LOCUS       sequence-200123-        1484 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial Expression construct of N terminus of SIRT1: 
            Exon1-Exon2-Exon3 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Deota S, Chattopadhyay T, Ramachandran D, Armstrong E, Camacho B, 
            Maniyadath B, Fulzele A, Gonzalez-de-Peredo A, Denu JM, 
            Kolthur-Seetharam U
  TITLE     Identification of a Tissue-Restricted Isoform of SIRT1 Defines a 
            Regulatory Domain that Encodes Specificity.
  JOURNAL   Cell Rep. 2017 Mar 28;18(13):3069-3077. doi: 
            10.1016/j.celrep.2017.03.012.
  PUBMED    28355560
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-03-28- 28"; volume: "18"; issue: "13"; pages: "3069-3077"
COMMENT     Sequence Label: pGEX-4T-3- SIRT1-NTerm Exon1-Exon2-Exon3
FEATURES             Location/Qualifiers
     source          1..1484
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      761..770
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(825..842)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(869..891)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 tgatgtcata acgaacgcgc gaggcagatc gtcagtcagt cacgatgcgg ccgctcgagc
       61 ccagctccag tcagaactat tatcttttta cactcctgca gtaacttcac agcatcttca
      121 attgtattga tatcttttct ttttttccgc tttggtggtt ctgaaaggat attaataaca
      181 atctgccaca gcgtcatatc atccagctca ggtggaggaa ttgtttctgg taataaatct
      241 ttaagaattg ttcgaggatc ggtgccaatc atgagatgtt gctgaacaaa agtatatgga
      301 cctatccgcg gccgcggagt ccagtcacta gagctggcgt gtgacgttct gtcatcgtca
      361 tcactttcac aggaatgaaa gccattagtg aggagtccat cggtcaacag gaggttgtct
      421 cggtagccga tcgctgccgc cgccgctgcc gccgccgcct cgtcctcctc ctcgccctcg
      481 tcgtcgtcga agtcgtcggc cgccctcggc tcccgccgca ggccggaccc attgtctccg
      541 tccccggcca cggccgtcgc cggggcctcc cgctccgcgc tcgccgccgc ccctgccgcc
      601 tcccgccaca gcgccgccgg ggccgcggcg ctcgccgcct cacaccccgc ggccgccggc
      661 gccactgctg cgctcggctc gcccgggctg cgcccgaggc caggcccgtc tcggcggggc
      721 ctcttgcgga gcggctcgtc cgccggctgc gacgcggcct ccatggcggc cgccgcggaa
      781 ggggagccgg cggcctgaag ggcgagcgcc acctcgtccg ccatggatcc acgcggaacc
      841 agatccgatt ttggaggatg gtcgccacca ccaaacgtgg cttgccagcc ctgcaaaggc
      901 catgctatat acttgctgga tttcaagtac ttatcaattt gtgggatagc ttcaatacgt
      961 tttttaaaac aaactaattt tggggaacgc atccaggcac attggggtcc atgtataaaa
     1021 caacatcaag agcgtcatac aacatggaag ccgggatggg ttacatggat caccatttaa
     1081 aatatggttt tatgaacata aaccgatctt tcgaacattt ttcagcattt tcagggtagc
     1141 ttggctaaga aaaatcaact tttgaaaagt ttccaagtcc tttactatat tgcaatttct
     1201 cgaaaacccc gtatctaaaa ttccaaaaac cgcttccttt caagccatgg gaaattcttt
     1261 ggcacccctc cttttgggga aaaccccccc caacatggtt tgggctttgg caagcctatt
     1321 aaaacggaag gaagggccca taaaactggg gtttaaattt aaactctccc ccttccaatt
     1381 taataaaggg gaaaatgggg ggaaaccctc ccaaacccca aattccaaac acttttttgt
     1441 ttttgcccct ttttatcaat cctcttttat tgcgttcttt ctca
//
