LOCUS       sequence-200199-        7179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus rescue vector, VSVGED pseudotype, mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Oliveira CR, Lemaitre R, Murawala P, Tazaki A, Drechsel DN, Tanaka 
            EM
  TITLE     Pseudotyped baculovirus is an effective gene expression tool for 
            studying molecular function during axolotl limb regeneration.
  JOURNAL   Dev Biol. 2018 Jan 15;433(2):262-275. doi: 
            10.1016/j.ydbio.2017.10.008. Epub 2017 Nov 30.
  PUBMED    29198566
REFERENCE   2  (bases 1 to 7179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2017.10.008"; journalName: "Dev Biol"; date: 
            "2018-01-15- 15"; volume: "433"; issue: "2"; pages: "262-275"
COMMENT     Sequence Label: pOEM-GED-mCherry
FEATURES             Location/Qualifiers
     source          1..7179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      10..598
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     499..518
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     691..708
                     /label=L4440
                     /note="L4440 vector, forward primer"
     3'UTR           1060..1432
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     enhancer        1533..1836
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1837..2040
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1991..2011
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2079..2088
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2085..2795
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2230..2248)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2498..2517
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(2876..2896)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3294..3305)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3540..3674
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3577..3596)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3631..3650
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(3787..3819)
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     sig_peptide     complement(4015..4074)
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     promoter        complement(4137..4228)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(4195..4212)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4314..4919
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(4957..5081)
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     primer_bind     complement(5691..5710)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5692..5710)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5721..5738)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5721..5737)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5730..5752)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        6053..6157
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6158..6226,6227..7018)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6376..6395)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
       61 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      121 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      181 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      241 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      301 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      361 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      421 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      481 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      541 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      601 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcattaggc
      661 accccaggct ttacccgaac gaccgagcgc agcgagtcag tgagcgagga agcggagagc
      721 gcccaatacg caaggaaaca gctatgacca tgttaatgca gctggcacga caggtttccc
      781 gactggaaag cgggcagtga gcggaaggcc catgaggccc aaaaagctca actacaaatt
      841 tgatcgcgga cgtgttagcc gacacaatta ataggcgtcg tgtggctatg gcaaaatcgt
      901 cttcggaagc aacttctaac gacgagggtt gggacgacga cgataatcgg cctaataaag
      961 ctaacacgcc cgatgttaaa tatgtccaag cgttatttaa cgtgtttacg tcgagtcaat
     1021 tgtacactaa cgacagtgat gaaagaaata caaaagcgca taatattttg aacgacgtcg
     1081 aacctttatt acaaaacaaa acacaaacga atatcgacaa agctagattg ctgctacaag
     1141 atttggcaag ttttgtggcg ttgagcgaaa atccattaga tagtccagcc atcggttcgg
     1201 aaaaacaacc cttgtttgaa actaatcgaa acctatttta caaatctatt gaggatttaa
     1261 tatttaaatt cagatataaa gacgctgaaa atcatttgat tttcgctcta acataccacc
     1321 ctaaagatta taaatttaat gaattattaa aatacgtaca acaattgtct gtaaatcaac
     1381 aacgcacaga atctagcgct taataaatgt actaataaca atgtatcgtg ttttattaat
     1441 taagatctgc ggatgcaggc tccgccatgc attagttatt aatagtaatc aattacgggg
     1501 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     1561 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     1621 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     1681 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     1741 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     1801 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     1861 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     1921 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     1981 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     2041 ggtttagtga accgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg
     2101 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     2161 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     2221 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     2281 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     2341 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     2401 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     2461 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     2521 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
     2581 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
     2641 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
     2701 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
     2761 actccaccgg cggcatggac gagctgtaca agtaagaatt cgcggccgct agacgcgtcg
     2821 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     2881 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     2941 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     3001 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     3061 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     3121 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     3181 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     3241 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     3301 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     3361 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg aagcttgtcg
     3421 agaagtacta gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     3481 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     3541 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     3601 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     3661 atcatgtctg gatctgatca ctggcgcgcc gtgatgcgta tttctgaatc tttgtaaaat
     3721 agcacacaag actccaacgc gtttggcgtt ttattttctt gctcgactct agtttattag
     3781 gccttacttt ccaagtcggt tcatctctat gtctgtataa atctgtcttt tcttggtgtg
     3841 ctttaattta atgcaaagat ggataccaac tcggagaacc aagaatagtc caatgattaa
     3901 ccctatgata aagaaaaaag aggcaataga gcttttccaa ctactgaacc aaccttctac
     3961 aagctcgatt ggatttttgg atagcccagt atcaccccgg ggtacctgca gatccgcaaa
     4021 ggcagaatgc gccgccgccg ccaaaagcac atataaaaca atagcgctta ccatcttgct
     4081 tgtgtgttcc ttattgaagc cttggtgtga ctgatttact agtagcatag atccgtattt
     4141 ataggttttt ttattacaaa actgttacga aaacagtaaa atacttattt atttgcgaga
     4201 tggttatcat tttaattatc tccatgatcc agccaaccac tagaactata gctagagtcc
     4261 tgggcgaaca aaatggtggc agcttgtgat attagtttgt gcgtctcatt acaatggctg
     4321 ttatttttaa caacaaacaa ctgctcgcag acaatagtat agaaaaggga ggtgaactgt
     4381 ttttgtttaa cggttcgtac aacattttgg aaagttatgt taatccggtg ctgctaaaaa
     4441 atggtgtaat tgaactagaa gaagctgcgt actatgccgg caacatattg tacaaaaccg
     4501 acgatcccaa attcattgat tatataaatt taataattaa agcaacacac tccgaagaac
     4561 taccagaaaa tagcactgtt gtaaattaca gaaaaactat gcgcagcggt actatacacc
     4621 ccattaaaaa agacatatat atttatgaca acaaaaaatt tactctatac gatagataca
     4681 tatatggata cgataataac tatgttaatt tttatgagga gaaaaatgaa aaagagaagg
     4741 aatacgaaga agaagacgac aaggcgtcta gtttatgtga aaataaaatt atattgtcgc
     4801 aaattaactg tgaatcattt gaaaatgatt ttaaatatta cctcagcgat tataactacg
     4861 cgttttcaat tatagataat actacaaatg ttcttgttgc gtttggtttg tatcgttaat
     4921 aaaaaacaaa tttagcattt ataattgttt tattattcaa taattacaaa taggattgag
     4981 acccttgcag ttgccagcaa acggacagag cttgtccaca gttttcattt tttggcagtt
     5041 tggtggttta tacacatctt gggatttgat taaaagatgc atgacttcac ccacacactt
     5101 ggagtcgtgg ttgtagaaat gcgtcagagc cttgattaaa catcggtttt cacacttggc
     5161 acacgaaacc acgtttgcaa tgtaacagtt tagtataaac ctcttcctgt acataccgcc
     5221 gcgcggtaca ttgttttctt tcaaatcgtt caatattttt tttatacacg gaggcatatt
     5281 aatcttgttg ttcagcatgt ccacgacatt tttctttcct tctacgcatt ctttcataca
     5341 aatgtttctc ttgctgcgtt ttttaaaatt ggaattttta atttcattaa tagtgggcgg
     5401 agccgttaac agcatgtaca atcgcagtcc ggaaattttg accaaaaccc cgccattgta
     5461 gaataccgtg tatggagtta gggtcaattt atcaataaaa tcatcgggat caattaaata
     5521 tgtagctttc ttgtctaaac atgacgctct aatgagcgga ctccacacgt tatacgatgc
     5581 attcgccata cttcgcggct tctcgcacca gcggtcctga ttcgactaac ggtcgattgg
     5641 atggcttaat agttttaacg tcacggcctt gacggccttc cgccaattcg ccctatagtg
     5701 agtcgtatta cgtcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     5761 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     5821 gaagaggccc gcaccgaaac gcccttccca acagttgcgc agcctgaatg gcgaatggga
     5881 gcgccctgta gcggccactc aaccctatct cggtctattc ttttgattta taagggattt
     5941 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     6001 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     6061 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     6121 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     6181 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     6241 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     6301 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     6361 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     6421 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     6481 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     6541 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6601 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     6661 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     6721 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6781 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6841 tattgctgat aaatctggag ccggtgagcg tggttctcgc ggtatcattg cagcactggg
     6901 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6961 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     7021 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     7081 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7141 ttcgttccac tgagcgtcag accccgtaga aaagatcaa
//
