LOCUS       sequence-200213-        1115 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mutant human alpha synuclein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1115)
  TITLE     Lashuel Lab plasmids
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET11a asyn Q109N (TAC-->TAT)
FEATURES             Location/Qualifiers
     source          1..1115
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             457..489
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     primer_bind     complement(567..585)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      581..628
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(803..808,809..825,826..831))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(858..876)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        944..1048
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttgtttact ttaaagaagg agatatacat atggatgtat tcatnnaagg actttcaaag
       61 gccaaggagg gagttgtggc tgctgctgag aaaaccaaac agggtgtggc agaagcagca
      121 ggaaagacaa aagagggtgt tctctatgta ggctccaaaa ccaaggaggg agtggtgcat
      181 ggtgtggcaa cagtggctga gaagaccaaa gagcaagtga caaatgttgg aggagcagtg
      241 gtgacgggtg tgacagcagt agcccagaag acagtggagg gagcggggag cattgcagca
      301 gccactggct ttgtcaaaaa ggaccagttg ggcaagaatg aagaaggagc cccaaacgaa
      361 ggaattctgg aagatatgcc tgtggatcct gacaatgagg cttatgaaat gccttctgag
      421 gaagggtatc aagactatga acctgaagcc taacatatgg ctagcatgac tggtggacag
      481 caaatgggtc gcggatcccg ggtacctcga gtcgaccgat ccggctgcta acaaagcccg
      541 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
      601 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatcccgc
      661 aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg
      721 ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa
      781 tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
      841 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
      901 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      961 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     1021 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcncccttat
     1081 tccctttttt gnggcatttt gccttcctgn ttttg
//
