LOCUS       sequence-200179-       21891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector containing Sr22 Schomburgk allele driven by Sr33 
            promoter, Sr33 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21891)
  AUTHORS   Md Hatta MA, Ghosh S, Athiyannan N, Richardson T, Steuernagel B, Yu 
            G, Rouse MN, Ayliffe M, Lagudah ES, Radhakrishnan GV, Periyannan SK,
            Wulff BBH
  TITLE     Extensive Genetic Variation at the Sr22 Wheat Stem Rust Resistance 
            Gene Locus in the Grasses Revealed Through Evolutionary Genomics and
            Functional Analyses.
  JOURNAL   Mol Plant Microbe Interact. 2020 Nov;33(11):1286-1298. doi: 
            10.1094/MPMI-01-20-0018-R. Epub 2020 Oct 1.
  PUBMED    32779520
REFERENCE   2  (bases 1 to 21891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-01-20-0018-R"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-11"; volume: "33"; issue: "11"; pages: 
            "1286-1298"
COMMENT     Sequence Label: pBW_0002
FEATURES             Location/Qualifiers
     source          1..21891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    416..440
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        1482..1827
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1770..1790
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    1857..1912
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             1914..2465
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      2488..3195
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(13049..13232)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(13085..13264)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    13388..13412
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      13900..14535
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(15210..15998)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      17095..17683
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     17584..17603
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     17837..17854
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(19043..20191)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     mobile_element  20816..21583
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     oriT            21643..21752
                     /note="incP origin of transfer"
ORIGIN
        1 gtcgacgatc gtcaacgttc acttctaaag aaatagcgcc actcagcttc ctcagcggct
       61 ttatccagcg atttcctatt atgtcggcat agttctcaag atcgacagcc tgtcacggtt
      121 aagcgagaaa tgaataagaa ggctgataat tcggatctct gcgagggaga tgatatttga
      181 tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc
      241 cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca
      301 aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg
      361 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca
      421 ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt
      481 ttttaatgta ctgaattcca atcccacaaa aatctgagct taacagcaca gttgctcctc
      541 tcagagcaga atcgggtatt caacaccctc atatcaacta ctacgttgtg tataacggtc
      601 cacatgccgg tatatacgat gactggggtt gtacaaaggc ggcaacaaac ggcgttcccg
      661 gagttgcaca caagaaattt gccactatta cagaggcaag agcagcagct gacgcgtaca
      721 caacaagtca gcaaacagac aggttgaact tcatccccaa aggagaagct caactcaagc
      781 ccaagagctt tgctaaggcc ctaacaagcc caccaaagca aaaagcccac tggctcacgc
      841 taggaaccaa aaggcccagc agtgatccag ccccaaaaga gatctccttt gccccggaga
      901 ttacaatgga cgatttcctc tatctttacg atctaggaag gaagttcgaa ggtgaaggtg
      961 acgacactat gttcaccact gataatgaga aggttagcct cttcaatttc agaaagaatg
     1021 ctgacccaca gatggttaga gaggcctacg cagcaggtct catcaagacg atctacccga
     1081 gtaacaatct ccaggagatc aaataccttc ccaagaaggt taaagatgca gtcaaaagat
     1141 tcaggactaa ttgcatcaag aacacagaga aagacatatt tctcaagatc agaagtacta
     1201 ttccagtatg gacgattcaa ggcttgcttc ataaaccaag gcaagtaata gagattggag
     1261 tctctaaaaa ggtagttcct actgaatcta aggccatgca tggagtctaa gattcaaatc
     1321 gaggatctaa cagaactcgc cgtgaagact ggcgaacagt tcatacagag tcttttacga
     1381 ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacactct ggtctactcc
     1441 aaaaatgtca aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg
     1501 ataatttcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg
     1561 acagtagaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggctatc
     1621 attcaagatc tctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
     1681 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgacatctcc
     1741 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
     1801 ggaagttcat ttcatttgga gaggacacgc tcgagtataa gagctctatt tttacaacaa
     1861 ttaccaacaa caacaaacaa caaacaacat tacaattact atttacaatt accatgagcc
     1921 cagaacgacg cccggccgac atccgccgtg ccaccgaggc ggacatgccg gcggtctgca
     1981 ccatcgtcaa ccactacatc gagacaagca cggtcaactt ccgtaccgag ccgcaggaac
     2041 cgcaggagtg gacggacgac ctcgtccgtc tgcgggagcg ctatccctgg ctcgtcgccg
     2101 aggtggacgg cgaggtcgcc ggcatcgcct acgcgggccc ctggaaggca cgcaacgcct
     2161 acgactggac ggccgagtca accgtgtacg tctccccccg ccaccagcgg acgggactgg
     2221 gctccacgct ctacacccac ctgctgaagt ccctggaggc acagggcttc aagagcgtgg
     2281 tcgctgtcat cgggctgccc aacgacccga gcgtgcgcat gcacgaggcg ctcggatatg
     2341 ccccccgcgg catgctgcgg gcggccggct tcaagcacgg gaactggcat gacgtgggtt
     2401 tctggcagct ggacttcagc ctgccggtac cgccccgtcc ggtcctgccc gtcaccgaga
     2461 tttgatgacc cgggggatcc tagagtcctg ctttaatgag atatgcgaga cgcctatgat
     2521 cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc
     2581 tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt tactatcgta
     2641 tttttatgaa taatattctc cgttcaattt actgattgta ccctactact tatatgtaca
     2701 atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc tatgatagag
     2761 cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa aaaagcggca
     2821 gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca aaagtgcccc
     2881 aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg aagggaactc
     2941 cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg ccgtccgctc
     3001 taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt aaccgacttg
     3061 ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg aagtgcaggt
     3121 caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt gcgacaacat
     3181 gtcgaggctc agcaggacct gcaggcatgc aagctctaga gcggccgcga agacaatctg
     3241 agcgttagga gggagctggc ggcgtcgaac tccttttcct acatacaccc ttttttgacg
     3301 ggacatttac accgtttttt taatgtatat gtgacagaaa catgttggta gaatttgggc
     3361 ttcatttcgt tttgcaatgg cccttctcaa aaatatactt gtagctggaa gcaccctttt
     3421 atttgtatgt aacggaaaca cattggcaga actagtaaat aatatttatt tttgaactcg
     3481 ggtaatcccc gtaatgcaaa tatacacaag tctgaataga cgtttaggag gtgggaaaga
     3541 gaaagatcaa gttcaactta tcgccaggaa accgctgcga gtgcgcgcaa tggacactca
     3601 ccatggggcg aaaacccaat gacaccaaac tatactaaca atgccataca aagctcatgc
     3661 agtagcagca gcggccaaag gtaccaggag tctgaagcag cacctaaaca gcgacaaacc
     3721 actacaaaac atgcagagct gtatgagcca gagcaagtaa atattttctg aagtaaggaa
     3781 tccaacaatg agtcgcagtt gtttacgaat taaggagctc gggtgagccc agaatttcag
     3841 tcattacaaa catgatacta caccctcagg tttgtccggt tcagataaaa aaaacatcaa
     3901 catgacatga gccagccatt cgatgtaaca gacagcaaca acgatggctc tcagccgaaa
     3961 cagagcaact tactacaacg ctttactaaa aaaccaagca acatctcttt atgcaagcga
     4021 ctgccattta aaaggagcac atcacacaac ctctcggaac ctttttatcc ttcaattttt
     4081 tgcatgaccg gtgcccaccc aacgtttatg aaacatctca gacgccacta gcagtaacct
     4141 gcgcccctgg tacctgcaca acgcaagtct ttcttctgta agctatacca acagaacatc
     4201 caaaatgcaa ctttaccaac tacacagctt aggtacaaat ggaaactatt tctgaagcaa
     4261 gcatcatttc ctcattagaa ccaccctgaa attcatgaac caatcacaca gtctgtccat
     4321 gctaacagga actatatcaa aactaaaagc acacagcaat catagagtgc ccccaagtat
     4381 tacgagcaat cgaacaatta agaaggtatg atccactgac tcttttctac tgcgaaaagg
     4441 taaattgtgc caagtgcatt tttctcagat acttatgcca aagaaacttc tgtaaaatta
     4501 cctttgcctg ttcagaattt ccgtagctgc tagagaagca caattgaaca atgcaatgac
     4561 aagctgagct ctagagatgg aaaaaaaggt ggagaataat atgatgagta aatatgtata
     4621 gattgagagc acacctgaag caacgtcaga agcctaggac ttgacggccg gcgttgtagc
     4681 tgaaatagat taccggaaaa aagattgaag catatgcaca tcttttgatc tactgctcac
     4741 caagacaaaa gtacaaacta gttaatagta cctgagcctg tcatacctat ttgctcgtct
     4801 tgtaacatat gctgcgtatt atatcttgtc atctccagta ttggacggtt ggggttcgcc
     4861 tcggccatgc tcttgaaagc atcctccgca gcctctactt ctgccaccgt ccagccaccg
     4921 caggataagc tgacagtgag gcgagcaagg ctggagaggc gttggatgcc aaagtcgaaa
     4981 tcgaccgcag cagatttggg cttttcgatg aagttatgga gtttaagctc cttgagcctc
     5041 ggcatagccc ccggttcaaa cattaaaacc cccatacgag tattgtaagc ctcaaacttc
     5101 tgcaactgtt gaaatccttc acgcctgatg atgagccgcc cagcgtcgtc tgttttgtat
     5161 agaggcggca gtgtcaaatc gatcagattg ggtatcaatc caatcatctc aatatcctgc
     5221 tgctctatct tcggatcgtc caaaagcaac ctgtgtaggt tgggaagtga cgcgagccat
     5281 ctgctaatcc ggctgatatc ttgtccatat acaaaaacct cctggataca acccaaagca
     5341 gggaggaacg gctcttctcc atccttttct ctcagataat aatcaatact cagggaaagg
     5401 aggccgcatt cgtccagttt acagagagag gacaccagct tttccttgga ctcttctaat
     5461 ttgttgcctt caatcccacc agtgtccaat ttaatactca gctttctcag atttgttagt
     5521 ttgccgagct cttccacatg cttcaaatgc aaggcatcaa cgcagtcaag ctcttgcaag
     5581 tttatcaagt ttcccaaccc acctggaagc ttacaaccag agtaaacaaa caaacgtgcc
     5641 agtcgttgta gccgacttgt gctttcagga agctcacgta atccataggc aactagcgtc
     5701 tctagataca gcaactcccc aatttgcctg ggaagctccg tgattcctgt agaagagata
     5761 tccaagtatc ttagctgata gagtctttca atatccttga tatgatgatt ttccaactga
     5821 tcgcagtctt gcaagtctag cacgcgcaag cattttgacc tcccaagcga aggcaatagc
     5881 ttgatatccc caaaagcagt gattgaccga gcgtgtgaca gatccaattg ctccacgatt
     5941 tcttcatcct cattccccat tagagagagc cgacgaacta tgccattagg cttgcattgt
     6001 ttctttagga cagtacacat gttctcttca gaggatttgt caattaggaa atcaaggatg
     6061 gtgtcatgga ctcggcaata cattgccttg ccgtcatacc ctatgttgcc gggctgtatt
     6121 agacttctgt tcacaagctg gtgaaaatat tcctctccta attccatgag atcttgtcca
     6181 ctttctccat ggataaagcc ttcagaaatc catctgcgta ctaaccgctt ccttccaatc
     6241 aattggtctt caggaaacag agccaggtac aataagcagc ttcttaggtg gtggggaaga
     6301 tcaaaataac taagagacaa tatgtaattc atggtttcta ttatatcact tttgaccttt
     6361 gaagagcgta ttgaatgtcg cacccgatcc cactcttctt ttgtttccct tgttgtgacc
     6421 aacacgctag aaatggcatt gatggccaga ggtaggccac ggcatttttt caaaatatca
     6481 ttggcagctt ctttcaagct atcaggacag ctttcttcac agccaaatat cctcttgaaa
     6541 aacaatttct tcgagtcagc ctcactgagt ggtttcatct catagacaag atcttcacta
     6601 gaaggagaac aagatttggc aacatcaact atacgtgttg tgataattat tctgctgccc
     6661 aatgtattct tgacaaatgc acatttcaaa gtttcccatg ttcctaactc ccatatatca
     6721 tcaattatga taaagtacct gcaacacagt ttcatgaaca tacacattag agctcattag
     6781 atatttatag taacacctac caagtaacct aaaatcctga aacaatctca catctgtgtg
     6841 catcctagat ataatacacc ttgatgtaat tgtctagcat caagaaagtt gtcattatct
     6901 aaaataagtg aaatcctgag agcatctcac aataatacac agctacagat gggggagaaa
     6961 ctcaaagata acaactccac acatagtagt actaggtaca agcaactcta acacgccaac
     7021 ggatttggtc cgtgtgtgtc cattagggtc gaaaggcaca aaaacgccgg cccaacgtgc
     7081 ggacacaaac ccaaatcacg tccgttcggc atccatgtag acgcatttcc agcccaaatt
     7141 tgtgcctgat ttgcgttcac acgaacacga gacggacgtg ttgcgcgtcc gctcggtgtg
     7201 cgcctcgggc tggcgtgtct gccagccaac taccaccaca gacgtagtca atggcgccca
     7261 cctacctcgg gcccgcatgg cagtgactag aaacgtccgg atcacgccct ttttaattgc
     7321 aagggtgcgg cggggcgggc ctcatccact tctcctccca ctctagtcca catctgctcc
     7381 ctcgccggtg acagaaaacc ctagccgtca tgggtttctt tggcaaaggt aagggcaagg
     7441 caagcacgac cacgaggccg gctcgttgca agggccggtg aggccggcag cgaaggggtt
     7501 cgcattggtg tcggcgctgc gggatgggag ggcggcagcg ccacgggacc accagtacat
     7561 cccggtgcac cacgcgtagt tagtaccacc gggagtaccg cgtctcactg ccgtggccgg
     7621 acgtgaactt cccccacggc tggcacctcg acccccacca cattccagtg ccggcggtgc
     7681 cacagtccga gcgtgcgcgc gccaacgaga ttcgggcccg gcgtgctctc ctgacgccag
     7741 agcagcgtcg cgactccacc catcgaggcc agactcccct aactgagaag tctggttcac
     7801 catcgagcac catgtgcgcc tccgcgacgc cgtccagctc gaagcctgcc ctccaccgcc
     7861 gccaccggtg gtcaaatagt aggttgagga ggaggcggcg ttcctcaagg cattggagga
     7921 gggggcccat cacgtcaccg aggagtctta gtgcgaggac tcctagtggt ctggcctcga
     7981 tgacacgctg cagctatcgg ccgccagcta tagtgtctac ctgccgggac caccagccac
     8041 atcgtcaccg cctgcaccag cgctagcacc acccgcgccc accggcgttt accaatggca
     8101 gggcatcgtc tgcaagcgga tgagcaagcc gcccatttgg ctgtgcgtga tggaggagga
     8161 ggcggcctac ctcgtggcgt ggaaggaccg cacgctggag gaggagcggt acgacggcca
     8221 ggtccgcatg cagcgggaac ccgaggggga ggagcgccaa aacttcgttg ggcgtgcgat
     8281 gaggaggagg agcggcagga gcgcgaggag gaggcgtgcg cggtgccagg tagggccgcc
     8341 gccgccgcag acagcggagc aggcggtgct ggctgcccac cagcaggcct tcccgtgggc
     8401 tgagctgccg gcattcttcg acctcaccca ctcctccgac gaagacgcct agggctgcgg
     8461 gctggggcgg ctgtttttta ttaatatatt aatgtattat gtttaattat gtggactttg
     8521 acgggcttta atatgcattt ttatttatat ttaattattt taagcttttt aagtttttta
     8581 aatgcccacg cggactaatg cgtaggcgca ccacccaaag gcaaaaccag acaccccgtc
     8641 cgcttggccg atctaaatgg acaaaaattg cgtcggcgtg ttggagttgc tcttagatac
     8701 aatttggtgg caatataaga aaaaaaaaga ttgccataga aggcaagcga aaaatagcaa
     8761 gatttaacaa ccaagaggaa aaaaatatga atgctcaggc cgagtaaagg cagctgttgc
     8821 cagtggcaag gtacaatgtt ttgccactgc caatagcatg ttcgcaagag gatagatatt
     8881 gaaacaatga tggggactac ctattttctt ttgtcatttt ggtaacaagt tatttcctca
     8941 tgattggtag gaccacatat tgagtggtgg ctctgcgcct caattcgtca tgcaaattgc
     9001 tacgaaacca ggaaagacgc aacaacctac tgttgtcatt atgttacgct agtcaggctg
     9061 gtccgatctg actatgatta attcttccct tttctctttt tgtgttatgg gcatagattc
     9121 ttcaacggat gtttgatatc cattcaagat tataatactg ataaccgtag gtttatagcc
     9181 tctagaaacc ctatgttctt aaagtggcta atcacaaacc taatgtactg catatccggt
     9241 aacaatggtc tgcacatgca agtgctttgg gtccttagaa gatagatata tttgcagtca
     9301 tgtagtgcgt gcggatattt atcttgtaca aaagagaggg aattgcatgt actttattgt
     9361 tttgactgac gccatgtcac aattatttaa taaacagaac atagccgcaa gtttaacaaa
     9421 cttgtggcaa tcaaattaga aaatgtgtgg cgacagaaat cggcaaacac attttagcta
     9481 tatcttaatt agtgaacaga tcgacataag ttgcaagaaa caatgacttg aaagtttaag
     9541 cgcagtcata caagttgagg acgaaagtaa atcacacaaa gcaatcttca ttagcaaaat
     9601 tctaggcaac cgattatgca tgagctaatt aagcaatgtt acttcgtagc agatctcaca
     9661 tacatacctt gtgtcttgga ggaattctcg aatcttgtcg atgatgagtt gtatatcatc
     9721 taatctagag caagcatcgt ctttcttggt gatttgtgat aatatagatc ttaatatcgc
     9781 cttcatgtca ggcttgcgtg aaatcgagac aaaagccccg cagtcgaagg tcgctccaag
     9841 gttgacccgg atctggttgg cgagcgtggt cttgcccaac cctccatatc caacaataga
     9901 gacagatttc agtgattggc cttctttctc attcagccac ttgacaagtt catcctttgg
     9961 tccatcaatt ccgacgagct ctgcggcgtc tttgtagact gcacgaagtc gagggtcgac
    10021 cttgtctttt gccggcatag aggaggagga gtcgtcaatc ttgtaccttg agtggcaaca
    10081 aatcggtcac aaatctgatg gtaggtacta atagtactgt aaggagaaca agatctggta
    10141 ccttgaaaat ctgtcgctga catccttaat ttctttcttg atgtctttga cctccttggc
    10201 gatcctgcgg cgggccttga tgtccggcag caagttcttg cacttgtcca tgagcttggc
    10261 gacgccatga gccgcctcgg aacaggagga cccgtgttcc tgttccacga ggaccatgaa
    10321 cttgtcgatg ttgtcctcta tcttatagga catctcccgc accgccgtca cacgcagctt
    10381 tgttggttcg tcaggctcct ccacatctga catcttgagg aggaacgcgc acatgacctc
    10441 cagctcgtcc ttgaggaact tgatgtcccc acggacgttc ttgagcagct tgtattcatc
    10501 cgtcaacata gctcccaact tccgcaggag ggaacccatt gcccctgtgg atgcgctcac
    10561 cagaacttcc gccatcattg agagctctct tgatcaatca gctgaaacct gcaaatcgat
    10621 gaatttaggg atctgtaaca caatccaagc tctggtggaa ctgagttggc aagccaaatc
    10681 gatgaatgtg gggatctgga ccaagcccac cttttgatgc ttcagaggag agcagaacac
    10741 aacggcggcg tcttcgtcgg atggtcacgc tgtgcggaca acgaagcggg tcagtggatt
    10801 aaacgctttg ctcgatgggg acaacacggc ggccctgtaa taaaaactta tagccgggtc
    10861 agcgccttgg atgcatggtg ctctgaataa tgactgggca aatcaatgag actcgtcaag
    10921 caggagaagc aatgactttt tagacccaag cattgctact ttactggaaa caaatcgaca
    10981 agaaacatac ccggaggttg gatgagatta gcaacaaggt ggatggatct gttcaaggca
    11041 gcacaatgga agcggggaca gaggaagtcc aacagcagtc aagagagtaa attgcttgga
    11101 atacagtggt actccgtact agatagatac agagccccac gaccccaccc tggattcaat
    11161 caacacaaat cggtcagaat ctcaagcagg agaagtaata acttttagat ccctttttag
    11221 atccaataac tggtaaaaaa acagagcaga ggaagacccc actgccacgg ccacgggtgt
    11281 caaggtcgga tcgccgtcag tggagaaaga gcagaggaag accccacctt tttgtgcttc
    11341 acggcagagc agaacacagc ggcggtggcc tcgtcggaca acgaagcgaa agcggcggct
    11401 gggcaccaga ggccagatcc gtgaggggac aagacggcgc ttcactcaat ctggaagtat
    11461 aatctgggaa atcagcagat gaacaaggaa ggtggggatg tggaagattt cagtagcaag
    11521 aaagatgaga caaagttacc aggtcagtgg attccatgcg tggtgctctg aataatgatt
    11581 ggaaactcca tgattcttgc tagctgctcc actgccaccc caaatgattc aaccaacaca
    11641 aatcgatcag actcccaagc aagcagtgaa gcaggagacg taagttttta gatccaagcg
    11701 tacttcagct ggaaagaaat atacctccac tccacaggtg tcaggggcga gctcggaggt
    11761 tggacgagct caccaaacaa gaagccggat cgaccaggag atgaggactg agatgatagg
    11821 aagggagcac tctgtcggat ccgtgagcgt gtgggcacca gaggccagat ccgtgagcgt
    11881 gtggaggtca gtggattcga tgcatggtgc aaaatagatg aatatgggga gctgtaatcc
    11941 aatcaaaact gtgtgaagct ttaatttcaa cgagctatag aacagagttg ccaatccaaa
    12001 ctgtgatatc catccaaacc cacctttgat acttcacggc agaggagaac acagccgcgg
    12061 cgtcaacacc ggatggccgt caccgttgat gcttcaccgg cagaggagga cagagcagaa
    12121 cacagcggcg tcgtagggga caagacggcg gcggatgtcg cttcactcga tctggaagaa
    12181 taagtaagaa cttaccatca agcaaatcag cagattaata agaagagaat agcaagaaag
    12241 atgggaggga aatttttacc aggccactag tatggtgctc tgaatagtca ctggaaactg
    12301 ccacccgaaa tctccacgga ggatggatga gatcagcaac aaggcggatc gatctgttcc
    12361 aggcagcgca atggaggaga ctgagaggaa gcggagacga ggagcagagg aagaccaaca
    12421 gcactcaaga gagtaaattg cttggaatat ccatcctaga tagatacaga gccccacgat
    12481 cccaccctga gttcaatcaa cacaaatgga tcagaatctg aagcaggaga agtaataagt
    12541 tttagatcca agcattgcta ctttaagtgg aaacaaatcg acaggaaata tatataccca
    12601 aaaagtccac ggtcaccaat agagcagtca gttggcaggg gcgaggccaa taggaggcga
    12661 gatgagatca gcaacaaggg cggatctatc ttattcgagg gaggaggaga ggactgagat
    12721 gagaatgagg agaggaaggg agtactcagt ggagggagac gaggagcatc aagagaggat
    12781 actactttag cacgctcctc gaatactagt acgtattaca gggagggata ccctctgcct
    12841 ttgggctgat atgtttattc agtgggtacc tatggtcagt ccaccagcca tgcttcacca
    12901 aatattatcc ctaaatagcc cttttgaaat ttaagtaagc acatcctcaa cactcttaaa
    12961 gcggccgcca ccgcggctga gtggctcctt caagcttgcg gatccgggcg gaaataggta
    13021 aagaagttgc ggataaggta attgccattg cagattattt ggattgagag tgaatatgag
    13081 actctaattg gataccgagg ggaatttatg gaacgtcagt ggagcatttt tgacaagaaa
    13141 tatttgctag ctgatagtga ccttaggcga cttttgaacg cgcaataatg gtttctgacg
    13201 tatgtgctta gctcattaaa ctccagaaac ccgcggctga gtggctcctt caacgttgcg
    13261 gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt
    13321 gactccctta attctccgct catgatcaga ttgtcgtttc ccgccttcag tttaaactat
    13381 cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata
    13441 atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc
    13501 aaccacaggg ttcccctcgg gagtgcttgg cattccgtgc gataatgact tctgttcaac
    13561 cacccaaacg tcggaaagcc tgacgacgga gcagcattcc aaaaagatcc cttggctcgt
    13621 ctgggtcggc tagaaggtcg agtgggctgc tgtggcttga tccctcaacg cggtcgcgga
    13681 cgtagcgcag cgccgaaaaa tcctcgatcg caaatccgac gctgtcgaaa agcgtgatct
    13741 gcttgtcgct ctttcggccg acgtcctggc cagtcatcac gcgccaaagt tccgtcacag
    13801 gatgatctgg cgcgagttgc tggatctcgc cttcaatccg ggtctgtggc gggaactcca
    13861 cgaaaatatc cgaacgcagc aagatcgtcg accctttccg acgctcaccg ggctggttgc
    13921 cctcgccgct gggctggcgg ccgtctatgg ccctgcaaac gcgccagaaa cgccgtcgaa
    13981 gccgtgtgcg agacaccgcg gccggccgcc ggcgttgtgg atacctcgcg gaaaacttgg
    14041 ccctcactga cagatgaggg gcggacgttg acacttgagg ggccgactca cccggcgcgg
    14101 cgttgacaga tgaggggcag gctcgatttc ggccggcgac gtggagctgg ccagcctcgc
    14161 aaatcggcga aaacgcctga ttttacgcga gtttcccaca gatgatgtgg acaagcctgg
    14221 ggataagtgc cctgcggtat tgacacttga ggggcgcgac tactgacaga tgaggggcgc
    14281 gatccttgac acttgagggg cagagtgctg acagatgagg ggcgcaccta ttgacatttg
    14341 aggggctgtc cacaggcaga aaatccagca tttgcaaggg tttccgcccg tttttcggcc
    14401 accgctaacc tgtcttttaa cctgctttta aaccaatatt tataaacctt gtttttaacc
    14461 agggctgcgc cctgtgcgcg tgaccgcgca cgccgaaggg gggtgccccc ccttctcgaa
    14521 ccctcccggc ccgctaacgc gggcctccca tccccccagg ggctgcgccc ctcggccgcg
    14581 aacggcctca ccccaaaaat ggcagccaag ctcctaacat tttattagag agcaggctag
    14641 ttgcttagat acatgatctt caggccgtta tctgtcaggg caagcgaaaa ttggccattt
    14701 atgacgacca atgccccgca gaagctccca tctttgccgc catagacgcc gcgcccccct
    14761 tttggggtgt agaacatcct tttgccagat gtggaaaaga agttcgttgt cccattgttg
    14821 gcaatgacgt agtagccggc gaaagtgcga gacccatttg cgctatatat aagcctacga
    14881 tttccgttgc gactattgtc gtaattggat gaactattat cgtagttgct ctcagagttg
    14941 tcgtaatttg atggactatt gtcgtaattg cttatggagt tgtcgtagtt gcttggagaa
    15001 atgtcgtagt tggatgggga gtagtcatag ggaagacgag cttcatccac taaaacaatt
    15061 ggcaggtcag caagtgcctg ccccgatgcc atcgcaagta cgaggcttag aaccaccttc
    15121 aacagatcgc gcatagtctt ccccagctct ctaacgcttg agttaagccg cgccgcgaag
    15181 cggcgtcggc ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct
    15241 ttcacgtagt gaacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttgt
    15301 ccaagataag cctgcctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc
    15361 attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa
    15421 atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg cggcgagttc
    15481 catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag
    15541 agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag
    15601 atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc
    15661 tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg
    15721 tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg ggaagccgaa
    15781 gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct tacggtcacc
    15841 gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc ggagccgtac
    15901 aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac tacctctgat
    15961 agttgagtcg atacttcggc gatcaccgct tccctcatga tgtttaactc ctgaattaag
    16021 ccgcgccgcg aagcggtgtc ggcttgaatg aattgttagg cgtcatcctg tgctcccgag
    16081 aaccagtacc agtacatcgc tgtttcgttc gagacttgag gtctagtttt atacgtgaac
    16141 aggtcaatgc cgccgagagt aaagccacat tttgcgtaca aattgcaggc aggtacattg
    16201 ttcgtttgtg tctctaatcg tatgccaagg agctgtctgc ttagtgccca ctttttcgca
    16261 aattcgatga gactgtgcgc gactcctttg cctcggtgcg tgtgcgacac aacaatgtgt
    16321 tcgatagagg ctagatcgtt ccatgttgag ttgagttcaa tcttcccgac aagctcttgg
    16381 tcgatgaatg cgccatagca agcagagtct tcatcagagt catcatccga gatgtaatcc
    16441 ttccggtagg ggctcacact tctggtagat agttcaaagc cttggtcgga taggtgcaca
    16501 tcgaacactt cacgaacaat gaaatggttc tcagcatcca atgtttccgc cacctgctca
    16561 gggatcaccg aaatcttcat atgacgccta acgcctggca cagcggatcg caaacctggc
    16621 gcggcttttg gcacaaaagg cgtgacaggt ttgcgaatcc gttgctgcca cttgtttaat
    16681 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
    16741 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
    16801 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    16861 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    16921 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
    16981 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    17041 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgata
    17101 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
    17161 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
    17221 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
    17281 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    17341 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    17401 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
    17461 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
    17521 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    17581 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    17641 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    17701 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
    17761 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
    17821 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
    17881 ttttctcctt acgcatctgt gcggtatttc acaccgcata tgaagatcgg cggcaatagc
    17941 ttcttagcgc catcccggct gagaaagccc agtaaggaaa caactgtagg ttcgagtcgc
    18001 gagatccccc ggaaccaaag gaagtaggtt aaacccgctc cgatcaggcc gagccacgcc
    18061 aggccgagaa cattggttcc tgtaggcatc gggattggcg gatcaaacac taaagctact
    18121 ggaacgagca gaagtcctcc ggccgccagt tgccaggcgg taaaggtgag cagaggcacg
    18181 ggaggttgcc acttgcgggt cagcacggtt ccgaacgcca tggaaaccgc ccccgccagg
    18241 cccgctgcga cgccgacagg atctagcgct gcgtttggtg tcaacaccaa cagcgccacg
    18301 cccgcagttc cgcaaatagc ccccaggacc gccatcaatc gtatcgggct acctagcaga
    18361 gcggcagaga tgaacacgac catcagcggc tgcacagcgc ctaccgtcgc cgcgaccccg
    18421 cccggcaggc ggtagaccga aataaacaac aagctccaga atagcgaaat attaagtgcg
    18481 ccgaggatga agatgcgcat ccaccagatt cccgttggaa tctgtcggac gatcatcacg
    18541 agcaataaac ccgccggcaa cgcccgcagc agcataccgg cgacccctcg gcctcgctgt
    18601 tcgggctcca cgaaaacgcc ggacagatgc gccttgtgag cgtccttggg gccgtcctcc
    18661 tgtttgaaga ccgacagccc aatgatctcg ccgtcgatgt aggcgccgaa tgccacggca
    18721 tctcgcaacc gttcagcgaa cgcctccatg ggctttttct cctcgtgctc gtaaacggac
    18781 ccgaacatct ctggagcttt cttcagggcc gacaatcgga tctcgcggaa atcctgcacg
    18841 tcggccgctc caagccgtcg aatctgagcc ttaatcacaa ttgtcaattt taatcctctg
    18901 tttatcggca gttcgtagag cgcgccgtgc gtcccgagcg atactgagcg aagcaagtgc
    18961 gtcgagcagt gcccgcttgt tcctgaaatg ccagtaaagc gctggctgct gaacccccag
    19021 ccggaactga ccccacaagg ccctagcgtt tgcaatgcac caggtcatca ttgacccagg
    19081 cgtgttccac caggccgctg cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc
    19141 acttcttcac gcgggtggaa tccgatccgc acatgaggcg gaaggtttcc agcttgagcg
    19201 ggtacggctc ccggtgcgag ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct
    19261 tgcggtactt ctcccatatg aatttcgtgt agtggtcgcc agcaaacagc acgacgattt
    19321 cctcgtcgat caggacctgg caacgggacg ttttcttgcc acggtccagg acgcggaagc
    19381 ggtgcagcag cgacaccgat tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg
    19441 tcgcctgtag gcgcgacagg cattcctcgg ccttcgtgta ataccggcca ttgatcgacc
    19501 agcccaggtc ctggcaaagc tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc
    19561 gcttcgcgta ctccaacacc tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga
    19621 cgccggtgta ggtgatcttc acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg
    19681 cctcgcgcgg gattttcttg ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg
    19741 gcatcgctcg catcgtgtcc ggccacggcg caatatcgaa caaggaaagc tgcatttcct
    19801 tgatctgctg cttcgtgtgt ttcagcaacg cggcctgctt ggcctcgctg acctgttttg
    19861 ccaggtcctc gccggcggtt tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg
    19921 tcatcgactt cgccaaacct gccgcctcct gttcgagacg acgcgaacgc tccacggcgg
    19981 ccgatggcgc gggcagggca gggggagcca gttgcacgct gtcgcgctcg atcttggccg
    20041 tagcttgctg gaccatcgag ccgacggact ggaaggtttc gcggggcgca cgcatgacgg
    20101 tgcggcttgc gatggtttcg gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc
    20161 tgtatgcctt ccggtcaaac gtccgattca ttcaccctcc ttgcgggatt gccccgactc
    20221 acgccggggc aatgtgccct tattcctgat ttgacccgcc tggtgccttg gtgtccagat
    20281 aatccacctt atcggcaatg aagtcggtcc cgtagaccgt ctggccgtcc ttctcgtact
    20341 tggtattccg aatcttgccc tgcacgaata ccagctccgc gaagtcgctc ttcttgatgg
    20401 agcgcatggg gacgtgcttg gcaatcacgc gcaccccccg gccgttttag cggctaaaaa
    20461 agtcatggct ctgccctcgg gcggaccacg cccatcatga ccttgccaag ctcgtcctgc
    20521 ttctcttcga tcttcgccag cagggcgagg atcgtggcat caccgaaccg cgccgtgcgc
    20581 gggtcgtcgg tgagccagag tttcagcagg ccgcccaggc ggcccaggtc gccattgatg
    20641 cgggccagct cgcggacgtg ctcatagtcc acgacgcccg tgattttgta gccctggccg
    20701 acggccagca ggtaggccga caggctcatg ccggccgccg ccgccttttc ctcaatcgct
    20761 cttcgttcgt ctggaaggca gtacaccttg ataggtgggc tgcccttcct ggttgggtaa
    20821 tgactccaac ttattgatag tgttttatgt tcagataatg cccgatgact ttgtcatgca
    20881 gctccaccga ttttgagaac gacagcgact tccgtcccag ccgtgccagg tgctgcctca
    20941 gattcaggtt atgccgctca attcgctgcg tatatcgctt gctgattacg tgcagctttc
    21001 ccttcaggcg ggattcatac agcggccagc catccgtcat ccatatcacc acgtcaaagg
    21061 gtgacagcag gctcataaga cgccccagcg tcgccatagt gcgttcaccg aatacgtgcg
    21121 caacaaccgt cttccggaga ctgtcatacg cgtaaaacag ccagcgctgg cgcgatttag
    21181 ccccgacata gccccactgt tcgtccattt ccgcgcagac gatgacgtca ctgcccggct
    21241 gtatgcgcga ggttaccgac tgcggcctga gttttttaag tgacgtaaaa tcgtgttgag
    21301 gccaacgccc ataatgcggg ctgttgcccg gcatccaacg ccattcatgg ccatatcaat
    21361 gattttctgg tgcgtaccgg gttgagaagc ggtgtaagtg aactgcagtt gccatgtttt
    21421 acggcagtga gagcagagat agcgctgatg tccggcggtg cttttgccgt tacgcaccac
    21481 cccgtcagta gctgaacagg agggacagct gatagaaaca gaagccactg gagcacctca
    21541 aaaacaccat catacactaa atcagtaagt tggcagcatc acccctggtt ggcttggttt
    21601 catcagccat ccgcttgccc tcatctgtta cgccggcggt agccggccag cctcgcagag
    21661 caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag gaacacccgc
    21721 tcgcgggtgg gcctacttca cctatcctgc ccggctgacg ccgttggata caccaaggaa
    21781 agtctacacg aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat ggatataccg
    21841 aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc ggaaaagatc c
//
