LOCUS       sequence-200203-         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mutant human alpha synuclein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Rospigliosi CC, McClendon S, Schmid AW, Ramlall TF, Barre P, Lashuel
            HA, Eliezer D
  TITLE     E46K Parkinson's-linked mutation enhances C-terminal-to-N-terminal 
            contacts in alpha-synuclein.
  JOURNAL   J Mol Biol. 2009 May 22;388(5):1022-32. doi: 
            10.1016/j.jmb.2009.03.065. Epub 2009 Apr 5.
  PUBMED    19345692
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2009.03.065"; journalName: "J Mol Biol"; date: 
            "2009-05-22- 22"; volume: "388"; issue: "5"; pages: "1022-32"
COMMENT     Sequence Label: pT7-7 asyn A53T
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttgtttaact ttaagaagga gatatacata tggatgtatt catgaaagga ctttcaaagg
       61 ccaaggaggg agttgtggct gctgctgaga aaaccaaaca gggtgtggca gaagcagcag
      121 gaaagacaaa agagggtgtt ctctatgtag gctccaaaac caaggaggga gtggtgcatg
      181 gtgtgacaac agtggctgag aagaccaaag agcaagtgac aaatgttgga ggagcagtgg
      241 tgacgggtgt gacagcagta gcccagaaga cagtggaggg agcagggagc attgcagcag
      301 ccactggctt tgtcaaaaag gaccagttgg gcaagaatga agaaggagcc ccacaggaag
      361 gaattctgga agatatgcct gtggatcctg acaatgaggc ttatgaaatg ccttctgagg
      421 aagggtatca agactacgaa cctgaagcct aagaaatatc tttgctccca gtttcttgag
      481 atctgctgac agatgttcca tccaagctta tcgatgataa gctgtcaaac atgagaatta
      541 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      601 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccnntc
      661 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
      721 cgagacc
//
