LOCUS       sequence-200186-        9373 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting
            LMNA. TagBFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9373)
  AUTHORS   Arnoult N, Correia A, Ma J, Merlo A, Garcia-Gomez S, Maric M, 
            Tognetti M, Benner CW, Boulton SJ, Saghatelian A, Karlseder J
  TITLE     Regulation of DNA repair pathway choice in S and G2 phases by the 
            NHEJ inhibitor CYREN.
  JOURNAL   Nature. 2017 Sep 20;549(7673):548-552. doi: 10.1038/nature24023.
  PUBMED    28959974
REFERENCE   2  (bases 1 to 9373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2017-09-20- 20"; volume: "549"; issue: "7673"; 
            pages: "548-552"
COMMENT     Sequence Label: pUC CBA-SpCas9.EF1a-BFP.sgLMNA
FEATURES             Location/Qualifiers
     source          1..9373
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             258..323
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             330..350
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             375..4475
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             375..4475
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4476..4523
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(4551..4568)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4557..4764
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4773..4913
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4773..4902
                     /label=AAV2 ITR
     rep_origin      4988..5443
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5075..5094)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5285..5306
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5460..5479)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5579..5601
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5639..5657)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5725..5829
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5830..5898,5899..6690)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6048..6067)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6861..7449
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7350..7369
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7511..7759
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7511..7531
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7682..7701
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        7780..7855
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        7952..8163
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             8226..8927
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     enhancer        8966..9251
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga
       61 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
      121 tagctgagca agaggtaagg gtttaaggga tggttggttg gtggggtatt aatgtttaat
      181 tacctggagc acctgcctga aatcactttt tttcaggttg gaccgggagt ttggcaacat
      241 atgccatatg ctggatggac tataaggacc acgacggaga ctacaaggat catgatattg
      301 attacaaaga cgatgacgat aagatggccc caaagaagaa gcggaaggtc ggtatccacg
      361 gagtcccagc agccgacaag aagtacagca tcggcctgga catcggcacc aactctgtgg
      421 gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca
      481 acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg
      541 aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga
      601 accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct
      661 tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc
      721 ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc
      781 acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg
      841 ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg
      901 acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg
      961 aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga
     1021 gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc
     1081 tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg
     1141 acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca
     1201 acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt
     1261 ccgacgccat cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc
     1321 tgagcgcctc tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag
     1381 ctctcgtgcg gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga
     1441 acggctacgc cggctacatt gacggcggag ccagccagga agagttctac aagttcatca
     1501 agcccatcct ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg
     1561 acctgctgcg gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg
     1621 gagagctgca cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc
     1681 gggaaaagat cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca
     1741 ggggaaacag cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga
     1801 acttcgagga agtggtggac aagggcgctt ccgcccagag cttcatcgag cggatgacca
     1861 acttcgataa gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt
     1921 acttcaccgt gtataacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc
     1981 ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc
     2041 ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact
     2101 ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc
     2161 tgctgaaaat tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg
     2221 aagatatcgt gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga
     2281 aaacctatgc ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca
     2341 ccggctgggg caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca
     2401 agacaatcct ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga
     2461 tccacgacga cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg
     2521 gcgatagcct gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca
     2581 tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg
     2641 agaacatcgt gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca
     2701 gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga
     2761 aagaacaccc cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc
     2821 agaatgggcg ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg
     2881 atgtggacca tatcgtgcct cagagctttc tgaaggacga ctccatcgac aacaaggtgc
     2941 tgaccagaag cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga
     3001 agaagatgaa gaactactgg cggcagctgc tgaacgccaa gctgattacc cagagaaagt
     3061 tcgacaatct gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca
     3121 tcaagagaca gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact
     3181 cccggatgaa cactaagtac gacgagaatg acaagctgat ccgggaagtg aaagtgatca
     3241 ccctgaagtc caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg
     3301 agatcaacaa ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc
     3361 tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg
     3421 acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact
     3481 tcttctacag caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga
     3541 tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg
     3601 gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa
     3661 agaccgaggt gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg
     3721 ataagctgat cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc
     3781 ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac
     3841 tgaagagtgt gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga
     3901 atcccatcga ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca
     3961 agctgcctaa gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg
     4021 ccggcgaact gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt
     4081 acctggccag ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc
     4141 tgtttgtgga acagcacaag cactacctgg acgagatcat cgagcagatc agcgagttct
     4201 ccaagagagt gatcctggcc gacgctaatc tggacaaagt gctgtccgcc tacaacaagc
     4261 accgggataa gcccatcaga gagcaggccg agaatatcat ccacctgttt accctgacca
     4321 atctgggagc ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca
     4381 ccagcaccaa agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg
     4441 agacacggat cgacctgtct cagctgggag gcgacaaaag gccggcggcc acgaaaaagg
     4501 ccggccaggc aaaaaagaaa aagtaagaat tcctagagct cgctgatcag cctcgactgt
     4561 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     4621 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     4681 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     4741 agagaatagc aggcatgctg gggagcggcc gcaggaaccc ctagtgatgg agttggccac
     4801 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
     4861 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct
     4921 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
     4981 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5041 gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt
     5101 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     5161 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     5221 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     5281 aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt
     5341 gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa
     5401 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
     5461 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     5521 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     5581 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     5641 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     5701 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5761 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5821 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5881 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5941 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     6001 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     6061 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     6121 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     6181 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     6241 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     6301 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     6361 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     6421 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     6481 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6541 cgtggaagcc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     6601 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6661 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6721 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6781 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6841 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6901 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6961 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     7021 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7081 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7141 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7201 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7261 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7321 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7381 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7441 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7501 gctcacatgt gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc
     7561 tgttagagag ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac
     7621 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat
     7681 ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt
     7741 gtggaaagga cgaaacaccg gttggcagcg ctgcccgcgg ttttagagct agaaatagca
     7801 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
     7861 tgttttagag ctagaaatag caagttaaaa taaggctagt ccgtttttag cgcgtgcgcc
     7921 aattctgcag acatgaatca acctctggat tgggcagagc gcacatcgcc cacagtcccc
     7981 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
     8041 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     8101 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     8161 cagctctggc taactagaga acccactgta gtgaaccgtc agatcgcctg gagacgccat
     8221 ccacgatgtc cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
     8281 tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
     8341 agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
     8401 ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact
     8461 tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg
     8521 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
     8581 tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg
     8641 gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg
     8701 acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacatcaag accacatata
     8761 gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac
     8821 tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg
     8881 ccagatactg cgacctccct agcaaactgg ggcacaagct taattgaatc tcatgctgga
     8941 gttgccctgg ctcacaaata ccacccgtta cataacttac ggtaaatggc ccgcctggct
     9001 gaccgcccaa cgacccccgc ccattgacgt caatagtaac gccaataggg actttccatt
     9061 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     9121 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattgtg
     9181 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     9241 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
     9301 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
     9361 gggggggggg ggg
//
