LOCUS       sequence-200211-        1072 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mutant human alpha synuclein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1072)
  TITLE     Lashuel Lab plasmids
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET11a asyn Q109E (TAC-->TAT)
FEATURES             Location/Qualifiers
     source          1..1072
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             461..493
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     primer_bind     complement(571..589)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      585..632
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(807..812,813..829,830..835))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(862..880)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        948..1052
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taattttgtt taactttaag aaggagatat acatatggat gtattcatga aaggactttc
       61 aaaggccaag gagggagttg tggctgctgc tgagaaaacc aaacagggtg tggcagaagc
      121 agcaggaaag acaaaagagg gtgttctcta tgtaggctcc aaaaccaagg agggagtggt
      181 gcatggtgtg gcaacagtgg ctgagaagac caaagagcaa gtgacaaatg ttggaggagc
      241 agtggtgacg ggtgtgacag cagtagccca gaagacagtg gagggagcgg ggagcattgc
      301 agcagccact ggctttgtca aaaaggacca gttgggcaag aatgaagaag gagccccaga
      361 ggaaggaatt ctggaagata tgcctgtgga tcctgacaat gaggcttatg aaatgccttc
      421 tgaggaaggg tatcaagact atgaacctga agcctaacat atggctagca tgactggtgg
      481 acagcaaatg ggtcgcggat cccgggtacc tcgagtcgac cgatccggct gctaacaaag
      541 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
      601 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc
      661 ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac
      721 ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta
      781 gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg
      841 agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
      901 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
      961 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1021 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cg
//
