LOCUS       sequence-200219-         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mutant human alpha synuclein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  TITLE     Lashuel Lab plasmids
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-7 asyn WT1-106 Mxe
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             364..958
                     /codon_start=1
                     /gene="Mycobacterium xenopi gyrA"
                     /product="modified GyrA intein (Southworth et al., 1999)"
                     /label=Mxe GyrA intein
                     /note="N-terminal cleavage of this intein can be induced by
                     thiols."
                     /translation="CITGDALVALPEGESVRIADIVPGARPNSDNAIDLKVLDRHGNPV
                     LADRLFHSGEHPVYTVRTVEGLRVTGTANHPLLCLVDVAGVPTLLWKLIDEIKPGDYAV
                     IQRSAFSVDCAGFARGKPEFAPTTYTVGVPGLVRFLEAHHRDPDAQAIADELTDGRFYY
                     AKVASVTDAGVQPVYSLRVDTADHAFITNGXRQPR"
ORIGIN
        1 tttcctctnn nanattttgt ttaactttaa nnaggagata tacatatgga tgtattcatg
       61 aaaggacttt caaaggccaa ggagggagtt gtggctgctg ctgagaaaac caaacagggt
      121 gtggcagaag cagcaggaaa gacaaaagag ggtgttctct atgtaggctc caaaaccaag
      181 gagggagtgg tgcatggtgt ggcaacagtg gctgagaaga ccaaagagca agtgacaaat
      241 gttggaggag cagtggtgac gggtgtgaca gcagtagccc agaagacagt ggagggagca
      301 gggagcattg cagcagccac tggctttgtc aaaaaggacc agttgggcaa gaatgaagaa
      361 ggatgcatca cgggagatgc actagttgcc ctacccgagg gcgagtcggt acgcatcgcc
      421 gacatcgtgc cgggtgcgcg gcccaacagt gacaacgcca tcgacctgaa agtccttgac
      481 cggcatggca atcccgtgct cgccgaccgg ctgttccact ccggcgagca tccggtgtac
      541 acggtgcgta cggtcgaagg tctgcgtgtg acgggcaccg cgaaccaccc gttgttgtgt
      601 ttggtcgacg tcgccggggt gccgaccctg ctgtggaagc tgatcgacga aatcaagccg
      661 ggcgattacg cggtgattca acgcagcgca ttcagcgtcg actgtgcagg ttttgcccgc
      721 gggaaacccg aatttgcgcc cacaacctac acagtcggcg tccctggact ggtgcgtttc
      781 ttggaagcac accaccgaga cccggacgcc caagctatcg ccgacgagct gaccgacggg
      841 cggttctact acgcgaaagt cgccagtgtc accgacgccg gcgtgcagcc ggtgtatagc
      901 cttcgtgtcg acacggcaga ccacgcgttt atcacgaacg ggnttcgtca gccacgctac
      961 tggcctcacc ggtctgtagt gaggcctcac
//
