LOCUS       sequence-200087-       10447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-msCFPx6..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10447)
  AUTHORS   Day KJ, Casler JC, Glick BS
  TITLE     Budding Yeast Has a Minimal Endomembrane System.
  JOURNAL   Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi: 
            10.1016/j.devcel.2017.12.014. Epub 2018 Jan 8.
  PUBMED    29316441
REFERENCE   2  (bases 1 to 10447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.12.014"; journalName: "Dev Cell"; date: 
            "2018-01-8- 8"; volume: "44"; issue: "1"; pages: "56-72.e4"
COMMENT     Sequence Label: YIplac211-SEC7-msCFPx6
FEATURES             Location/Qualifiers
     source          1..10447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7064..7080)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7064..7080)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7077..7099)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7088..7104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7112..7118,7119..7136,7137..7142))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7157..7178
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7295..7312)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7466..8054)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7546..7565)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8225..9016,9017..9085))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8848..8867
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9086..9190)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(9357..10160)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(10161..10377)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     10420..10438
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ctattgttgt caaatgtgtt
      421 ttacagcttt tgatgataga gctgctcaac gaattattcg agaacgaaga ttttgcccac
      481 tgtatccctt acaaggaagc cattagaatt acaagattgt tggagaaatc gtacgaattt
      541 tctcgtgact ttaatgaaga ttatgggtta agaacaagac tagtagaggc tcgtgtagtt
      601 gataaaatac caaacctgtt gaaacaagaa acgagtgctg ctgcagttct ccttgacatt
      661 atgtttcagt tgtatctgaa cgatgatgag aagaaggctg atttaataac tcgtctgatc
      721 accatttgca tccaggtcgt agagggttat gtttctttgg atgatagaac catggaacgt
      781 agtatcaatg cctggcgttc agtgatagtt gaaatattac aaggttacta cgaatttgat
      841 gatgaagact ttagactata ctgtcctgct atgtatgctc tggtgattca aatattagat
      901 aagagcgttc caacggagtt gagacatgct ataaaacaat ttctaagcag agttggtgaa
      961 ttataccttt ctacggatcc aggaagttcg gggtccagcg gttcttcagg cgctagcatg
     1021 gatagcactg agagcctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1081 gtaaacggcc acaagttcag cgtgcgcggc gagggcgagg gcgatgccac caacggcaag
     1141 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1201 accaccctga cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     1261 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat caccttcaag
     1321 gacgacggca cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1381 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1441 gagtacaact tcatcagcga caacgtctat atcaccgccg acaagcagaa gaacggcatc
     1501 aaggccaact tcaagatccg ccacaacgtg gaggacggcg gcgtgcagct cgccgaccac
     1561 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1621 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1681 gagttcgtga ccgccgccgg gatcactcac ggcagctccg gctccagcgg gtctagcatg
     1741 gatagcactg agagcctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1801 gtaaacggcc acaagttcag cgtgcgcggc gagggcgagg gcgatgccac caacggcaag
     1861 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1921 accaccctga cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     1981 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat caccttcaag
     2041 gacgacggca cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     2101 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     2161 gagtacaact tcatcagcga caacgtctat atcaccgccg acaagcagaa gaacggcatc
     2221 aaggccaact tcaagatccg ccacaacgtg gaggacggcg gcgtgcagct cgccgaccac
     2281 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     2341 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     2401 gagttcgtga ccgccgccgg gatcactcac ggcagctccg gctccagcgg gtctagcatg
     2461 gatagcactg agagcctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     2521 gtaaacggcc acaagttcag cgtgcgcggc gagggcgagg gcgatgccac caacggcaag
     2581 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     2641 accaccctga cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     2701 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat caccttcaag
     2761 gacgacggca cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     2821 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     2881 gagtacaact tcatcagcga caacgtctat atcaccgccg acaagcagaa gaacggcatc
     2941 aaggccaact tcaagatccg ccacaacgtg gaggacggcg gcgtgcagct cgccgaccac
     3001 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     3061 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     3121 gagttcgtga ccgccgccgg gatcactcac ggcagctccg gctccagcgg gtctagcatg
     3181 gatagcactg agagcctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     3241 gtaaacggcc acaagttcag cgtgcgcggc gagggcgagg gcgatgccac caacggcaag
     3301 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     3361 accaccctga cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     3421 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat caccttcaag
     3481 gacgacggca cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     3541 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     3601 gagtacaact tcatcagcga caacgtctat atcaccgccg acaagcagaa gaacggcatc
     3661 aaggccaact tcaagatccg ccacaacgtg gaggacggcg gcgtgcagct cgccgaccac
     3721 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     3781 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     3841 gagttcgtga ccgccgccgg gatcactcac ggcagctccg gctccagcgg gtctagcatg
     3901 gatagcactg agagcctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     3961 gtaaacggcc acaagttcag cgtgcgcggc gagggcgagg gcgatgccac caacggcaag
     4021 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     4081 accaccctga cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     4141 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat caccttcaag
     4201 gacgacggca cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     4261 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     4321 gagtacaact tcatcagcga caacgtctat atcaccgccg acaagcagaa gaacggcatc
     4381 aaggccaact tcaagatccg ccacaacgtg gaggacggcg gcgtgcagct cgccgaccac
     4441 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     4501 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     4561 gagttcgtga ccgccgccgg gatcactcac ggcagctccg gctccagcgg gtctagcatg
     4621 gatagcactg agagcctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     4681 gtaaacggcc acaagttcag cgtgcgcggc gagggcgagg gcgatgccac caacggcaag
     4741 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     4801 accaccctga cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     4861 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat caccttcaag
     4921 gacgacggca cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     4981 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     5041 gagtacaact tcatcagcga caacgtctat atcaccgccg acaagcagaa gaacggcatc
     5101 aaggccaact tcaagatccg ccacaacgtg gaggacggcg gcgtgcagct cgccgaccac
     5161 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     5221 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     5281 gagttcgtga ccgccgccgg gatcactcac ggcagctccg gctccagcgg gtaaagcggc
     5341 cgggctttgt tgaatggtcc agcagattaa aatatgctta gttgtagaac acagaaaaaa
     5401 tgagaaggtt gatatacttt tactcatatt aatcctgatt tagtggcggt gatccttttt
     5461 agattcgttt aattcataaa caaggtacat catatagcaa taacaaatat gtaaactatg
     5521 tagaaagact tatcaagttt agccattttg gtcctcattc atgggcattt tatactaact
     5581 tcatttttgc cgaaataaga aaggatgtaa tcttaaccta aaatatgtaa gcaattaagg
     5641 gcgcacgtag tcacttgcga acaaatagga aaagaaagaa acaagcacgg atactgaatg
     5701 actgagtgta ttactacacg atttatctca acgaatttaa gtgtggagga cgatcggctg
     5761 cacgtaggag tcaacgtttt tttactgtgt tcatcacaaa accattaagc tgagtcatcc
     5821 atattgcaaa caataaggta gcaccgcttc catcaaaaca ctagaacata ccgatgtttt
     5881 cacggattta ttggaaacaa atagcagatg agggcgtttt tacttgtaat gaaggcgaat
     5941 gagaaggcaa ggcttacagg cttgttgttt atgccaagca tcaatactcg ctatggagga
     6001 gcgccatcag cgataacaac aaagtttgca agtgcttgct tggatataaa aggcttgacg
     6061 cttaagataa atagggccat acttgcttgc ttttactatt tttgttcctc ataagcctga
     6121 ttgactacgc tattataaac ccctacatta tcctataatc aacttcactt gtgtaacgtt
     6181 gaatgagttc aactgagtaa tactcggtcg gaaatatacg ctccatctgg caggaaacat
     6241 aggctgcatt tatatcttgc acaattatgc ccttcgtagg tgtatgtttt catgttttcc
     6301 attatgtcaa tgtagatgta tctttggaca ggcaactttt ggatcaataa agcacaattt
     6361 ttgagcaatt tgagatatcc agtatttgta cgggagtccc aagattgttt agcctaaaag
     6421 gttgaggact tggattattc ttccatcaag cgcctccacc cctcgcatcc tatttctttt
     6481 gaagcatagg ggacgagact gtctgtaatt tatggcgaag tatgacaaga aagattcaaa
     6541 ctttgtttta cgtatgcccc aataaattct atggtagcac tactagagac gtattttggt
     6601 tttgtaataa ttttcaaact gctcgctatg atttgttaca agaaacaaac aaaagacacg
     6661 accatagggt tcatgttggt ggttgcgaag ccacatcttt aaaacattac tgcaaattca
     6721 gcaggagtga gtagcaaaat cgacaaaaac tagagtacgc aacattcccg gtcgtaaatt
     6781 tcttatcgtg gcgccagaac attcccgtcc aatgatgaga atgtctctaa aacaaatgca
     6841 gtcacagaga ttttctgcga cttctcgagt gagaatgttg cttattggtg ctagtggctt
     6901 gagatcttcg tcaagcaaat tggaatgccc cagattctca aacaagcacg gcagaaactg
     6961 aaatacggcc aaaattatga acccttttgt gggatctttg tagaaatagg acctcaggat
     7021 agtatgatga agaatgttgg catgcaagct tggcgtaatc atggtcatag ctgtttcctg
     7081 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     7141 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     7201 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     7261 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     7321 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7381 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     7441 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7501 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7561 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7621 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7681 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7741 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7801 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7861 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     7921 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7981 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8041 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8101 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8161 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8221 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8281 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8341 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8401 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8461 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8521 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8581 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8641 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8701 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8761 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8821 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8881 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8941 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9001 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9061 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9121 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9181 gggttccgcg cacatttccc cgaaaagtgc cacctgagct ttttctttcc aatttttttt
     9241 ttttcgtcat tatagaaatc attacgaccg agattcccgg gtaataactg atataattaa
     9301 attgaagctc taatttgtga gtttagtata catgcattta cttataatac agttttttag
     9361 ttttgctggc cgcatcttct caaatatgct tcccagcctg cttttctgta acgttcaccc
     9421 tctaccttag catcccttcc ctttgcaaat agtcctcttc caacaataat aatgtcagat
     9481 cctgtagaga ccacatcatc cacggttcta tactgttgac ccaatgcgtc tcccttgtca
     9541 tctaaaccca caccgggtgt cataatcaac caatcgtaac cttcatctct tccacccatg
     9601 tctctttgag caataaagcc gataacaaaa tctttgtcgc tcttcgcaat gtcaacagta
     9661 cccttagtat attctccagt agctagggag cccttgcatg acaattctgc taacatcaaa
     9721 aggcctctag gttcctttgt tacttcttcc gccgcctgct tcaaaccgct aacaatacct
     9781 gggcccacca caccgtgtgc attcgtaatg tctgcccatt ctgctattct gtatacaccc
     9841 gcagagtact gcaatttgac tgtattacca atgtcagcaa attttctgtc ttcgaagagt
     9901 aaaaaattgt acttggcgga taatgccttt agcggcttaa ctgtgccctc catggaaaaa
     9961 tcagtcaaga tatccacatg tgtttttagt aaacaaattt tgggacctaa tgcttcaact
    10021 aactccagta attccttggt ggtacgaaca tccaatgaag cacacaagtt tgtttgcttt
    10081 tcgtgcatga tattaaatag cttggcagca acaggactag gatgagtagc agcacgttcc
    10141 ttatatgtag ctttcgacat gatttatctt cgtttcctgc aggtttttgt tctgtgcagt
    10201 tgggttaaga atactgggca atttcatgtt tcttcaacac cacatatgcg tatatatacc
    10261 aatctaagtc tgtgctcctt ccttcgttct tccttctgct cggagattac cgaatcaaaa
    10321 aaatttcaaa gaaaccggaa tcaaaaaaaa gaacaaaaaa aaaaaagatg aattgaaaag
    10381 ctctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
    10441 tttcgtc
//
