LOCUS       sequence-200113-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-tagged fragment (amino acids 1873-2025) of human Rev3
            protein with mutations abolishing Rev7 binding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Thanos Halazonetis
  TITLE     Expression plasmids for production of Rev7 and Champ1 proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDZ2FE_Rev3_1873-2025_4PA
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        17..68
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     19..39
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     113..132
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        113..131
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             160..183
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             202..225
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(762..779)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    768..992
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
       61 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
      121 ctcactatag ggagacccaa gctggctagc gcgacgatgg actacaagga cgacgatgac
      181 aagctcactg gttctaatgg tgactacaag gatgacgatg acaagggtgg taatggtaat
      241 ggtggtaagc ttggtcgtac tgctaacatc ctgaaagcac ttatgtcccc cgcaagtagg
      301 gaagaaatta tggcaacttt gttggatcat gacctgtctg agactattta ccaggaacca
      361 ttttgcagta atccttctga tgtaccagaa aagcccaggg agattggtgg acggctcctc
      421 atggtagaaa ctcgacttgc aaatgatctg gctgagtttg agggagactt ttccttggaa
      481 ggacttcgtc tttggaaaac agcattctca gcaatgactc agaatccaag gccagggtca
      541 ccccttcgca gtggccaagg agttgtcaat aaagggtcaa gtaatagccc taagatggtt
      601 gaagataaaa aaattgtgat tatggcttgc aaatgtgccg caagtcgaca actggttcaa
      661 gtgtggcttc aagccaaaga agaatacgaa cgttccaaga aactgcctaa aacctaagcg
      721 gccgctcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
      781 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
      841 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
      901 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
      961 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
//
