LOCUS       sequence-200171-       21637 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector containing Sr22 Schomburgk allele driven by maize 
            ubiquitin promoter, Sr22 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21637)
  AUTHORS   Md Hatta MA, Ghosh S, Athiyannan N, Richardson T, Steuernagel B, Yu 
            G, Rouse MN, Ayliffe M, Lagudah ES, Radhakrishnan GV, Periyannan SK,
            Wulff BBH
  TITLE     Extensive Genetic Variation at the Sr22 Wheat Stem Rust Resistance 
            Gene Locus in the Grasses Revealed Through Evolutionary Genomics and
            Functional Analyses.
  JOURNAL   Mol Plant Microbe Interact. 2020 Nov;33(11):1286-1298. doi: 
            10.1094/MPMI-01-20-0018-R. Epub 2020 Oct 1.
  PUBMED    32779520
REFERENCE   2  (bases 1 to 21637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-01-20-0018-R"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-11"; volume: "33"; issue: "11"; pages: 
            "1286-1298"
COMMENT     Sequence Label: pBW_0001
FEATURES             Location/Qualifiers
     source          1..21637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    416..440
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        1482..1827
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1770..1790
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    1857..1912
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             1914..2465
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      2488..3195
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(12795..12978)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(12831..13010)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    13134..13158
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      13646..14281
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(14956..15744)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      16841..17429
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     17330..17349
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     17583..17600
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(18789..19937)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     mobile_element  20562..21329
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     oriT            21389..21498
                     /note="incP origin of transfer"
ORIGIN
        1 gtcgacgatc gtcaacgttc acttctaaag aaatagcgcc actcagcttc ctcagcggct
       61 ttatccagcg atttcctatt atgtcggcat agttctcaag atcgacagcc tgtcacggtt
      121 aagcgagaaa tgaataagaa ggctgataat tcggatctct gcgagggaga tgatatttga
      181 tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc
      241 cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca
      301 aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg
      361 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca
      421 ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt
      481 ttttaatgta ctgaattcca atcccacaaa aatctgagct taacagcaca gttgctcctc
      541 tcagagcaga atcgggtatt caacaccctc atatcaacta ctacgttgtg tataacggtc
      601 cacatgccgg tatatacgat gactggggtt gtacaaaggc ggcaacaaac ggcgttcccg
      661 gagttgcaca caagaaattt gccactatta cagaggcaag agcagcagct gacgcgtaca
      721 caacaagtca gcaaacagac aggttgaact tcatccccaa aggagaagct caactcaagc
      781 ccaagagctt tgctaaggcc ctaacaagcc caccaaagca aaaagcccac tggctcacgc
      841 taggaaccaa aaggcccagc agtgatccag ccccaaaaga gatctccttt gccccggaga
      901 ttacaatgga cgatttcctc tatctttacg atctaggaag gaagttcgaa ggtgaaggtg
      961 acgacactat gttcaccact gataatgaga aggttagcct cttcaatttc agaaagaatg
     1021 ctgacccaca gatggttaga gaggcctacg cagcaggtct catcaagacg atctacccga
     1081 gtaacaatct ccaggagatc aaataccttc ccaagaaggt taaagatgca gtcaaaagat
     1141 tcaggactaa ttgcatcaag aacacagaga aagacatatt tctcaagatc agaagtacta
     1201 ttccagtatg gacgattcaa ggcttgcttc ataaaccaag gcaagtaata gagattggag
     1261 tctctaaaaa ggtagttcct actgaatcta aggccatgca tggagtctaa gattcaaatc
     1321 gaggatctaa cagaactcgc cgtgaagact ggcgaacagt tcatacagag tcttttacga
     1381 ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacactct ggtctactcc
     1441 aaaaatgtca aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg
     1501 ataatttcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg
     1561 acagtagaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggctatc
     1621 attcaagatc tctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
     1681 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgacatctcc
     1741 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
     1801 ggaagttcat ttcatttgga gaggacacgc tcgagtataa gagctctatt tttacaacaa
     1861 ttaccaacaa caacaaacaa caaacaacat tacaattact atttacaatt accatgagcc
     1921 cagaacgacg cccggccgac atccgccgtg ccaccgaggc ggacatgccg gcggtctgca
     1981 ccatcgtcaa ccactacatc gagacaagca cggtcaactt ccgtaccgag ccgcaggaac
     2041 cgcaggagtg gacggacgac ctcgtccgtc tgcgggagcg ctatccctgg ctcgtcgccg
     2101 aggtggacgg cgaggtcgcc ggcatcgcct acgcgggccc ctggaaggca cgcaacgcct
     2161 acgactggac ggccgagtca accgtgtacg tctccccccg ccaccagcgg acgggactgg
     2221 gctccacgct ctacacccac ctgctgaagt ccctggaggc acagggcttc aagagcgtgg
     2281 tcgctgtcat cgggctgccc aacgacccga gcgtgcgcat gcacgaggcg ctcggatatg
     2341 ccccccgcgg catgctgcgg gcggccggct tcaagcacgg gaactggcat gacgtgggtt
     2401 tctggcagct ggacttcagc ctgccggtac cgccccgtcc ggtcctgccc gtcaccgaga
     2461 tttgatgacc cgggggatcc tagagtcctg ctttaatgag atatgcgaga cgcctatgat
     2521 cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc
     2581 tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt tactatcgta
     2641 tttttatgaa taatattctc cgttcaattt actgattgta ccctactact tatatgtaca
     2701 atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc tatgatagag
     2761 cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa aaaagcggca
     2821 gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca aaagtgcccc
     2881 aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg aagggaactc
     2941 cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg ccgtccgctc
     3001 taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt aaccgacttg
     3061 ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg aagtgcaggt
     3121 caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt gcgacaacat
     3181 gtcgaggctc agcaggacct gcaggcatgc aagctctaga gcggccgcgt gcagcgtgac
     3241 ccggtcgtgc ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc
     3301 acatattttt tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt
     3361 aaactttact ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga
     3421 atcatataaa tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga
     3481 ctctacagtt ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca
     3541 cctatataat acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt
     3601 tttatagact aattttttta gtacatctat tttattctat tttagcctct aaattaagaa
     3661 aactaaaact ctattttagt ttttttattt aataatttag atataaaata gaataaaata
     3721 aagtgactaa aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt
     3781 tcttgtttcg agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc
     3841 aaccagcgaa ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt
     3901 cgctgcctct ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg
     3961 catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc
     4021 tcctctcacg gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt
     4081 cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg
     4141 ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct
     4201 tcaaggtacg ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc
     4261 cggtccatgg ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt
     4321 tgtgttagat ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt
     4381 ctgattgcta acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc
     4441 gcagacggga tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt
     4501 ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt
     4561 ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc
     4621 tgtttcaaac tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat
     4681 tcatagttac gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt
     4741 gatgcgggtt ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg
     4801 tggtgtggtt gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta
     4861 cctggtgtat ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga
     4921 gtttaagatg gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact
     4981 gatgcatata catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta
     5041 tctattataa taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg
     5101 gcatatgcag cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc
     5161 ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcaggaatg
     5221 atggcggaag ttctggtgag cgcatccaca ggggcaatgg gttccctcct gcggaagttg
     5281 ggagctatgt tgacggatga atacaagctg ctcaagaacg tccgtgggga catcaagttc
     5341 ctcaaggacg agctggaggt catgtgcgcg ttcctcctca agatgtcaga tgtggaggag
     5401 cctgacgaac caacaaagct gcgtgtgacg gcggtgcggg agatgtccta taagatagag
     5461 gacaacatcg acaagttcat ggtcctcgtg gaacaggaac acgggtcctc ctgttccgag
     5521 gcggctcatg gcgtcgccaa gctcatggac aagtgcaaga acttgctgcc ggacatcaag
     5581 gcccgccgca ggatcgccaa ggaggtcaaa gacatcaaga aagaaattaa ggatgtcagc
     5641 gacagatttt caaggtacca gatcttgttc tccttacagt actattagta cctaccatca
     5701 gatttgtgac cgatttgttg ccactcaagg tacaagattg acgactcctc ctcctctatg
     5761 ccggcaaaag acaaggtcga ccctcgactt cgtgcagtct acaaagacgc cgcagagctc
     5821 gtcggaattg atggaccaaa ggatgaactt gtcaagtggc tgaatgagaa agaaggccaa
     5881 tcactgaaat ctgtctctat tgttggatat ggagggttgg gcaagaccac gctcgccaac
     5941 cagatccggg tcaaccttgg agcgaccttc gactgcgggg cttttgtctc gatttcacgc
     6001 aagcctgaca tgaaggcgat attaagatct atattatcac aaatcaccaa gaaagacgat
     6061 gcttgctcta gattagatga tatacaactc atcatcgaca agattcgaga attcctccaa
     6121 gacacaaggt atgtatgtga gatctgctac gaagtaacat tgcttaatta gctcatgcat
     6181 aatcggttgc ctagaatttt gctaatgaag attgctttgt gtgatttact ttcgtcctca
     6241 acttgtatga ctgcgcttaa actttcaagt cattgtttct tgcaacttat gtcgatctgt
     6301 tcactaatta agatatagct aaaatgtgtt tgccgatttc tgtcgccaca cattttctaa
     6361 tttgattgcc acaagtttgt taaacttgcg gctatgttct gtttattaaa taattgtgac
     6421 atggcgtcag tcaaaacaat aaagtacatg caattccctc tcttttgtac aagataaata
     6481 tccgcacgca ctacatgact gcaaatatat ctatcttcta aggacccaaa gcacttgcat
     6541 gtgcagacca ttgttaccgg atatgcagta cattaggttt gtgattagcc actttaagaa
     6601 catagggttt ctagaggcta taaacctacg gttatcagta ttataatctt gaatggatat
     6661 caaacatccg ttgaagaatc tatgcccata acacaaaaag agaaaaggga agaattaatc
     6721 atagtcagat cggaccagcc tgactagcgt aacataatga caacagtagg ttgttgcgtc
     6781 tttcctggtt tcgtagcaat ttgcatgacg aattgaggcg cagagccacc actcaatatg
     6841 tggtcctacc aatcatgagg aaataacttg ttaccaaaat gacaaaagaa aataggtagt
     6901 ccccatcatt gtttcaatat ctatcctctt gcgaacatgc tattggcagt ggcaaaacat
     6961 tgtaccttgc cactggcaac agctgccttt actcggcctg agcattcata tttttttcct
     7021 cttggttgtt aaatcttgct atttttcgct tgccttctat ggcaatcttt ttttttctta
     7081 tattgccacc aaattgtatc taagagcaac tccaacacgc cgacgcaatt tttgtccatt
     7141 tagatcggcc aagcggacgg ggtgtctggt tttgcctttg ggtggtgcgc ctacgcatta
     7201 gtccgcgtgg gcatttaaaa aacttaaaaa gcttaaaata attaaatata aataaaaatg
     7261 catattaaag cccgtcaaag tccacataat taaacataat acattaatat attaataaaa
     7321 aacagccgcc ccagcccgca gccctaggcg tcttcgtcgg aggagtgggt gaggtcgaag
     7381 aatgccggca gctcagccca cgggaaggcc tgctggtggg cagccagcac cgcctgctcc
     7441 gctgtctgcg gcggcggcgg ccctacctgg caccgcgcac gcctcctcct cgcgctcctg
     7501 ccgctcctcc tcctcatcgc acgcccaacg aagttttggc gctcctcccc ctcgggttcc
     7561 cgctgcatgc ggacctggcc gtcgtaccgc tcctcctcca gcgtgcggtc cttccacgcc
     7621 acgaggtagg ccgcctcctc ctccatcacg cacagccaaa tgggcggctt gctcatccgc
     7681 ttgcagacga tgccctgcca ttggtaaacg ccggtgggcg cgggtggtgc tagcgctggt
     7741 gcaggcggtg acgatgtggc tggtggtccc ggcaggtaga cactatagct ggcggccgat
     7801 agctgcagcg tgtcatcgag gccagaccac taggagtcct cgcactaaga ctcctcggtg
     7861 acgtgatggg ccccctcctc caatgccttg aggaacgccg cctcctcctc aacctactat
     7921 ttgaccaccg gtggcggcgg tggagggcag gcttcgagct ggacggcgtc gcggaggcgc
     7981 acatggtgct cgatggtgaa ccagacttct cagttagggg agtctggcct cgatgggtgg
     8041 agtcgcgacg ctgctctggc gtcaggagag cacgccgggc ccgaatctcg ttggcgcgcg
     8101 cacgctcgga ctgtggcacc gccggcactg gaatgtggtg ggggtcgagg tgccagccgt
     8161 gggggaagtt cacgtccggc cacggcagtg agacgcggta ctcccggtgg tactaactac
     8221 gcgtggtgca ccgggatgta ctggtggtcc cgtggcgctg ccgccctccc atcccgcagc
     8281 gccgacacca atgcgaaccc cttcgctgcc ggcctcaccg gcccttgcaa cgagccggcc
     8341 tcgtggtcgt gcttgccttg cccttacctt tgccaaagaa acccatgacg gctagggttt
     8401 tctgtcaccg gcgagggagc agatgtggac tagagtggga ggagaagtgg atgaggcccg
     8461 ccccgccgca cccttgcaat taaaaagggc gtgatccgga cgtttctagt cactgccatg
     8521 cgggcccgag gtaggtgggc gccattgact acgtctgtgg tggtagttgg ctggcagaca
     8581 cgccagcccg aggcgcacac cgagcggacg cgcaacacgt ccgtctcgtg ttcgtgtgaa
     8641 cgcaaatcag gcacaaattt gggctggaaa tgcgtctaca tggatgccga acggacgtga
     8701 tttgggtttg tgtccgcacg ttgggccggc gtttttgtgc ctttcgaccc taatggacac
     8761 acacggacca aatccgttgg cgtgttagag ttgcttgtac ctagtactac tatgtgtgga
     8821 gttgttatct ttgagtttct cccccatctg tagctgtgta ttattgtgag atgctctcag
     8881 gatttcactt attttagata atgacaactt tcttgatgct agacaattac atcaaggtgt
     8941 attatatcta ggatgcacac agatgtgaga ttgtttcagg attttaggtt acttggtagg
     9001 tgttactata aatatctaat gagctctaat gtgtatgttc atgaaactgt gttgcaggta
     9061 ctttatcata attgatgata tatgggagtt aggaacatgg gaaactttga aatgtgcatt
     9121 tgtcaagaat acattgggca gcagaataat tatcacaaca cgtatagttg atgttgccaa
     9181 atcttgttct ccttctagtg aagatcttgt ctatgagatg aaaccactca gtgaggctga
     9241 ctcgaagaaa ttgtttttca agaggatatt tggctgtgaa gaaagctgtc ctgatagctt
     9301 gaaagaagct gccaatgata ttttgaaaaa atgccgtggc ctacctctgg ccatcaatgc
     9361 catttctagc gtgttggtca caacaaggga aacaaaagaa gagtgggatc gggtgcgaca
     9421 ttcaatacgc tcttcaaagg tcaaaagtga tataatagaa accatgaatt acatattgtc
     9481 tcttagttat tttgatcttc cccaccacct aagaagctgc ttattgtacc tggctctgtt
     9541 tcctgaagac caattgattg gaaggaagcg gttagtacgc agatggattt ctgaaggctt
     9601 tatccatgga gaaagtggac aagatctcat ggaattagga gaggaatatt ttcaccagct
     9661 tgtgaacaga agtctaatac agcccggcaa catagggtat gacggcaagg caatgtattg
     9721 ccgagtccat gacaccatcc ttgatttcct aattgacaaa tcctctgaag agaacatgtg
     9781 tactgtccta aagaaacaat gcaagcctaa tggcatagtt cgtcggctct ctctaatggg
     9841 gaatgaggat gaagaaatcg tggagcaatt ggatctgtca cacgctcggt caatcactgc
     9901 ttttggggat atcaagctat tgccttcgct tgggaggtca aaatgcttgc gcgtgctaga
     9961 cttgcaagac tgcgatcagt tggaaaatca tcatatcaag gatattgaaa gactctatca
    10021 gctaagatac ttggatatct cttctacagg aatcacggag cttcccaggc aaattgggga
    10081 gttgctgtat ctagagacgc tagttgccta tggattacgt gagcttcctg aaagcacaag
    10141 tcggctacaa cgactggcac gtttgtttgt ttactctggt tgtaagcttc caggtgggtt
    10201 gggaaacttg ataaacttgc aagagcttga ctgcgttgat gccttgcatt tgaagcatgt
    10261 ggaagagctc ggcaaactaa caaatctgag aaagctgagt attaaattgg acactggtgg
    10321 gattgaaggc aacaaattag aagagtccaa ggaaaagctg gtgtcctctc tctgtaaact
    10381 ggacgaatgc ggcctccttt ccctgagtat tgattattat ctgagagaaa aggatggaga
    10441 agagccgttc ctccctgctt tgggttgtat ccaggaggtt tttgtatatg gacaagatat
    10501 cagccggatt agcagatggc tcgcgtcact tcccaaccta cacaggttgc ttttggacga
    10561 tccgaagata gagcagcagg atattgagat gattggattg atacccaatc tgatcgattt
    10621 gacactgccg cctctataca aaacagacga cgctgggcgg ctcatcatca ggcgtgaagg
    10681 atttcaacag ttgcagaagt ttgaggctta caatactcgt atgggggttt taatgtttga
    10741 accgggggct atgccgaggc tcaaggagct taaactccat aacttcatcg aaaagcccaa
    10801 atctgctgcg gtcgatttcg actttggcat ccaacgcctc tccagccttg ctcgcctcac
    10861 tgtcagctta tcctgcggtg gctggacggt ggcagaagta gaggctgcgg aggatgcttt
    10921 caagagcatg gccgaggcga accccaaccg tccaatactg gagatgacaa gatataatac
    10981 gcagcatatg ttacaagacg agcaaatagg tatgacaggc tcaggtacta ttaactagtt
    11041 tgtacttttg tcttggtgag cagtagatca aaagatgtgc atatgcttca atcttttttc
    11101 cggtaatcta tttcagctac aacgccggcc gtcaagtcct aggctttgcc taaaccgaga
    11161 tgcgcctttg gttatggagc ccaagtcacg tactcggatt ggatgctgca gcaaatgata
    11221 cttctgtaca tgtctgcaag tatcacgaat tagtggaatg actaggaata agtaagacaa
    11281 gtatcacgaa ttcagagtcg gatgcaattt ttttcttttc ttttgggcat aagtagattg
    11341 tcatttgagt atttttttcc attaatattt gtgtattctt agattgagac tttgatatgt
    11401 aacccagatt tctgttgtta ggaaacacat accgtgtagc ttatcagaag aaaaagatct
    11461 agcgtgcatc tatattctat cggactatga tatttggcac atgtgtgcca tataaacaaa
    11521 actgtcacac ctctatttcc ttcactctaa atagatgtat acattagttt gattctttct
    11581 tctttcacgt gcatttctcc ttctaattag atgcatacat tagaggacaa aaaagacgat
    11641 gtcattctag attttcttta ttaaaatatt taaaatgttt tgtagctcaa accgtcggtc
    11701 cgatgtaaaa accgttttta catatacgat tcgtcgcgat gagatctttg aaactagatc
    11761 ctatgttgac atgtttcgat gacttttttt aaccaaaatt taccatgcct tttatatgca
    11821 agttaccgtg ctatttccac ataagtcatc ggaggcataa aaatggttca ccgacactac
    11881 agttcatata ttaccatgat gtttacatat aagttaccgg gagggggggg gggggtgtct
    11941 gttttcaata atttccccac cgagtcaaat attaccatag tatatgtacg taagttatca
    12001 ggtatccgat gggtatatta ccatggctat cttttaaaaa aatctctaat ttaccgcggt
    12061 gtttgtatct aaattatcaa gtttgtggtg cctaatttac tatgctattt acatacaaaa
    12121 gttaccagtg tattttttga caatttttta ctattcttcc ccaaatcaag actattaccg
    12181 tgctgtttac acacaagtta ccgggtatat tttcaacaac ttcatccacc cgaatcaaac
    12241 ttatcatgat gtttgtgagt aacttatcat gtctgcaatg cgtatattac cattctattt
    12301 aaacaaaatt taccaacgat atgcttcaaa caatttttta cgtgggtcaa agctagcata
    12361 ctgtttttgc gtaagttatt gtatctgcaa taaatatatt accgtgctat ttacctagaa
    12421 gttattgggg gtaatgttct tcaacgaaaa aaaattcagg gtcaaaaaat tattgtggtg
    12481 tttgaacgag agttattagc tctccgatgg gtaaattatc atgttatttg cacataaatt
    12541 accgagggta tgcttttcaa caacttcctc catccaggtc aaagttatca tggtgttttt
    12601 gctcaagttt tcaagtttgt ggtgcgtata ttaccacgat gttgactgtt tacaaggttt
    12661 ttgagtcgcc gactaatcgc cgactaatcg tcgagtcgtc gcggcggcgg ccgccaccgc
    12721 ggctgagtgg ctccttcaag cttgcggatc cgggcggaaa taggtaaaga agttgcggat
    12781 aaggtaattg ccattgcaga ttatttggat tgagagtgaa tatgagactc taattggata
    12841 ccgaggggaa tttatggaac gtcagtggag catttttgac aagaaatatt tgctagctga
    12901 tagtgacctt aggcgacttt tgaacgcgca ataatggttt ctgacgtatg tgcttagctc
    12961 attaaactcc agaaacccgc ggctgagtgg ctccttcaac gttgcggttc tgtcagttcc
    13021 aaacgtaaaa cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact cccttaattc
    13081 tccgctcatg atcagattgt cgtttcccgc cttcagttta aactatcagt gtttgacagg
    13141 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa
    13201 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc
    13261 cctcgggagt gcttggcatt ccgtgcgata atgacttctg ttcaaccacc caaacgtcgg
    13321 aaagcctgac gacggagcag cattccaaaa agatcccttg gctcgtctgg gtcggctaga
    13381 aggtcgagtg ggctgctgtg gcttgatccc tcaacgcggt cgcggacgta gcgcagcgcc
    13441 gaaaaatcct cgatcgcaaa tccgacgctg tcgaaaagcg tgatctgctt gtcgctcttt
    13501 cggccgacgt cctggccagt catcacgcgc caaagttccg tcacaggatg atctggcgcg
    13561 agttgctgga tctcgccttc aatccgggtc tgtggcggga actccacgaa aatatccgaa
    13621 cgcagcaaga tcgtcgaccc tttccgacgc tcaccgggct ggttgccctc gccgctgggc
    13681 tggcggccgt ctatggccct gcaaacgcgc cagaaacgcc gtcgaagccg tgtgcgagac
    13741 accgcggccg gccgccggcg ttgtggatac ctcgcggaaa acttggccct cactgacaga
    13801 tgaggggcgg acgttgacac ttgaggggcc gactcacccg gcgcggcgtt gacagatgag
    13861 gggcaggctc gatttcggcc ggcgacgtgg agctggccag cctcgcaaat cggcgaaaac
    13921 gcctgatttt acgcgagttt cccacagatg atgtggacaa gcctggggat aagtgccctg
    13981 cggtattgac acttgagggg cgcgactact gacagatgag gggcgcgatc cttgacactt
    14041 gaggggcaga gtgctgacag atgaggggcg cacctattga catttgaggg gctgtccaca
    14101 ggcagaaaat ccagcatttg caagggtttc cgcccgtttt tcggccaccg ctaacctgtc
    14161 ttttaacctg cttttaaacc aatatttata aaccttgttt ttaaccaggg ctgcgccctg
    14221 tgcgcgtgac cgcgcacgcc gaaggggggt gccccccctt ctcgaaccct cccggcccgc
    14281 taacgcgggc ctcccatccc cccaggggct gcgcccctcg gccgcgaacg gcctcacccc
    14341 aaaaatggca gccaagctcc taacatttta ttagagagca ggctagttgc ttagatacat
    14401 gatcttcagg ccgttatctg tcagggcaag cgaaaattgg ccatttatga cgaccaatgc
    14461 cccgcagaag ctcccatctt tgccgccata gacgccgcgc cccccttttg gggtgtagaa
    14521 catccttttg ccagatgtgg aaaagaagtt cgttgtccca ttgttggcaa tgacgtagta
    14581 gccggcgaaa gtgcgagacc catttgcgct atatataagc ctacgatttc cgttgcgact
    14641 attgtcgtaa ttggatgaac tattatcgta gttgctctca gagttgtcgt aatttgatgg
    14701 actattgtcg taattgctta tggagttgtc gtagttgctt ggagaaatgt cgtagttgga
    14761 tggggagtag tcatagggaa gacgagcttc atccactaaa acaattggca ggtcagcaag
    14821 tgcctgcccc gatgccatcg caagtacgag gcttagaacc accttcaaca gatcgcgcat
    14881 agtcttcccc agctctctaa cgcttgagtt aagccgcgcc gcgaagcggc gtcggcttga
    14941 acgaattgtt agacattatt tgccgactac cttggtgatc tcgcctttca cgtagtgaac
    15001 aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttgtccaa gataagcctg
    15061 cctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc
    15121 agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt
    15181 aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt
    15241 ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc
    15301 tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat
    15361 gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa
    15421 ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt
    15481 gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc
    15541 gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc
    15601 aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag
    15661 caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac
    15721 ttcggcgatc accgcttccc tcatgatgtt taactcctga attaagccgc gccgcgaagc
    15781 ggtgtcggct tgaatgaatt gttaggcgtc atcctgtgct cccgagaacc agtaccagta
    15841 catcgctgtt tcgttcgaga cttgaggtct agttttatac gtgaacaggt caatgccgcc
    15901 gagagtaaag ccacattttg cgtacaaatt gcaggcaggt acattgttcg tttgtgtctc
    15961 taatcgtatg ccaaggagct gtctgcttag tgcccacttt ttcgcaaatt cgatgagact
    16021 gtgcgcgact cctttgcctc ggtgcgtgtg cgacacaaca atgtgttcga tagaggctag
    16081 atcgttccat gttgagttga gttcaatctt cccgacaagc tcttggtcga tgaatgcgcc
    16141 atagcaagca gagtcttcat cagagtcatc atccgagatg taatccttcc ggtaggggct
    16201 cacacttctg gtagatagtt caaagccttg gtcggatagg tgcacatcga acacttcacg
    16261 aacaatgaaa tggttctcag catccaatgt ttccgccacc tgctcaggga tcaccgaaat
    16321 cttcatatga cgcctaacgc ctggcacagc ggatcgcaaa cctggcgcgg cttttggcac
    16381 aaaaggcgtg acaggtttgc gaatccgttg ctgccacttg tttaatagac tggatggagg
    16441 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
    16501 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
    16561 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
    16621 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
    16681 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
    16741 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
    16801 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgatatcct ttttttctgc
    16861 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    16921 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    16981 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    17041 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    17101 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    17161 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    17221 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    17281 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    17341 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    17401 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    17461 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    17521 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
    17581 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
    17641 atctgtgcgg tatttcacac cgcatatgaa gatcggcggc aatagcttct tagcgccatc
    17701 ccggctgaga aagcccagta aggaaacaac tgtaggttcg agtcgcgaga tcccccggaa
    17761 ccaaaggaag taggttaaac ccgctccgat caggccgagc cacgccaggc cgagaacatt
    17821 ggttcctgta ggcatcggga ttggcggatc aaacactaaa gctactggaa cgagcagaag
    17881 tcctccggcc gccagttgcc aggcggtaaa ggtgagcaga ggcacgggag gttgccactt
    17941 gcgggtcagc acggttccga acgccatgga aaccgccccc gccaggcccg ctgcgacgcc
    18001 gacaggatct agcgctgcgt ttggtgtcaa caccaacagc gccacgcccg cagttccgca
    18061 aatagccccc aggaccgcca tcaatcgtat cgggctacct agcagagcgg cagagatgaa
    18121 cacgaccatc agcggctgca cagcgcctac cgtcgccgcg accccgcccg gcaggcggta
    18181 gaccgaaata aacaacaagc tccagaatag cgaaatatta agtgcgccga ggatgaagat
    18241 gcgcatccac cagattcccg ttggaatctg tcggacgatc atcacgagca ataaacccgc
    18301 cggcaacgcc cgcagcagca taccggcgac ccctcggcct cgctgttcgg gctccacgaa
    18361 aacgccggac agatgcgcct tgtgagcgtc cttggggccg tcctcctgtt tgaagaccga
    18421 cagcccaatg atctcgccgt cgatgtaggc gccgaatgcc acggcatctc gcaaccgttc
    18481 agcgaacgcc tccatgggct ttttctcctc gtgctcgtaa acggacccga acatctctgg
    18541 agctttcttc agggccgaca atcggatctc gcggaaatcc tgcacgtcgg ccgctccaag
    18601 ccgtcgaatc tgagccttaa tcacaattgt caattttaat cctctgttta tcggcagttc
    18661 gtagagcgcg ccgtgcgtcc cgagcgatac tgagcgaagc aagtgcgtcg agcagtgccc
    18721 gcttgttcct gaaatgccag taaagcgctg gctgctgaac ccccagccgg aactgacccc
    18781 acaaggccct agcgtttgca atgcaccagg tcatcattga cccaggcgtg ttccaccagg
    18841 ccgctgcctc gcaactcttc gcaggcttcg ccgacctgct cgcgccactt cttcacgcgg
    18901 gtggaatccg atccgcacat gaggcggaag gtttccagct tgagcgggta cggctcccgg
    18961 tgcgagctga aatagtcgaa catccgtcgg gccgtcggcg acagcttgcg gtacttctcc
    19021 catatgaatt tcgtgtagtg gtcgccagca aacagcacga cgatttcctc gtcgatcagg
    19081 acctggcaac gggacgtttt cttgccacgg tccaggacgc ggaagcggtg cagcagcgac
    19141 accgattcca ggtgcccaac gcggtcggac gtgaagccca tcgccgtcgc ctgtaggcgc
    19201 gacaggcatt cctcggcctt cgtgtaatac cggccattga tcgaccagcc caggtcctgg
    19261 caaagctcgt agaacgtgaa ggtgatcggc tcgccgatag gggtgcgctt cgcgtactcc
    19321 aacacctgct gccacaccag ttcgtcatcg tcggcccgca gctcgacgcc ggtgtaggtg
    19381 atcttcacgt ccttgttgac gtggaaaatg accttgtttt gcagcgcctc gcgcgggatt
    19441 ttcttgttgc gcgtggtgaa cagggcagag cgggccgtgt cgtttggcat cgctcgcatc
    19501 gtgtccggcc acggcgcaat atcgaacaag gaaagctgca tttccttgat ctgctgcttc
    19561 gtgtgtttca gcaacgcggc ctgcttggcc tcgctgacct gttttgccag gtcctcgccg
    19621 gcggtttttc gcttcttggt cgtcatagtt cctcgcgtgt cgatggtcat cgacttcgcc
    19681 aaacctgccg cctcctgttc gagacgacgc gaacgctcca cggcggccga tggcgcgggc
    19741 agggcagggg gagccagttg cacgctgtcg cgctcgatct tggccgtagc ttgctggacc
    19801 atcgagccga cggactggaa ggtttcgcgg ggcgcacgca tgacggtgcg gcttgcgatg
    19861 gtttcggcat cctcggcgga aaaccccgcg tcgatcagtt cttgcctgta tgccttccgg
    19921 tcaaacgtcc gattcattca ccctccttgc gggattgccc cgactcacgc cggggcaatg
    19981 tgcccttatt cctgatttga cccgcctggt gccttggtgt ccagataatc caccttatcg
    20041 gcaatgaagt cggtcccgta gaccgtctgg ccgtccttct cgtacttggt attccgaatc
    20101 ttgccctgca cgaataccag ctccgcgaag tcgctcttct tgatggagcg catggggacg
    20161 tgcttggcaa tcacgcgcac cccccggccg ttttagcggc taaaaaagtc atggctctgc
    20221 cctcgggcgg accacgccca tcatgacctt gccaagctcg tcctgcttct cttcgatctt
    20281 cgccagcagg gcgaggatcg tggcatcacc gaaccgcgcc gtgcgcgggt cgtcggtgag
    20341 ccagagtttc agcaggccgc ccaggcggcc caggtcgcca ttgatgcggg ccagctcgcg
    20401 gacgtgctca tagtccacga cgcccgtgat tttgtagccc tggccgacgg ccagcaggta
    20461 ggccgacagg ctcatgccgg ccgccgccgc cttttcctca atcgctcttc gttcgtctgg
    20521 aaggcagtac accttgatag gtgggctgcc cttcctggtt gggtaatgac tccaacttat
    20581 tgatagtgtt ttatgttcag ataatgcccg atgactttgt catgcagctc caccgatttt
    20641 gagaacgaca gcgacttccg tcccagccgt gccaggtgct gcctcagatt caggttatgc
    20701 cgctcaattc gctgcgtata tcgcttgctg attacgtgca gctttccctt caggcgggat
    20761 tcatacagcg gccagccatc cgtcatccat atcaccacgt caaagggtga cagcaggctc
    20821 ataagacgcc ccagcgtcgc catagtgcgt tcaccgaata cgtgcgcaac aaccgtcttc
    20881 cggagactgt catacgcgta aaacagccag cgctggcgcg atttagcccc gacatagccc
    20941 cactgttcgt ccatttccgc gcagacgatg acgtcactgc ccggctgtat gcgcgaggtt
    21001 accgactgcg gcctgagttt tttaagtgac gtaaaatcgt gttgaggcca acgcccataa
    21061 tgcgggctgt tgcccggcat ccaacgccat tcatggccat atcaatgatt ttctggtgcg
    21121 taccgggttg agaagcggtg taagtgaact gcagttgcca tgttttacgg cagtgagagc
    21181 agagatagcg ctgatgtccg gcggtgcttt tgccgttacg caccaccccg tcagtagctg
    21241 aacaggaggg acagctgata gaaacagaag ccactggagc acctcaaaaa caccatcata
    21301 cactaaatca gtaagttggc agcatcaccc ctggttggct tggtttcatc agccatccgc
    21361 ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg
    21421 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
    21481 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
    21541 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata
    21601 atgaccccga agcagggtta tgcagcggaa aagatcc
//
