LOCUS       sequence-20022-e         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Huh KW, DeMasi J, Ogawa H, Nakatani Y, Howley PM, Munger K
  TITLE     Association of the human papillomavirus type 16 E7 oncoprotein with 
            the 600-kDa retinoblastoma protein-associated factor, p600.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Aug 9. 102(32):11492-7.
  PUBMED    16061792
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 Aug 9. 102(32):11492-7."
COMMENT     Sequence Label: pOZ-C 16 E7
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             347..370
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             386..412
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             386..412
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(629..646)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 acatcctcct agactgccag aatttccgct cgagaccatg catggagata cacctacatt
       61 gcatgaatat atgttagatt tgcaaccaga gacaactgat ctctactgtt atgagcaatt
      121 aaatgacagc tcagaggagg aggatgaaat agatggtcca gctggacaag cagaaccgga
      181 cagagcccat tacaatattg taaccttttg ttgcaagtgt gactctacgc ttcggttgtg
      241 cgtacaaagc acacacgtag acattcgtac tttggaagac ctgttaatgg gcacactagg
      301 aattgtgtgc cccatctgtt ctcagaaacc agcggccgct ggaggagact acaaggacga
      361 cgatgacaag tcggccgctg gaggataccc ctacgacgtg cccgactacg cctagggccg
      421 catcgagaga tccgggagac acaacggttt ccctctagcg ggatcaattc cgcccccccc
      481 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
      541 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
      601 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
      661 gtcgtgaagg aagcagttcc tctggaagc
//
