LOCUS       sequence-200121-        1518 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial Expression construct of N terminus of SIRT1: Exon1-Exon3 
            (deltaExon2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Deota S, Chattopadhyay T, Ramachandran D, Armstrong E, Camacho B, 
            Maniyadath B, Fulzele A, Gonzalez-de-Peredo A, Denu JM, 
            Kolthur-Seetharam U
  TITLE     Identification of a Tissue-Restricted Isoform of SIRT1 Defines a 
            Regulatory Domain that Encodes Specificity.
  JOURNAL   Cell Rep. 2017 Mar 28;18(13):3069-3077. doi: 
            10.1016/j.celrep.2017.03.012.
  PUBMED    28355560
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-03-28- 28"; volume: "18"; issue: "13"; pages: "3069-3077"
COMMENT     Sequence Label: pGEX-4T-3 SIRT1-NTerm Exon1-Exon3
FEATURES             Location/Qualifiers
     source          1..1518
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      646..655
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(710..727)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(754..776)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 gaaagggcat accggaacgc gcgaggcaga tcgtcagtca gtcacgatgc ggccgctcga
       61 gcccagctcc agtcagaact attatctttt tacactcctg cagtaacttc acagcatctt
      121 caattgtatt gatatctttt ctttttttcc gctttggtgg ttctgaaagg atattaataa
      181 caatctgcca cagcgtcata tcatccagct caggtggagg aattgtttct ggtaataaat
      241 ctttaagaat tgttcgagga tcggtgccaa tcatgagatg ttgctgaaca aaagtatatg
      301 gacctcggta gccgatcgct gccgccgccg ctgccgccgc cgcctcgtcc tcctcctcgc
      361 cctcgtcgtc gtcgaagtcg tcggccgccc tcggctcccg ccgcaggccg gacccattgt
      421 ctccgtcccc ggccacggcc gtcgccgggg cctcccgctc cgcgctcgcc gccgcccctg
      481 ccgcctcccg ccacagcgcc gccggggccg cggcgctcgc cgcctcacac cccgcggccg
      541 ccggcgccac tgctgcgctc ggctcgcccg ggctgcgccc gaggccaggc ccgtctcggc
      601 ggggcctctt gcggagcggc tcgtccgccg gctgcgacgc ggcctccatg gcggccgccg
      661 cggaagggga gccggcggcc tgaagggcga gcgccacctc gtccgccatg gatccacgcg
      721 gaaccagatc cgattttgga ggatggtcgc caccaccaaa cgtggcttgc cagccctgca
      781 aaggccatgc tatatacttg ctggatttca agtacttatc aatttgtggg atagcttcaa
      841 tacgtttttt aaaacaaact aattttggga acgcatccag gcacattggg gtccatgtat
      901 aaaacaacat caagagcgtc atacaacatg aagtcaggat gggttacatg atcaccattt
      961 aaatatgttt tatgacataa aacgatcttc gaacattttt cagcattttc aggtagcttg
     1021 gctaaaaaaa tcaactttga aaagtttcaa agtcttttac taatatgcaa tttctcgaaa
     1081 acaccgtatc taaaattcca aaaaccgctc ctttcaagca tttggaaatt cttgcccgct
     1141 ccttttggac aaaccacccc aacatggttg gggcttggca agctaataaa acgaaagaag
     1201 gggccataaa acgtggggtt aattttaacc ttcccccttc cttttaatta aggagaaaat
     1261 tggggaaaat tccccaaccc aaatttcaaa actttttggg tttcccccat tttacacccc
     1321 ttttttccgg ggccttaaac aaaaaggtcc cttctaattt ttttcttccc agaaatattc
     1381 tcaaaaggaa aaatcaaagg ggggtttggc caaaaggcgc cctttatatt tttcccaaaa
     1441 aaccccctgt taagtagggg gacaaagtaa aaaactggtt ctcctcgggg gagtagaaat
     1501 ttttttcctt ctccctaa
//
