LOCUS       sequence-200200-        7180 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus rescue vector, VSVGED pseudotype, eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7180)
  AUTHORS   Oliveira CR, Lemaitre R, Murawala P, Tazaki A, Drechsel DN, Tanaka 
            EM
  TITLE     Pseudotyped baculovirus is an effective gene expression tool for 
            studying molecular function during axolotl limb regeneration.
  JOURNAL   Dev Biol. 2018 Jan 15;433(2):262-275. doi: 
            10.1016/j.ydbio.2017.10.008. Epub 2017 Nov 30.
  PUBMED    29198566
REFERENCE   2  (bases 1 to 7180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2017.10.008"; journalName: "Dev Biol"; date: 
            "2018-01-15- 15"; volume: "433"; issue: "2"; pages: "262-275"
COMMENT     Sequence Label: pOEM-GED-EGFP
FEATURES             Location/Qualifiers
     source          1..7180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      14..602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     503..522
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     695..712
                     /label=L4440
                     /note="L4440 vector, forward primer"
     3'UTR           1064..1436
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     enhancer        1537..1840
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1841..2044
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1995..2015
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2083..2092
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2089..2091,2092..2094,2095..2805)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2089..2091,2092..2094,2095..2805)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2134..2155)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2395..2414)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2742..2763
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2881..2901)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3299..3310)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3545..3679
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3582..3601)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3636..3655
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(3792..3824)
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     sig_peptide     complement(4020..4079)
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     promoter        complement(4142..4233)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(4200..4217)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4319..4924
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(4962..5086)
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     primer_bind     complement(5696..5715)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5697..5715)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5726..5743)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5726..5742)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5735..5757)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        6058..6162
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6163..6231,6232..7023)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6381..6400)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
       61 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      121 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      181 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      241 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      301 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      361 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      421 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      481 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      541 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      601 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcatt
      661 aggcacccca ggctttaccc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      721 gagcgcccaa tacgcaagga aacagctatg accatgttaa tgcagctggc acgacaggtt
      781 tcccgactgg aaagcgggca gtgagcggaa ggcccatgag gcccaaaaag ctcaactaca
      841 aatttgatcg cggacgtgtt agccgacaca attaataggc gtcgtgtggc tatggcaaaa
      901 tcgtcttcgg aagcaacttc taacgacgag ggttgggacg acgacgataa tcggcctaat
      961 aaagctaaca cgcccgatgt taaatatgtc caagcgttat ttaacgtgtt tacgtcgagt
     1021 caattgtaca ctaacgacag tgatgaaaga aatacaaaag cgcataatat tttgaacgac
     1081 gtcgaacctt tattacaaaa caaaacacaa acgaatatcg acaaagctag attgctgcta
     1141 caagatttgg caagttttgt ggcgttgagc gaaaatccat tagatagtcc agccatcggt
     1201 tcggaaaaac aacccttgtt tgaaactaat cgaaacctat tttacaaatc tattgaggat
     1261 ttaatattta aattcagata taaagacgct gaaaatcatt tgattttcgc tctaacatac
     1321 caccctaaag attataaatt taatgaatta ttaaaatacg tacaacaatt gtctgtaaat
     1381 caacaacgca cagaatctag cgcttaataa atgtactaat aacaatgtat cgtgttttat
     1441 taattaagat ctgcggatgc aggctccgcc atgcattagt tattaatagt aatcaattac
     1501 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     1561 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     1621 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     1681 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     1741 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     1801 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     1861 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     1921 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     1981 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     2041 agctggttta gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgagcaag
     2101 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     2161 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     2221 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     2281 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     2341 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     2401 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     2461 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     2521 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     2581 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     2641 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     2701 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     2761 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtgcgg ccgctagacg
     2821 cgtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     2881 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     2941 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     3001 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     3061 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     3121 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     3181 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     3241 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     3301 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     3361 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgaagct
     3421 tgtcgagaag tactagagga tcataatcag ccataccaca tttgtagagg ttttacttgc
     3481 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     3541 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     3601 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     3661 atcttatcat gtctggatct gatcactggc gcgccgtgat gcgtatttct gaatctttgt
     3721 aaaatagcac acaagactcc aacgcgtttg gcgttttatt ttcttgctcg actctagttt
     3781 attaggcctt actttccaag tcggttcatc tctatgtctg tataaatctg tcttttcttg
     3841 gtgtgcttta atttaatgca aagatggata ccaactcgga gaaccaagaa tagtccaatg
     3901 attaacccta tgataaagaa aaaagaggca atagagcttt tccaactact gaaccaacct
     3961 tctacaagct cgattggatt tttggatagc ccagtatcac cccggggtac ctgcagatcc
     4021 gcaaaggcag aatgcgccgc cgccgccaaa agcacatata aaacaatagc gcttaccatc
     4081 ttgcttgtgt gttccttatt gaagccttgg tgtgactgat ttactagtag catagatccg
     4141 tatttatagg tttttttatt acaaaactgt tacgaaaaca gtaaaatact tatttatttg
     4201 cgagatggtt atcattttaa ttatctccat gatccagcca accactagaa ctatagctag
     4261 agtcctgggc gaacaaaatg gtggcagctt gtgatattag tttgtgcgtc tcattacaat
     4321 ggctgttatt tttaacaaca aacaactgct cgcagacaat agtatagaaa agggaggtga
     4381 actgtttttg tttaacggtt cgtacaacat tttggaaagt tatgttaatc cggtgctgct
     4441 aaaaaatggt gtaattgaac tagaagaagc tgcgtactat gccggcaaca tattgtacaa
     4501 aaccgacgat cccaaattca ttgattatat aaatttaata attaaagcaa cacactccga
     4561 agaactacca gaaaatagca ctgttgtaaa ttacagaaaa actatgcgca gcggtactat
     4621 acaccccatt aaaaaagaca tatatattta tgacaacaaa aaatttactc tatacgatag
     4681 atacatatat ggatacgata ataactatgt taatttttat gaggagaaaa atgaaaaaga
     4741 gaaggaatac gaagaagaag acgacaaggc gtctagttta tgtgaaaata aaattatatt
     4801 gtcgcaaatt aactgtgaat catttgaaaa tgattttaaa tattacctca gcgattataa
     4861 ctacgcgttt tcaattatag ataatactac aaatgttctt gttgcgtttg gtttgtatcg
     4921 ttaataaaaa acaaatttag catttataat tgttttatta ttcaataatt acaaatagga
     4981 ttgagaccct tgcagttgcc agcaaacgga cagagcttgt ccacagtttt cattttttgg
     5041 cagtttggtg gtttatacac atcttgggat ttgattaaaa gatgcatgac ttcacccaca
     5101 cacttggagt cgtggttgta gaaatgcgtc agagccttga ttaaacatcg gttttcacac
     5161 ttggcacacg aaaccacgtt tgcaatgtaa cagtttagta taaacctctt cctgtacata
     5221 ccgccgcgcg gtacattgtt ttctttcaaa tcgttcaata ttttttttat acacggaggc
     5281 atattaatct tgttgttcag catgtccacg acatttttct ttccttctac gcattctttc
     5341 atacaaatgt ttctcttgct gcgtttttta aaattggaat ttttaatttc attaatagtg
     5401 ggcggagccg ttaacagcat gtacaatcgc agtccggaaa ttttgaccaa aaccccgcca
     5461 ttgtagaata ccgtgtatgg agttagggtc aatttatcaa taaaatcatc gggatcaatt
     5521 aaatatgtag ctttcttgtc taaacatgac gctctaatga gcggactcca cacgttatac
     5581 gatgcattcg ccatacttcg cggcttctcg caccagcggt cctgattcga ctaacggtcg
     5641 attggatggc ttaatagttt taacgtcacg gccttgacgg ccttccgcca attcgcccta
     5701 tagtgagtcg tattacgtcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     5761 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     5821 atagcgaaga ggcccgcacc gaaacgccct tcccaacagt tgcgcagcct gaatggcgaa
     5881 tgggagcgcc ctgtagcggc cactcaaccc tatctcggtc tattcttttg atttataagg
     5941 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     6001 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     6061 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     6121 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     6181 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     6241 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     6301 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     6361 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     6421 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     6481 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     6541 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     6601 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     6661 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     6721 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6781 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6841 tggtttattg ctgataaatc tggagccggt gagcgtggtt ctcgcggtat cattgcagca
     6901 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6961 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     7021 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     7081 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     7141 gagttttcgt tccactgagc gtcagacccc gtagaaaaga
//
