LOCUS       sequence-200073-       10446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Fzd7 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10446)
  AUTHORS   Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantu AV, Choi SS, Jong 
            BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R,
            Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH
  TITLE     Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the 
            control of cell and tissue behaviors in vertebrates.
  JOURNAL   Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509.
  PUBMED    28885975
REFERENCE   2  (bases 1 to 10446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-09-8"; volume: "6. pii: e26509. doi"; pages: " 
            10.7554/eLife.26509"
COMMENT     Sequence Label: pLEX307-mFzd7
FEATURES             Location/Qualifiers
     source          1..10446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        91..1269
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          322..1260
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1217..1237
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1289..1313
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3126..3260
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3350..3374)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3382..3423
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3474..4062
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3527..3547)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3945..3956)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4134..4367
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4445..4566
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4606..4741
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(4762..4780)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4790..4807)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4790..4806)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4799..4821)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4948..5403
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5035..5054)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5245..5266
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5429..5533
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5534..5602,5603..6394)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5752..5771)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6565..7153
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7054..7073
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7307..7324
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7441..7462
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7477..7482,7483..7500,7501..7507)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7515..7531
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7520..7542
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7539..7555
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7539..7555
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7574..7594
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7576..7594
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7622..7848
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7849..8029
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8076..8201
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8694..8927
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9112..9156
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9387..9597
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9409..9605
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9456..9591
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9637..10239
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9637..9656)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10133..10153
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10282..10399
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcctgcagg tgcaaagatg gataaagttt taaacagaga ggaatctttg cagctaatgg
       61 accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc
      121 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
      181 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
      241 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
      301 gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt
      361 acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt
      421 gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc
      481 ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc
      541 tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt
      601 tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat
      661 ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag
      721 cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag
      781 tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc
      841 tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc
      901 ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag
      961 tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg
     1021 gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct
     1081 ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact
     1141 gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt
     1201 ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag
     1261 gtgtcgtgag gctagcatcg attgatcaac aagtttgtac aaaaaagcag gctggcgccg
     1321 gaaccaattc agtcgactgg atccggtacc gaattcaggc cggcctacca tgcggggccc
     1381 cggcacggcg gcgtcgcact cgcccctggg cctctgcgcc ctggtgcttg ctcttctggg
     1441 cgcgctgccc acggacaccc gggctcagcc atatcacggc gagaaaggca tctcggtacc
     1501 ggaccacggc ttctgccagc ccatctccat cccgttgtgc acggatatcg cctacaacca
     1561 gaccatcctg cccaacctgc tgggccacac gaaccaagag gacgcgggcc tcgaggtgca
     1621 ccagttctac cctctggtaa aggtgcagtg ttctcctgag ctacgcttct tcttatgctc
     1681 tatgtacgca cccgtgtgca ccgtgctcga ccaagccatt cctccgtgcc gttccttgtg
     1741 cgagcgcgcc cgacagggct gcgaggcgct catgaacaag ttcggcttcc agtggccaga
     1801 gcggttgcgc tgcgagaact tcccagtgca cggtgccggc gagatctgcg tggggcagaa
     1861 cacgtccgac ggctccgggg gcgcgggcgg cagtcccacc gcctacccta ctgctcccta
     1921 cctgccagac ccacctttca ctgcgatgtc cccctcagat ggcagaggcc gcttgtcttt
     1981 ccccttctcg tgtccgcgcc agctcaaggt gcccccctac ctgggctacc gcttcctagg
     2041 tgagcgtgac tgcggtgccc cgtgtgagcc gggccgtgct aacggcctca tgtactttaa
     2101 agaagaggag agacggttcg cccgcctctg ggtgggtgtg tggtcagtgc tgtgctgcgc
     2161 ctcgacgctc ttcacggtgc tcacctacct agtggacatg cgtcgcttca gctatccaga
     2221 gcgacccatc atcttcctgt cgggttgcta cttcatggtg gcagtggcgc acgtggcagg
     2281 cttcctgcta gaggaccgtg ccgtgtgcgt ggagcgcttc tcggacgatg gctaccgcac
     2341 ggtggcgcag ggcaccaaga aggagggctg caccatcctc ttcatggtgc tttacttctt
     2401 cggtatggcc agctccatct ggtgggtcat tctgtccctc acttggttcc tggcagctgg
     2461 catgaagtgg ggccacgagg ccatcgaggc caactcgcag tactttcatc tggccgcgtg
     2521 ggctgtgcca gcggtcaaga caatcaccat tttggccatg ggccaggtgg atggtgacct
     2581 actcagtgga gtgtgctacg tgggcctgtc tagtgtggat gcattgcggg gcttcgtgct
     2641 ggcgcccttg ttcgtctacc tcttcatcgg gacgtccttc ctgttggccg gctttgtgtc
     2701 tctctttcgc atccgcacca tcatgaagca cgacggcacc aagacagaga agctggagaa
     2761 gctgatggtg cgcatcggcg tcttcagcgt gctctacacg gtgccggcca ccatcgtgtt
     2821 ggcctgctac ttttatgagc aggccttccg agagcactgg gaacgcacct ggctcctgca
     2881 gacttgcaag agctacgctg tgccctgccc tccgggccac ttctctccca tgagccccga
     2941 ctttacagtc ttcatgatca agtacctgat gaccatgatc gtgggcatca ctacgggctt
     3001 ctggatctgg tcgggcaaga ccctgcagtc atggcgtcgc ttctaccaca gactcagcca
     3061 cagcagcaag ggggaaactg cggtatgagg cgcgcctcta gaactatagt gagtcgtatt
     3121 acgtagatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     3181 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     3241 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     3301 gggaggtgtg ggaggttttt taattcgcgg ccgcactcga gatatctaga cccagctttc
     3361 ttgtacaaag tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct
     3421 acgtagtaat gaactagtac cggttaagtc gacaatcaac gcgttaagtc gacaatcaac
     3481 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     3541 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     3601 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     3661 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     3721 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     3781 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     3841 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     3901 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     3961 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     4021 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat
     4081 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     4141 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
     4201 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     4261 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     4321 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag
     4381 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     4441 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     4501 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     4561 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     4621 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4681 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     4741 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     4801 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     4861 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     4921 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     4981 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5041 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5101 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5161 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5221 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5281 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5341 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     5401 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5461 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5521 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5581 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     5641 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5701 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5761 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     5821 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     5881 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5941 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6001 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6061 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6121 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6181 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6241 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6301 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6361 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6421 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6481 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6541 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6601 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6661 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     6721 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6781 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6841 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6901 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6961 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7021 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7081 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7141 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7201 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     7261 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     7321 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     7381 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     7441 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     7501 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     7561 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
     7621 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
     7681 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
     7741 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
     7801 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
     7861 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     7921 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     7981 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     8041 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     8101 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     8161 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     8221 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     8281 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     8341 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     8401 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     8461 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     8521 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     8581 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     8641 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     8701 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     8761 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     8821 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     8881 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     8941 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     9001 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     9061 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     9121 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     9181 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     9241 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     9301 cagacccacc tcccaacccc gaggggaccc atgcattgca tctcaattag tcagcaacca
     9361 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg cgtctcaatt
     9421 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     9481 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     9541 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     9601 gcaaaaagct ttctagaggt accaccggag cttaccatga ccgagtacaa gcccacggtg
     9661 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     9721 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     9781 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     9841 gacggcgccg ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     9901 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     9961 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
    10021 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
    10081 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
    10141 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
    10201 cgcacctggt gcatgacccg caagcccggt gccagttaac gaattctaga tcttgagaca
    10261 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
    10321 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
    10381 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac tttggcgccg
    10441 gctcga
//
