LOCUS       sequence-200226-       21881 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector containing Sr22 PI190945 allele driven by Sr33 
            promoter, Sr33 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21881)
  AUTHORS   Md Hatta MA, Ghosh S, Athiyannan N, Richardson T, Steuernagel B, Yu 
            G, Rouse MN, Ayliffe M, Lagudah ES, Radhakrishnan GV, Periyannan SK,
            Wulff BBH
  TITLE     Extensive Genetic Variation at the Sr22 Wheat Stem Rust Resistance 
            Gene Locus in the Grasses Revealed Through Evolutionary Genomics and
            Functional Analyses.
  JOURNAL   Mol Plant Microbe Interact. 2020 Nov;33(11):1286-1298. doi: 
            10.1094/MPMI-01-20-0018-R. Epub 2020 Oct 1.
  PUBMED    32779520
REFERENCE   2  (bases 1 to 21881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-01-20-0018-R"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-11"; volume: "33"; issue: "11"; pages: 
            "1286-1298"
COMMENT     Sequence Label: pBW_0004
FEATURES             Location/Qualifiers
     source          1..21881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    416..440
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        1482..1827
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1770..1790
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    1857..1912
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             1914..2465
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      2488..3195
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(13039..13222)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(13075..13254)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    13378..13402
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      13890..14525
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(15200..15988)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      17085..17673
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     17574..17593
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     17827..17844
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(19033..20181)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     mobile_element  20806..21573
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     oriT            21633..21742
                     /note="incP origin of transfer"
ORIGIN
        1 gtcgacgatc gtcaacgttc acttctaaag aaatagcgcc actcagcttc ctcagcggct
       61 ttatccagcg atttcctatt atgtcggcat agttctcaag atcgacagcc tgtcacggtt
      121 aagcgagaaa tgaataagaa ggctgataat tcggatctct gcgagggaga tgatatttga
      181 tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc
      241 cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca
      301 aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg
      361 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca
      421 ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt
      481 ttttaatgta ctgaattcca atcccacaaa aatctgagct taacagcaca gttgctcctc
      541 tcagagcaga atcgggtatt caacaccctc atatcaacta ctacgttgtg tataacggtc
      601 cacatgccgg tatatacgat gactggggtt gtacaaaggc ggcaacaaac ggcgttcccg
      661 gagttgcaca caagaaattt gccactatta cagaggcaag agcagcagct gacgcgtaca
      721 caacaagtca gcaaacagac aggttgaact tcatccccaa aggagaagct caactcaagc
      781 ccaagagctt tgctaaggcc ctaacaagcc caccaaagca aaaagcccac tggctcacgc
      841 taggaaccaa aaggcccagc agtgatccag ccccaaaaga gatctccttt gccccggaga
      901 ttacaatgga cgatttcctc tatctttacg atctaggaag gaagttcgaa ggtgaaggtg
      961 acgacactat gttcaccact gataatgaga aggttagcct cttcaatttc agaaagaatg
     1021 ctgacccaca gatggttaga gaggcctacg cagcaggtct catcaagacg atctacccga
     1081 gtaacaatct ccaggagatc aaataccttc ccaagaaggt taaagatgca gtcaaaagat
     1141 tcaggactaa ttgcatcaag aacacagaga aagacatatt tctcaagatc agaagtacta
     1201 ttccagtatg gacgattcaa ggcttgcttc ataaaccaag gcaagtaata gagattggag
     1261 tctctaaaaa ggtagttcct actgaatcta aggccatgca tggagtctaa gattcaaatc
     1321 gaggatctaa cagaactcgc cgtgaagact ggcgaacagt tcatacagag tcttttacga
     1381 ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacactct ggtctactcc
     1441 aaaaatgtca aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg
     1501 ataatttcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg
     1561 acagtagaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggctatc
     1621 attcaagatc tctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
     1681 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgacatctcc
     1741 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
     1801 ggaagttcat ttcatttgga gaggacacgc tcgagtataa gagctctatt tttacaacaa
     1861 ttaccaacaa caacaaacaa caaacaacat tacaattact atttacaatt accatgagcc
     1921 cagaacgacg cccggccgac atccgccgtg ccaccgaggc ggacatgccg gcggtctgca
     1981 ccatcgtcaa ccactacatc gagacaagca cggtcaactt ccgtaccgag ccgcaggaac
     2041 cgcaggagtg gacggacgac ctcgtccgtc tgcgggagcg ctatccctgg ctcgtcgccg
     2101 aggtggacgg cgaggtcgcc ggcatcgcct acgcgggccc ctggaaggca cgcaacgcct
     2161 acgactggac ggccgagtca accgtgtacg tctccccccg ccaccagcgg acgggactgg
     2221 gctccacgct ctacacccac ctgctgaagt ccctggaggc acagggcttc aagagcgtgg
     2281 tcgctgtcat cgggctgccc aacgacccga gcgtgcgcat gcacgaggcg ctcggatatg
     2341 ccccccgcgg catgctgcgg gcggccggct tcaagcacgg gaactggcat gacgtgggtt
     2401 tctggcagct ggacttcagc ctgccggtac cgccccgtcc ggtcctgccc gtcaccgaga
     2461 tttgatgacc cgggggatcc tagagtcctg ctttaatgag atatgcgaga cgcctatgat
     2521 cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc
     2581 tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt tactatcgta
     2641 tttttatgaa taatattctc cgttcaattt actgattgta ccctactact tatatgtaca
     2701 atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc tatgatagag
     2761 cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa aaaagcggca
     2821 gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca aaagtgcccc
     2881 aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg aagggaactc
     2941 cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg ccgtccgctc
     3001 taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt aaccgacttg
     3061 ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg aagtgcaggt
     3121 caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt gcgacaacat
     3181 gtcgaggctc agcaggacct gcaggcatgc aagctctaga gcggccgctt taagagtgtt
     3241 gaggatgtgc ttacttaaat ttcaaaaggg ctatttaggg ataatatttg gtgaagcatg
     3301 gctggtggac tgaccatagg tacccactga ataaacatat cagcccaaag gcagagggta
     3361 tccctccctg taatacgtac tagtattcga ggagcgtgct aaagtagtat cctctcttga
     3421 tgctcctcgt ctccctccac tgagtactcc cttcctctcc tcattctcat ctcagtcctc
     3481 tcctcctccc tcgaataaga tagatccgcc cttgttgctg atctcatctc gcctcctatt
     3541 ggcctcgccc ctgccaactg actgctctat tggtgaccgt ggactttttg ggtatatata
     3601 tttcctgtcg atttgtttcc acttaaagta gcaatgcttg gatctaaaac ttattacttc
     3661 tcctgcttca gattctgatc catttgtgtt gattgaactc agggtgggat cgtggggctc
     3721 tgtatctatc taggatggat attccaagca atttactctc ttgagtgctg ttggtcttcc
     3781 tctgctcctc gtctccgctt cctctcagtc tcctccattg cgctgcctgg aacagatcga
     3841 tccgccttgt tgctgatctc atccatcctc cgtggagatt tcgggtggca gtttccagtg
     3901 actattcaga gcaccatact agtggcctgg taaaaatttc cctcccatct ttcttgctat
     3961 tctcttctta ttaatctgct gatttgcttg atggtaagtt cttacttatt cttccagatc
     4021 gagtgaagcg acatccgccg ccgtcttgtc ccctacgacg ccgctgtgtt ctgctctgtc
     4081 ctcctctgcc ggtgaagcat caacggtgac ggccatccgg tgttgacgcc gcggctgtgt
     4141 tctcctctgc cgtgaagtat caaaggtggg tttggatgga tatcacagtt tggattggca
     4201 actctgttct atagctcgtt gaaattaaag cttcacacag ttttgattgg attacagctc
     4261 cccatattca tctattttgc accatgcatc gaatccactg acctccacac gctcacggat
     4321 ctggcctctg gtgcccacac gctcacggat ccgacagagt gctcccttcc tatcatctca
     4381 gtcctcatct cctggtcgat ccggcttctt gtttggtgag ctcgtccaac ctccgagctc
     4441 gcccctgaca cctgtggagt ggaggtatat ttctttccag ctgaagtacg cttggatcta
     4501 aaaacttacg tctcctgctt cactgcttgc ttgggagtct gatcgatttg tgttggttga
     4561 atcatttggg gtggcagtgg agcagctagc aagaatcatg gagtttccaa tcattattca
     4621 gagcaccacg catggaatcc actgacctgg taactttgtc tcatctttct tgctactgaa
     4681 atcttccaca tccccacctt ccttgttcat ctgctgattt cccagattat acttccagat
     4741 tgagtgaagc gccgtcttgt cccctcacgg atctggcctc tggtgcccag ccgccgcttt
     4801 cgcttcgttg tccgacgagg ccaccgccgc tgtgttctgc tctgccgtga agcacaaaaa
     4861 ggtggggtct tcctctgctc tttctccact gacggcgatc cgaccttgac acccgtggcc
     4921 gtggcagtgg ggtcttcctc tgctctgttt ttttaccagt tattggatct aaaaagggat
     4981 ctaaaagtta ttacttctcc tgcttgagat tctgaccgat ttgtgttgat tgaatccagg
     5041 gtggggtcgt ggggctctgt atctatctag tacggagtac cactgtattc caagcaattt
     5101 actctcttga ctgctgttgg acttcctctg tccccgcttc cattgtgctg ccttgaacag
     5161 atccatccac cttgttgcta atctcatcca acctccgggt atgtttcttg tcgatttgtt
     5221 tccagtaaag tagcaatgct tgggtctaaa aagtcattgc ttctcctgct tgacgagtct
     5281 cattgatttg cccagtcatt attcagagca ccatgcatcc aaggcgctga cccggctata
     5341 agtttttatt acagggccgc cgtgttgtcc ccatcgagca aagcgtttaa tccactgacc
     5401 cgcttcgttg tccgcacagc gtgaccatcc gacgaagacg ccgccgttgt gttctgctct
     5461 cctctgaagc atcaaaaggt gggcttggtc cagatcccca cattcatcga tttggcttgc
     5521 caactcagtt ccaccagagc ttggattgtg ttacagatcc ctaaattcat cgatttgcag
     5581 gtttcagctg attgatcaag agagctctca atgatggcgg aagttctggt gagcgcatcc
     5641 acaggggcaa tgggttccct cctgcggaag ttgggagcta tgttgacgga tgaatacaag
     5701 ctgctcaaga acgtccgtgg ggacatcaag ttcctcaagg acgagctgga ggtcatgtgc
     5761 gcgttcctcc tcaagatgtc agatgtggag gagcctgacg aaccaacaaa gctgcgtgtg
     5821 acggcggtgc gggagatgtc ctataagata gaggacaaca tcgacaagtt catggtcctc
     5881 gtggaacacg agtcctcctg ctccgaggcg gctcatggcg tcgccaagct catggacaag
     5941 tgcaagaact tgctgccgga catcaagacc cgccgcagga tcgccaagga ggtcaaagac
     6001 atcaagaaag aaattaagga tgtcagcgac agattttcaa ggtaccagat cttgttctct
     6061 ttacagtact attagtacct accatcagat ttgtgaccga tttgttgcca ctcaaggtac
     6121 aagattgacg agtcctcctc ctctatgccg gcaaaaggca aggtcgaccc tcgacttcgt
     6181 gcagtctaca aagacgccgc agagctcgtc ggaattgatg gaccaaagga tgaacttgtc
     6241 aagtggctga atgagaaaga gggccaatca ctgaaatctg tctctattgt tggatatgga
     6301 gggttgggca agaccacgct cgccaaccag atccgggtca accttggagc gaccttcgac
     6361 tgcggggctt ttgtctcgat ttcacgcaag cctgacatga aggcgatatt aagatctata
     6421 ttatcgcaaa tcaccaagaa agacgaggct tgctctagat tagatgatat acaactcatc
     6481 atcgacaaga ttcgagaatt cctccaagac acaaggtatg tatgtgagat ctactacgaa
     6541 gtaacattgc ttaattagct catgcataat ccgttgccta gaattttgct aatgagattg
     6601 ctttgtgtga tttactttcg tcctcaactt gtatgactgc gcttaaactt tcaagtcatt
     6661 gtttcttgca acttatgtcg atctgttcac taattaagat atagctaaaa tgtgtttgcc
     6721 aatttctgtc gccacacatt ttctaatttg attgccacag gtttgttaaa cttgcggcta
     6781 tgttctgttt attaaataat tgtgacatgg cgtcagtcaa aacaataaag tacatgcaat
     6841 tccctctctt ttgtacaaga taaatatccg cacgcactac atgactgcaa atatatctat
     6901 cttctaagga cccaaagcac ttgcatgtgc agaccattgt taccggatat gcagtacatt
     6961 aggtttgtga ttagccactt taagaacata gggtttctag aggctataaa cctacggtta
     7021 tcagtattat aatcttgaat ggatatcaaa catccgttga agaatctatg cccataacac
     7081 aaaaagagaa aagggaagaa ttaatcatag tcagatcgga ccagcctgac tagcgtaaca
     7141 taatgacaac agtaggttgt tgcgtctttc ctggtttcgt agcaatttgc atgacgaatt
     7201 gaggcgcaga gccaccactc aatatgtggt cctaccaatc atgaggaaat aacttgttac
     7261 caaaatgaca aaagaaaata ggtagtcccc atcattgttt caatatctat cctcttgcga
     7321 acatgctatt ggcagtggca aaacattgta ccttgccact ggcaacagct gcctttactc
     7381 ggcctgagca ttcatatttt tttcctcttg gttgttaaat cttgctattt ttcgcttgcc
     7441 ttctatggca atcttttttt ttcttatatt gccaccaaat tgtatctaag agcaactcca
     7501 acacgccgac gcaatttttg tccatttaga tcggccaagc ggacggggtg tctggttttg
     7561 cctttgggtg gtgcgcctac gcattagtcc gcgtgggcat ttaaaaaact taaaaagctt
     7621 aaaataatta aatataaata aaaatgcata ttaaagcccg tcaaagtcca cataattaaa
     7681 cataatacat taatatatta ataaaaaaca gccgccccag cccgcagccc taggcgtctt
     7741 cgtcggagga gtgggtgagg tcgaagaatg ccggcagctc agcccacggg aaggcctgct
     7801 ggtgggcagc cagcaccgcc tgctccgctg tctgcggcgg cggcggccct acctggcacc
     7861 gcgcacgcct cctcctcgcg ctcctgccgc tcctcctcct catcgcacgc ccaacgaagt
     7921 tttggcgctc ctccccctcg ggttcccgct gcatgcggac ctggccgtcg taccgctcct
     7981 cctccagcgt gcggtccttc cacgccacga ggtaggccgc ctcctcctcc atcacgcaca
     8041 gccaaatggg cggcttgctc atccgcttgc agacgatgcc ctgccattgg taaacgccgg
     8101 tgggcgcggg tggtgctagc gctggtgcag gcggtgacga tgtggctggt ggtcccggca
     8161 ggtagacact atagctggcg gccgatagct gcagcgtgtc atcgaggcca gaccactagg
     8221 agtcctcgca ctaagactcc tcggtgacgt gatgggcccc ctcctccaat gccttgagga
     8281 acgccgcctc ctcctcaacc tactatttga ccaccggtgg cggcggtgga gggcaggctt
     8341 cgagctggac ggcgtcgcgg aggcgcacat ggtgctcgat ggtgaaccag acttctcagt
     8401 taggggagtc tggcctcgat gggtggagtc gcgacgctgc tctggcgtca ggagagcacg
     8461 ccgggcccga atctcgttgg cgcgcgcacg ctcggactgt ggcaccgccg gcactggaat
     8521 gtggtggggg tcgaggtgcc agccgtgggg gaagttcacg tccggccacg gcagtgagac
     8581 gcggtactcc cggtggtact aactacgcgt ggtgcaccgg gatgtactgg tggtcccgtg
     8641 gcgctgccgc cctcccatcc cgcagcgccg acaccaatgc gaaccccttc gctgccggcc
     8701 tcaccggccc ttgcaacgag ccggcctcgt ggtcgtgctt gccttgccct tacctttgcc
     8761 aaagaaaccc atgacggcta gggttttctg tcaccggcga gggagcagat gtggactaga
     8821 gtgggaggag aagtggatga ggcccgcccc gccgcaccct tgcaattaaa aagggcgtga
     8881 tccggacgtt tctagtcact gccatgcggg cccgaggtag gtgggcgcca ttgactacgt
     8941 ctgtggtggt agttggctgg cagacacgcc agcccgaggc gcacaccgag cggacgcgca
     9001 acacgtccgt ctcgtgttcg tgtgaacgca aatcaggcac aaatttgggc tggaaatgcg
     9061 tctacatgga tgccgaacgg acgtgatttg ggtttgtgtc cgcacgttgg gccggcgttt
     9121 ttgtgccttt cgaccctaat ggacacacac ggaccaaatc cgttggcgtg ttagagttgc
     9181 ttgtacctag tactactatg tgtggagttg ttatctttga gtttctcccc catctgtagc
     9241 tgtgtattat tgtgagatgc tctcaggatt tcacttattt tagataatga caactttctt
     9301 gatgctagac aattacatca aggtgtatta tatctaggat gcacacagat gtgagattgt
     9361 ttcaggattt taggttactt ggtaggtgtt actataaata tctaatgagc tctaatgtgt
     9421 atgttcatga aactgtgttg caggtacttt atcataattg atgatatatg ggagttagga
     9481 acatgggaaa ctttgaaatg tgcatttgtc aagaatacat tgggcagcag aataattatc
     9541 acaacacgta tagttgatgt tgccaaatct tgttctcctt ctagtgaaga tcttgtctat
     9601 gagatgaaac cactcagtga ggctgactcg aagaaattgt ttttcaagag gatatttggc
     9661 tgtgaagaaa gctgtcctga tagcttgaaa gaagctgcca atgatatttt gaaaaaatgc
     9721 cgtggcctac ctctggccat caatgccatt tctagcgtgt tggtcacaac aagggaaaca
     9781 aaagaagagt gggatcgggt gcgacattca atacgctctt caaaggtcaa aagtgatata
     9841 atagaaacca tgaattacat attgtctctt agttattttg atcttcccca ccacctaaga
     9901 agctgcttat tgtacctggc tctgtttcct gaagaccaat tgattggaag gaagcggtta
     9961 gtacgcagat ggatttctga aggctttatc catggagaaa gtggacaaga tctcatggaa
    10021 ttaggagagg aatattttca ccagcttgtg aacagaagtc taatacagcc cggcaacata
    10081 gggtatgacg gcaaggcaat gtattgccga gtccatgaca ccatccttga tttcctaatt
    10141 gacaaatcct ctgaagagaa catgtgtact gtcctaaaga aacaatgcaa gcctaatggc
    10201 atagttcgtc ggctctctct aatggggaat gaggatgaag aaatcgtgga gcaattggat
    10261 ctgtcacacg ctcggtcaat cactgctttt ggggatatca agctattgcc ttcgcttggg
    10321 aggtcaaaat gcttgcgcgt gctagacttg caagactgcg atcagttgga aaatcatcat
    10381 atcaaggata ttgaaagact ctatcagcta agatacttgg atatctcttc tacaggaatc
    10441 acggagcttc ccaggcaaat tggggagttg ctgtatctag agacgctagt tgcctatgga
    10501 ttacgtgagc ttcctgaaag cacaagtcgg ctacaacgac tggcacgttt gtttgtttac
    10561 tctggttgta agcttccagg tgggttggga aacttgataa acttgcaaga gcttgactgc
    10621 gttgatgcct tgcatttgaa gcatgtggaa gagctcggca aactaacaaa tctgagaaag
    10681 ctgagtatta aattggacac tggtgggatt gaaggcaaca aattagaaga gtccaaggaa
    10741 aagctggtgt cctctctctg taaactggac gaatgcggcc tcctttccct gagtattgat
    10801 tattatctga gagaaaagga tggagaagag ccgttcctcc ctgctttggg ttgtatccag
    10861 gaggtttttg tatatggaca agatatcagc cggattagca gatggctcgc gtcacttccc
    10921 aacctacacg ggttgctttt ggacaatccg aagatagagc agcaggatat tgagatgatt
    10981 ggattgatac ccaatctgat cgatttgaca ctgtatttac gcataacaga cgacgctggg
    11041 cggctcatca tcaagcgtga aggatttcaa cagttgcaga ggtttgagct ttcccgtatt
    11101 cgtatggggg atttaatgtt tgaaccgggg gctatgccga ggctcaagga gcttatactc
    11161 taccacttca tcgaaaagcc caaatctggt gcggtcgatt tcgactttgg catccaacac
    11221 ctctccagcc ttgctcgcct cactgtcggc ttattatgcg ttggctcgac ggcggcggaa
    11281 gtagaggctg cggaggatgc tttcaagagc atggccgagg cgaaccccaa ccgtccaata
    11341 ctggagatga caagacttta tccgcagcat atgttacaag acgagcaaat aggtatgaca
    11401 ggctcaggta ctattaacta gtttgtactt ttgtcttggt gagcagtaga tcaaaagatg
    11461 tgcatatgct tcaatctttt ttccggtaat ctatttcagc tacaacgccg gccgtcaagt
    11521 cctaggcttc tgacgttgct tcaggtgtgc tctcaatcta tacatattta ctcatcatat
    11581 tattctccac ctttttttcc atctctagag ctcagcttgt cattgcattg ttcaattgtg
    11641 cttctctagc agctacggaa attctgaaca ggcaaaggta attttacaga agtttctttg
    11701 gcataagtat ctgagaaaaa tgcacttggc acaatttacc ttttcgcagt agaaaagagt
    11761 cagtggatca taccttctta attgttcgat tgctcgtaat acttgggggc actctatgat
    11821 tgctgtgtgc ttttagtttt gatatagttc ctgttagcat ggacagactg tgtgattggt
    11881 tcatgaattt cagggtggtt ctaatgagga aatgatgctt gcttcagaaa tagtttccat
    11941 ttgtacctaa gctgtgtagt tggtaaagtt gcattttgga tgttctgttg gtatagctta
    12001 cagaagaaag acttgcgttg tgcaggtacc aggggcgcag gttactgcta gtggcgtctg
    12061 agatgtttca taaacgttgg gtgggcaccg gtcatgcaaa aaattgaagg ataaaaaggt
    12121 tccgagaggt tgtgtgatgt gctcctttta aatggcagtc gcttgcataa agagatgttg
    12181 cttggttttt tagtaaagcg ttgtagtaag ttgctctgtt tcggctgaga gccatcgttg
    12241 ttgctgtctg ttacatcgaa tggctggctc atgtcatgtt gatgtttttt ttatctgaac
    12301 cggacaaacc tgagggtgta gtatcatgtt tgtaatgact gaaattctgg gctcacccga
    12361 gctccttaat tcgtaaacaa ctgcgactca ttgttggatt ccttacttca gaaaatattt
    12421 acttgctctg gctcatacag ctctgcatgt tttgtagtgg tttgtcgctg tttaggtgct
    12481 gcttcagact cctggtacct ttggccgctg ctgctactgc atgagctttg tatggcattg
    12541 ttagtatagt ttggtgtcat tgggttttcg ccccatggtg agtgtccatt gcgcgcactc
    12601 gcagcggttt cctggcgata agttgaactt gatctttctc tttcccacct cctaaacgtc
    12661 tattcagact tgtgtatatt tgcattacgg ggattacccg agttcaaaaa taaatattat
    12721 ttactagttc tgccaatgtg tttccgttac atacaaataa aagggtgctt ccagctacaa
    12781 gtatattttt gagaagggcc attgcaaaac gaaatgaagc ccaaattcta ccaacatgtt
    12841 tctgtcacat atacattaaa aaaacggtgt aaatgtcccg tcaaaaaagg gtgtatgtag
    12901 gaaaaggagt tcgacgccgc cagctccctc ctaacgctca gattgtcttc gcggccgcca
    12961 ccgcggctga gtggctcctt caagcttgcg gatccgggcg gaaataggta aagaagttgc
    13021 ggataaggta attgccattg cagattattt ggattgagag tgaatatgag actctaattg
    13081 gataccgagg ggaatttatg gaacgtcagt ggagcatttt tgacaagaaa tatttgctag
    13141 ctgatagtga ccttaggcga cttttgaacg cgcaataatg gtttctgacg tatgtgctta
    13201 gctcattaaa ctccagaaac ccgcggctga gtggctcctt caacgttgcg gttctgtcag
    13261 ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt gactccctta
    13321 attctccgct catgatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga
    13381 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt
    13441 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
    13501 ttcccctcgg gagtgcttgg cattccgtgc gataatgact tctgttcaac cacccaaacg
    13561 tcggaaagcc tgacgacgga gcagcattcc aaaaagatcc cttggctcgt ctgggtcggc
    13621 tagaaggtcg agtgggctgc tgtggcttga tccctcaacg cggtcgcgga cgtagcgcag
    13681 cgccgaaaaa tcctcgatcg caaatccgac gctgtcgaaa agcgtgatct gcttgtcgct
    13741 ctttcggccg acgtcctggc cagtcatcac gcgccaaagt tccgtcacag gatgatctgg
    13801 cgcgagttgc tggatctcgc cttcaatccg ggtctgtggc gggaactcca cgaaaatatc
    13861 cgaacgcagc aagatcgtcg accctttccg acgctcaccg ggctggttgc cctcgccgct
    13921 gggctggcgg ccgtctatgg ccctgcaaac gcgccagaaa cgccgtcgaa gccgtgtgcg
    13981 agacaccgcg gccggccgcc ggcgttgtgg atacctcgcg gaaaacttgg ccctcactga
    14041 cagatgaggg gcggacgttg acacttgagg ggccgactca cccggcgcgg cgttgacaga
    14101 tgaggggcag gctcgatttc ggccggcgac gtggagctgg ccagcctcgc aaatcggcga
    14161 aaacgcctga ttttacgcga gtttcccaca gatgatgtgg acaagcctgg ggataagtgc
    14221 cctgcggtat tgacacttga ggggcgcgac tactgacaga tgaggggcgc gatccttgac
    14281 acttgagggg cagagtgctg acagatgagg ggcgcaccta ttgacatttg aggggctgtc
    14341 cacaggcaga aaatccagca tttgcaaggg tttccgcccg tttttcggcc accgctaacc
    14401 tgtcttttaa cctgctttta aaccaatatt tataaacctt gtttttaacc agggctgcgc
    14461 cctgtgcgcg tgaccgcgca cgccgaaggg gggtgccccc ccttctcgaa ccctcccggc
    14521 ccgctaacgc gggcctccca tccccccagg ggctgcgccc ctcggccgcg aacggcctca
    14581 ccccaaaaat ggcagccaag ctcctaacat tttattagag agcaggctag ttgcttagat
    14641 acatgatctt caggccgtta tctgtcaggg caagcgaaaa ttggccattt atgacgacca
    14701 atgccccgca gaagctccca tctttgccgc catagacgcc gcgcccccct tttggggtgt
    14761 agaacatcct tttgccagat gtggaaaaga agttcgttgt cccattgttg gcaatgacgt
    14821 agtagccggc gaaagtgcga gacccatttg cgctatatat aagcctacga tttccgttgc
    14881 gactattgtc gtaattggat gaactattat cgtagttgct ctcagagttg tcgtaatttg
    14941 atggactatt gtcgtaattg cttatggagt tgtcgtagtt gcttggagaa atgtcgtagt
    15001 tggatgggga gtagtcatag ggaagacgag cttcatccac taaaacaatt ggcaggtcag
    15061 caagtgcctg ccccgatgcc atcgcaagta cgaggcttag aaccaccttc aacagatcgc
    15121 gcatagtctt ccccagctct ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc
    15181 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
    15241 gaacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttgt ccaagataag
    15301 cctgcctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt
    15361 cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca
    15421 acgtaagcac tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta
    15481 aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg
    15541 ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat
    15601 caatgtcgat cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc
    15661 caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa
    15721 tggtgacttc tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa
    15781 ggtcgttgat caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca
    15841 aatcaatatc actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg
    15901 ccagcaacgt cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg
    15961 atacttcggc gatcaccgct tccctcatga tgtttaactc ctgaattaag ccgcgccgcg
    16021 aagcggtgtc ggcttgaatg aattgttagg cgtcatcctg tgctcccgag aaccagtacc
    16081 agtacatcgc tgtttcgttc gagacttgag gtctagtttt atacgtgaac aggtcaatgc
    16141 cgccgagagt aaagccacat tttgcgtaca aattgcaggc aggtacattg ttcgtttgtg
    16201 tctctaatcg tatgccaagg agctgtctgc ttagtgccca ctttttcgca aattcgatga
    16261 gactgtgcgc gactcctttg cctcggtgcg tgtgcgacac aacaatgtgt tcgatagagg
    16321 ctagatcgtt ccatgttgag ttgagttcaa tcttcccgac aagctcttgg tcgatgaatg
    16381 cgccatagca agcagagtct tcatcagagt catcatccga gatgtaatcc ttccggtagg
    16441 ggctcacact tctggtagat agttcaaagc cttggtcgga taggtgcaca tcgaacactt
    16501 cacgaacaat gaaatggttc tcagcatcca atgtttccgc cacctgctca gggatcaccg
    16561 aaatcttcat atgacgccta acgcctggca cagcggatcg caaacctggc gcggcttttg
    16621 gcacaaaagg cgtgacaggt ttgcgaatcc gttgctgcca cttgtttaat agactggatg
    16681 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
    16741 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
    16801 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
    16861 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
    16921 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    16981 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    17041 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgata tccttttttt
    17101 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    17161 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    17221 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    17281 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    17341 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    17401 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    17461 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    17521 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    17581 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    17641 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    17701 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    17761 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    17821 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
    17881 acgcatctgt gcggtatttc acaccgcata tgaagatcgg cggcaatagc ttcttagcgc
    17941 catcccggct gagaaagccc agtaaggaaa caactgtagg ttcgagtcgc gagatccccc
    18001 ggaaccaaag gaagtaggtt aaacccgctc cgatcaggcc gagccacgcc aggccgagaa
    18061 cattggttcc tgtaggcatc gggattggcg gatcaaacac taaagctact ggaacgagca
    18121 gaagtcctcc ggccgccagt tgccaggcgg taaaggtgag cagaggcacg ggaggttgcc
    18181 acttgcgggt cagcacggtt ccgaacgcca tggaaaccgc ccccgccagg cccgctgcga
    18241 cgccgacagg atctagcgct gcgtttggtg tcaacaccaa cagcgccacg cccgcagttc
    18301 cgcaaatagc ccccaggacc gccatcaatc gtatcgggct acctagcaga gcggcagaga
    18361 tgaacacgac catcagcggc tgcacagcgc ctaccgtcgc cgcgaccccg cccggcaggc
    18421 ggtagaccga aataaacaac aagctccaga atagcgaaat attaagtgcg ccgaggatga
    18481 agatgcgcat ccaccagatt cccgttggaa tctgtcggac gatcatcacg agcaataaac
    18541 ccgccggcaa cgcccgcagc agcataccgg cgacccctcg gcctcgctgt tcgggctcca
    18601 cgaaaacgcc ggacagatgc gccttgtgag cgtccttggg gccgtcctcc tgtttgaaga
    18661 ccgacagccc aatgatctcg ccgtcgatgt aggcgccgaa tgccacggca tctcgcaacc
    18721 gttcagcgaa cgcctccatg ggctttttct cctcgtgctc gtaaacggac ccgaacatct
    18781 ctggagcttt cttcagggcc gacaatcgga tctcgcggaa atcctgcacg tcggccgctc
    18841 caagccgtcg aatctgagcc ttaatcacaa ttgtcaattt taatcctctg tttatcggca
    18901 gttcgtagag cgcgccgtgc gtcccgagcg atactgagcg aagcaagtgc gtcgagcagt
    18961 gcccgcttgt tcctgaaatg ccagtaaagc gctggctgct gaacccccag ccggaactga
    19021 ccccacaagg ccctagcgtt tgcaatgcac caggtcatca ttgacccagg cgtgttccac
    19081 caggccgctg cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac
    19141 gcgggtggaa tccgatccgc acatgaggcg gaaggtttcc agcttgagcg ggtacggctc
    19201 ccggtgcgag ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct tgcggtactt
    19261 ctcccatatg aatttcgtgt agtggtcgcc agcaaacagc acgacgattt cctcgtcgat
    19321 caggacctgg caacgggacg ttttcttgcc acggtccagg acgcggaagc ggtgcagcag
    19381 cgacaccgat tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag
    19441 gcgcgacagg cattcctcgg ccttcgtgta ataccggcca ttgatcgacc agcccaggtc
    19501 ctggcaaagc tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta
    19561 ctccaacacc tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta
    19621 ggtgatcttc acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg
    19681 gattttcttg ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg
    19741 catcgtgtcc ggccacggcg caatatcgaa caaggaaagc tgcatttcct tgatctgctg
    19801 cttcgtgtgt ttcagcaacg cggcctgctt ggcctcgctg acctgttttg ccaggtcctc
    19861 gccggcggtt tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt
    19921 cgccaaacct gccgcctcct gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc
    19981 gggcagggca gggggagcca gttgcacgct gtcgcgctcg atcttggccg tagcttgctg
    20041 gaccatcgag ccgacggact ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc
    20101 gatggtttcg gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt
    20161 ccggtcaaac gtccgattca ttcaccctcc ttgcgggatt gccccgactc acgccggggc
    20221 aatgtgccct tattcctgat ttgacccgcc tggtgccttg gtgtccagat aatccacctt
    20281 atcggcaatg aagtcggtcc cgtagaccgt ctggccgtcc ttctcgtact tggtattccg
    20341 aatcttgccc tgcacgaata ccagctccgc gaagtcgctc ttcttgatgg agcgcatggg
    20401 gacgtgcttg gcaatcacgc gcaccccccg gccgttttag cggctaaaaa agtcatggct
    20461 ctgccctcgg gcggaccacg cccatcatga ccttgccaag ctcgtcctgc ttctcttcga
    20521 tcttcgccag cagggcgagg atcgtggcat caccgaaccg cgccgtgcgc gggtcgtcgg
    20581 tgagccagag tttcagcagg ccgcccaggc ggcccaggtc gccattgatg cgggccagct
    20641 cgcggacgtg ctcatagtcc acgacgcccg tgattttgta gccctggccg acggccagca
    20701 ggtaggccga caggctcatg ccggccgccg ccgccttttc ctcaatcgct cttcgttcgt
    20761 ctggaaggca gtacaccttg ataggtgggc tgcccttcct ggttgggtaa tgactccaac
    20821 ttattgatag tgttttatgt tcagataatg cccgatgact ttgtcatgca gctccaccga
    20881 ttttgagaac gacagcgact tccgtcccag ccgtgccagg tgctgcctca gattcaggtt
    20941 atgccgctca attcgctgcg tatatcgctt gctgattacg tgcagctttc ccttcaggcg
    21001 ggattcatac agcggccagc catccgtcat ccatatcacc acgtcaaagg gtgacagcag
    21061 gctcataaga cgccccagcg tcgccatagt gcgttcaccg aatacgtgcg caacaaccgt
    21121 cttccggaga ctgtcatacg cgtaaaacag ccagcgctgg cgcgatttag ccccgacata
    21181 gccccactgt tcgtccattt ccgcgcagac gatgacgtca ctgcccggct gtatgcgcga
    21241 ggttaccgac tgcggcctga gttttttaag tgacgtaaaa tcgtgttgag gccaacgccc
    21301 ataatgcggg ctgttgcccg gcatccaacg ccattcatgg ccatatcaat gattttctgg
    21361 tgcgtaccgg gttgagaagc ggtgtaagtg aactgcagtt gccatgtttt acggcagtga
    21421 gagcagagat agcgctgatg tccggcggtg cttttgccgt tacgcaccac cccgtcagta
    21481 gctgaacagg agggacagct gatagaaaca gaagccactg gagcacctca aaaacaccat
    21541 catacactaa atcagtaagt tggcagcatc acccctggtt ggcttggttt catcagccat
    21601 ccgcttgccc tcatctgtta cgccggcggt agccggccag cctcgcagag caggattccc
    21661 gttgagcacc gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg
    21721 gcctacttca cctatcctgc ccggctgacg ccgttggata caccaaggaa agtctacacg
    21781 aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc
    21841 tataatgacc ccgaagcagg gttatgcagc ggaaaagatc c
//
