LOCUS       sequence-200216-        1144 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mutant human alpha synuclein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1144)
  TITLE     Lashuel Lab plasmids
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-7 asyn K80R
FEATURES             Location/Qualifiers
     source          1..1144
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             575..597
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(628..647)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(629..647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(712..734)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(969..986)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgattagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaat
       61 tctcatgttt gacagcttat catcgataag cttggatgga acatctgtca gcagatctca
      121 agaaactggg agcaaagata tttcttaggc ttcaggttcg tagtcttgat acccttcctc
      181 agaaggcatt tcataagcct cattgtcagg atccacaggc atatcttcca gaattccttc
      241 ctgtggggct ccttcttcat tcttgcccaa ctggtccttt ttgacaaagc cagtggctgc
      301 tgcaatgctc cctgctccct ccactgtcct ctgggctact gctgtcacac ccgtcaccac
      361 tgctcctcca acatttgtca cttgctcttt ggtcttctca gccactgttg ccacaccatg
      421 caccactccc tccttggttt tggagcctac atagagaaca ccctcttttg tctttcctgc
      481 tgcttctgcc acaccctgtt tggttttctc agcagcagcc acaactccct ccttggcctt
      541 tgaaagtcct ttcatgaata catccatatg tatatctcct tcttaaagtt aaacaaaatt
      601 atttctagag ggaaaccgtt gtggtctccc tatagtgagt cgtattaatt tcgaagtcta
      661 tcagaagttc gaatcgctgg gcctcgcgcg tttcggtgat gacggtgaan acctctgaca
      721 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
      781 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
      841 tagcgcatca gagcagattg tactgagagt gcaccatagg aagatcttcc ggaagatctt
      901 cctatgcggt gtgaaatacc gcacagatgc gtaaggagaa nataccgcat caggcgctct
      961 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     1021 gctcactcaa aggcggtaat acggttatcc ncagaatcag ggganaacgc aggaaanaac
     1081 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccnngtt gctggngttt
     1141 ttcc
//
