LOCUS       sequence-200220-         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mutant human alpha synuclein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Fares MB, Ait-Bouziad N, Dikiy I, Mbefo MK, Jovicic A, Kiely A, 
            Holton JL, Lee SJ, Gitler AD, Eliezer D, Lashuel HA
  TITLE     The novel Parkinson's disease linked mutation G51D attenuates in 
            vitro aggregation and membrane binding of alpha-synuclein, and 
            enhances its secretion and nuclear localization in cells.
  JOURNAL   Hum Mol Genet. 2014 Sep 1;23(17):4491-509. doi: 10.1093/hmg/ddu165. 
            Epub 2014 Apr 11.
  PUBMED    24728187
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/ddu165"; journalName: "Hum Mol Genet"; date: 
            "2014-09-1- 1"; volume: "23"; issue: "17"; pages: "4491-509"
COMMENT     Sequence Label: pT7-7 asyn G51D
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             572..594
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(625..644)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(626..644)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(709..731)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaattct
       61 catgtttgac agcttatcat cgataagctt ggatggaaca tctgtcagca gatctcaaga
      121 aactgggagc aaagatattt cttaggcttc aggttcgtag tcttgatacc cttcctcaga
      181 aggcatttca taagcctcat tgtcaggatc cacaggcata tcttccagaa ttccttcctg
      241 tggggctcct tcttcattct tgcccaactg gtcctttttg acaaagccag tggctgctgc
      301 aatgctccct gctccctcca ctgtcttctg ggctactgct gtcacacccg tcaccactgc
      361 tcctccaaca tttgtcactt gctctttggt cttctcagcc actgttgcca catcatgcac
      421 cactccctcc ttggttttgg agcctacata gagaacaccc tcttttgtct ttcctgctgc
      481 ttctgccaca ccctgtttgg ttttctcagc agcagccaca actccctcct tggcctttga
      541 aagtcctttc atgaatacat ccatatgtat atctccttct taaagttaaa caaaattatt
      601 tctagaggga aaccgttgtg gtctccctat agtgagtcgt attaatttcg aagtctatca
      661 gaagttcgaa tcgctgggcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
      721 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
      781 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc antcacgtag
      841 cgcatcagag cagattgtac tgagagtgca ccatangaag atcttccgga agatcttcct
      901 atgcggtgtg aaataccgca ca
//
