LOCUS       sequence-200086-       10410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for pop-in/pop-out replacement of Sec7 with 
            Sec7-mCherry2Bx6..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10410)
  AUTHORS   Day KJ, Casler JC, Glick BS
  TITLE     Budding Yeast Has a Minimal Endomembrane System.
  JOURNAL   Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi: 
            10.1016/j.devcel.2017.12.014. Epub 2018 Jan 8.
  PUBMED    29316441
REFERENCE   2  (bases 1 to 10410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.12.014"; journalName: "Dev Cell"; date: 
            "2018-01-8- 8"; volume: "44"; issue: "1"; pages: "56-72.e4"
COMMENT     Sequence Label: YIplac211-SEC7-mCherry2Bx6
FEATURES             Location/Qualifiers
     source          1..10410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7027..7043)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7027..7043)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7040..7062)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7051..7067
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7075..7081,7082..7099,7100..7105))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7120..7141
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7258..7275)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7429..8017)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7509..7528)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8188..8979,8980..9048))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8811..8830
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9049..9153)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(9320..10123)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(10124..10340)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     10383..10401
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ctattgttgt caaatgtgtt
      421 ttacagcttt tgatgataga gctgctcaac gaattattcg agaacgaaga ttttgcccac
      481 tgtatccctt acaaggaagc cattagaatt acaagattgt tggagaaatc gtacgaattt
      541 tctcgtgact ttaatgaaga ttatgggtta agaacaagac tagtagaggc tcgtgtagtt
      601 gataaaatac caaacctgtt gaaacaagaa acgagtgctg ctgcagttct ccttgacatt
      661 atgtttcagt tgtatctgaa cgatgatgag aagaaggctg atttaataac tcgtctgatc
      721 accatttgca tccaggtcgt agagggttat gtttctttgg atgatagaac catggaacgt
      781 agtatcaatg cctggcgttc agtgatagtt gaaatattac aaggttacta cgaatttgat
      841 gatgaagact ttagactata ctgtcctgct atgtatgctc tggtgattca aatattagat
      901 aagagcgttc caacggagtt gagacatgct ataaaacaat ttctaagcag agttggtgaa
      961 ttataccttt ctacggatcc aggaagttcg gggtccagcg gttcttcagg cgctagcatg
     1021 gatagcactg agagcggctc caccgagtcc gtcatcaagg agttcatgcg cttcaaggtg
     1081 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     1141 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     1201 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     1261 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     1321 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     1381 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     1441 cagaagaaga ctatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     1501 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     1561 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     1621 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     1681 gccgagggcc gccactccgg ctcccaggga tctagcggca gcagttctag catggatagc
     1741 actgagagcg gctccaccga gtccgtcatc aaggagttca tgcgcttcaa ggtgcacatg
     1801 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     1861 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     1921 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     1981 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     2041 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     2101 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     2161 aagactatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     2221 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     2281 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     2341 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     2401 ggccgccact ccggctccca gggatctagc ggcagcagtt ctagcatgga tagcactgag
     2461 agcggctcca ccgagtccgt catcaaggag ttcatgcgct tcaaggtgca catggagggc
     2521 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     2581 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     2641 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     2701 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     2761 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     2821 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagact
     2881 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     2941 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     3001 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     3061 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     3121 cactccggct cccagggatc tagcggcagc agttctagca tggatagcac tgagagcggc
     3181 tccaccgagt ccgtcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     3241 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     3301 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     3361 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     3421 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     3481 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     3541 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gactatgggc
     3601 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     3661 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     3721 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     3781 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     3841 ggctcccagg gatctagcgg cagcagttct agcatggata gcactgagag cggctccacc
     3901 gagtccgtca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     3961 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     4021 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     4081 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     4141 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     4201 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     4261 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagactat gggctgggag
     4321 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     4381 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     4441 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     4501 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccggctcc
     4561 cagggatcta gcggcagcag ttctagcatg gatagcactg agagcggctc caccgagtcc
     4621 gtcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     4681 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     4741 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     4801 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     4861 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     4921 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     4981 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ctatgggctg ggaggcctcc
     5041 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     5101 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     5161 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     5221 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccgg ctcccaggga
     5281 tctagcggca gcagttaagc ggccgggctt tgttgaatgg tccagcagat taaaatatgc
     5341 ttagttgtag aacacagaaa aaatgagaag gttgatatac ttttactcat attaatcctg
     5401 atttagtggc ggtgatcctt tttagattcg tttaattcat aaacaaggta catcatatag
     5461 caataacaaa tatgtaaact atgtagaaag acttatcaag tttagccatt ttggtcctca
     5521 ttcatgggca ttttatacta acttcatttt tgccgaaata agaaaggatg taatcttaac
     5581 ctaaaatatg taagcaatta agggcgcacg tagtcacttg cgaacaaata ggaaaagaaa
     5641 gaaacaagca cggatactga atgactgagt gtattactac acgatttatc tcaacgaatt
     5701 taagtgtgga ggacgatcgg ctgcacgtag gagtcaacgt ttttttactg tgttcatcac
     5761 aaaaccatta agctgagtca tccatattgc aaacaataag gtagcaccgc ttccatcaaa
     5821 acactagaac ataccgatgt tttcacggat ttattggaaa caaatagcag atgagggcgt
     5881 ttttacttgt aatgaaggcg aatgagaagg caaggcttac aggcttgttg tttatgccaa
     5941 gcatcaatac tcgctatgga ggagcgccat cagcgataac aacaaagttt gcaagtgctt
     6001 gcttggatat aaaaggcttg acgcttaaga taaatagggc catacttgct tgcttttact
     6061 atttttgttc ctcataagcc tgattgacta cgctattata aacccctaca ttatcctata
     6121 atcaacttca cttgtgtaac gttgaatgag ttcaactgag taatactcgg tcggaaatat
     6181 acgctccatc tggcaggaaa cataggctgc atttatatct tgcacaatta tgcccttcgt
     6241 aggtgtatgt tttcatgttt tccattatgt caatgtagat gtatctttgg acaggcaact
     6301 tttggatcaa taaagcacaa tttttgagca atttgagata tccagtattt gtacgggagt
     6361 cccaagattg tttagcctaa aaggttgagg acttggatta ttcttccatc aagcgcctcc
     6421 acccctcgca tcctatttct tttgaagcat aggggacgag actgtctgta atttatggcg
     6481 aagtatgaca agaaagattc aaactttgtt ttacgtatgc cccaataaat tctatggtag
     6541 cactactaga gacgtatttt ggttttgtaa taattttcaa actgctcgct atgatttgtt
     6601 acaagaaaca aacaaaagac acgaccatag ggttcatgtt ggtggttgcg aagccacatc
     6661 tttaaaacat tactgcaaat tcagcaggag tgagtagcaa aatcgacaaa aactagagta
     6721 cgcaacattc ccggtcgtaa atttcttatc gtggcgccag aacattcccg tccaatgatg
     6781 agaatgtctc taaaacaaat gcagtcacag agattttctg cgacttctcg agtgagaatg
     6841 ttgcttattg gtgctagtgg cttgagatct tcgtcaagca aattggaatg ccccagattc
     6901 tcaaacaagc acggcagaaa ctgaaatacg gccaaaatta tgaacccttt tgtgggatct
     6961 ttgtagaaat aggacctcag gatagtatga tgaagaatgt tggcatgcaa gcttggcgta
     7021 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7081 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7141 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7201 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     7261 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7321 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     7381 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     7441 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     7501 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     7561 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7621 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7681 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7741 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7801 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7861 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7921 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7981 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8041 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8101 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8161 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8221 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8281 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     8341 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     8401 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     8461 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     8521 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     8581 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8641 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8701 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8761 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8821 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8881 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8941 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9001 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9061 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9121 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9181 gctttttctt tccaattttt tttttttcgt cattatagaa atcattacga ccgagattcc
     9241 cgggtaataa ctgatataat taaattgaag ctctaatttg tgagtttagt atacatgcat
     9301 ttacttataa tacagttttt tagttttgct ggccgcatct tctcaaatat gcttcccagc
     9361 ctgcttttct gtaacgttca ccctctacct tagcatccct tccctttgca aatagtcctc
     9421 ttccaacaat aataatgtca gatcctgtag agaccacatc atccacggtt ctatactgtt
     9481 gacccaatgc gtctcccttg tcatctaaac ccacaccggg tgtcataatc aaccaatcgt
     9541 aaccttcatc tcttccaccc atgtctcttt gagcaataaa gccgataaca aaatctttgt
     9601 cgctcttcgc aatgtcaaca gtacccttag tatattctcc agtagctagg gagcccttgc
     9661 atgacaattc tgctaacatc aaaaggcctc taggttcctt tgttacttct tccgccgcct
     9721 gcttcaaacc gctaacaata cctgggccca ccacaccgtg tgcattcgta atgtctgccc
     9781 attctgctat tctgtataca cccgcagagt actgcaattt gactgtatta ccaatgtcag
     9841 caaattttct gtcttcgaag agtaaaaaat tgtacttggc ggataatgcc tttagcggct
     9901 taactgtgcc ctccatggaa aaatcagtca agatatccac atgtgttttt agtaaacaaa
     9961 ttttgggacc taatgcttca actaactcca gtaattcctt ggtggtacga acatccaatg
    10021 aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa tagcttggca gcaacaggac
    10081 taggatgagt agcagcacgt tccttatatg tagctttcga catgatttat cttcgtttcc
    10141 tgcaggtttt tgttctgtgc agttgggtta agaatactgg gcaatttcat gtttcttcaa
    10201 caccacatat gcgtatatat accaatctaa gtctgtgctc cttccttcgt tcttccttct
    10261 gctcggagat taccgaatca aaaaaatttc aaagaaaccg gaatcaaaaa aaagaacaaa
    10321 aaaaaaaaag atgaattgaa aagctctaag aaaccattat tatcatgaca ttaacctata
    10381 aaaataggcg tatcacgagg ccctttcgtc
//
