LOCUS       sequence-200114-        4776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-tagged full-length human POGZ protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Thanos Halazonetis
  TITLE     Expression plasmids for production of Rev7 and Champ1 proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCDZ2FE_Pogz_1-1410
FEATURES             Location/Qualifiers
     source          1..4776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        17..68
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     19..39
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     113..132
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        113..131
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             160..183
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             202..225
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(4518..4535)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4524..4748
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
       61 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
      121 ctcactatag ggagacccaa gctggctagc gcgacgatgg actacaagga cgacgatgac
      181 aagctcactg gttctaatgg tgactacaag gatgacgatg acaagggtgg taatggatcc
      241 atggcggaca ccgacctgtt catggaatgt gaggaggagg agttggagcc atggcagaaa
      301 atcagtgatg tcattgagga ctctgtagtt gaagattata attcagtgga taaaactacc
      361 acagtttctg tgagccagca gccagtctcg gctccagtgc ccatcgctgc ccatgcttct
      421 gttgctgggc acctctctac atccaccacc gttagtagca gcggggcaca gaacagcgac
      481 agtacaaaga agactcttgt cacactaatt gccaacaaca atgctggcaa tcctttggtc
      541 cagcaaggtg gacagccact catcctgacc cagaatccag ccccaggtct gggcacaatg
      601 gttactcaac cagtattgag gcctgttcag gtcatgcaga atgccaatca tgtgactagt
      661 tcccctgtgg cctcacaacc aatatttatc actacgcagg gatttcctgt aaggaatgtc
      721 cggcctgtac aaaatgcaat gaatcaggtt gggattgtgc tgaacgtaca gcaaggccaa
      781 acggttagac caattacact agttccagcc ccaggtaccc agtttgttaa gccgacagtt
      841 ggagttccac aagtgttctc ccagatgacc cctgtgaggc caggctccac aatgcctgtg
      901 aggcccacca ccaacacctt caccaccgtc atcccggcca ctcttaccat tcgaagcacc
      961 gtcccacagt cccagtccca gcagaccaag tccactccca gcacttctac cactcccact
     1021 gccacacagc caacctcact ggggcaacta gctgttcagt ctccaggcca gtcaaaccag
     1081 accacgaatc ccaagctagc tccctccttc ccctctccac ctgcagtgag cattgccagc
     1141 tttgtcactg tgaagcgacc tggtgttaca ggcgaaaata gcaatgaagt ggccaaattg
     1201 gtgaataccc ttaacaccat cccttccctg ggccagagtc ctgggccagt ggtggtgtcc
     1261 aacaacagct ctgctcatgg ctctcaaaga accagcggac ctgagtcttc aatgaaagtg
     1321 acctcttcca tcccagtatt tgacctccag gatggtggac ggaaaatatg tccacgatgt
     1381 aatgctcaat ttcgtgttac tgaagctttg agaggtcaca tgtgttactg ttgcccagaa
     1441 atggttgaat accagaagaa aggaaagtcc ctggattcag aacccagtgt cccatcagca
     1501 gcaaagcccc catcccctga gaaaacagct cctgttgctt ccacaccctc ttctacacct
     1561 attcctgctc tgtcaccgcc taccaaagta ccagaaccaa atgagaacgt gggcgatgcc
     1621 gtccagacca aactcattat gcttgtagat gacttctact atggacggga tggtggcaaa
     1681 gtagcccagc tcacaaattt ccctaaggtc gccacatctt tccgatgccc acattgtacc
     1741 aaaaggctaa aaaacaatat tcgattcatg aaccatatga aacaccacgt agaactcgat
     1801 cagcagaacg gtgaggtaga tggtcacact atctgccagc actgttaccg ccagttttcc
     1861 actcccttcc agcttcagtg ccacttggaa aatgttcata gtccctatga atctactacc
     1921 aagtgcaaga tctgtgaatg ggcgtttgaa agtgagccac tatttctcca gcatatgaag
     1981 gatactcata agcctggaga gatgccttat gtttgccagg tgtgtcaata tcgctcctca
     2041 ctctactctg aggtagatgt ccattttcgg atgatccatg aggatacccg gcatctgctc
     2101 tgcccttatt gcctgaaggt cttcaaaaat ggcaatgcat tccaacagca ttacatgagg
     2161 caccagaaga gaaatgttta tcactgcaac aaatgccggc tgcagtttct ctttgccaag
     2221 gacaaaattg aacacaagct tcaacaccat aaaaccttcc gtaaacccaa gcagctggag
     2281 ggcttgaaac caggcaccaa ggtgacaatc cgggcttccc gagggcagcc acgaactgtt
     2341 cctgtatcct ctaatgatac acctcccagc gccttgcagg aggcagcacc gctgacctcc
     2401 tcaatggacc ctctgcctgt cttcctttat ccccctgtcc agcgcagcat ccagaagaga
     2461 gctgttagga aaatgagtgt catgggccgg cagacatgcc tggagtgcag cttcgagatc
     2521 ccagacttcc ctaatcattt ccctacttac gtacactgct ctctgtgtcg ctatagcacc
     2581 tgctgttctc gagcttatgc caaccacatg atcaacaatc atgttccacg gaagagcccc
     2641 aagtatttgg ctttgtttaa aaattctgtg agtggaatca agctggcctg cacttcatgt
     2701 acctttgtta cctctgtggg cgatgctatg gccaagcatt tggtattcaa cccctctcac
     2761 agatccagca gcatcctgcc acggggactc acttggatag ctcactcaag gcatggccag
     2821 actcgtgacc gagtgcatga ccggaacgtg aagaatatgt accctcctcc ttccttcccc
     2881 actaacaaag ctgccactgt gaaatctgcg ggggccaccc cagctgagcc tgaagagcta
     2941 ctaactccct tagccccagc actcccatca ccagcctcaa ctgcaacccc accaccaacc
     3001 cccactcacc cgcaggcttt agcccttcca ccgctggcta cagagggagc cgaatgtctg
     3061 aatgttgatg atcaggatga agggagccca gtcacccaag aacctgagct agcatcaggt
     3121 ggtggtggta gtggtggagt tggcaaaaag gagcagctgt ctgtgaagaa gcttcgagta
     3181 gtactgtttg ctctatgctg caatacagaa caggcagctg aacacttccg aaatccccag
     3241 cgacgtattc gccgttggct tcgacgtttc caggcctccc agggggagaa tctagagggc
     3301 aaatatctga gctttgaggc agaagagaaa ctggctgagt gggtgctaac ccagcgcgaa
     3361 caacagctac ctgtaaatga ggagaccttg ttccagaagg ccaccaaaat aggacgttct
     3421 ttggaagggg ggtttaagat ctcctatgag tgggctgtgc gtttcatgct gcggcaccac
     3481 ctgactcccc atgcccggcg agctgtggcc cacaccctac ctaaggatgt agcagagaat
     3541 gcaggactct tcattgattt tgtacaacgg cagattcaca accaggactt acccttgtct
     3601 atgattgtgg ctattgatga gatctctttg ttcctggata cagaggtgct gagcagtgat
     3661 gatcgaaagg agaatgccct gcagacagtg ggcacagggg aaccttggtg tgatgtagtc
     3721 ctagccattc tggcagatgg cactgtcctt cccaccctgg ttttctacag agggcagatg
     3781 gatcagcctg ctaacatgcc agactccata ttgctagagg caaaggagag tggctacagt
     3841 gatgacgaga tcatggagct gtggtcaact cgagtgtggc agaagcacac agcttgccag
     3901 cgcagcaaag gcatgcttgt gatggactgt catcgcactc acttgtcaga agaggtactg
     3961 gctatgctta gtgcctctag cactttgcct gcagtggtcc cagcaggctg tagctccaaa
     4021 attcagccat tagatgtatg catcaaaaga actgtcaaga acttcctgca taaaaaatgg
     4081 aaggaacagg ctcgggaaat ggcagatact gcatgtgatt ctgatgtcct gcttcagctg
     4141 gtgcttgtct ggctgggtga agtgctaggt gtcattgggg actgtccaga gctagttcag
     4201 cgctccttcc tggtggctag tgttctgcct ggccccgatg gcaacattaa ctcacctaca
     4261 agaaatgctg acatgcagga ggagctaatt gcctccctag aggagcaact gaagctgagt
     4321 ggggaacatt ctgagtcttc cactccacga cccagatcat ctcctgaaga gacaattgag
     4381 cctgaaagtc ttcaccagct ctttgagggt gaaagtgaga ccgagtcttt ctatggcttt
     4441 gaagaagctg acctagatct gatggagatt tgagcggccg ctcgagtcta gagggcccgt
     4501 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     4561 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     4621 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     4681 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     4741 ctctatggct tctgaggcgg aaagaaccag ctgggg
//
