LOCUS       sequence-200212-        1067 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mutant human alpha synuclein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1067)
  TITLE     Lashuel Lab plasmids
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET11a asyn Q79N (Q109H) (TAC-->TAT)
FEATURES             Location/Qualifiers
     source          1..1067
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             472..504
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     primer_bind     complement(582..600)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      596..643
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(818..823,824..840,841..846))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(873..891)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aattcctnna naatattttg tttactttaa gaaggagata tacatatgga tgtattcatg
       61 aaaggacttt caaaggccaa ggagggagtt gtggctgctg ctgagaaaac caaacagggt
      121 gtggcagaag cagcaggaaa gacaaaagag ggtgttctct atgtaggctc caaaaccaag
      181 gagggagtgg tgcatggtgt ggcaacagtg gctgagaaga ccaaagagca agtgacaaat
      241 gttggaggag cagtggtgac gggtgtgaca gcagtagcca acaagacagt ggagggagcg
      301 gggagcattg cagcagccac tggctttgtc aaaaaggacc agttgggcaa gaatgaagaa
      361 ggagccccac acgaaggaat tctggaagat atgcctgtgg atcctgacaa tgaggcttat
      421 gaaatgcctt ctgaggaagg gtatcaagac tatgaacctg aagcctaaca tatggctagc
      481 atgactggtg gacagcaaat gggtcgcgga tcccgggtac ctcgagtcga ccgatccggc
      541 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
      601 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat
      661 atccggatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca
      721 tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc
      781 tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc
      841 tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
      901 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      961 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatganana
     1021 ataaccctga naaatgcttc aataatattg aaaaaggann ngtntga
//
