LOCUS       sequence-200167-        7162 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Swapped helices.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7162)
  AUTHORS   Arakel EC, Richter KP, Clancy A, Schwappach B
  TITLE     delta-COP contains a helix C-terminal to its longin domain key to 
            COPI dynamics and function.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):6916-21. doi: 
            10.1073/pnas.1603544113. Epub 2016 Jun 13.
  PUBMED    27298352
REFERENCE   2  (bases 1 to 7162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1603544113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-06-21- 21"; volume: "113"; issue: "25"; pages: "6916-21"
COMMENT     Sequence Label: P415 delta-CLD+Ret2 2-alpha
FEATURES             Location/Qualifiers
     source          1..7162
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(199..218)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     318..340
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(378..396)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    438..941
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        978..1082
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1083..1151,1152..1943)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1301..1320)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2114..2702
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2603..2622
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2856..2873
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2990..3011
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3026..3031,3032..3049,3050..3056)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3064..3080
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3069..3091
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3088..3104
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3088..3104
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3123..3143
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3125..3143
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3194..3543
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
     primer_bind     3544..3560
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3544..3560
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             4093..4110
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4137..4153)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4138..4154)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      4156..4403
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4172..4190)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(4421..4440)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4422..4440)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4450..4467)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4450..4466)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4459..4481)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4607..5062
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4694..4713)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4904..4925
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5363..5772
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             5773..6867
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 ttcctaattt atatattgga ggattttctc taaaaaaaaa aaaatacaac aaataaaaaa
       61 cactcaatga cctgaccatt tgatggagtt taagtcaata ccttcttgaa ccatttccca
      121 taatggtgaa agttccctca agaattttac tctgtcagaa acggccttaa cgacgtagtc
      181 gatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
      241 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
      301 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
      361 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
      421 aatggtttct taggacggat cgcttgcctg taacttacac gcgcctcgta tcttttaatg
      481 atggaataat ttgggaattt actctgtgtt tatttatttt tatgttttgt atttggattt
      541 tagaaagtaa ataaagaagg tagaagagtt acggaatgaa gaaaaaaaaa taaacaaagg
      601 tttaaaaaat ttcaacaaaa agcgtacttt acatatatat ttattagaca agaaaagcag
      661 attaaataga tatacattcg attaacgata agtaaaatgt aaaatcacag gattttcgtg
      721 tgtggtcttc tacacagaca agatgaaaca attcggcatt aatacctgag agcaggaaga
      781 gcaagataaa aggtagtatt tgttggcgat ccccctagag tcttttacat cttcggaaaa
      841 caaaaactat tttttcttta atttcttttt ttactttcta tttttaattt atatatttat
      901 attaaaaaat ttaaattata attattttta tagcacgtga tgaaaaggac ccaggtggca
      961 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     1021 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     1081 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     1141 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     1201 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     1261 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     1321 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     1381 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     1441 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     1501 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1561 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1621 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     1681 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     1741 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     1801 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     1861 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     1921 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     1981 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     2041 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2101 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2161 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2221 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     2281 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2341 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2401 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2461 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2521 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2581 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2641 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2701 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2761 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     2821 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     2881 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     2941 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     3001 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     3061 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     3121 gcgcaattaa ccctcactaa agggaacaaa agctggagct ccggatgcaa gggttcgaat
     3181 cccttagctc tcattatttt ttgctttttc tcttgaggtc acatgatcgc aaaatggcaa
     3241 atggcacgtg aagctgtcga tattggggaa ctgtggtggt tggcaaatga ctaattaagt
     3301 tagtcaaggc gccatcctca tgaaaactgt gtaacataat aaccgaagtg tcgaaaaggt
     3361 ggcaccttgt ccaattgaac acgctcgatg aaaaaaataa gatatatata aggttaagta
     3421 aagcgtctgt tagaaaggaa gtttttcctt tttcttgctc tcttgtcttt tcatctacta
     3481 tttccttcgt gtaatacagg gtcgtcagat acatagatac aattctatta cccccatcca
     3541 tactctagaa ctagtggatc caccatggtg ctgttggcag cagcagtctg tacaaaagca
     3601 ggaaaagcta ttgtttctcg acagtttgta gagatgactc gaactcggat tgagggcttg
     3661 ttagcagctt ttccaaagct catgaacact ggaaaacaac atacatttgt ggaaacagag
     3721 agtgtcagat atgtttacca gcctatggag aaattgtaca tggtactgat caccaccaaa
     3781 aacagcaaca tcttagaaga tctggagacc ctaaggctct tctcaagagt gattcctgaa
     3841 tattgccgag ccttagaaga gaatgaaata tctgagcact gttttgattt gatttttgca
     3901 tttgacgaaa ttgttgcctt gggatacaaa gagaacttgt cctttactca agtgcaaaca
     3961 tacttgtcta tggagtcgca cgaagaaaga atccaggaaa ttattgagcg taataaagag
     4021 atcgaagcta ctgaggagag gaagcgccgt gctaaggaaa ttgctagaaa agaacatgaa
     4081 aggaaacacg ggcatcacca tcaccatcac tgaccggaat tcgatatcaa gcttatcgat
     4141 accgtcgacc tcgagtcatg taattagtta tgtcacgctt acattcacgc cctcccccca
     4201 catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt
     4261 tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc
     4321 tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg
     4381 gacgctcgaa ggctttaatt tgcggccggt acccaattcg ccctatagtg agtcgtatta
     4441 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     4501 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     4561 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggacgc gccctgtagc
     4621 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     4681 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     4741 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     4801 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     4861 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     4921 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     4981 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     5041 aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta
     5101 tttcacaccg catatcgacc ggtcgaggag aacttctagt atatctacat acctaatatt
     5161 attgccttat taaaaatgga atcccaacaa ttacatcaaa atccacattc tcttcaaaat
     5221 caattgtcct gtacttcctt gttcatgtgt gttcaaaaac gttatattta taggataatt
     5281 atactctatt tctcaacaag taattggttg tttggccgag cggtctaagg cgcctgattc
     5341 aagaaatatc ttgaccgcag ttaactgtgg gaatactcag gtatcgtaag atgcaagagt
     5401 tcgaatctct tagcaaccat tatttttttc ctcaacataa cgagaacaca caggggcgct
     5461 atcgcacaga atcaaattcg atgactggaa attttttgtt aatttcagag gtcgcctgac
     5521 gcatatacct ttttcaactg aaaaattggg agaaaaagga aaggtgagag cgccggaacc
     5581 ggcttttcat atagaataga gaagcgttca tgactaaatg cttgcatcac aatacttgaa
     5641 gttgacaata ttatttaagg acctattgtt ttttccaata ggtggttagc aatcgtctta
     5701 ctttctaact tttcttacct tttacatttc agcnanatat atatatatat atttcaagga
     5761 tataccattc taatgtctgc ccctaagaag atcgtcgttt tgccaggtga ccacgttggt
     5821 caagaaatca cagccgaagc cattaaggtt cttaaagcta tttctgatgt tcgttccaat
     5881 gtcaagttcg atttcgaaaa tcatttaatt ggtggtgctg ctatcgatgc tacaggtgtt
     5941 ccacttccag atgaggcgct ggaagcctcc aagaaggctg atgccgtttt gttaggtgct
     6001 gtgggtggtc ctaaatgggg taccggtagt gttagacctg aacaaggttt actaaaaatc
     6061 cgtaaagaac ttcaattgta cgccaactta agaccatgta actttgcatc cgactctctt
     6121 ttagacttat ctccaatcaa gccacaattt gctaaaggta ctgacttcgt tgttgtcaga
     6181 gaattagtgg gaggtattta ctttggtaag agaaaggaag acgatggtga tggtgtcgct
     6241 tgggatagtg aacaatacac cgttccagaa gtgcaaagaa tcacaagaat ggccgctttc
     6301 atggccctac aacatgagcc accattgcct atttggtcct tggataaagc taatgttttg
     6361 gcctcttcaa gattatggag aaaaactgtg gaggaaacca tcaagaacga attccctaca
     6421 ttgaaggttc aacatcaatt gattgattct gccgccatga tcctagttaa gaacccaacc
     6481 cacctaaatg gtattataat caccagcaac atgtttggtg atatcatctc cgatgaagcc
     6541 tccgttatcc caggttcctt gggtttgttg ccatctgcgt ccttggcctc tttgccagac
     6601 aagaacaccg catttggttt gtacgaacca tgccacggtt ctgctccaga tttgccaaag
     6661 aataaggtca accctatcgc cactatcttg tctgctgcaa tgatgttgaa attgtcattg
     6721 aacttgcctg aagaaggtaa ggccattgaa gatgcagtta aaaaggtttt ggatgcaggt
     6781 atcagaactg gtgatttagg tggttccaac agtaccaccg aagtcggtga tgctgtcgcc
     6841 gaagaagtta agaaaatcct tgcttaaaaa gattctcttt ttttatgata tttgtacata
     6901 aactttataa atgaaattca taatagaaac gacacgaaat tacaaaatgg aatatgttca
     6961 tagggtagac gaaactatat acgcaatcta catacattta tcaagaagga gaaaaaggag
     7021 gatgtaaagg aatacaggta agcaaattga tactaatggc tcaacgtgat aaggaaaaag
     7081 aattgcactt taacattaat attgacaagg aggagggcac cacacaaaaa gttaggtgta
     7141 acagaaaatc atgaaactat ga
//
