LOCUS       sequence-200214-         955 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mutant human alpha synuclein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 955)
  TITLE     Lashuel Lab plasmids
REFERENCE   2  (bases 1 to 955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET11a asyn Q79N Q109N (TAC-->TAT)
FEATURES             Location/Qualifiers
     source          1..955
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             462..494
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     primer_bind     complement(572..590)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      586..633
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(808..813,814..830,831..836))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(863..881)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata tacatatgga tgtattcatg aaaggacttt
       61 caaaggccaa ggagggagtt gtggctgctg ctgagaaaac caaacagggt gtggcagaag
      121 cagcaggaaa gacaaaagag ggtgttctct atgtaggctc caaaaccaag gagggagtgg
      181 tgcatggtgt ggcaacagtg gctgagaaga ccaaagagca agtgacaaat gttggaggag
      241 cagtggtgac gggtgtgaca gcagtagcca acaagacagt ggagggagcg gggagcattg
      301 cagcagccac tggctttgtc aaaaaggacc agttgggcaa gaatgaagaa ggagccccaa
      361 acgaaggaat tctggaagat atgcctgtgg atcctgacaa tgaggcttat gaaatgcctt
      421 ctgaggaagg gtatcaagac tatgaacctg aagcctaaca tatggctagc atgactggtg
      481 gacagcaaat gggtcgcgga tcccgggtac ctcgagtcga ccgatccggc tgctaacaaa
      541 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
      601 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat
      661 cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga
      721 cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt
      781 agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat
      841 gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
      901 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaa
//
