LOCUS       sequence-200187-       10621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mutant BAF (L58R) in human cells (with EGFP produced from 
            the same transcript as expression control).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10621)
  AUTHORS   Samwer M, Schneider MWG, Hoefler R, Schmalhorst PS, Jude JG, Zuber 
            J, Gerlich DW
  TITLE     DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic 
            Chromosomes.
  JOURNAL   Cell. 2017 Aug 24;170(5):956-972.e23. doi: 
            10.1016/j.cell.2017.07.038.
  PUBMED    28841419
REFERENCE   2  (bases 1 to 10621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.07"; journalName: "Cell"; date: "2017-08-24- 
            24"; volume: "170"; issue: "5"; pages: "956-972.e23"
COMMENT     Sequence Label: EGFP-P2A_BAF_L58R
FEATURES             Location/Qualifiers
     source          1..10621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1679..1912
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2097..2141
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2439..2556
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2649..2860
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2875..2884
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2881..2883,2884..2886,2887..3597)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2881..2883,2884..2886,2887..3597)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2926..2947)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3187..3206)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3534..3555
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3607..3663
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     intron          4003..4232
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4215..4234
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    4290..4861
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4455..4472)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4682..4701
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4894..5292
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    5312..5900
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5365..5385)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5783..5794)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5903..5919)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5904..5920)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6425..6605
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6631..6648)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6637..6861
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6907..7335
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6994..7013)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7204..7225
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7344..7364)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7349..7678
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7529..7664
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7591..7610
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7726..7773
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7792..8166
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8296..8417
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8333..8352)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8387..8406
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8466..8482)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8466..8482)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8479..8501)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8490..8506
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8514..8520,8521..8538,8539..8544))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8559..8580
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8697..8714)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8868..9456)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8948..8967)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9627..10418,10419..10487))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10250..10269
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10488..10592)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     1561 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1621 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1681 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1741 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1801 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1861 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1921 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1981 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     2041 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     2101 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2161 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2221 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2281 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     2341 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     2401 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     2461 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2521 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     2581 gatccagttt ggttaattag ctagctaggt cttgaaagga gtgggaattg gctccggtgc
     2641 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     2701 caattgatcc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     2761 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     2821 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gagaattctc tagcgccacc
     2881 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     2941 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctat
     3001 ggtaagctta ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     3061 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     3121 cagcacgact ttttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     3181 tttaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     3241 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     3301 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     3361 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     3421 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     3481 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     3541 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggga
     3601 tccggcgcaa caaacttctc tctgctgaaa caagccggag atgtcgaaga gaatcctgga
     3661 ccgaccggta tgacaacctc ccaaaagcac cgagacttcg tggcagagcc catgggggag
     3721 aagccagtgg ggagcctggc tgggattggt gaagtcctgg gcaagaagct ggaggaaagg
     3781 ggttttgaca aggcctatgt tgtccttggc cagtttctgg tgctaaagaa agatgaagac
     3841 cgcttccggg aatggctgaa agacacttgt ggcgccaacg ccaagcagtc ccgggactgc
     3901 ttcggatgcc ttcgagagtg gtgtgatgcc ttcttgtagg gatccgaatt ccgatccagt
     3961 gtgctgcaat taattcgctg tctgcgaggg ccagctgttg gggtgagtac tccctctcaa
     4021 aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat
     4081 tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca gaaaagacaa
     4141 tctttttgtt gtcaagcttg aggtgtggca ggcttgagat ctggccatac acttgagtga
     4201 caatgacatc cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcaggt
     4261 cgagcatgca tctagggcgg ccaattccgc ccctctcccc ccccccccta acgttactgg
     4321 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     4381 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     4441 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     4501 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     4561 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     4621 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     4681 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     4741 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     4801 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     4861 agcttgccac aacccacaag gagacgacct tccatggcca agcctttgtc tcaagaagaa
     4921 tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac
     4981 agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat
     5041 cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca
     5101 gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc
     5161 tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag
     5221 gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg
     5281 tgggagggct aacgatatca agcttatcgg taatcaacct ctggattaca aaatttgtga
     5341 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     5401 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     5461 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     5521 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     5581 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     5641 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     5701 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     5761 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     5821 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     5881 cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc
     5941 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
     6001 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
     6061 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
     6121 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
     6181 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
     6241 ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac
     6301 ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga
     6361 gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg
     6421 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     6481 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     6541 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     6601 tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     6661 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     6721 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     6781 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     6841 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     6901 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     6961 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     7021 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     7081 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     7141 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     7201 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     7261 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     7321 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     7381 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     7441 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7501 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     7561 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     7621 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     7681 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
     7741 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
     7801 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
     7861 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
     7921 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
     7981 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
     8041 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
     8101 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
     8161 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     8221 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     8281 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     8341 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     8401 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     8461 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     8521 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     8581 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     8641 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     8701 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     8761 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     8821 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8881 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     8941 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     9001 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     9061 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9121 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9181 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9241 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     9301 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     9361 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     9421 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     9481 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     9541 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     9601 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     9661 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     9721 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9781 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     9841 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9901 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     9961 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    10021 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    10081 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    10141 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    10201 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    10261 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    10321 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    10381 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    10441 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    10501 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    10561 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    10621 g
//
