LOCUS       sequence-200228-       21888 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector containing Sr22 PI573523 allele driven by Sr33 
            promoter, Sr33 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21888)
  AUTHORS   Md Hatta MA, Ghosh S, Athiyannan N, Richardson T, Steuernagel B, Yu 
            G, Rouse MN, Ayliffe M, Lagudah ES, Radhakrishnan GV, Periyannan SK,
            Wulff BBH
  TITLE     Extensive Genetic Variation at the Sr22 Wheat Stem Rust Resistance 
            Gene Locus in the Grasses Revealed Through Evolutionary Genomics and
            Functional Analyses.
  JOURNAL   Mol Plant Microbe Interact. 2020 Nov;33(11):1286-1298. doi: 
            10.1094/MPMI-01-20-0018-R. Epub 2020 Oct 1.
  PUBMED    32779520
REFERENCE   2  (bases 1 to 21888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-01-20-0018-R"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-11"; volume: "33"; issue: "11"; pages: 
            "1286-1298"
COMMENT     Sequence Label: pBW_0007
FEATURES             Location/Qualifiers
     source          1..21888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    416..440
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        1482..1827
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1770..1790
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    1857..1912
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             1914..2465
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      2488..3195
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(13046..13229)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(13082..13261)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    13385..13409
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      13897..14532
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(15207..15995)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      17092..17680
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     17581..17600
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     17834..17851
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(19040..20188)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     mobile_element  20813..21580
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     oriT            21640..21749
                     /note="incP origin of transfer"
ORIGIN
        1 gtcgacgatc gtcaacgttc acttctaaag aaatagcgcc actcagcttc ctcagcggct
       61 ttatccagcg atttcctatt atgtcggcat agttctcaag atcgacagcc tgtcacggtt
      121 aagcgagaaa tgaataagaa ggctgataat tcggatctct gcgagggaga tgatatttga
      181 tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc
      241 cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca
      301 aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg
      361 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca
      421 ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt
      481 ttttaatgta ctgaattcca atcccacaaa aatctgagct taacagcaca gttgctcctc
      541 tcagagcaga atcgggtatt caacaccctc atatcaacta ctacgttgtg tataacggtc
      601 cacatgccgg tatatacgat gactggggtt gtacaaaggc ggcaacaaac ggcgttcccg
      661 gagttgcaca caagaaattt gccactatta cagaggcaag agcagcagct gacgcgtaca
      721 caacaagtca gcaaacagac aggttgaact tcatccccaa aggagaagct caactcaagc
      781 ccaagagctt tgctaaggcc ctaacaagcc caccaaagca aaaagcccac tggctcacgc
      841 taggaaccaa aaggcccagc agtgatccag ccccaaaaga gatctccttt gccccggaga
      901 ttacaatgga cgatttcctc tatctttacg atctaggaag gaagttcgaa ggtgaaggtg
      961 acgacactat gttcaccact gataatgaga aggttagcct cttcaatttc agaaagaatg
     1021 ctgacccaca gatggttaga gaggcctacg cagcaggtct catcaagacg atctacccga
     1081 gtaacaatct ccaggagatc aaataccttc ccaagaaggt taaagatgca gtcaaaagat
     1141 tcaggactaa ttgcatcaag aacacagaga aagacatatt tctcaagatc agaagtacta
     1201 ttccagtatg gacgattcaa ggcttgcttc ataaaccaag gcaagtaata gagattggag
     1261 tctctaaaaa ggtagttcct actgaatcta aggccatgca tggagtctaa gattcaaatc
     1321 gaggatctaa cagaactcgc cgtgaagact ggcgaacagt tcatacagag tcttttacga
     1381 ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacactct ggtctactcc
     1441 aaaaatgtca aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg
     1501 ataatttcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg
     1561 acagtagaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggctatc
     1621 attcaagatc tctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
     1681 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgacatctcc
     1741 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
     1801 ggaagttcat ttcatttgga gaggacacgc tcgagtataa gagctctatt tttacaacaa
     1861 ttaccaacaa caacaaacaa caaacaacat tacaattact atttacaatt accatgagcc
     1921 cagaacgacg cccggccgac atccgccgtg ccaccgaggc ggacatgccg gcggtctgca
     1981 ccatcgtcaa ccactacatc gagacaagca cggtcaactt ccgtaccgag ccgcaggaac
     2041 cgcaggagtg gacggacgac ctcgtccgtc tgcgggagcg ctatccctgg ctcgtcgccg
     2101 aggtggacgg cgaggtcgcc ggcatcgcct acgcgggccc ctggaaggca cgcaacgcct
     2161 acgactggac ggccgagtca accgtgtacg tctccccccg ccaccagcgg acgggactgg
     2221 gctccacgct ctacacccac ctgctgaagt ccctggaggc acagggcttc aagagcgtgg
     2281 tcgctgtcat cgggctgccc aacgacccga gcgtgcgcat gcacgaggcg ctcggatatg
     2341 ccccccgcgg catgctgcgg gcggccggct tcaagcacgg gaactggcat gacgtgggtt
     2401 tctggcagct ggacttcagc ctgccggtac cgccccgtcc ggtcctgccc gtcaccgaga
     2461 tttgatgacc cgggggatcc tagagtcctg ctttaatgag atatgcgaga cgcctatgat
     2521 cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc
     2581 tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt tactatcgta
     2641 tttttatgaa taatattctc cgttcaattt actgattgta ccctactact tatatgtaca
     2701 atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc tatgatagag
     2761 cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa aaaagcggca
     2821 gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca aaagtgcccc
     2881 aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg aagggaactc
     2941 cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg ccgtccgctc
     3001 taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt aaccgacttg
     3061 ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg aagtgcaggt
     3121 caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt gcgacaacat
     3181 gtcgaggctc agcaggacct gcaggcatgc aagctctaga gcggccgctt taagagtgtt
     3241 gaggatgtgc ttacttaaat ttcaaaaggg ctatttaggg ataatatttg gtgaagcatg
     3301 gctggtggac tgaccatagg tacccactga ataaacatat cagcccaaag gcagagggta
     3361 tccctccctg taatacgtac tagtattcga ggagcgtgct aaagtagtat cctctcttga
     3421 tgctcctcgt ctccctccac tgagtactcc cttcctctcc tcattctcat ctcagtcctc
     3481 tcctcctccc tcgaataaga tagatccgcc cttgttgctg atctcatctc gcctcctatt
     3541 ggcctcgccc ctgccaactg actgctctat tggtgaccgt ggactttttg ggtatatata
     3601 tttcctgtcg atttgtttcc acttaaagta gcaatgcttg gatctaaaac ttattacttc
     3661 tcctgcttca gattctgatc catttgtgtt gattgaactc agggtgggat cgtggggctc
     3721 tgtatctatc taggatggat attccaagca atttactctc ttgagtgctg ttggtcttcc
     3781 tctgctcctc gtctccgctt cctctcagtc tcctccattg cgctgcctgg aacagatcga
     3841 tccgccttgt tgctgatctc atccatcctc cgtggagatt tcgggtggca gtttccagtg
     3901 actattcaga gcaccatact agtggcctgg taaaaatttc cctcccatct ttcttgctat
     3961 tctcttctta ttaatctgct gatttgcttg atggtaagtt cttacttatt cttccagatc
     4021 gagtgaagcg acatccgccg ccgtcttgtc ccctacgacg ccgctgtgtt ctgctctgtc
     4081 ctcctctgcc ggtgaagcat caacggtgac ggccatccgg tgttgacgcc gcggctgtgt
     4141 tctcctctgc cgtgaagtat caaaggtggg tttggatgga tatcacagtt tggattggca
     4201 actctgttct atagctcgtt gaaattaaag cttcacacag ttttgattgg attacagctc
     4261 cccatattca tctattttgc accatgcatc gaatccactg acctccacac gctcacggat
     4321 ctggcctctg gtgcccacac gctcacggat ccgacagagt gctcccttcc tatcatctca
     4381 gtcctcatct cctggtcgat ccggcttctt gtttggtgag ctcgtccaac ctccgagctc
     4441 gcccctgaca cctgtggagt ggaggtatat ttctttccag ctgaagtacg cttggatcta
     4501 aaaacttacg tctcctgctt cactgcttgc ttgggagtct gatcgatttg tgttggttga
     4561 atcatttggg gtggcagtgg agcagctagc aagaatcatg gagtttccaa tcattattca
     4621 gagcaccacg catggaatcc actgacctgg taactttgtc tcatctttct tgctactgaa
     4681 atcttccaca tccccacctt ccttgttcat ctgctgattt cccagattat acttccagat
     4741 tgagtgaagc gccgtcttgt cccctcacgg atctggcctc tggtgcccag ccgccgcttt
     4801 cgcttcgttg tccgacgagg ccaccgccgc tgtgttctgc tctgccgtga agcacaaaaa
     4861 ggtggggtct tcctctgctc tttctccact gacggcgatc cgaccttgac acccgtggcc
     4921 gtggcagtgg ggtcttcctc tgctctgttt ttttaccagt tattggatct aaaaagggat
     4981 ctaaaagtta ttacttctcc tgcttgagat tctgaccgat ttgtgttgat tgaatccagg
     5041 gtggggtcgt ggggctctgt atctatctag tacggagtac cactgtattc caagcaattt
     5101 actctcttga ctgctgttgg acttcctctg tccccgcttc cattgtgctg ccttgaacag
     5161 atccatccac cttgttgcta atctcatcca acctccgggt atgtttcttg tcgatttgtt
     5221 tccagtaaag tagcaatgct tgggtctaaa aagtcattgc ttctcctgct tgacgagtct
     5281 cattgatttg cccagtcatt attcagagca ccatgcatcc aaggcgctga cccggctata
     5341 agtttttatt acagggccgc cgtgttgtcc ccatcgagca aagcgtttaa tccactgacc
     5401 cgcttcgttg tccgcacagc gtgaccatcc gacgaagacg ccgccgttgt gttctgctct
     5461 cctctgaagc atcaaaaggt gggcttggtc cagatcccca cattcatcga tttggcttgc
     5521 caactcagtt ccaccagagc ttggattgtg ttacagatcc ctaaattcat cgatttgcag
     5581 gtttcagctg attgatcaag agagctctca atgatggcgg aagttctggt gagcgcatcc
     5641 acaggcgcaa tgggttccct cctccggaag ttgggagcta tgttgacgga tgaatacaag
     5701 ctgctcaaga acgtccgtgg ggacatcaag ttcctcaagg acgagctgga ggtcatgtgc
     5761 gcgttcctcc tcaagatgtc agatgtggag gagcctgacg aaccaacaaa gctgcgtgtg
     5821 acggcggtgc gggagatgtc ctataagata gaggacaaca tcgacaagtt catggtcctc
     5881 gtggaacacg agtcctcctg ctccgaggcg gctcatggcg tcgccaagct catggacaag
     5941 tgcaagaact tgctgccgga catcaagacc cgccgcagga tcgccaagga ggtcaaagac
     6001 atcaagaaag aaattaagga tgtcagcgac agattttcaa ggtaccagat cttgttctct
     6061 ttacagtact attagtacct accatcagat ttgtgaccga tttgttgcca ctcaaggtac
     6121 aagattgacg agtcctcctc ctctatgccg gcaaaaggca aggtcgaccc tcgacttcgt
     6181 gcagtctaca aagacgccgc agagctcgtc ggaattgatg gaccaaagga tgaacttgtc
     6241 aagtggctga atgagaaaga gggccaatca ctgaaatctg tctctattgt tggatatgga
     6301 gggttgggca agaccacgct cgccaaccag atccgggtca accttggagc gaccttcgac
     6361 tgcggggctt ttgtctcgat ttcacgcaag cctgacatga aggcgatatt aagatctata
     6421 ttatcacaaa tcaccaagaa agacgatgct tgctctagat tagatgatat acaactcatc
     6481 atcgacaaga ttcgagaatt cctccaagac acaaggtatg tatgtgagat ctgctacgaa
     6541 gtaacattgc ttaattagct catgcataat cggttgccta gaattttgct aatgaagatt
     6601 gctttgtgtg atttactttc gtcctcaact tgtatgactg cgcttaaact ttcaagtcat
     6661 tgtttcttgc aacttatgtc gatctgttca ctaattaaga tatagctaaa atgtgtttgc
     6721 cgatttctgt cgccacacat tttctaattt gattgccaca agtttgttaa acttgcggct
     6781 atgttctgtt tattaaataa ttgtgacatg gcgtcagtca aaacaataaa gtacatgcaa
     6841 ttccctctct tttgtacaag ataaatatcc gcacgcacta catgactgca aatatatcta
     6901 tcttctaagg acccaaagca cttgcatgtg cagaccattg ttaccggata tgcagtacat
     6961 taggtttgtg attagccact ttaagaacat agggtttcta gaggctataa acctacggtt
     7021 atcagtatta taatcttgaa tggatatcaa acatccgttg aagaatctat gcccataaca
     7081 caaaaagaga aaagggaaga attaatcata gtcagatcgg accagcctga ctagcgtaac
     7141 ataatgacaa cagtaggttg ttgcgtcttt cctggtttcg tagcaatttg catgacgaat
     7201 tgaggcgcag agccaccact caatatgtgg tcctaccaat catgaggaaa taacttgtta
     7261 ccaaaatgac aaaagaaaat aggtagtccc catcattgtt tcaatatcta tcctcttgcg
     7321 aacatgctat tggcagtggc aaaacattgt accttgccac tggcaacagc tgcctttact
     7381 cggcctgagc attcatattt ttttcctctt ggttgttaaa tcttgctatt tttcgcttgc
     7441 cttctatggc aatctttttt tttcttatat tgccaccaaa ttgtatctaa gagcaactcc
     7501 aacacgccga cgcaattttt gtccatttag atcggccaag cggacggggt gtctggtttt
     7561 gcctttgggt ggtgcgccta cgcattagtc cgcgtgggca tttaaaaaac ttaaaaagct
     7621 taaaataatt aaatataaat aaaaatgcat attaaagccc atcaaagtcc acataattaa
     7681 acataataca ttaatatatt aataaaaaac agccgcccca gcccgcagcc ctaggcgtct
     7741 tcgtcggagg agtgggtgag gtcgaagaat gccggcagct cagcccacgg gaaggcctgc
     7801 tggtgggcag ccagcaccgc ctgctccgct gtctgcggcg gcggcggccc tacctggcac
     7861 cgcgcacgcc tcctcctcgc gctcctgccg ctcctcctcc tcatcgcacg cccaacgaag
     7921 ttttggcgct cctccccctc gggttcccgc tgcatgcgga cctggccgtc gtaccgctcc
     7981 tcctccagcg tgcggtcctt ccacgccacg aggtaggccg cctcctcctc catcacgcac
     8041 agccaaatgg gcggcttgct catccgcttg cagacgatgc cctgccattg gtaaacgccg
     8101 gtgggcgcgg gtggtgctag cgctggtgca ggcggtgacg atgtggctgg tggtcccggc
     8161 aggtagacac tatagttggc ggccgatagc tgcagcgtgt catcgaggcc agaccactag
     8221 gagtcctcgc actaagactc ctcggtgacg tgatgggccc cctcctccaa tgccttgagg
     8281 aacgccgcct cctcctcaac ctactatttg accaccggtg gcggcggtgg agggcaggct
     8341 tcgagctgga cggcgtcgcg gaggcgcaca tggtgctcga tggtgaacca gacttctcag
     8401 ttaggggagt ctggcctcga tgggtggagt cgcgacgctg ctctggcgtc aggagagcac
     8461 gccgggcccg aatctcgttg gcgcgcgcac gctcggactg tggcaccgcc ggcactggaa
     8521 tgtggtgggg gtcgaggtgc cagccgtggg ggaagttcac gtccggccat ggcagtgaga
     8581 cgcggtactc ccggtggtac taactacgcg tggtgcaccg ggatgtactg gtggtcccgt
     8641 ggcgctgccg ccctcccatc ccgcagcgcc gacaccaatg cgaacccctt cgctgccggc
     8701 ctcaccggcc cttgcaacga gccggcctcg tggtcgtgct tgccttgccc ttacctttgc
     8761 caaagaaacc catgacggct agggttttct gtcaccggcg agggagcaga tgtggactag
     8821 agtgggagga gaagtggatg aggcccgccc cgccgcaccc ttgcaattaa aaagggcgtg
     8881 atccggacgt ttctagtcac tgccatgcgg gcccgaggta ggtgggcggc attgactacg
     8941 tctgtggtgg tagttggctg gcagacacgc cagcccgagg cgcacaccga gcggacgcgc
     9001 aacacgtccg tctcgtgttc gtgtgaacgc aaatcaggca caaatttggg ctggaaatgc
     9061 gtctacatgg atgccgaacg gacgtgattt gggtttgtgt ccgcacgttg ggccggcgtt
     9121 tttgtgcctt tcgaccctaa tggacacaca cggaccaaat ccgttggcgt gttagagttg
     9181 cttgtaccta gtactactat gtgtggagtt gttatctttg agtttctccc ccatctgtag
     9241 ctgtgtatta ttgtgagatg ctctcaggat ttcacttatt ttagataatg acaactttct
     9301 tgatgctaga caattacatc aaggtgtatt atatctagga tgcacacaga tgtgagattg
     9361 tttcaggatt ttaggttact tggtaggtgt tactataaat atctaatgag ctctaatgtg
     9421 tatgttcatg aaactgtgtt gcaggtactt tatcataatt gatgatatat gggagttagg
     9481 aacatgggaa actttgaaat gtgcatttgt caagaataca ttgggcagca gaataattat
     9541 cacaacacgt atagttgatg ttgccaaatc ttgttctcct tctagtgaag atcttgtcta
     9601 tgagatgaaa ccactcagtg aggctgactc gaagaaattg tttttcaaga ggatatttgg
     9661 ctgtgaagaa agctgtcctg atagcttgaa agaagctgcc aatgatattt tgaaaaaatg
     9721 ccgtggccta cctctggcca tcaatgccat ttctagcttg ttggtcacaa caagggaaac
     9781 aaaagaagag tgggatcggg tgcgacattc aatacgctct tcaaaggtca aaagtgatat
     9841 aatagaaacc atgaattaca tattgtctct tagttatttt gatcttcccc accacctaag
     9901 aagctgctta ttgtacctgg ctctgtttcc tgaagaccaa ttgattggaa ggaagcggtt
     9961 agtacgcaga tggatttctg aaggctttat ccatggagaa agtggacaag atctcatgga
    10021 attaggagag gaatattttc accagcttgt gaacagaagt ctaatacagc ccggcaacat
    10081 agggtatgac ggcaaggcgg agtattgccg agtccatgac accatccttg atttcctaat
    10141 tgacaaatcc tctgaagaga acatgtgtac tgtcctgaag aaacaatgca agcctaatgg
    10201 catagttcgt cggctctctc taatggggaa tgaggatgaa gaaatcatgg agcaattgga
    10261 tctgtcacac gctcggtcaa tcagtgcttt tggggatatc aagctattgc cttcgcttgg
    10321 gaggtcaaaa tgcttgcgcg tgctagactt gcaaggctgc tatcagttga aaaatcatca
    10381 tatcaaggat attgaaagac tctatcagct gagatacttg gatatctctt ttacaggaat
    10441 cacggagctt cccaggcaaa ttggggagtt gctgtatcta gagacgctag ttagtacatc
    10501 ctatggatta cgtgagcttc ctgaaagcac aagtcggcta caacgactgg cacgtttgtt
    10561 tgttgacagc ggttgtaagc ttccagatgg gttgggaaac ttgataaact tgcaagagct
    10621 tgactgcgtt gatgccttgc agttgaagca tgtggaagag ctcggcaaac taacaaatct
    10681 gagaaagctg aggattaaat tggacactga tgggattgaa ggcaacaaat tagaacagtc
    10741 caaggaaaag ctggtgtcct ctctctgtaa actggacgaa tgcggcctcc tttccctgag
    10801 tatttattat tatctgagag aaaaggatgg agaagagccg ttcctccctg ctttgggttg
    10861 tatccaggag gtttttgtac atggacaaga tatcagccgg attagcagat ggctcgcgtc
    10921 acttcccaac ctacacaggt tgtttttgaa ccaaccgaag atagagcagc aggatattga
    10981 gatgattgga ttgataccca atctgatgga tttgacactg tatttatgca taacagacga
    11041 cgctgggcgg ctcatcatca agcgtgaagg atttcaacag ttgcagaggt ttgagctttc
    11101 ccgtattcgt atgggggatt taatgtttga accgggggct atgccgaggc tcaaggagct
    11161 tatactctac gacttcatcg gaaagcccaa atctggtgcg gtcgatttcg actttggcat
    11221 ccaacacctc tccagccttg ctcgcctcac tgtcggctta tcctgcgttg gctcgacggt
    11281 ggcggaagta gaggccgcgg aggatgcttt caagagcatg gccgaggcga accccaaccg
    11341 tccaatactg gagatgacaa gatataatac gcagcatatg ttacaagacg agcaaatagg
    11401 tatgacaggc tcaggtacta ttaactagtt tgtacttttg tcttggtgag cggtagatca
    11461 aaagatgtgc atatgcttca atcttttttc cggtaatcta tttcagctac aacgccggcc
    11521 gtcaagtcct aggcttctga cgttgcttca ggtgtgctct caatctatac atatttactc
    11581 atcatattat tctccacctt tttttccatc tctagagctc agcttgtcat tgcattgttc
    11641 aattgtgctt ctctagcagc tacggaaatt ctgaacaggc aaaggtaatt ttacagaagt
    11701 ttctttggca taagtatctg agaaaaatgc acttggcaca atttaccttt tcgcagtaga
    11761 aaagagtcag tggatcatac cttcttaatt gttcgattgc tcgtaatact tgggggcact
    11821 ctatgattgc tgtgtgcttt tagttttgat atagttcctg ttagcatgga cagactgtgt
    11881 gattggttca tgaatttcag ggtggttcta atgaggaaat gatgcttgct tcagaaatag
    11941 tttccatttg tacctaagct gtgtagttgg taaagttgca ttttggatgt tctgttggta
    12001 tagcttacag aagaaagact tgcgttgtgc aggtaccagg ggcgcaggtt actgctagtg
    12061 gcgtctgaga tgtttcataa acgttgggtg ggcaccggtc atgcaaaaaa ttgaaggata
    12121 aaaaggttcc gagaggttgt gtgatgtgct ccttttaaat ggcagtcgct tgcataaaga
    12181 gatgttgctt ggttttttag taaagcgttg tagtaagttg ctctgtttcg gctgagagcc
    12241 atcgttgttg ctgtctgtta catcgaatgg ctggctcatg tcatgttgat gtttttttta
    12301 tctgaaccgg acaaacctga gggtgtagta tcatgtttgt aatgactgaa attctgggct
    12361 cacccgagct ccttaattcg taaacaactg cgactcattg ttggattcct tacttcagaa
    12421 aatatttact tgctctggct catacagctc tgcatgtttt gtagtggttt gtcgctgttt
    12481 aggtgctgct tcagactcct ggtacctttg gccgctgctg ctactgcatg agctttgtat
    12541 ggcattgtta gtatagtttg gtgtcattgg gttttcgccc catggtgagt gtccattgcg
    12601 cgcactcgca gcggtttcct ggcgataagt tgaacttgat ctttctcttt cccacctcct
    12661 aaacgtctat tcagacttgt gtatatttgc attacgggga ttacccgagt tcaaaaataa
    12721 atattattta ctagttctgc caatgtgttt ccgttacata caaataaaag ggtgcttcca
    12781 gctacaagta tatttttgag aagggccatt gcaaaacgaa atgaagccca aattctacca
    12841 acatgtttct gtcacatata cattaaaaaa acggtgtaaa tgtcccgtca aaaaagggtg
    12901 tatgtaggaa aaggagttcg acgccgccag ctccctccta acgctcagat tgtcttcgcg
    12961 gccgccaccg cggctgagtg gctccttcaa gcttgcggat ccgggcggaa ataggtaaag
    13021 aagttgcgga taaggtaatt gccattgcag attatttgga ttgagagtga atatgagact
    13081 ctaattggat accgagggga atttatggaa cgtcagtgga gcatttttga caagaaatat
    13141 ttgctagctg atagtgacct taggcgactt ttgaacgcgc aataatggtt tctgacgtat
    13201 gtgcttagct cattaaactc cagaaacccg cggctgagtg gctccttcaa cgttgcggtt
    13261 ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac
    13321 tcccttaatt ctccgctcat gatcagattg tcgtttcccg ccttcagttt aaactatcag
    13381 tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc
    13441 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac
    13501 cacagggttc ccctcgggag tgcttggcat tccgtgcgat aatgacttct gttcaaccac
    13561 ccaaacgtcg gaaagcctga cgacggagca gcattccaaa aagatccctt ggctcgtctg
    13621 ggtcggctag aaggtcgagt gggctgctgt ggcttgatcc ctcaacgcgg tcgcggacgt
    13681 agcgcagcgc cgaaaaatcc tcgatcgcaa atccgacgct gtcgaaaagc gtgatctgct
    13741 tgtcgctctt tcggccgacg tcctggccag tcatcacgcg ccaaagttcc gtcacaggat
    13801 gatctggcgc gagttgctgg atctcgcctt caatccgggt ctgtggcggg aactccacga
    13861 aaatatccga acgcagcaag atcgtcgacc ctttccgacg ctcaccgggc tggttgccct
    13921 cgccgctggg ctggcggccg tctatggccc tgcaaacgcg ccagaaacgc cgtcgaagcc
    13981 gtgtgcgaga caccgcggcc ggccgccggc gttgtggata cctcgcggaa aacttggccc
    14041 tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc ggcgcggcgt
    14101 tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca gcctcgcaaa
    14161 tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca agcctgggga
    14221 taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga ggggcgcgat
    14281 ccttgacact tgaggggcag agtgctgaca gatgaggggc gcacctattg acatttgagg
    14341 ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt ttcggccacc
    14401 gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt tttaaccagg
    14461 gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct tctcgaaccc
    14521 tcccggcccg ctaacgcggg cctcccatcc ccccaggggc tgcgcccctc ggccgcgaac
    14581 ggcctcaccc caaaaatggc agccaagctc ctaacatttt attagagagc aggctagttg
    14641 cttagataca tgatcttcag gccgttatct gtcagggcaa gcgaaaattg gccatttatg
    14701 acgaccaatg ccccgcagaa gctcccatct ttgccgccat agacgccgcg cccccctttt
    14761 ggggtgtaga acatcctttt gccagatgtg gaaaagaagt tcgttgtccc attgttggca
    14821 atgacgtagt agccggcgaa agtgcgagac ccatttgcgc tatatataag cctacgattt
    14881 ccgttgcgac tattgtcgta attggatgaa ctattatcgt agttgctctc agagttgtcg
    14941 taatttgatg gactattgtc gtaattgctt atggagttgt cgtagttgct tggagaaatg
    15001 tcgtagttgg atggggagta gtcataggga agacgagctt catccactaa aacaattggc
    15061 aggtcagcaa gtgcctgccc cgatgccatc gcaagtacga ggcttagaac caccttcaac
    15121 agatcgcgca tagtcttccc cagctctcta acgcttgagt taagccgcgc cgcgaagcgg
    15181 cgtcggcttg aacgaattgt tagacattat ttgccgacta ccttggtgat ctcgcctttc
    15241 acgtagtgaa caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttgtcca
    15301 agataagcct gcctagcttc aagtatgacg ggctgatact gggccggcag gcgctccatt
    15361 gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg
    15421 cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg cgagttccat
    15481 agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt
    15541 tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata
    15601 gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc attgcgctgc
    15661 cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc
    15721 acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga agccgaagtt
    15781 tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta
    15841 accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa
    15901 tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt
    15961 tgagtcgata cttcggcgat caccgcttcc ctcatgatgt ttaactcctg aattaagccg
    16021 cgccgcgaag cggtgtcggc ttgaatgaat tgttaggcgt catcctgtgc tcccgagaac
    16081 cagtaccagt acatcgctgt ttcgttcgag acttgaggtc tagttttata cgtgaacagg
    16141 tcaatgccgc cgagagtaaa gccacatttt gcgtacaaat tgcaggcagg tacattgttc
    16201 gtttgtgtct ctaatcgtat gccaaggagc tgtctgctta gtgcccactt tttcgcaaat
    16261 tcgatgagac tgtgcgcgac tcctttgcct cggtgcgtgt gcgacacaac aatgtgttcg
    16321 atagaggcta gatcgttcca tgttgagttg agttcaatct tcccgacaag ctcttggtcg
    16381 atgaatgcgc catagcaagc agagtcttca tcagagtcat catccgagat gtaatccttc
    16441 cggtaggggc tcacacttct ggtagatagt tcaaagcctt ggtcggatag gtgcacatcg
    16501 aacacttcac gaacaatgaa atggttctca gcatccaatg tttccgccac ctgctcaggg
    16561 atcaccgaaa tcttcatatg acgcctaacg cctggcacag cggatcgcaa acctggcgcg
    16621 gcttttggca caaaaggcgt gacaggtttg cgaatccgtt gctgccactt gtttaataga
    16681 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
    16741 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
    16801 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    16861 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    16921 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    16981 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    17041 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgatatcc
    17101 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    17161 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    17221 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    17281 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    17341 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    17401 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    17461 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    17521 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    17581 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    17641 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    17701 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    17761 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
    17821 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt
    17881 tctccttacg catctgtgcg gtatttcaca ccgcatatga agatcggcgg caatagcttc
    17941 ttagcgccat cccggctgag aaagcccagt aaggaaacaa ctgtaggttc gagtcgcgag
    18001 atcccccgga accaaaggaa gtaggttaaa cccgctccga tcaggccgag ccacgccagg
    18061 ccgagaacat tggttcctgt aggcatcggg attggcggat caaacactaa agctactgga
    18121 acgagcagaa gtcctccggc cgccagttgc caggcggtaa aggtgagcag aggcacggga
    18181 ggttgccact tgcgggtcag cacggttccg aacgccatgg aaaccgcccc cgccaggccc
    18241 gctgcgacgc cgacaggatc tagcgctgcg tttggtgtca acaccaacag cgccacgccc
    18301 gcagttccgc aaatagcccc caggaccgcc atcaatcgta tcgggctacc tagcagagcg
    18361 gcagagatga acacgaccat cagcggctgc acagcgccta ccgtcgccgc gaccccgccc
    18421 ggcaggcggt agaccgaaat aaacaacaag ctccagaata gcgaaatatt aagtgcgccg
    18481 aggatgaaga tgcgcatcca ccagattccc gttggaatct gtcggacgat catcacgagc
    18541 aataaacccg ccggcaacgc ccgcagcagc ataccggcga cccctcggcc tcgctgttcg
    18601 ggctccacga aaacgccgga cagatgcgcc ttgtgagcgt ccttggggcc gtcctcctgt
    18661 ttgaagaccg acagcccaat gatctcgccg tcgatgtagg cgccgaatgc cacggcatct
    18721 cgcaaccgtt cagcgaacgc ctccatgggc tttttctcct cgtgctcgta aacggacccg
    18781 aacatctctg gagctttctt cagggccgac aatcggatct cgcggaaatc ctgcacgtcg
    18841 gccgctccaa gccgtcgaat ctgagcctta atcacaattg tcaattttaa tcctctgttt
    18901 atcggcagtt cgtagagcgc gccgtgcgtc ccgagcgata ctgagcgaag caagtgcgtc
    18961 gagcagtgcc cgcttgttcc tgaaatgcca gtaaagcgct ggctgctgaa cccccagccg
    19021 gaactgaccc cacaaggccc tagcgtttgc aatgcaccag gtcatcattg acccaggcgt
    19081 gttccaccag gccgctgcct cgcaactctt cgcaggcttc gccgacctgc tcgcgccact
    19141 tcttcacgcg ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt
    19201 acggctcccg gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc gacagcttgc
    19261 ggtacttctc ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct
    19321 cgtcgatcag gacctggcaa cgggacgttt tcttgccacg gtccaggacg cggaagcggt
    19381 gcagcagcga caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg
    19441 cctgtaggcg cgacaggcat tcctcggcct tcgtgtaata ccggccattg atcgaccagc
    19501 ccaggtcctg gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct
    19561 tcgcgtactc caacacctgc tgccacacca gttcgtcatc gtcggcccgc agctcgacgc
    19621 cggtgtaggt gatcttcacg tccttgttga cgtggaaaat gaccttgttt tgcagcgcct
    19681 cgcgcgggat tttcttgttg cgcgtggtga acagggcaga gcgggccgtg tcgtttggca
    19741 tcgctcgcat cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc atttccttga
    19801 tctgctgctt cgtgtgtttc agcaacgcgg cctgcttggc ctcgctgacc tgttttgcca
    19861 ggtcctcgcc ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca
    19921 tcgacttcgc caaacctgcc gcctcctgtt cgagacgacg cgaacgctcc acggcggccg
    19981 atggcgcggg cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag
    20041 cttgctggac catcgagccg acggactgga aggtttcgcg gggcgcacgc atgacggtgc
    20101 ggcttgcgat ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt
    20161 atgccttccg gtcaaacgtc cgattcattc accctccttg cgggattgcc ccgactcacg
    20221 ccggggcaat gtgcccttat tcctgatttg acccgcctgg tgccttggtg tccagataat
    20281 ccaccttatc ggcaatgaag tcggtcccgt agaccgtctg gccgtccttc tcgtacttgg
    20341 tattccgaat cttgccctgc acgaatacca gctccgcgaa gtcgctcttc ttgatggagc
    20401 gcatggggac gtgcttggca atcacgcgca ccccccggcc gttttagcgg ctaaaaaagt
    20461 catggctctg ccctcgggcg gaccacgccc atcatgacct tgccaagctc gtcctgcttc
    20521 tcttcgatct tcgccagcag ggcgaggatc gtggcatcac cgaaccgcgc cgtgcgcggg
    20581 tcgtcggtga gccagagttt cagcaggccg cccaggcggc ccaggtcgcc attgatgcgg
    20641 gccagctcgc ggacgtgctc atagtccacg acgcccgtga ttttgtagcc ctggccgacg
    20701 gccagcaggt aggccgacag gctcatgccg gccgccgccg ccttttcctc aatcgctctt
    20761 cgttcgtctg gaaggcagta caccttgata ggtgggctgc ccttcctggt tgggtaatga
    20821 ctccaactta ttgatagtgt tttatgttca gataatgccc gatgactttg tcatgcagct
    20881 ccaccgattt tgagaacgac agcgacttcc gtcccagccg tgccaggtgc tgcctcagat
    20941 tcaggttatg ccgctcaatt cgctgcgtat atcgcttgct gattacgtgc agctttccct
    21001 tcaggcggga ttcatacagc ggccagccat ccgtcatcca tatcaccacg tcaaagggtg
    21061 acagcaggct cataagacgc cccagcgtcg ccatagtgcg ttcaccgaat acgtgcgcaa
    21121 caaccgtctt ccggagactg tcatacgcgt aaaacagcca gcgctggcgc gatttagccc
    21181 cgacatagcc ccactgttcg tccatttccg cgcagacgat gacgtcactg cccggctgta
    21241 tgcgcgaggt taccgactgc ggcctgagtt ttttaagtga cgtaaaatcg tgttgaggcc
    21301 aacgcccata atgcgggctg ttgcccggca tccaacgcca ttcatggcca tatcaatgat
    21361 tttctggtgc gtaccgggtt gagaagcggt gtaagtgaac tgcagttgcc atgttttacg
    21421 gcagtgagag cagagatagc gctgatgtcc ggcggtgctt ttgccgttac gcaccacccc
    21481 gtcagtagct gaacaggagg gacagctgat agaaacagaa gccactggag cacctcaaaa
    21541 acaccatcat acactaaatc agtaagttgg cagcatcacc cctggttggc ttggtttcat
    21601 cagccatccg cttgccctca tctgttacgc cggcggtagc cggccagcct cgcagagcag
    21661 gattcccgtt gagcaccgcc aggtgcgaat aagggacagt gaagaaggaa cacccgctcg
    21721 cgggtgggcc tacttcacct atcctgcccg gctgacgccg ttggatacac caaggaaagt
    21781 ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa
    21841 aaatcgctat aatgaccccg aagcagggtt atgcagcgga aaagatcc
//
