LOCUS       sequence-200223-       22030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector containing Sr22 Schomburgk allele driven by Sr33 
            promoter, Sr22 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 22030)
  AUTHORS   Md Hatta MA, Ghosh S, Athiyannan N, Richardson T, Steuernagel B, Yu 
            G, Rouse MN, Ayliffe M, Lagudah ES, Radhakrishnan GV, Periyannan SK,
            Wulff BBH
  TITLE     Extensive Genetic Variation at the Sr22 Wheat Stem Rust Resistance 
            Gene Locus in the Grasses Revealed Through Evolutionary Genomics and
            Functional Analyses.
  JOURNAL   Mol Plant Microbe Interact. 2020 Nov;33(11):1286-1298. doi: 
            10.1094/MPMI-01-20-0018-R. Epub 2020 Oct 1.
  PUBMED    32779520
REFERENCE   2  (bases 1 to 22030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-01-20-0018-R"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-11"; volume: "33"; issue: "11"; pages: 
            "1286-1298"
COMMENT     Sequence Label: pBW_0003
FEATURES             Location/Qualifiers
     source          1..22030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    416..440
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        1482..1827
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1770..1790
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    1857..1912
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             1914..2465
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      2488..3195
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(13188..13371)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(13224..13403)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    13527..13551
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      14039..14674
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(15349..16137)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      17234..17822
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     17723..17742
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     17976..17993
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(19182..20330)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     mobile_element  20955..21722
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     oriT            21782..21891
                     /note="incP origin of transfer"
ORIGIN
        1 gtcgacgatc gtcaacgttc acttctaaag aaatagcgcc actcagcttc ctcagcggct
       61 ttatccagcg atttcctatt atgtcggcat agttctcaag atcgacagcc tgtcacggtt
      121 aagcgagaaa tgaataagaa ggctgataat tcggatctct gcgagggaga tgatatttga
      181 tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc
      241 cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca
      301 aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg
      361 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca
      421 ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt
      481 ttttaatgta ctgaattcca atcccacaaa aatctgagct taacagcaca gttgctcctc
      541 tcagagcaga atcgggtatt caacaccctc atatcaacta ctacgttgtg tataacggtc
      601 cacatgccgg tatatacgat gactggggtt gtacaaaggc ggcaacaaac ggcgttcccg
      661 gagttgcaca caagaaattt gccactatta cagaggcaag agcagcagct gacgcgtaca
      721 caacaagtca gcaaacagac aggttgaact tcatccccaa aggagaagct caactcaagc
      781 ccaagagctt tgctaaggcc ctaacaagcc caccaaagca aaaagcccac tggctcacgc
      841 taggaaccaa aaggcccagc agtgatccag ccccaaaaga gatctccttt gccccggaga
      901 ttacaatgga cgatttcctc tatctttacg atctaggaag gaagttcgaa ggtgaaggtg
      961 acgacactat gttcaccact gataatgaga aggttagcct cttcaatttc agaaagaatg
     1021 ctgacccaca gatggttaga gaggcctacg cagcaggtct catcaagacg atctacccga
     1081 gtaacaatct ccaggagatc aaataccttc ccaagaaggt taaagatgca gtcaaaagat
     1141 tcaggactaa ttgcatcaag aacacagaga aagacatatt tctcaagatc agaagtacta
     1201 ttccagtatg gacgattcaa ggcttgcttc ataaaccaag gcaagtaata gagattggag
     1261 tctctaaaaa ggtagttcct actgaatcta aggccatgca tggagtctaa gattcaaatc
     1321 gaggatctaa cagaactcgc cgtgaagact ggcgaacagt tcatacagag tcttttacga
     1381 ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacactct ggtctactcc
     1441 aaaaatgtca aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg
     1501 ataatttcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg
     1561 acagtagaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggctatc
     1621 attcaagatc tctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
     1681 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgacatctcc
     1741 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
     1801 ggaagttcat ttcatttgga gaggacacgc tcgagtataa gagctctatt tttacaacaa
     1861 ttaccaacaa caacaaacaa caaacaacat tacaattact atttacaatt accatgagcc
     1921 cagaacgacg cccggccgac atccgccgtg ccaccgaggc ggacatgccg gcggtctgca
     1981 ccatcgtcaa ccactacatc gagacaagca cggtcaactt ccgtaccgag ccgcaggaac
     2041 cgcaggagtg gacggacgac ctcgtccgtc tgcgggagcg ctatccctgg ctcgtcgccg
     2101 aggtggacgg cgaggtcgcc ggcatcgcct acgcgggccc ctggaaggca cgcaacgcct
     2161 acgactggac ggccgagtca accgtgtacg tctccccccg ccaccagcgg acgggactgg
     2221 gctccacgct ctacacccac ctgctgaagt ccctggaggc acagggcttc aagagcgtgg
     2281 tcgctgtcat cgggctgccc aacgacccga gcgtgcgcat gcacgaggcg ctcggatatg
     2341 ccccccgcgg catgctgcgg gcggccggct tcaagcacgg gaactggcat gacgtgggtt
     2401 tctggcagct ggacttcagc ctgccggtac cgccccgtcc ggtcctgccc gtcaccgaga
     2461 tttgatgacc cgggggatcc tagagtcctg ctttaatgag atatgcgaga cgcctatgat
     2521 cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc
     2581 tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt tactatcgta
     2641 tttttatgaa taatattctc cgttcaattt actgattgta ccctactact tatatgtaca
     2701 atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc tatgatagag
     2761 cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa aaaagcggca
     2821 gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca aaagtgcccc
     2881 aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg aagggaactc
     2941 cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg ccgtccgctc
     3001 taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt aaccgacttg
     3061 ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg aagtgcaggt
     3121 caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt gcgacaacat
     3181 gtcgaggctc agcaggacct gcaggcatgc aagctctaga gcggccgccg ccgcgacgac
     3241 tcgacgatta gtcggcgatt agtcggcgac tcaaaaacct tgtaaacagt caacatcgtg
     3301 gtaatatacg caccacaaac ttgaaaactt gagcaaaaac accatgataa ctttgacctg
     3361 gatggaggaa gttgttgaaa agcataccct cggtaattta tgtgcaaata acatgataat
     3421 ttacccatcg gagagctaat aactctcgtt caaacaccac aataattttt tgaccctgaa
     3481 ttttttttcg ttgaagaaca ttacccccaa taacttctag gtaaatagca cggtaatata
     3541 tttattgcag atacaataac ttacgcaaaa acagtatgct agctttgacc cacgtaaaaa
     3601 attgtttgaa gcatatcgtt ggtaaatttt gtttaaatag aatggtaata tacgcattgc
     3661 agacatgata agttactcac aaacatcatg ataagtttga ttcgggtgga tgaagttgtt
     3721 gaaaatatac ccggtaactt gtgtgtaaac agcacggtaa tagtcttgat ttggggaaga
     3781 atagtaaaaa attgtcaaaa aatacactgg taacttttgt atgtaaatag catagtaaat
     3841 taggcaccac aaacttgata atttagatac aaacaccgcg gtaaattaga gattttttta
     3901 aaagatagcc atggtaatat acccatcgga tacctgataa cttacgtaca tatactatgg
     3961 taatatttga ctcggtgggg aaattattga aaacagacac cccccccccc cctcccggta
     4021 acttatatgt aaacatcatg gtaatatatg aactgtagtg tcggtgaacc atttttatgc
     4081 ctccgatgac ttatgtggaa atagcacggt aacttgcata taaaaggcat ggtaaatttt
     4141 ggttaaaaaa agtcatcgaa acatgtcaac ataggatcta gtttcaaaga tctcatcgcg
     4201 acgaatcgta tatgtaaaaa cggtttttac atcggaccga cggtttgagc tacaaaacat
     4261 tttaaatatt ttaataaaga aaatctagaa tgacatcgtc ttttttgtcc tctaatgtat
     4321 gcatctaatt agaaggagaa atgcacgtga aagaagaaag aatcaaacta atgtatacat
     4381 ctatttagag tgaaggaaat agaggtgtga cagttttgtt tatatggcac acatgtgcca
     4441 aatatcatag tccgatagaa tatagatgca cgctagatct ttttcttctg ataagctaca
     4501 cggtatgtgt ttcctaacaa cagaaatctg ggttacatat caaagtctca atctaagaat
     4561 acacaaatat taatggaaaa aaatactcaa atgacaatct acttatgccc aaaagaaaag
     4621 aaaaaaattg catccgactc tgaattcgtg atacttgtct tacttattcc tagtcattcc
     4681 actaattcgt gatacttgca gacatgtaca gaagtatcat ttgctgcagc atccaatccg
     4741 agtacgtgac ttgggctcca taaccaaagg cgcatctcgg tttaggcaaa gcctaggact
     4801 tgacggccgg cgttgtagct gaaatagatt accggaaaaa agattgaagc atatgcacat
     4861 cttttgatct actgctcacc aagacaaaag tacaaactag ttaatagtac ctgagcctgt
     4921 catacctatt tgctcgtctt gtaacatatg ctgcgtatta tatcttgtca tctccagtat
     4981 tggacggttg gggttcgcct cggccatgct cttgaaagca tcctccgcag cctctacttc
     5041 tgccaccgtc cagccaccgc aggataagct gacagtgagg cgagcaaggc tggagaggcg
     5101 ttggatgcca aagtcgaaat cgaccgcagc agatttgggc ttttcgatga agttatggag
     5161 tttaagctcc ttgagcctcg gcatagcccc cggttcaaac attaaaaccc ccatacgagt
     5221 attgtaagcc tcaaacttct gcaactgttg aaatccttca cgcctgatga tgagccgccc
     5281 agcgtcgtct gttttgtata gaggcggcag tgtcaaatcg atcagattgg gtatcaatcc
     5341 aatcatctca atatcctgct gctctatctt cggatcgtcc aaaagcaacc tgtgtaggtt
     5401 gggaagtgac gcgagccatc tgctaatccg gctgatatct tgtccatata caaaaacctc
     5461 ctggatacaa cccaaagcag ggaggaacgg ctcttctcca tccttttctc tcagataata
     5521 atcaatactc agggaaagga ggccgcattc gtccagttta cagagagagg acaccagctt
     5581 ttccttggac tcttctaatt tgttgccttc aatcccacca gtgtccaatt taatactcag
     5641 ctttctcaga tttgttagtt tgccgagctc ttccacatgc ttcaaatgca aggcatcaac
     5701 gcagtcaagc tcttgcaagt ttatcaagtt tcccaaccca cctggaagct tacaaccaga
     5761 gtaaacaaac aaacgtgcca gtcgttgtag ccgacttgtg ctttcaggaa gctcacgtaa
     5821 tccataggca actagcgtct ctagatacag caactcccca atttgcctgg gaagctccgt
     5881 gattcctgta gaagagatat ccaagtatct tagctgatag agtctttcaa tatccttgat
     5941 atgatgattt tccaactgat cgcagtcttg caagtctagc acgcgcaagc attttgacct
     6001 cccaagcgaa ggcaatagct tgatatcccc aaaagcagtg attgaccgag cgtgtgacag
     6061 atccaattgc tccacgattt cttcatcctc attccccatt agagagagcc gacgaactat
     6121 gccattaggc ttgcattgtt tctttaggac agtacacatg ttctcttcag aggatttgtc
     6181 aattaggaaa tcaaggatgg tgtcatggac tcggcaatac attgccttgc cgtcataccc
     6241 tatgttgccg ggctgtatta gacttctgtt cacaagctgg tgaaaatatt cctctcctaa
     6301 ttccatgaga tcttgtccac tttctccatg gataaagcct tcagaaatcc atctgcgtac
     6361 taaccgcttc cttccaatca attggtcttc aggaaacaga gccaggtaca ataagcagct
     6421 tcttaggtgg tggggaagat caaaataact aagagacaat atgtaattca tggtttctat
     6481 tatatcactt ttgacctttg aagagcgtat tgaatgtcgc acccgatccc actcttcttt
     6541 tgtttccctt gttgtgacca acacgctaga aatggcattg atggccagag gtaggccacg
     6601 gcattttttc aaaatatcat tggcagcttc tttcaagcta tcaggacagc tttcttcaca
     6661 gccaaatatc ctcttgaaaa acaatttctt cgagtcagcc tcactgagtg gtttcatctc
     6721 atagacaaga tcttcactag aaggagaaca agatttggca acatcaacta tacgtgttgt
     6781 gataattatt ctgctgccca atgtattctt gacaaatgca catttcaaag tttcccatgt
     6841 tcctaactcc catatatcat caattatgat aaagtacctg caacacagtt tcatgaacat
     6901 acacattaga gctcattaga tatttatagt aacacctacc aagtaaccta aaatcctgaa
     6961 acaatctcac atctgtgtgc atcctagata taatacacct tgatgtaatt gtctagcatc
     7021 aagaaagttg tcattatcta aaataagtga aatcctgaga gcatctcaca ataatacaca
     7081 gctacagatg ggggagaaac tcaaagataa caactccaca catagtagta ctaggtacaa
     7141 gcaactctaa cacgccaacg gatttggtcc gtgtgtgtcc attagggtcg aaaggcacaa
     7201 aaacgccggc ccaacgtgcg gacacaaacc caaatcacgt ccgttcggca tccatgtaga
     7261 cgcatttcca gcccaaattt gtgcctgatt tgcgttcaca cgaacacgag acggacgtgt
     7321 tgcgcgtccg ctcggtgtgc gcctcgggct ggcgtgtctg ccagccaact accaccacag
     7381 acgtagtcaa tggcgcccac ctacctcggg cccgcatggc agtgactaga aacgtccgga
     7441 tcacgccctt tttaattgca agggtgcggc ggggcgggcc tcatccactt ctcctcccac
     7501 tctagtccac atctgctccc tcgccggtga cagaaaaccc tagccgtcat gggtttcttt
     7561 ggcaaaggta agggcaaggc aagcacgacc acgaggccgg ctcgttgcaa gggccggtga
     7621 ggccggcagc gaaggggttc gcattggtgt cggcgctgcg ggatgggagg gcggcagcgc
     7681 cacgggacca ccagtacatc ccggtgcacc acgcgtagtt agtaccaccg ggagtaccgc
     7741 gtctcactgc cgtggccgga cgtgaacttc ccccacggct ggcacctcga cccccaccac
     7801 attccagtgc cggcggtgcc acagtccgag cgtgcgcgcg ccaacgagat tcgggcccgg
     7861 cgtgctctcc tgacgccaga gcagcgtcgc gactccaccc atcgaggcca gactccccta
     7921 actgagaagt ctggttcacc atcgagcacc atgtgcgcct ccgcgacgcc gtccagctcg
     7981 aagcctgccc tccaccgccg ccaccggtgg tcaaatagta ggttgaggag gaggcggcgt
     8041 tcctcaaggc attggaggag ggggcccatc acgtcaccga ggagtcttag tgcgaggact
     8101 cctagtggtc tggcctcgat gacacgctgc agctatcggc cgccagctat agtgtctacc
     8161 tgccgggacc accagccaca tcgtcaccgc ctgcaccagc gctagcacca cccgcgccca
     8221 ccggcgttta ccaatggcag ggcatcgtct gcaagcggat gagcaagccg cccatttggc
     8281 tgtgcgtgat ggaggaggag gcggcctacc tcgtggcgtg gaaggaccgc acgctggagg
     8341 aggagcggta cgacggccag gtccgcatgc agcgggaacc cgagggggag gagcgccaaa
     8401 acttcgttgg gcgtgcgatg aggaggagga gcggcaggag cgcgaggagg aggcgtgcgc
     8461 ggtgccaggt agggccgccg ccgccgcaga cagcggagca ggcggtgctg gctgcccacc
     8521 agcaggcctt cccgtgggct gagctgccgg cattcttcga cctcacccac tcctccgacg
     8581 aagacgccta gggctgcggg ctggggcggc tgttttttat taatatatta atgtattatg
     8641 tttaattatg tggactttga cgggctttaa tatgcatttt tatttatatt taattatttt
     8701 aagcttttta agttttttaa atgcccacgc ggactaatgc gtaggcgcac cacccaaagg
     8761 caaaaccaga caccccgtcc gcttggccga tctaaatgga caaaaattgc gtcggcgtgt
     8821 tggagttgct cttagataca atttggtggc aatataagaa aaaaaaagat tgccatagaa
     8881 ggcaagcgaa aaatagcaag atttaacaac caagaggaaa aaaatatgaa tgctcaggcc
     8941 gagtaaaggc agctgttgcc agtggcaagg tacaatgttt tgccactgcc aatagcatgt
     9001 tcgcaagagg atagatattg aaacaatgat ggggactacc tattttcttt tgtcattttg
     9061 gtaacaagtt atttcctcat gattggtagg accacatatt gagtggtggc tctgcgcctc
     9121 aattcgtcat gcaaattgct acgaaaccag gaaagacgca acaacctact gttgtcatta
     9181 tgttacgcta gtcaggctgg tccgatctga ctatgattaa ttcttccctt ttctcttttt
     9241 gtgttatggg catagattct tcaacggatg tttgatatcc attcaagatt ataatactga
     9301 taaccgtagg tttatagcct ctagaaaccc tatgttctta aagtggctaa tcacaaacct
     9361 aatgtactgc atatccggta acaatggtct gcacatgcaa gtgctttggg tccttagaag
     9421 atagatatat ttgcagtcat gtagtgcgtg cggatattta tcttgtacaa aagagaggga
     9481 attgcatgta ctttattgtt ttgactgacg ccatgtcaca attatttaat aaacagaaca
     9541 tagccgcaag tttaacaaac ttgtggcaat caaattagaa aatgtgtggc gacagaaatc
     9601 ggcaaacaca ttttagctat atcttaatta gtgaacagat cgacataagt tgcaagaaac
     9661 aatgacttga aagtttaagc gcagtcatac aagttgagga cgaaagtaaa tcacacaaag
     9721 caatcttcat tagcaaaatt ctaggcaacc gattatgcat gagctaatta agcaatgtta
     9781 cttcgtagca gatctcacat acataccttg tgtcttggag gaattctcga atcttgtcga
     9841 tgatgagttg tatatcatct aatctagagc aagcatcgtc tttcttggtg atttgtgata
     9901 atatagatct taatatcgcc ttcatgtcag gcttgcgtga aatcgagaca aaagccccgc
     9961 agtcgaaggt cgctccaagg ttgacccgga tctggttggc gagcgtggtc ttgcccaacc
    10021 ctccatatcc aacaatagag acagatttca gtgattggcc ttctttctca ttcagccact
    10081 tgacaagttc atcctttggt ccatcaattc cgacgagctc tgcggcgtct ttgtagactg
    10141 cacgaagtcg agggtcgacc ttgtcttttg ccggcataga ggaggaggag tcgtcaatct
    10201 tgtaccttga gtggcaacaa atcggtcaca aatctgatgg taggtactaa tagtactgta
    10261 aggagaacaa gatctggtac cttgaaaatc tgtcgctgac atccttaatt tctttcttga
    10321 tgtctttgac ctccttggcg atcctgcggc gggccttgat gtccggcagc aagttcttgc
    10381 acttgtccat gagcttggcg acgccatgag ccgcctcgga acaggaggac ccgtgttcct
    10441 gttccacgag gaccatgaac ttgtcgatgt tgtcctctat cttataggac atctcccgca
    10501 ccgccgtcac acgcagcttt gttggttcgt caggctcctc cacatctgac atcttgagga
    10561 ggaacgcgca catgacctcc agctcgtcct tgaggaactt gatgtcccca cggacgttct
    10621 tgagcagctt gtattcatcc gtcaacatag ctcccaactt ccgcaggagg gaacccattg
    10681 cccctgtgga tgcgctcacc agaacttccg ccatcattga gagctctctt gatcaatcag
    10741 ctgaaacctg caaatcgatg aatttaggga tctgtaacac aatccaagct ctggtggaac
    10801 tgagttggca agccaaatcg atgaatgtgg ggatctggac caagcccacc ttttgatgct
    10861 tcagaggaga gcagaacaca acggcggcgt cttcgtcgga tggtcacgct gtgcggacaa
    10921 cgaagcgggt cagtggatta aacgctttgc tcgatgggga caacacggcg gccctgtaat
    10981 aaaaacttat agccgggtca gcgccttgga tgcatggtgc tctgaataat gactgggcaa
    11041 atcaatgaga ctcgtcaagc aggagaagca atgacttttt agacccaagc attgctactt
    11101 tactggaaac aaatcgacaa gaaacatacc cggaggttgg atgagattag caacaaggtg
    11161 gatggatctg ttcaaggcag cacaatggaa gcggggacag aggaagtcca acagcagtca
    11221 agagagtaaa ttgcttggaa tacagtggta ctccgtacta gatagataca gagccccacg
    11281 accccaccct ggattcaatc aacacaaatc ggtcagaatc tcaagcagga gaagtaataa
    11341 cttttagatc cctttttaga tccaataact ggtaaaaaaa cagagcagag gaagacccca
    11401 ctgccacggc cacgggtgtc aaggtcggat cgccgtcagt ggagaaagag cagaggaaga
    11461 ccccaccttt ttgtgcttca cggcagagca gaacacagcg gcggtggcct cgtcggacaa
    11521 cgaagcgaaa gcggcggctg ggcaccagag gccagatccg tgaggggaca agacggcgct
    11581 tcactcaatc tggaagtata atctgggaaa tcagcagatg aacaaggaag gtggggatgt
    11641 ggaagatttc agtagcaaga aagatgagac aaagttacca ggtcagtgga ttccatgcgt
    11701 ggtgctctga ataatgattg gaaactccat gattcttgct agctgctcca ctgccacccc
    11761 aaatgattca accaacacaa atcgatcaga ctcccaagca agcagtgaag caggagacgt
    11821 aagtttttag atccaagcgt acttcagctg gaaagaaata tacctccact ccacaggtgt
    11881 caggggcgag ctcggaggtt ggacgagctc accaaacaag aagccggatc gaccaggaga
    11941 tgaggactga gatgatagga agggagcact ctgtcggatc cgtgagcgtg tgggcaccag
    12001 aggccagatc cgtgagcgtg tggaggtcag tggattcgat gcatggtgca aaatagatga
    12061 atatggggag ctgtaatcca atcaaaactg tgtgaagctt taatttcaac gagctataga
    12121 acagagttgc caatccaaac tgtgatatcc atccaaaccc acctttgata cttcacggca
    12181 gaggagaaca cagccgcggc gtcaacaccg gatggccgtc accgttgatg cttcaccggc
    12241 agaggaggac agagcagaac acagcggcgt cgtaggggac aagacggcgg cggatgtcgc
    12301 ttcactcgat ctggaagaat aagtaagaac ttaccatcaa gcaaatcagc agattaataa
    12361 gaagagaata gcaagaaaga tgggagggaa atttttacca ggccactagt atggtgctct
    12421 gaatagtcac tggaaactgc cacccgaaat ctccacggag gatggatgag atcagcaaca
    12481 aggcggatcg atctgttcca ggcagcgcaa tggaggagac tgagaggaag cggagacgag
    12541 gagcagagga agaccaacag cactcaagag agtaaattgc ttggaatatc catcctagat
    12601 agatacagag ccccacgatc ccaccctgag ttcaatcaac acaaatggat cagaatctga
    12661 agcaggagaa gtaataagtt ttagatccaa gcattgctac tttaagtgga aacaaatcga
    12721 caggaaatat atatacccaa aaagtccacg gtcaccaata gagcagtcag ttggcagggg
    12781 cgaggccaat aggaggcgag atgagatcag caacaagggc ggatctatct tattcgaggg
    12841 aggaggagag gactgagatg agaatgagga gaggaaggga gtactcagtg gagggagacg
    12901 aggagcatca agagaggata ctactttagc acgctcctcg aatactagta cgtattacag
    12961 ggagggatac cctctgcctt tgggctgata tgtttattca gtgggtacct atggtcagtc
    13021 caccagccat gcttcaccaa atattatccc taaatagccc ttttgaaatt taagtaagca
    13081 catcctcaac actcttaaag cggccgccac cgcggctgag tggctccttc aagcttgcgg
    13141 atccgggcgg aaataggtaa agaagttgcg gataaggtaa ttgccattgc agattatttg
    13201 gattgagagt gaatatgaga ctctaattgg ataccgaggg gaatttatgg aacgtcagtg
    13261 gagcattttt gacaagaaat atttgctagc tgatagtgac cttaggcgac ttttgaacgc
    13321 gcaataatgg tttctgacgt atgtgcttag ctcattaaac tccagaaacc cgcggctgag
    13381 tggctccttc aacgttgcgg ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca
    13441 tcggcggggg tcataacgtg actcccttaa ttctccgctc atgatcagat tgtcgtttcc
    13501 cgccttcagt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa
    13561 aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt
    13621 ccatttgtat gtgcatgcca accacagggt tcccctcggg agtgcttggc attccgtgcg
    13681 ataatgactt ctgttcaacc acccaaacgt cggaaagcct gacgacggag cagcattcca
    13741 aaaagatccc ttggctcgtc tgggtcggct agaaggtcga gtgggctgct gtggcttgat
    13801 ccctcaacgc ggtcgcggac gtagcgcagc gccgaaaaat cctcgatcgc aaatccgacg
    13861 ctgtcgaaaa gcgtgatctg cttgtcgctc tttcggccga cgtcctggcc agtcatcacg
    13921 cgccaaagtt ccgtcacagg atgatctggc gcgagttgct ggatctcgcc ttcaatccgg
    13981 gtctgtggcg ggaactccac gaaaatatcc gaacgcagca agatcgtcga ccctttccga
    14041 cgctcaccgg gctggttgcc ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg
    14101 cgccagaaac gccgtcgaag ccgtgtgcga gacaccgcgg ccggccgccg gcgttgtgga
    14161 tacctcgcgg aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg
    14221 gccgactcac ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg
    14281 tggagctggc cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag
    14341 atgatgtgga caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact
    14401 actgacagat gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg
    14461 gcgcacctat tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt
    14521 ttccgcccgt ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt
    14581 ataaaccttg tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg
    14641 ggtgcccccc cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg
    14701 gctgcgcccc tcggccgcga acggcctcac cccaaaaatg gcagccaagc tcctaacatt
    14761 ttattagaga gcaggctagt tgcttagata catgatcttc aggccgttat ctgtcagggc
    14821 aagcgaaaat tggccattta tgacgaccaa tgccccgcag aagctcccat ctttgccgcc
    14881 atagacgccg cgcccccctt ttggggtgta gaacatcctt ttgccagatg tggaaaagaa
    14941 gttcgttgtc ccattgttgg caatgacgta gtagccggcg aaagtgcgag acccatttgc
    15001 gctatatata agcctacgat ttccgttgcg actattgtcg taattggatg aactattatc
    15061 gtagttgctc tcagagttgt cgtaatttga tggactattg tcgtaattgc ttatggagtt
    15121 gtcgtagttg cttggagaaa tgtcgtagtt ggatggggag tagtcatagg gaagacgagc
    15181 ttcatccact aaaacaattg gcaggtcagc aagtgcctgc cccgatgcca tcgcaagtac
    15241 gaggcttaga accaccttca acagatcgcg catagtcttc cccagctctc taacgcttga
    15301 gttaagccgc gccgcgaagc ggcgtcggct tgaacgaatt gttagacatt atttgccgac
    15361 taccttggtg atctcgcctt tcacgtagtg aacaaattct tccaactgat ctgcgcgcga
    15421 ggccaagcga tcttcttgtc caagataagc ctgcctagct tcaagtatga cgggctgata
    15481 ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc
    15541 ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc
    15601 ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg
    15661 ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc
    15721 tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc
    15781 tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg
    15841 ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct
    15901 ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc
    15961 atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc
    16021 atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat
    16081 gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt ccctcatgat
    16141 gtttaactcc tgaattaagc cgcgccgcga agcggtgtcg gcttgaatga attgttaggc
    16201 gtcatcctgt gctcccgaga accagtacca gtacatcgct gtttcgttcg agacttgagg
    16261 tctagtttta tacgtgaaca ggtcaatgcc gccgagagta aagccacatt ttgcgtacaa
    16321 attgcaggca ggtacattgt tcgtttgtgt ctctaatcgt atgccaagga gctgtctgct
    16381 tagtgcccac tttttcgcaa attcgatgag actgtgcgcg actcctttgc ctcggtgcgt
    16441 gtgcgacaca acaatgtgtt cgatagaggc tagatcgttc catgttgagt tgagttcaat
    16501 cttcccgaca agctcttggt cgatgaatgc gccatagcaa gcagagtctt catcagagtc
    16561 atcatccgag atgtaatcct tccggtaggg gctcacactt ctggtagata gttcaaagcc
    16621 ttggtcggat aggtgcacat cgaacacttc acgaacaatg aaatggttct cagcatccaa
    16681 tgtttccgcc acctgctcag ggatcaccga aatcttcata tgacgcctaa cgcctggcac
    16741 agcggatcgc aaacctggcg cggcttttgg cacaaaaggc gtgacaggtt tgcgaatccg
    16801 ttgctgccac ttgtttaata gactggatgg aggcggataa agttgcagga ccacttctgc
    16861 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    16921 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    16981 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    17041 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    17101 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    17161 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    17221 tcaaaggatc ttcttgatat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    17281 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    17341 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
    17401 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    17461 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    17521 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    17581 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    17641 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    17701 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    17761 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    17821 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    17881 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    17941 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    18001 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
    18061 gaagatcggc ggcaatagct tcttagcgcc atcccggctg agaaagccca gtaaggaaac
    18121 aactgtaggt tcgagtcgcg agatcccccg gaaccaaagg aagtaggtta aacccgctcc
    18181 gatcaggccg agccacgcca ggccgagaac attggttcct gtaggcatcg ggattggcgg
    18241 atcaaacact aaagctactg gaacgagcag aagtcctccg gccgccagtt gccaggcggt
    18301 aaaggtgagc agaggcacgg gaggttgcca cttgcgggtc agcacggttc cgaacgccat
    18361 ggaaaccgcc cccgccaggc ccgctgcgac gccgacagga tctagcgctg cgtttggtgt
    18421 caacaccaac agcgccacgc ccgcagttcc gcaaatagcc cccaggaccg ccatcaatcg
    18481 tatcgggcta cctagcagag cggcagagat gaacacgacc atcagcggct gcacagcgcc
    18541 taccgtcgcc gcgaccccgc ccggcaggcg gtagaccgaa ataaacaaca agctccagaa
    18601 tagcgaaata ttaagtgcgc cgaggatgaa gatgcgcatc caccagattc ccgttggaat
    18661 ctgtcggacg atcatcacga gcaataaacc cgccggcaac gcccgcagca gcataccggc
    18721 gacccctcgg cctcgctgtt cgggctccac gaaaacgccg gacagatgcg ccttgtgagc
    18781 gtccttgggg ccgtcctcct gtttgaagac cgacagccca atgatctcgc cgtcgatgta
    18841 ggcgccgaat gccacggcat ctcgcaaccg ttcagcgaac gcctccatgg gctttttctc
    18901 ctcgtgctcg taaacggacc cgaacatctc tggagctttc ttcagggccg acaatcggat
    18961 ctcgcggaaa tcctgcacgt cggccgctcc aagccgtcga atctgagcct taatcacaat
    19021 tgtcaatttt aatcctctgt ttatcggcag ttcgtagagc gcgccgtgcg tcccgagcga
    19081 tactgagcga agcaagtgcg tcgagcagtg cccgcttgtt cctgaaatgc cagtaaagcg
    19141 ctggctgctg aacccccagc cggaactgac cccacaaggc cctagcgttt gcaatgcacc
    19201 aggtcatcat tgacccaggc gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct
    19261 tcgccgacct gctcgcgcca cttcttcacg cgggtggaat ccgatccgca catgaggcgg
    19321 aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt
    19381 cgggccgtcg gcgacagctt gcggtacttc tcccatatga atttcgtgta gtggtcgcca
    19441 gcaaacagca cgacgatttc ctcgtcgatc aggacctggc aacgggacgt tttcttgcca
    19501 cggtccagga cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg
    19561 gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa
    19621 taccggccat tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc
    19681 ggctcgccga taggggtgcg cttcgcgtac tccaacacct gctgccacac cagttcgtca
    19741 tcgtcggccc gcagctcgac gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa
    19801 atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca
    19861 gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc aatatcgaac
    19921 aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg
    19981 gcctcgctga cctgttttgc caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata
    20041 gttcctcgcg tgtcgatggt catcgacttc gccaaacctg ccgcctcctg ttcgagacga
    20101 cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag ggggagccag ttgcacgctg
    20161 tcgcgctcga tcttggccgt agcttgctgg accatcgagc cgacggactg gaaggtttcg
    20221 cggggcgcac gcatgacggt gcggcttgcg atggtttcgg catcctcggc ggaaaacccc
    20281 gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg tccgattcat tcaccctcct
    20341 tgcgggattg ccccgactca cgccggggca atgtgccctt attcctgatt tgacccgcct
    20401 ggtgccttgg tgtccagata atccacctta tcggcaatga agtcggtccc gtagaccgtc
    20461 tggccgtcct tctcgtactt ggtattccga atcttgccct gcacgaatac cagctccgcg
    20521 aagtcgctct tcttgatgga gcgcatgggg acgtgcttgg caatcacgcg caccccccgg
    20581 ccgttttagc ggctaaaaaa gtcatggctc tgccctcggg cggaccacgc ccatcatgac
    20641 cttgccaagc tcgtcctgct tctcttcgat cttcgccagc agggcgagga tcgtggcatc
    20701 accgaaccgc gccgtgcgcg ggtcgtcggt gagccagagt ttcagcaggc cgcccaggcg
    20761 gcccaggtcg ccattgatgc gggccagctc gcggacgtgc tcatagtcca cgacgcccgt
    20821 gattttgtag ccctggccga cggccagcag gtaggccgac aggctcatgc cggccgccgc
    20881 cgccttttcc tcaatcgctc ttcgttcgtc tggaaggcag tacaccttga taggtgggct
    20941 gcccttcctg gttgggtaat gactccaact tattgatagt gttttatgtt cagataatgc
    21001 ccgatgactt tgtcatgcag ctccaccgat tttgagaacg acagcgactt ccgtcccagc
    21061 cgtgccaggt gctgcctcag attcaggtta tgccgctcaa ttcgctgcgt atatcgcttg
    21121 ctgattacgt gcagctttcc cttcaggcgg gattcataca gcggccagcc atccgtcatc
    21181 catatcacca cgtcaaaggg tgacagcagg ctcataagac gccccagcgt cgccatagtg
    21241 cgttcaccga atacgtgcgc aacaaccgtc ttccggagac tgtcatacgc gtaaaacagc
    21301 cagcgctggc gcgatttagc cccgacatag ccccactgtt cgtccatttc cgcgcagacg
    21361 atgacgtcac tgcccggctg tatgcgcgag gttaccgact gcggcctgag ttttttaagt
    21421 gacgtaaaat cgtgttgagg ccaacgccca taatgcgggc tgttgcccgg catccaacgc
    21481 cattcatggc catatcaatg attttctggt gcgtaccggg ttgagaagcg gtgtaagtga
    21541 actgcagttg ccatgtttta cggcagtgag agcagagata gcgctgatgt ccggcggtgc
    21601 ttttgccgtt acgcaccacc ccgtcagtag ctgaacagga gggacagctg atagaaacag
    21661 aagccactgg agcacctcaa aaacaccatc atacactaaa tcagtaagtt ggcagcatca
    21721 cccctggttg gcttggtttc atcagccatc cgcttgccct catctgttac gccggcggta
    21781 gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca
    21841 gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc
    21901 cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg
    21961 aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg
    22021 gaaaagatcc
//
