LOCUS       sequence-200209-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mutant human alpha synuclein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  TITLE     Lashuel Lab plasmids
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET11a asyn Q79N (TAC-->TAT)
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             459..491
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     primer_bind     complement(569..587)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      583..630
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(805..810,811..827,828..833))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(860..878)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatggatgt attcatgaaa ggactttcaa
       61 aggccaagga gggagttgtg gctgctgctg agaaaaccaa acagggtgtg gcagaagcag
      121 caggaaagac aaaagagggt gttctctatg taggctccaa aaccaaggag ggagtggtgc
      181 atggtgtggc aacagtggct gagaagacca aagagcaagt gacaaatgtt ggaggagcag
      241 tggtgacggg tgtgacagca gtagccaaca agacagtgga gggagcgggg agcattgcag
      301 cagccactgg ctttgtcaaa aaggaccagt tgggcaagaa tgaagaagga gccccacagg
      361 aaggaattct ggaagatatg cctgtggatc ctgacaatga ggcttatgaa atgccttctg
      421 aggaagggta tcaagactat gaacctgaag cctaacatat ggctagcatg actggtggac
      481 agcaaatggg tcgcggatcc cgggtacctc gagtcgaccg atccggctgc taacaaagcc
      541 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
      601 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatccc
      661 gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg
      721 tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc
      781 aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag
      841 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
      901 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      961 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1021 atgcttcaat
//
