tggggctgct
     1441 gctgcagttc ctcctgctcc tgcggccgac actggggttc ggggacgagg aggagcggcg
     1501 ctgcgacccc atccgcatcg ccatgtgcca gaacctcggc tacaacgtga ccaagatgcc
     1561 caacttagtg ggacacgagc tgcagacaga cgccgagctg cagctgacaa ctttcacgcc
     1621 gctcatccag tacggctgct ccagccagct gcagttcttc ctttgttcgg tttatgtgcc
     1681 aatgtgcaca gagaagatca acatccccat cggcccgtgc ggtggcatgt gcctttcagt
     1741 caagagacgc tgtgaaccag tcctgagaga atttgggttt gcctggcccg acaccctgaa
     1801 ctgcagcaag ttcccgcccc agaacgacca caaccacatg tgcatggaag gaccaggtga
     1861 tgaagaggtt cccttgcccc acaagactcc catccagccc ggggaagagt gccactccgt
     1921 gggaagcaat tctgatcagt acatctgggt gaagaggagc ctgaactgtg ttctcaagtg
     1981 tggctacgat gctggcttgt acagccgctc agctaaggag ttcacggata tttggatggc
     2041 tgtgtgggcc agcctctgct tcatctccac caccttcacc gtgctgacct tcctgattga
     2101 ttcatccagg ttttcttacc ctgagcgccc catcatattt ctcagtatgt gctataatat
     2161 ttatagcatt gcttatattg ttcggctgac tgtaggccgg gaaaggatat cctgtgattt
     2221 tgaagaggcg gcagagcccg ttctcatcca agaaggactt aagaacacag gatgtgcaat
     2281 aattttcttg ctgatgtact tttttggaat ggccagctcc atttggtggg ttattctgac
     2341 actcacttgg tttttggcag ccggactcaa gtggggtcat gaagccattg aaatgcacag
     2401 ttcttatttc cacatcgcag cctgggctat tcccgcagtg aaaaccattg tcatcttgat
     2461 tatgagacta gtggatgccg atgaactgac tggcttgtgc tatgttggga accaaaacct
     2521 agatgccctc actggctttg tggtggctcc tctctttacg tatttggtga ttggaacgct
     2581 gttcattgcg gcgggtttgg tggccttatt caaaattcgg tccaatcttc aaaaagacgg
     2641 gacaaagaca gacaagttgg aaaggctaat ggtcaagatc ggggtcttct cagtactgta
     2701 cacggttcct gcaacctgtg tgattgcctg ttatttctat gaaatctcaa actgggcact
     2761 ctttcgatat tctgcagatg actcaaacat ggcagttgaa atgttgaaaa tttttatgtc
     2821 tttgctcgtg ggcatcactt caggcatgtg gatttggtct gccaaaactc ttcacacgtg
     2881 gcaaaagtgt tctaaccgat tggtgaattc tgggaaggta aagagagaga agagggggaa
     2941 tggttgggtg aagccaggaa aaggcaacga gactgtggta taaggcgcgc ctctagaact
     3001 atagtgagtc gtattacgta gatccagaca tgataagata cattgatgag tttggacaaa
     3061 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     3121 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     3181 tgtttcaggt tcagggggag gtgtgggagg ttttttaatt cgcggccgca ctcgagatat
     3241 ctagacccag ctttcttgta caaagtggtt gatatcggta agcctatccc taaccctctc
     3301 ctcggtctcg attctacgta gtaatgaact agtaccggtt aagtcgacaa tcaacgcgtt
     3361 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     3421 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     3481 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     3541 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     3601 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     3661 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     3721 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     3781 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     3841 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     3901 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc
     3961 gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     4021 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     4081 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     4141 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     4201 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     4261 cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     4321 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     4381 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     4441 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     4501 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     4561 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     4621 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     4681 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     4741 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     4801 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     4861 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     4921 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4981 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     5041 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     5101 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     5161 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     5221 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     5281 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     5341 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     5401 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     5461 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     5521 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     5581 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     5641 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     5701 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     5761 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5821 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     5881 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     5941 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6001 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     6061 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     6121 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     6181 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6241 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6301 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6361 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6421 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6481 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6541 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6601 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     6661 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     6721 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6781 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6841 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6901 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     6961 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7021 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7081 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     7141 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     7201 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     7261 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     7321 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     7381 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     7441 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     7501 ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg
     7561 atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa
     7621 gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga
     7681 cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata
     7741 aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7801 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7861 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7921 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     7981 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     8041 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     8101 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     8161 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     8221 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     8281 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     8341 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     8401 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     8461 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     8521 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     8581 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     8641 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     8701 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     8761 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     8821 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     8881 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     8941 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     9001 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     9061 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     9121 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     9181 tcaccattat cgtttcagac ccacctccca accccgaggg gacccatgca ttgcatctca
     9241 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     9301 gcatgcgtct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     9361 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     9421 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     9481 gaggcctagg cttttgcaaa aagctttcta gaggtaccac cggagcttac catgaccgag
     9541 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     9601 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     9661 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     9721 gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa
     9781 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     9841 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     9901 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     9961 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
    10021 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
    10081 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag ttaacgaatt
    10141 ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
    10201 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
    10261 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
    10321 tccactttgg cgccggctcg a
//
