LOCUS       sequence-200163-       21879 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector containing Sr22 Schomburgk allele driven by Sr33 
            promoter, Sr33 promoter (undomesticated).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21879)
  AUTHORS   Md Hatta MA, Ghosh S, Athiyannan N, Richardson T, Steuernagel B, Yu 
            G, Rouse MN, Ayliffe M, Lagudah ES, Radhakrishnan GV, Periyannan SK,
            Wulff BBH
  TITLE     Extensive Genetic Variation at the Sr22 Wheat Stem Rust Resistance 
            Gene Locus in the Grasses Revealed Through Evolutionary Genomics and
            Functional Analyses.
  JOURNAL   Mol Plant Microbe Interact. 2020 Nov;33(11):1286-1298. doi: 
            10.1094/MPMI-01-20-0018-R. Epub 2020 Oct 1.
  PUBMED    32779520
REFERENCE   2  (bases 1 to 21879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-01-20-0018-R"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-11"; volume: "33"; issue: "11"; pages: 
            "1286-1298"
COMMENT     Sequence Label: pBW_0006
FEATURES             Location/Qualifiers
     source          1..21879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    416..440
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        1482..1827
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1770..1790
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    1857..1912
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             1914..2465
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      2488..3195
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(13037..13220)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(13073..13252)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    13376..13400
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      13888..14523
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(15198..15986)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      17083..17671
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     17572..17591
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     17825..17842
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(19031..20179)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     mobile_element  20804..21571
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     oriT            21631..21740
                     /note="incP origin of transfer"
ORIGIN
        1 gtcgacgatc gtcaacgttc acttctaaag aaatagcgcc actcagcttc ctcagcggct
       61 ttatccagcg atttcctatt atgtcggcat agttctcaag atcgacagcc tgtcacggtt
      121 aagcgagaaa tgaataagaa ggctgataat tcggatctct gcgagggaga tgatatttga
      181 tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc
      241 cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca
      301 aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg
      361 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca
      421 ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt
      481 ttttaatgta ctgaattcca atcccacaaa aatctgagct taacagcaca gttgctcctc
      541 tcagagcaga atcgggtatt caacaccctc atatcaacta ctacgttgtg tataacggtc
      601 cacatgccgg tatatacgat gactggggtt gtacaaaggc ggcaacaaac ggcgttcccg
      661 gagttgcaca caagaaattt gccactatta cagaggcaag agcagcagct gacgcgtaca
      721 caacaagtca gcaaacagac aggttgaact tcatccccaa aggagaagct caactcaagc
      781 ccaagagctt tgctaaggcc ctaacaagcc caccaaagca aaaagcccac tggctcacgc
      841 taggaaccaa aaggcccagc agtgatccag ccccaaaaga gatctccttt gccccggaga
      901 ttacaatgga cgatttcctc tatctttacg atctaggaag gaagttcgaa ggtgaaggtg
      961 acgacactat gttcaccact gataatgaga aggttagcct cttcaatttc agaaagaatg
     1021 ctgacccaca gatggttaga gaggcctacg cagcaggtct catcaagacg atctacccga
     1081 gtaacaatct ccaggagatc aaataccttc ccaagaaggt taaagatgca gtcaaaagat
     1141 tcaggactaa ttgcatcaag aacacagaga aagacatatt tctcaagatc agaagtacta
     1201 ttccagtatg gacgattcaa ggcttgcttc ataaaccaag gcaagtaata gagattggag
     1261 tctctaaaaa ggtagttcct actgaatcta aggccatgca tggagtctaa gattcaaatc
     1321 gaggatctaa cagaactcgc cgtgaagact ggcgaacagt tcatacagag tcttttacga
     1381 ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacactct ggtctactcc
     1441 aaaaatgtca aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg
     1501 ataatttcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg
     1561 acagtagaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggctatc
     1621 attcaagatc tctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
     1681 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgacatctcc
     1741 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
     1801 ggaagttcat ttcatttgga gaggacacgc tcgagtataa gagctctatt tttacaacaa
     1861 ttaccaacaa caacaaacaa caaacaacat tacaattact atttacaatt accatgagcc
     1921 cagaacgacg cccggccgac atccgccgtg ccaccgaggc ggacatgccg gcggtctgca
     1981 ccatcgtcaa ccactacatc gagacaagca cggtcaactt ccgtaccgag ccgcaggaac
     2041 cgcaggagtg gacggacgac ctcgtccgtc tgcgggagcg ctatccctgg ctcgtcgccg
     2101 aggtggacgg cgaggtcgcc ggcatcgcct acgcgggccc ctggaaggca cgcaacgcct
     2161 acgactggac ggccgagtca accgtgtacg tctccccccg ccaccagcgg acgggactgg
     2221 gctccacgct ctacacccac ctgctgaagt ccctggaggc acagggcttc aagagcgtgg
     2281 tcgctgtcat cgggctgccc aacgacccga gcgtgcgcat gcacgaggcg ctcggatatg
     2341 ccccccgcgg catgctgcgg gcggccggct tcaagcacgg gaactggcat gacgtgggtt
     2401 tctggcagct ggacttcagc ctgccggtac cgccccgtcc ggtcctgccc gtcaccgaga
     2461 tttgatgacc cgggggatcc tagagtcctg ctttaatgag atatgcgaga cgcctatgat
     2521 cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc
     2581 tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt tactatcgta
     2641 tttttatgaa taatattctc cgttcaattt actgattgta ccctactact tatatgtaca
     2701 atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc tatgatagag
     2761 cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa aaaagcggca
     2821 gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca aaagtgcccc
     2881 aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg aagggaactc
     2941 cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg ccgtccgctc
     3001 taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt aaccgacttg
     3061 ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg aagtgcaggt
     3121 caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt gcgacaacat
     3181 gtcgaggctc agcaggacct gcaggcatgc aagctctaga gcggccgctt taagagtgtt
     3241 gaggatgtgc ttacttaaat ttcaaaaggg ctatttaggg ataatatttg gtgaagcatg
     3301 gctggtggac tgaccatagg tacccactga ataaacatat cagcccaaag gcagagggta
     3361 tccctccctg taatacgtac tagtattcga ggagcgtgct aaagtagtat cctctcttga
     3421 tgctcctcgt ctccctccac tgagtactcc cttcctctcc tcattctcat ctcagtcctc
     3481 tcctcctccc tcgaataaga tagatccgcc cttgttgctg atctcatctc gcctcctatt
     3541 ggcctcgccc ctgccaactg actgctctat tggtgaccgt ggactttttg ggtatatata
     3601 tttcctgtcg atttgtttcc acttaaagta gcaatgcttg gatctaaaac ttattacttc
     3661 tcctgcttca gattctgatc catttgtgtt gattgaactc agggtgggat cgtggggctc
     3721 tgtatctatc taggatggat attccaagca atttactctc ttgagtgctg ttggtcttcc
     3781 tctgctcctc gtctccgctt cctctcagtc tcctccattg cgctgcctgg aacagatcga
     3841 tccgccttgt tgctgatctc atccatcctc cgtggagatt tcgggtggca gtttccagtg
     3901 actattcaga gcaccatact agtggcctgg taaaaatttc cctcccatct ttcttgctat
     3961 tctcttctta ttaatctgct gatttgcttg atggtaagtt cttacttatt cttccagatc
     4021 gagtgaagcg acatccgccg ccgtcttgtc ccctacgacg ccgctgtgtt ctgctctgtc
     4081 ctcctctgcc ggtgaagcat caacggtgac ggccatccgg tgttgacgcc gcggctgtgt
     4141 tctcctctgc cgtgaagtat caaaggtggg tttggatgga tatcacagtt tggattggca
     4201 actctgttct atagctcgtt gaaattaaag cttcacacag ttttgattgg attacagctc
     4261 cccatattca tctattttgc accatgcatc gaatccactg acctccacac gctcacggat
     4321 ctggcctctg gtgcccacac gctcacggat ccgacagagt gctcccttcc tatcatctca
     4381 gtcctcatct cctggtcgat ccggcttctt gtttggtgag ctcgtccaac ctccgagctc
     4441 gcccctgaca cctgtggagt ggaggtatat ttctttccag ctgaagtacg cttggatcta
     4501 aaaacttacg tctcctgctt cactgcttgc ttgggagtct gatcgatttg tgttggttga
     4561 atcatttggg gtggcagtgg agcagctagc aagaatcatg gagtttccaa tcattattca
     4621 gagcaccacg catggaatcc actgacctgg taactttgtc tcatctttct tgctactgaa
     4681 atcttccaca tccccacctt ccttgttcat ctgctgattt cccagattat acttccagat
     4741 tgagtgaagc gccgtcttgt cccctcacgg atctggcctc tggtgcccag ccgccgcttt
     4801 cgcttcgttg tccgacgagg ccaccgccgc tgtgttctgc tctgccgtga agcacaaaaa
     4861 ggtggggtct tcctctgctc tttctccact gacggcgatc cgaccttgac acccgtggcc
     4921 gtggcagtgg ggtcttcctc tgctctgttt ttttaccagt tattggatct aaaaagggat
     4981 ctaaaagtta ttacttctcc tgcttgagat tctgaccgat ttgtgttgat tgaatccagg
     5041 gtggggtcgt ggggctctgt atctatctag tacggagtac cactgtattc caagcaattt
     5101 actctcttga ctgctgttgg acttcctctg tccccgcttc cattgtgctg ccttgaacag
     5161 atccatccac cttgttgcta atctcatcca acctccgggt atgtttcttg tcgatttgtt
     5221 tccagtaaag tagcaatgct tgggtctaaa aagtcattgc ttctcctgct tgacgagtct
     5281 cattgatttg cccagtcatt attcagagca ccatgcatcc aaggcgctga cccggctata
     5341 agtttttatt acagggccgc cgtgttgtcc ccatcgagca aagcgtttaa tccactgacc
     5401 cgcttcgttg tccgcacagc gtgaccatcc gacgaagacg ccgccgttgt gttctgctct
     5461 cctctgaagc atcaaaaggt gggcttggtc cagatcccca cattcatcga tttggcttgc
     5521 caactcagtt ccaccagagc ttggattgtg ttacagatcc ctaaattcat cgatttgcag
     5581 gtttcagctg attgatcaag agagctctca tggcggaagt tctggtgagc gcatccacag
     5641 gggcaatggg ttccctcctg cggaagttgg gagctatgtt gacggatgaa tacaagctgc
     5701 tcaagaacgt ccgtggggac atcaagttcc tcaaggacga gctggaggtc atgtgcgcgt
     5761 tcctcctcaa gatgtcagat gtggaggagc ctgacgaacc aacaaagctg cgtgtgacgg
     5821 cggtgcggga gatgtcctat aagatagagg acaacatcga caagttcatg gtcctcgtgg
     5881 aacaggaaca cgggtcctcc tgttccgagg cggctcatgg cgtcgccaag ctcatggaca
     5941 agtgcaagaa cttgctgccg gacatcaagg cccgccgcag gatcgccaag gaggtcaaag
     6001 acatcaagaa agaaattaag gatgtcagcg acagattttc aaggtaccag atcttgttct
     6061 ccttacagta ctattagtac ctaccatcag atttgtgacc gatttgttgc cactcaaggt
     6121 acaagattga cgactcctcc tcctctatgc cggcaaaaga caaggtcgac cctcgacttc
     6181 gtgcagtcta caaagacgcc gcagagctcg tcggaattga tggaccaaag gatgaacttg
     6241 tcaagtggct gaatgagaaa gaaggccaat cactgaaatc tgtctctatt gttggatatg
     6301 gagggttggg caagaccacg ctcgccaacc agatccgggt caaccttgga gcgaccttcg
     6361 actgcggggc ttttgtctcg atttcacgca agcctgacat gaaggcgata ttaagatcta
     6421 tattatcaca aatcaccaag aaagacgatg cttgctctag attagatgat atacaactca
     6481 tcatcgacaa gattcgagaa ttcctccaag acacaaggta tgtatgtgag atctgctacg
     6541 aagtaacatt gcttaattag ctcatgcata atcggttgcc tagaattttg ctaatgaaga
     6601 ttgctttgtg tgatttactt tcgtcctcaa cttgtatgac tgcgcttaaa ctttcaagtc
     6661 attgtttctt gcaacttatg tcgatctgtt cactaattaa gatatagcta aaatgtgttt
     6721 gccgatttct gtcgccacac attttctaat ttgattgcca caagtttgtt aaacttgcgg
     6781 ctatgttctg tttattaaat aattgtgaca tggcgtcagt caaaacaata aagtacatgc
     6841 aattccctct cttttgtaca agataaatat ccgcacgcac tacatgactg caaatatatc
     6901 tatcttctaa ggacccaaag cacttgcatg tgcagaccat tgttaccgga tatgcagtac
     6961 attaggtttg tgattagcca ctttaagaac atagggtttc tagaggctat aaacctacgg
     7021 ttatcagtat tataatcttg aatggatatc aaacatccgt tgaagaatct atgcccataa
     7081 cacaaaaaga gaaaagggaa gaattaatca tagtcagatc ggaccagcct gactagcgta
     7141 acataatgac aacagtaggt tgttgcgtct ttcctggttt cgtagcaatt tgcatgacga
     7201 attgaggcgc agagccacca ctcaatatgt ggtcctacca atcatgagga aataacttgt
     7261 taccaaaatg acaaaagaaa ataggtagtc cccatcattg tttcaatatc tatcctcttg
     7321 cgaacatgct attggcagtg gcaaaacatt gtaccttgcc actggcaaca gctgccttta
     7381 ctcggcctga gcattcatat ttttttcctc ttggttgtta aatcttgcta tttttcgctt
     7441 gccttctatg gcaatctttt tttttcttat attgccacca aattgtatct aagagcaact
     7501 ccaacacgcc gacgcaattt ttgtccattt agatcggcca agcggacggg gtgtctggtt
     7561 ttgcctttgg gtggtgcgcc tacgcattag tccgcgtggg catttaaaaa acttaaaaag
     7621 cttaaaataa ttaaatataa ataaaaatgc atattaaagc ccgtcaaagt ccacataatt
     7681 aaacataata cattaatata ttaataaaaa acagccgccc cagcccgcag ccctaggcgt
     7741 cttcgtcgga ggagtgggtg aggtcgaaga atgccggcag ctcagcccac gggaaggcct
     7801 gctggtgggc agccagcacc gcctgctccg ctgtctgcgg cggcggcggc cctacctggc
     7861 accgcgcacg cctcctcctc gcgctcctgc cgctcctcct cctcatcgca cgcccaacga
     7921 agttttggcg ctcctccccc tcgggttccc gctgcatgcg gacctggccg tcgtaccgct
     7981 cctcctccag cgtgcggtcc ttccacgcca cgaggtaggc cgcctcctcc tccatcacgc
     8041 acagccaaat gggcggcttg ctcatccgct tgcagacgat gccctgccat tggtaaacgc
     8101 cggtgggcgc gggtggtgct agcgctggtg caggcggtga cgatgtggct ggtggtcccg
     8161 gcaggtagac actatagctg gcggccgata gctgcagcgt gtcatcgagg ccagaccact
     8221 aggagtcctc gcactaagac tcctcggtga cgtgatgggc cccctcctcc aatgccttga
     8281 ggaacgccgc ctcctcctca acctactatt tgaccaccgg tggcggcggt ggagggcagg
     8341 cttcgagctg gacggcgtcg cggaggcgca catggtgctc gatggtgaac cagacttctc
     8401 agttagggga gtctggcctc gatgggtgga gtcgcgacgc tgctctggcg tcaggagagc
     8461 acgccgggcc cgaatctcgt tggcgcgcgc acgctcggac tgtggcaccg ccggcactgg
     8521 aatgtggtgg gggtcgaggt gccagccgtg ggggaagttc acgtccggcc acggcagtga
     8581 gacgcggtac tcccggtggt actacgcgtg gtgcaccggg atgtactggt ggtcccgtgg
     8641 cgctgccgcc ctcccatccc gcagcgccga caccaatgcg aaccccttcg ctgccggcct
     8701 caccggccct tgcaacgagc cggcctcgtg gtcgtgcttg ccttgccctt acctttgcca
     8761 aagaaaccca tgacggctag ggttttctgt caccggcgag ggagcagatg tggactagag
     8821 tgggaggaga agtggatgag gcccgccccg ccgcaccctt gcaattaaaa agggcgtgat
     8881 ccggacgttt ctagtcactg ccatgcgggc ccgaggtagg tgggcgccat tgactacgtc
     8941 tgtggtggta gttggctggc agacacgcca gcccgaggcg cacaccgagc ggacgcgcaa
     9001 cacgtccgtc tcgtgttcgt gtgaacgcaa atcaggcaca aatttgggct ggaaatgcgt
     9061 ctacatggat gccgaacgga cgtgatttgg gtttgtgtcc gcacgttggg ccggcgtttt
     9121 tgtgcctttc gaccctaatg gacacacacg gaccaaatcc gttggcgtgt tagagttgct
     9181 tgtacctagt actactatgt gtggagttgt tatctttgag tttctccccc atctgtagct
     9241 gtgtattatt gtgagatgct ctcaggattt cacttatttt agataatgac aactttcttg
     9301 atgctagaca attacatcaa ggtgtattat atctaggatg cacacagatg tgagattgtt
     9361 tcaggatttt aggttacttg gtaggtgtta ctataaatat ctaatgagct ctaatgtgta
     9421 tgttcatgaa actgtgttgc aggtacttta tcataattga tgatatatgg gagttaggaa
     9481 catgggaaac tttgaaatgt gcatttgtca agaatacatt gggcagcaga ataattatca
     9541 caacacgtat agttgatgtt gccaaatctt gttctccttc tagtgaagat cttgtctatg
     9601 agatgaaacc actcagtgag gctgactcga agaaattgtt tttcaagagg atatttggct
     9661 gtgaagaaag ctgtcctgat agcttgaaag aagctgccaa tgatattttg aaaaaatgcc
     9721 gtggcctacc tctggccatc aatgccattt ctagcgtgtt ggtcacaaca agggaaacaa
     9781 aagaagagtg ggatcgggtg cgacattcaa tacgctcttc aaaggtcaaa agtgatataa
     9841 tagagaccat gaattacata ttgtctctta gttattttga tcttccccac cacctaagaa
     9901 gctgcttatt gtacctggct ctgtttcctg aagaccaatt gattggaagg aagcggttag
     9961 tacgcagatg gatttctgaa ggctttatcc atggagaaag tggacaagat ctcatggaat
    10021 taggagagga atattttcac cagcttgtga acagaagtct aatacagccc ggcaacatag
    10081 ggtatgacgg caaggcaatg tattgccgag tccatgacac catccttgat ttcctaattg
    10141 acaaatcctc tgaagagaac atgtgtactg tcctaaagaa acaatgcaag cctaatggca
    10201 tagttcgtcg gctctctcta atggggaatg aggatgaaga aatcgtggag caattggatc
    10261 tgtcacacgc tcggtcaatc actgcttttg gggatatcaa gctattgcct tcgcttggga
    10321 ggtcaaaatg cttgcgcgtg ctagacttgc aagactgcga tcagttggaa aatcatcata
    10381 tcaaggatat tgaaagactc tatcagctaa gatacttgga tatctcttct acaggaatca
    10441 cggagcttcc caggcaaatt ggggagttgc tgtatctaga gacgctagtt gcctatggat
    10501 tacgtgagct tcctgaaagc acaagtcggc tacaacgact ggcacgtttg tttgtttact
    10561 ctggttgtaa gcttccaggt gggttgggaa acttgataaa cttgcaagag cttgactgcg
    10621 ttgatgcctt gcatttgaag catgtggaag agctcggcaa actaacaaat ctgagaaagc
    10681 tgagtattaa attggacact ggtgggattg aaggcaacaa attagaagag tccaaggaaa
    10741 agctggtgtc ctctctctgt aaactggacg aatgcggcct cctttccctg agtattgatt
    10801 attatctgag agaaaaggat ggagaagagc cgttcctccc tgctttgggt tgtatccagg
    10861 aggtttttgt atatggacaa gatatcagcc ggattagcag atggctcgcg tcacttccca
    10921 acctacacag gttgcttttg gacgatccga agatagagca gcaggatatt gagatgattg
    10981 gattgatacc caatctgatc gatttgacac tgccgcctct atacaaaaca gacgacgctg
    11041 ggcggctcat catcaggcgt gaaggatttc aacagttgca gaagtttgag gcttacaata
    11101 ctcgtatggg ggttttaatg tttgaaccgg gggctatgcc gaggctcaag gagcttaaac
    11161 tccataactt catcgaaaag cccaaatctg ctgcggtcga tttcgacttt ggcatccaac
    11221 gcctctccag ccttgctcgc ctcactgtca gcttatcctg cggtggctgg acggtggcag
    11281 aagtagaggc tgcggaggat gctttcaaga gcatggccga ggcgaacccc aaccgtccaa
    11341 tactggagat gacaagatat aatacgcagc atatgttaca agacgagcaa ataggtatga
    11401 caggctcagg tactattaac tagtttgtac ttttgtcttg gtgagcagta gatcaaaaga
    11461 tgtgcatatg cttcaatctt ttttccggta atctatttca gctacaacgc cggccgtcaa
    11521 gtcctagctg acgttgcttc aggtgtgctc tcaatctata catatttact catcatatta
    11581 ttctccacct ttttttccat ctctagagct cagcttgtca ttgcattgtt caattgtgct
    11641 tctctagcag ctacggaaat tctgaacagg caaaggtaat tttacagaag tttctttggc
    11701 ataagtatct gagaaaaatg cacttggcac aatttacctt ttcgcagtag aaaagagtca
    11761 gtggatcata ccttcttaat tgttcgattg ctcgtaatac ttgggggcac tctatgattg
    11821 ctgtgtgctt ttagttttga tatagttcct gttagcatgg acagactgtg tgattggttc
    11881 atgaatttca gggtggttct aatgaggaaa tgatgcttgc ttcagaaata gtttccattt
    11941 gtacctaagc tgtgtagttg gtaaagttgc attttggatg ttctgttggt atagcttaca
    12001 gaagaaagac ttgcgttgtg caggtaccag gggcgcaggt tactgctagt ggcgtctgag
    12061 atgtttcata aacgttgggt gggcaccggt catgcaaaaa attgaaggat aaaaaggttc
    12121 cgagaggttg tgtgatgtgc tccttttaaa tggcagtcgc ttgcataaag agatgttgct
    12181 tggtttttta gtaaagcgtt gtagtaagtt gctctgtttc ggctgagagc catcgttgtt
    12241 gctgtctgtt acatcgaatg gctggctcat gtcatgttga tgtttttttt atctgaaccg
    12301 gacaaacctg agggtgtagt atcatgtttg taatgactga aattctgggc tcacccgagc
    12361 tccttaattc gtaaacaact gcgactcatt gttggattcc ttacttcaga aaatatttac
    12421 ttgctctggc tcatacagct ctgcatgttt tgtagtggtt tgtcgctgtt taggtgctgc
    12481 ttcagactcc tggtaccttt ggccgctgct gctactgcat gagctttgta tggcattgtt
    12541 agtatagttt ggtgtcattg ggttttcgcc ccatggtgag tgtccattgc gcgcactcgc
    12601 agcggtttcc tggcgataag ttgaacttga tctttctctt tcccacctcc taaacgtcta
    12661 ttcagacttg tgtatatttg cattacgggg attacccgag ttcaaaaata aatattattt
    12721 actagttctg ccaatgtgtt tccgttacat acaaataaaa gggtgcttcc agctacaagt
    12781 atatttttga gaagggccat tgcaaaacga aatgaagccc aaattctacc aacatgtttc
    12841 tgtcacatat acattaaaaa aacggtgtaa atgtcccgtc aaaaaagggt gtatgtagga
    12901 aaaggagttc gacgccgcca gctccctcct aacgctcaga ttgtcttcgc ggccgccacc
    12961 gcggctgagt ggctccttca agcttgcgga tccgggcgga aataggtaaa gaagttgcgg
    13021 ataaggtaat tgccattgca gattatttgg attgagagtg aatatgagac tctaattgga
    13081 taccgagggg aatttatgga acgtcagtgg agcatttttg acaagaaata tttgctagct
    13141 gatagtgacc ttaggcgact tttgaacgcg caataatggt ttctgacgta tgtgcttagc
    13201 tcattaaact ccagaaaccc gcggctgagt ggctccttca acgttgcggt tctgtcagtt
    13261 ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt cataacgtga ctcccttaat
    13321 tctccgctca tgatcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca
    13381 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta
    13441 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt
    13501 cccctcggga gtgcttggca ttccgtgcga taatgacttc tgttcaacca cccaaacgtc
    13561 ggaaagcctg acgacggagc agcattccaa aaagatccct tggctcgtct gggtcggcta
    13621 gaaggtcgag tgggctgctg tggcttgatc cctcaacgcg gtcgcggacg tagcgcagcg
    13681 ccgaaaaatc ctcgatcgca aatccgacgc tgtcgaaaag cgtgatctgc ttgtcgctct
    13741 ttcggccgac gtcctggcca gtcatcacgc gccaaagttc cgtcacagga tgatctggcg
    13801 cgagttgctg gatctcgcct tcaatccggg tctgtggcgg gaactccacg aaaatatccg
    13861 aacgcagcaa gatcgtcgac cctttccgac gctcaccggg ctggttgccc tcgccgctgg
    13921 gctggcggcc gtctatggcc ctgcaaacgc gccagaaacg ccgtcgaagc cgtgtgcgag
    13981 acaccgcggc cggccgccgg cgttgtggat acctcgcgga aaacttggcc ctcactgaca
    14041 gatgaggggc ggacgttgac acttgagggg ccgactcacc cggcgcggcg ttgacagatg
    14101 aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc agcctcgcaa atcggcgaaa
    14161 acgcctgatt ttacgcgagt ttcccacaga tgatgtggac aagcctgggg ataagtgccc
    14221 tgcggtattg acacttgagg ggcgcgacta ctgacagatg aggggcgcga tccttgacac
    14281 ttgaggggca gagtgctgac agatgagggg cgcacctatt gacatttgag gggctgtcca
    14341 caggcagaaa atccagcatt tgcaagggtt tccgcccgtt tttcggccac cgctaacctg
    14401 tcttttaacc tgcttttaaa ccaatattta taaaccttgt ttttaaccag ggctgcgccc
    14461 tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc ttctcgaacc ctcccggccc
    14521 gctaacgcgg gcctcccatc cccccagggg ctgcgcccct cggccgcgaa cggcctcacc
    14581 ccaaaaatgg cagccaagct cctaacattt tattagagag caggctagtt gcttagatac
    14641 atgatcttca ggccgttatc tgtcagggca agcgaaaatt ggccatttat gacgaccaat
    14701 gccccgcaga agctcccatc tttgccgcca tagacgccgc gccccccttt tggggtgtag
    14761 aacatccttt tgccagatgt ggaaaagaag ttcgttgtcc cattgttggc aatgacgtag
    14821 tagccggcga aagtgcgaga cccatttgcg ctatatataa gcctacgatt tccgttgcga
    14881 ctattgtcgt aattggatga actattatcg tagttgctct cagagttgtc gtaatttgat
    14941 ggactattgt cgtaattgct tatggagttg tcgtagttgc ttggagaaat gtcgtagttg
    15001 gatggggagt agtcataggg aagacgagct tcatccacta aaacaattgg caggtcagca
    15061 agtgcctgcc ccgatgccat cgcaagtacg aggcttagaa ccaccttcaa cagatcgcgc
    15121 atagtcttcc ccagctctct aacgcttgag ttaagccgcg ccgcgaagcg gcgtcggctt
    15181 gaacgaattg ttagacatta tttgccgact accttggtga tctcgccttt cacgtagtga
    15241 acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttgtcc aagataagcc
    15301 tgcctagctt caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg
    15361 gcagcgacat ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac
    15421 gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag
    15481 gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc
    15541 gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca
    15601 atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca
    15661 aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg
    15721 gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg
    15781 tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa
    15841 tcaatatcac tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc
    15901 agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat
    15961 acttcggcga tcaccgcttc cctcatgatg tttaactcct gaattaagcc gcgccgcgaa
    16021 gcggtgtcgg cttgaatgaa ttgttaggcg tcatcctgtg ctcccgagaa ccagtaccag
    16081 tacatcgctg tttcgttcga gacttgaggt ctagttttat acgtgaacag gtcaatgccg
    16141 ccgagagtaa agccacattt tgcgtacaaa ttgcaggcag gtacattgtt cgtttgtgtc
    16201 tctaatcgta tgccaaggag ctgtctgctt agtgcccact ttttcgcaaa ttcgatgaga
    16261 ctgtgcgcga ctcctttgcc tcggtgcgtg tgcgacacaa caatgtgttc gatagaggct
    16321 agatcgttcc atgttgagtt gagttcaatc ttcccgacaa gctcttggtc gatgaatgcg
    16381 ccatagcaag cagagtcttc atcagagtca tcatccgaga tgtaatcctt ccggtagggg
    16441 ctcacacttc tggtagatag ttcaaagcct tggtcggata ggtgcacatc gaacacttca
    16501 cgaacaatga aatggttctc agcatccaat gtttccgcca cctgctcagg gatcaccgaa
    16561 atcttcatat gacgcctaac gcctggcaca gcggatcgca aacctggcgc ggcttttggc
    16621 acaaaaggcg tgacaggttt gcgaatccgt tgctgccact tgtttaatag actggatgga
    16681 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
    16741 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
    16801 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
    16861 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
    16921 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
    16981 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
    17041 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgatatc ctttttttct
    17101 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
    17161 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
    17221 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
    17281 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
    17341 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
    17401 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
    17461 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
    17521 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
    17581 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    17641 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
    17701 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
    17761 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
    17821 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
    17881 gcatctgtgc ggtatttcac accgcatatg aagatcggcg gcaatagctt cttagcgcca
    17941 tcccggctga gaaagcccag taaggaaaca actgtaggtt cgagtcgcga gatcccccgg
    18001 aaccaaagga agtaggttaa acccgctccg atcaggccga gccacgccag gccgagaaca
    18061 ttggttcctg taggcatcgg gattggcgga tcaaacacta aagctactgg aacgagcaga
    18121 agtcctccgg ccgccagttg ccaggcggta aaggtgagca gaggcacggg aggttgccac
    18181 ttgcgggtca gcacggttcc gaacgccatg gaaaccgccc ccgccaggcc cgctgcgacg
    18241 ccgacaggat ctagcgctgc gtttggtgtc aacaccaaca gcgccacgcc cgcagttccg
    18301 caaatagccc ccaggaccgc catcaatcgt atcgggctac ctagcagagc ggcagagatg
    18361 aacacgacca tcagcggctg cacagcgcct accgtcgccg cgaccccgcc cggcaggcgg
    18421 tagaccgaaa taaacaacaa gctccagaat agcgaaatat taagtgcgcc gaggatgaag
    18481 atgcgcatcc accagattcc cgttggaatc tgtcggacga tcatcacgag caataaaccc
    18541 gccggcaacg cccgcagcag cataccggcg acccctcggc ctcgctgttc gggctccacg
    18601 aaaacgccgg acagatgcgc cttgtgagcg tccttggggc cgtcctcctg tttgaagacc
    18661 gacagcccaa tgatctcgcc gtcgatgtag gcgccgaatg ccacggcatc tcgcaaccgt
    18721 tcagcgaacg cctccatggg ctttttctcc tcgtgctcgt aaacggaccc gaacatctct
    18781 ggagctttct tcagggccga caatcggatc tcgcggaaat cctgcacgtc ggccgctcca
    18841 agccgtcgaa tctgagcctt aatcacaatt gtcaatttta atcctctgtt tatcggcagt
    18901 tcgtagagcg cgccgtgcgt cccgagcgat actgagcgaa gcaagtgcgt cgagcagtgc
    18961 ccgcttgttc ctgaaatgcc agtaaagcgc tggctgctga acccccagcc ggaactgacc
    19021 ccacaaggcc ctagcgtttg caatgcacca ggtcatcatt gacccaggcg tgttccacca
    19081 ggccgctgcc tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc
    19141 gggtggaatc cgatccgcac atgaggcgga aggtttccag cttgagcggg tacggctccc
    19201 ggtgcgagct gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg cggtacttct
    19261 cccatatgaa tttcgtgtag tggtcgccag caaacagcac gacgatttcc tcgtcgatca
    19321 ggacctggca acgggacgtt ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg
    19381 acaccgattc caggtgccca acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc
    19441 gcgacaggca ttcctcggcc ttcgtgtaat accggccatt gatcgaccag cccaggtcct
    19501 ggcaaagctc gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact
    19561 ccaacacctg ctgccacacc agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg
    19621 tgatcttcac gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga
    19681 ttttcttgtt gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc atcgctcgca
    19741 tcgtgtccgg ccacggcgca atatcgaaca aggaaagctg catttccttg atctgctgct
    19801 tcgtgtgttt cagcaacgcg gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc
    19861 cggcggtttt tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg
    19921 ccaaacctgc cgcctcctgt tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg
    19981 gcagggcagg gggagccagt tgcacgctgt cgcgctcgat cttggccgta gcttgctgga
    20041 ccatcgagcc gacggactgg aaggtttcgc ggggcgcacg catgacggtg cggcttgcga
    20101 tggtttcggc atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc
    20161 ggtcaaacgt ccgattcatt caccctcctt gcgggattgc cccgactcac gccggggcaa
    20221 tgtgccctta ttcctgattt gacccgcctg gtgccttggt gtccagataa tccaccttat
    20281 cggcaatgaa gtcggtcccg tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa
    20341 tcttgccctg cacgaatacc agctccgcga agtcgctctt cttgatggag cgcatgggga
    20401 cgtgcttggc aatcacgcgc accccccggc cgttttagcg gctaaaaaag tcatggctct
    20461 gccctcgggc ggaccacgcc catcatgacc ttgccaagct cgtcctgctt ctcttcgatc
    20521 ttcgccagca gggcgaggat cgtggcatca ccgaaccgcg ccgtgcgcgg gtcgtcggtg
    20581 agccagagtt tcagcaggcc gcccaggcgg cccaggtcgc cattgatgcg ggccagctcg
    20641 cggacgtgct catagtccac gacgcccgtg attttgtagc cctggccgac ggccagcagg
    20701 taggccgaca ggctcatgcc ggccgccgcc gccttttcct caatcgctct tcgttcgtct
    20761 ggaaggcagt acaccttgat aggtgggctg cccttcctgg ttgggtaatg actccaactt
    20821 attgatagtg ttttatgttc agataatgcc cgatgacttt gtcatgcagc tccaccgatt
    20881 ttgagaacga cagcgacttc cgtcccagcc gtgccaggtg ctgcctcaga ttcaggttat
    20941 gccgctcaat tcgctgcgta tatcgcttgc tgattacgtg cagctttccc ttcaggcggg
    21001 attcatacag cggccagcca tccgtcatcc atatcaccac gtcaaagggt gacagcaggc
    21061 tcataagacg ccccagcgtc gccatagtgc gttcaccgaa tacgtgcgca acaaccgtct
    21121 tccggagact gtcatacgcg taaaacagcc agcgctggcg cgatttagcc ccgacatagc
    21181 cccactgttc gtccatttcc gcgcagacga tgacgtcact gcccggctgt atgcgcgagg
    21241 ttaccgactg cggcctgagt tttttaagtg acgtaaaatc gtgttgaggc caacgcccat
    21301 aatgcgggct gttgcccggc atccaacgcc attcatggcc atatcaatga ttttctggtg
    21361 cgtaccgggt tgagaagcgg tgtaagtgaa ctgcagttgc catgttttac ggcagtgaga
    21421 gcagagatag cgctgatgtc cggcggtgct tttgccgtta cgcaccaccc cgtcagtagc
    21481 tgaacaggag ggacagctga tagaaacaga agccactgga gcacctcaaa aacaccatca
    21541 tacactaaat cagtaagttg gcagcatcac ccctggttgg cttggtttca tcagccatcc
    21601 gcttgccctc atctgttacg ccggcggtag ccggccagcc tcgcagagca ggattcccgt
    21661 tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc
    21721 ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa
    21781 ccctttggca aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta
    21841 taatgacccc gaagcagggt tatgcagcgg aaaagatcc
//
