LOCUS       sequence-200229-       21633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector containing Sr22 PI573523 allele driven by maize 
            ubiquitin promoter, Sr22 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21633)
  AUTHORS   Md Hatta MA, Ghosh S, Athiyannan N, Richardson T, Steuernagel B, Yu 
            G, Rouse MN, Ayliffe M, Lagudah ES, Radhakrishnan GV, Periyannan SK,
            Wulff BBH
  TITLE     Extensive Genetic Variation at the Sr22 Wheat Stem Rust Resistance 
            Gene Locus in the Grasses Revealed Through Evolutionary Genomics and
            Functional Analyses.
  JOURNAL   Mol Plant Microbe Interact. 2020 Nov;33(11):1286-1298. doi: 
            10.1094/MPMI-01-20-0018-R. Epub 2020 Oct 1.
  PUBMED    32779520
REFERENCE   2  (bases 1 to 21633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-01-20-0018-R"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-11"; volume: "33"; issue: "11"; pages: 
            "1286-1298"
COMMENT     Sequence Label: pBW_0005
FEATURES             Location/Qualifiers
     source          1..21633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    416..440
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        1482..1827
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1770..1790
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    1857..1912
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             1914..2465
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      2488..3195
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(12791..12974)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(12827..13006)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    13130..13154
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      13642..14277
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(14952..15740)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      16837..17425
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     17326..17345
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     17579..17596
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(18785..19933)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     mobile_element  20558..21325
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     oriT            21385..21494
                     /note="incP origin of transfer"
ORIGIN
        1 gtcgacgatc gtcaacgttc acttctaaag aaatagcgcc actcagcttc ctcagcggct
       61 ttatccagcg atttcctatt atgtcggcat agttctcaag atcgacagcc tgtcacggtt
      121 aagcgagaaa tgaataagaa ggctgataat tcggatctct gcgagggaga tgatatttga
      181 tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc
      241 cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca
      301 aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg
      361 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca
      421 ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt
      481 ttttaatgta ctgaattcca atcccacaaa aatctgagct taacagcaca gttgctcctc
      541 tcagagcaga atcgggtatt caacaccctc atatcaacta ctacgttgtg tataacggtc
      601 cacatgccgg tatatacgat gactggggtt gtacaaaggc ggcaacaaac ggcgttcccg
      661 gagttgcaca caagaaattt gccactatta cagaggcaag agcagcagct gacgcgtaca
      721 caacaagtca gcaaacagac aggttgaact tcatccccaa aggagaagct caactcaagc
      781 ccaagagctt tgctaaggcc ctaacaagcc caccaaagca aaaagcccac tggctcacgc
      841 taggaaccaa aaggcccagc agtgatccag ccccaaaaga gatctccttt gccccggaga
      901 ttacaatgga cgatttcctc tatctttacg atctaggaag gaagttcgaa ggtgaaggtg
      961 acgacactat gttcaccact gataatgaga aggttagcct cttcaatttc agaaagaatg
     1021 ctgacccaca gatggttaga gaggcctacg cagcaggtct catcaagacg atctacccga
     1081 gtaacaatct ccaggagatc aaataccttc ccaagaaggt taaagatgca gtcaaaagat
     1141 tcaggactaa ttgcatcaag aacacagaga aagacatatt tctcaagatc agaagtacta
     1201 ttccagtatg gacgattcaa ggcttgcttc ataaaccaag gcaagtaata gagattggag
     1261 tctctaaaaa ggtagttcct actgaatcta aggccatgca tggagtctaa gattcaaatc
     1321 gaggatctaa cagaactcgc cgtgaagact ggcgaacagt tcatacagag tcttttacga
     1381 ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacactct ggtctactcc
     1441 aaaaatgtca aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg
     1501 ataatttcgg gaaacctcct cggattccat tgcccagcta tctgtcactt catcgaaagg
     1561 acagtagaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggctatc
     1621 attcaagatc tctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc
     1681 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgacatctcc
     1741 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa
     1801 ggaagttcat ttcatttgga gaggacacgc tcgagtataa gagctctatt tttacaacaa
     1861 ttaccaacaa caacaaacaa caaacaacat tacaattact atttacaatt accatgagcc
     1921 cagaacgacg cccggccgac atccgccgtg ccaccgaggc ggacatgccg gcggtctgca
     1981 ccatcgtcaa ccactacatc gagacaagca cggtcaactt ccgtaccgag ccgcaggaac
     2041 cgcaggagtg gacggacgac ctcgtccgtc tgcgggagcg ctatccctgg ctcgtcgccg
     2101 aggtggacgg cgaggtcgcc ggcatcgcct acgcgggccc ctggaaggca cgcaacgcct
     2161 acgactggac ggccgagtca accgtgtacg tctccccccg ccaccagcgg acgggactgg
     2221 gctccacgct ctacacccac ctgctgaagt ccctggaggc acagggcttc aagagcgtgg
     2281 tcgctgtcat cgggctgccc aacgacccga gcgtgcgcat gcacgaggcg ctcggatatg
     2341 ccccccgcgg catgctgcgg gcggccggct tcaagcacgg gaactggcat gacgtgggtt
     2401 tctggcagct ggacttcagc ctgccggtac cgccccgtcc ggtcctgccc gtcaccgaga
     2461 tttgatgacc cgggggatcc tagagtcctg ctttaatgag atatgcgaga cgcctatgat
     2521 cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc
     2581 tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt tactatcgta
     2641 tttttatgaa taatattctc cgttcaattt actgattgta ccctactact tatatgtaca
     2701 atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc tatgatagag
     2761 cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa aaaagcggca
     2821 gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca aaagtgcccc
     2881 aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg aagggaactc
     2941 cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg ccgtccgctc
     3001 taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt aaccgacttg
     3061 ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg aagtgcaggt
     3121 caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt gcgacaacat
     3181 gtcgaggctc agcaggacct gcaggcatgc aagctctaga gcggccgccg ccgcgacgac
     3241 tcgacgatta gtcggcgatt agtcggcgac tcaaaaacct tgtaaacagt caacatcgtg
     3301 gtaatatacg caccacaaac ttgaaaactt gagcaaaaac accatgataa ctttgacctg
     3361 gatggaggaa gttgttgaaa agcataccct cggtaattta tgtgcaaata acatgataat
     3421 ttacccatcg gagagctaat aactctcgtt caaacaccac aataattttt tgaccctgaa
     3481 ttttttttcg ttgaagaaca ttacccccaa taacttctag gtaaatagca cggtaatata
     3541 tttattgcag atacaataac ttacgcaaaa acagtatgct agctttgacc cacgtaaaaa
     3601 attgtttgaa gcatatcgtt ggtaaatttt gtttaaatag aatggtaata tacgcattgc
     3661 agacatgata agttactcac aaacatcatg ataagtttga ttcgggtgga tgaagttgtt
     3721 gaaaatatac ccggtaactt gtgtgtaaac agcacggtaa tagtcttgat ttggggaaga
     3781 atagtaaaaa attgtcaaaa aatacactgg taacttttgt atgtaaatag catagtaaat
     3841 taggcaccac aaacttgata atttagatac aaacaccgcg gtaaattaga gattttttta
     3901 aaagatagcc atggtaatat acccatcgga tacctgataa cttacgtaca tatactatgg
     3961 taatatttga ctcggtgggg aaattattga aaacagacac cccccccccc cctcccggta
     4021 acttatatgt aaacatcatg gtaatatatg aactgtagtg tcggtgaacc atttttatgc
     4081 ctccgatgac ttatgtggaa atagcacggt aacttgcata taaaaggcat ggtaaatttt
     4141 ggttaaaaaa agtcatcgaa acatgtcaac ataggatcta gtttcaaaga tctcatcgcg
     4201 acgaatcgta tatgtaaaaa cggtttttac atcggaccga cggtttgagc tacaaaacat
     4261 tttaaatatt ttaataaaga aaatctagaa tgacatcgtc ttttttgtcc tctaatgtat
     4321 gcatctaatt agaaggagaa atgcacgtga aagaagaaag aatcaaacta atgtatacat
     4381 ctatttagag tgaaggaaat agaggtgtga cagttttgtt tatatggcac acatgtgcca
     4441 aatatcatag tccgatagaa tatagatgca cgctagatct ttttcttctg ataagctaca
     4501 cggtatgtgt ttcctaacaa cagaaatctg ggttacatat caaagtctca atctaagaat
     4561 acacaaatat taatggaaaa aaatactcaa atgacaatct acttatgccc aaaagaaaag
     4621 aaaaaaattg catccgactc tgaattcgtg atacttgtct tacttattcc tagtcattcc
     4681 actaattcgt gatacttgca gacatgtaca gaagtatcat ttgctgcagc atccaatccg
     4741 agtacgtgac ttgggctcca taaccaaagg cgcatctcgg tttaggcaaa gcctaggact
     4801 tgacggccgg cgttgtagct gaaatagatt accggaaaaa agattgaagc atatgcacat
     4861 cttttgatct accgctcacc aagacaaaag tacaaactag ttaatagtac ctgagcctgt
     4921 catacctatt tgctcgtctt gtaacatatg ctgcgtatta tatcttgtca tctccagtat
     4981 tggacggttg gggttcgcct cggccatgct cttgaaagca tcctccgcgg cctctacttc
     5041 cgccaccgtc gagccaacgc aggataagcc gacagtgagg cgagcaaggc tggagaggtg
     5101 ttggatgcca aagtcgaaat cgaccgcacc agatttgggc tttccgatga agtcgtagag
     5161 tataagctcc ttgagcctcg gcatagcccc cggttcaaac attaaatccc ccatacgaat
     5221 acgggaaagc tcaaacctct gcaactgttg aaatccttca cgcttgatga tgagccgccc
     5281 agcgtcgtct gttatgcata aatacagtgt caaatccatc agattgggta tcaatccaat
     5341 catctcaata tcctgctgct ctatcttcgg ttggttcaaa aacaacctgt gtaggttggg
     5401 aagtgacgcg agccatctgc taatccggct gatatcttgt ccatgtacaa aaacctcctg
     5461 gatacaaccc aaagcaggga ggaacggctc ttctccatcc ttttctctca gataataata
     5521 aatactcagg gaaaggaggc cgcattcgtc cagtttacag agagaggaca ccagcttttc
     5581 cttggactgt tctaatttgt tgccttcaat cccatcagtg tccaatttaa tcctcagctt
     5641 tctcagattt gttagtttgc cgagctcttc cacatgcttc aactgcaagg catcaacgca
     5701 gtcaagctct tgcaagttta tcaagtttcc caacccatct ggaagcttac aaccgctgtc
     5761 aacaaacaaa cgtgccagtc gttgtagccg acttgtgctt tcaggaagct cacgtaatcc
     5821 ataggatgta ctaactagcg tctctagata cagcaactcc ccaatttgcc tgggaagctc
     5881 cgtgattcct gtaaaagaga tatccaagta tctcagctga tagagtcttt caatatcctt
     5941 gatatgatga tttttcaact gatagcagcc ttgcaagtct agcacgcgca agcattttga
     6001 cctcccaagc gaaggcaata gcttgatatc cccaaaagca ctgattgacc gagcgtgtga
     6061 cagatccaat tgctccatga tttcttcatc ctcattcccc attagagaga gccgacgaac
     6121 tatgccatta ggcttgcatt gtttcttcag gacagtacac atgttctctt cagaggattt
     6181 gtcaattagg aaatcaagga tggtgtcatg gactcggcaa tactccgcct tgccgtcata
     6241 ccctatgttg ccgggctgta ttagacttct gttcacaagc tggtgaaaat attcctctcc
     6301 taattccatg agatcttgtc cactttctcc atggataaag ccttcagaaa tccatctgcg
     6361 tactaaccgc ttccttccaa tcaattggtc ttcaggaaac agagccaggt acaataagca
     6421 gcttcttagg tggtggggaa gatcaaaata actaagagac aatatgtaat tcatggtttc
     6481 tattatatca cttttgacct ttgaagagcg tattgaatgt cgcacccgat cccactcttc
     6541 ttttgtttcc cttgttgtga ccaacaagct agaaatggca ttgatggcca gaggtaggcc
     6601 acggcatttt ttcaaaatat cattggcagc ttctttcaag ctatcaggac agctttcttc
     6661 acagccaaat atcctcttga aaaacaattt cttcgagtca gcctcactga gtggtttcat
     6721 ctcatagaca agatcttcac tagaaggaga acaagatttg gcaacatcaa ctatacgtgt
     6781 tgtgataatt attctgctgc ccaatgtatt cttgacaaat gcacatttca aagtttccca
     6841 tgttcctaac tcccatatat catcaattat gataaagtac ctgcaacaca gtttcatgaa
     6901 catacacatt agagctcatt agatatttat agtaacacct accaagtaac ctaaaatcct
     6961 gaaacaatct cacatctgtg tgcatcctag atataataca ccttgatgta attgtctagc
     7021 atcaagaaag ttgtcattat ctaaaataag tgaaatcctg agagcatctc acaataatac
     7081 acagctacag atgggggaga aactcaaaga taacaactcc acacatagta gtactaggta
     7141 caagcaactc taacacgcca acggatttgg tccgtgtgtg tccattaggg tcgaaaggca
     7201 caaaaacgcc ggcccaacgt gcggacacaa acccaaatca cgtccgttcg gcatccatgt
     7261 agacgcattt ccagcccaaa tttgtgcctg atttgcgttc acacgaacac gagacggacg
     7321 tgttgcgcgt ccgctcggtg tgcgcctcgg gctggcgtgt ctgccagcca actaccacca
     7381 cagacgtagt caatgccgcc cacctacctc gggcccgcat ggcagtgact agaaacgtcc
     7441 ggatcacgcc ctttttaatt gcaagggtgc ggcggggcgg gcctcatcca cttctcctcc
     7501 cactctagtc cacatctgct ccctcgccgg tgacagaaaa ccctagccgt catgggtttc
     7561 tttggcaaag gtaagggcaa ggcaagcacg accacgaggc cggctcgttg caagggccgg
     7621 tgaggccggc agcgaagggg ttcgcattgg tgtcggcgct gcgggatggg agggcggcag
     7681 cgccacggga ccaccagtac atcccggtgc accacgcgta gttagtacca ccgggagtac
     7741 cgcgtctcac tgccatggcc ggacgtgaac ttcccccacg gctggcacct cgacccccac
     7801 cacattccag tgccggcggt gccacagtcc gagcgtgcgc gcgccaacga gattcgggcc
     7861 cggcgtgctc tcctgacgcc agagcagcgt cgcgactcca cccatcgagg ccagactccc
     7921 ctaactgaga agtctggttc accatcgagc accatgtgcg cctccgcgac gccgtccagc
     7981 tcgaagcctg ccctccaccg ccgccaccgg tggtcaaata gtaggttgag gaggaggcgg
     8041 cgttcctcaa ggcattggag gagggggccc atcacgtcac cgaggagtct tagtgcgagg
     8101 actcctagtg gtctggcctc gatgacacgc tgcagctatc ggccgccaac tatagtgtct
     8161 acctgccggg accaccagcc acatcgtcac cgcctgcacc agcgctagca ccacccgcgc
     8221 ccaccggcgt ttaccaatgg cagggcatcg tctgcaagcg gatgagcaag ccgcccattt
     8281 ggctgtgcgt gatggaggag gaggcggcct acctcgtggc gtggaaggac cgcacgctgg
     8341 aggaggagcg gtacgacggc caggtccgca tgcagcggga acccgagggg gaggagcgcc
     8401 aaaacttcgt tgggcgtgcg atgaggagga ggagcggcag gagcgcgagg aggaggcgtg
     8461 cgcggtgcca ggtagggccg ccgccgccgc agacagcgga gcaggcggtg ctggctgccc
     8521 accagcaggc cttcccgtgg gctgagctgc cggcattctt cgacctcacc cactcctccg
     8581 acgaagacgc ctagggctgc gggctggggc ggctgttttt tattaatata ttaatgtatt
     8641 atgtttaatt atgtggactt tgatgggctt taatatgcat ttttatttat atttaattat
     8701 tttaagcttt ttaagttttt taaatgccca cgcggactaa tgcgtaggcg caccacccaa
     8761 aggcaaaacc agacaccccg tccgcttggc cgatctaaat ggacaaaaat tgcgtcggcg
     8821 tgttggagtt gctcttagat acaatttggt ggcaatataa gaaaaaaaaa gattgccata
     8881 gaaggcaagc gaaaaatagc aagatttaac aaccaagagg aaaaaaatat gaatgctcag
     8941 gccgagtaaa ggcagctgtt gccagtggca aggtacaatg ttttgccact gccaatagca
     9001 tgttcgcaag aggatagata ttgaaacaat gatggggact acctattttc ttttgtcatt
     9061 ttggtaacaa gttatttcct catgattggt aggaccacat attgagtggt ggctctgcgc
     9121 ctcaattcgt catgcaaatt gctacgaaac caggaaagac gcaacaacct actgttgtca
     9181 ttatgttacg ctagtcaggc tggtccgatc tgactatgat taattcttcc cttttctctt
     9241 tttgtgttat gggcatagat tcttcaacgg atgtttgata tccattcaag attataatac
     9301 tgataaccgt aggtttatag cctctagaaa ccctatgttc ttaaagtggc taatcacaaa
     9361 cctaatgtac tgcatatccg gtaacaatgg tctgcacatg caagtgcttt gggtccttag
     9421 aagatagata tatttgcagt catgtagtgc gtgcggatat ttatcttgta caaaagagag
     9481 ggaattgcat gtactttatt gttttgactg acgccatgtc acaattattt aataaacaga
     9541 acatagccgc aagtttaaca aacttgtggc aatcaaatta gaaaatgtgt ggcgacagaa
     9601 atcggcaaac acattttagc tatatcttaa ttagtgaaca gatcgacata agttgcaaga
     9661 aacaatgact tgaaagttta agcgcagtca tacaagttga ggacgaaagt aaatcacaca
     9721 aagcaatctt cattagcaaa attctaggca accgattatg catgagctaa ttaagcaatg
     9781 ttacttcgta gcagatctca catacatacc ttgtgtcttg gaggaattct cgaatcttgt
     9841 cgatgatgag ttgtatatca tctaatctag agcaagcatc gtctttcttg gtgatttgtg
     9901 ataatataga tcttaatatc gccttcatgt caggcttgcg tgaaatcgag acaaaagccc
     9961 cgcagtcgaa ggtcgctcca aggttgaccc ggatctggtt ggcgagcgtg gtcttgccca
    10021 accctccata tccaacaata gagacagatt tcagtgattg gccctctttc tcattcagcc
    10081 acttgacaag ttcatccttt ggtccatcaa ttccgacgag ctctgcggcg tctttgtaga
    10141 ctgcacgaag tcgagggtcg accttgcctt ttgccggcat agaggaggag gactcgtcaa
    10201 tcttgtacct tgagtggcaa caaatcggtc acaaatctga tggtaggtac taatagtact
    10261 gtaaagagaa caagatctgg taccttgaaa atctgtcgct gacatcctta atttctttct
    10321 tgatgtcttt gacctccttg gcgatcctgc ggcgggtctt gatgtccggc agcaagttct
    10381 tgcacttgtc catgagcttg gcgacgccat gagccgcctc ggagcaggag gactcgtgtt
    10441 ccacgaggac catgaacttg tcgatgttgt cctctatctt ataggacatc tcccgcaccg
    10501 ccgtcacacg cagctttgtt ggttcgtcag gctcctccac atctgacatc ttgaggagga
    10561 acgcgcacat gacctccagc tcgtccttga ggaacttgat gtccccacgg acgttcttga
    10621 gcagcttgta ttcatccgtc aacatagctc ccaacttccg gaggagggaa cccattgcgc
    10681 ctgtggatgc gctcaccaga acttccgcca tcattcctgc agaagtaaca ccaaacaaca
    10741 gggtgagcat cgacaaaaga aacagtacca agcaaataaa tagcgtatga aggcagggct
    10801 aaaaaaatcc acatatagct gctgcatatg ccatcatcca agtatatcaa gatcaaaata
    10861 attataaaac atacttgttt attataatag ataggtactc aaggttagag catatgaata
    10921 gatgctgcat atgccatcat gtatatgcat cagtaaaacc cacatcaaca tgtataccta
    10981 tcctagatcg atatttccat ccatcttaaa ctcgtaacta tgaagatgta tgacacacac
    11041 atacagttcc aaaattaata aatacaccag gtagtttgaa acagtattct actccgatct
    11101 agaacgaatg aacgaccgcc caaccacacc acatcatcac aaccaagcga acaaaaagca
    11161 tctctgtata tgcatcagta aaacccgcat caacatgtat acctatccta gatcgatatt
    11221 tccatccatc atcttcaatt cgtaactatg aatatgtatg gcacacacat acagatccaa
    11281 aattaataaa tccaccaggt agtttgaaac agaattctac tccgatctag aacgaccgcc
    11341 caaccagacc acatcatcac aaccaagaca aaaaaaagca tgaaaagatg acccgacaaa
    11401 caagtgcacg gcatatattg aaataaagga aaagggcaaa ccaaacccta tgcaacgaaa
    11461 caaaaaaaat catgaaatcg atcccgtctg cggaacggct agagccatcc caggattccc
    11521 caaagagaaa cactggcaag ttagcaatca gaacgtgtct gacgtacagg tcgcatccgt
    11581 gtacgaacgc tagcagcacg gatctaacac aaacacggat ctaacacaaa catgaacaga
    11641 agtagaacta ccgggcccta accatggacc ggaacgccga tctagagaag gtagagaggg
    11701 ggggggggga ggacgagcgg cgtaccttga agcggaggtg ccgacgggtg gatttggggg
    11761 agatctggtt gtgtgtgtgt gcgctccgaa caacacgagg ttggggaaag agggtgtgga
    11821 gggggtgtct atttattacg gcgggcgagg aagggaaagc gaaggagcgg tgggaaagga
    11881 atcccccgta gctgccgtgc cgtgagagga ggaggaggcc gcctgccgtg ccggctcacg
    11941 tctgccgctc cgccacgcaa tttctggatg ccgacagcgg agcaagtcca acggtggagc
    12001 ggaactctcg agaggggtcc agaggcagcg acagagatgc cgtgccgtct gcttcgcttg
    12061 gcccgacgcg acgctgctgg ttcgctggtt ggtgtccgtt agactcgtcg acggcgttta
    12121 acaggctggc attatctact cgaaacaaga aaaatgtttc cttagttttt ttaatttctt
    12181 aaagggtatt tgtttaattt ttagtcactt tattttattc tattttatat ctaaattatt
    12241 aaataaaaaa actaaaatag agttttagtt ttcttaattt agaggctaaa atagaataaa
    12301 atagatgtac taaaaaaatt agtctataaa aaccattaac cctaaaccct aaatggatgt
    12361 actaataaaa tggatgaagt attatatagg tgaagctatt tgcaaaaaaa aaggagaaca
    12421 catgcacact aaaaagataa aactgtagag tcctgttgtc aaaatactca attgtccttt
    12481 agaccatgtc taactgttca tttatatgat tctctaaaac actgatatta ttgtagtact
    12541 atagattata ttattcgtag agtaaagttt aaatatatgt ataaagatag ataaactgca
    12601 cttcaaacaa gtgtgacaaa aaaaatatgt ggtaattttt tataacttag acatgcaatg
    12661 ctcattatct ctagagaggg gcacgaccgg gtcacgctgc acgcggccgc caccgcggct
    12721 gagtggctcc ttcaagcttg cggatccggg cggaaatagg taaagaagtt gcggataagg
    12781 taattgccat tgcagattat ttggattgag agtgaatatg agactctaat tggataccga
    12841 ggggaattta tggaacgtca gtggagcatt tttgacaaga aatatttgct agctgatagt
    12901 gaccttaggc gacttttgaa cgcgcaataa tggtttctga cgtatgtgct tagctcatta
    12961 aactccagaa acccgcggct gagtggctcc ttcaacgttg cggttctgtc agttccaaac
    13021 gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac gtgactccct taattctccg
    13081 ctcatgatca gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat
    13141 attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg
    13201 cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc
    13261 gggagtgctt ggcattccgt gcgataatga cttctgttca accacccaaa cgtcggaaag
    13321 cctgacgacg gagcagcatt ccaaaaagat cccttggctc gtctgggtcg gctagaaggt
    13381 cgagtgggct gctgtggctt gatccctcaa cgcggtcgcg gacgtagcgc agcgccgaaa
    13441 aatcctcgat cgcaaatccg acgctgtcga aaagcgtgat ctgcttgtcg ctctttcggc
    13501 cgacgtcctg gccagtcatc acgcgccaaa gttccgtcac aggatgatct ggcgcgagtt
    13561 gctggatctc gccttcaatc cgggtctgtg gcgggaactc cacgaaaata tccgaacgca
    13621 gcaagatcgt cgaccctttc cgacgctcac cgggctggtt gccctcgccg ctgggctggc
    13681 ggccgtctat ggccctgcaa acgcgccaga aacgccgtcg aagccgtgtg cgagacaccg
    13741 cggccggccg ccggcgttgt ggatacctcg cggaaaactt ggccctcact gacagatgag
    13801 gggcggacgt tgacacttga ggggccgact cacccggcgc ggcgttgaca gatgaggggc
    13861 aggctcgatt tcggccggcg acgtggagct ggccagcctc gcaaatcggc gaaaacgcct
    13921 gattttacgc gagtttccca cagatgatgt ggacaagcct ggggataagt gccctgcggt
    13981 attgacactt gaggggcgcg actactgaca gatgaggggc gcgatccttg acacttgagg
    14041 ggcagagtgc tgacagatga ggggcgcacc tattgacatt tgaggggctg tccacaggca
    14101 gaaaatccag catttgcaag ggtttccgcc cgtttttcgg ccaccgctaa cctgtctttt
    14161 aacctgcttt taaaccaata tttataaacc ttgtttttaa ccagggctgc gccctgtgcg
    14221 cgtgaccgcg cacgccgaag gggggtgccc ccccttctcg aaccctcccg gcccgctaac
    14281 gcgggcctcc catcccccca ggggctgcgc ccctcggccg cgaacggcct caccccaaaa
    14341 atggcagcca agctcctaac attttattag agagcaggct agttgcttag atacatgatc
    14401 ttcaggccgt tatctgtcag ggcaagcgaa aattggccat ttatgacgac caatgccccg
    14461 cagaagctcc catctttgcc gccatagacg ccgcgccccc cttttggggt gtagaacatc
    14521 cttttgccag atgtggaaaa gaagttcgtt gtcccattgt tggcaatgac gtagtagccg
    14581 gcgaaagtgc gagacccatt tgcgctatat ataagcctac gatttccgtt gcgactattg
    14641 tcgtaattgg atgaactatt atcgtagttg ctctcagagt tgtcgtaatt tgatggacta
    14701 ttgtcgtaat tgcttatgga gttgtcgtag ttgcttggag aaatgtcgta gttggatggg
    14761 gagtagtcat agggaagacg agcttcatcc actaaaacaa ttggcaggtc agcaagtgcc
    14821 tgccccgatg ccatcgcaag tacgaggctt agaaccacct tcaacagatc gcgcatagtc
    14881 ttccccagct ctctaacgct tgagttaagc cgcgccgcga agcggcgtcg gcttgaacga
    14941 attgttagac attatttgcc gactaccttg gtgatctcgc ctttcacgta gtgaacaaat
    15001 tcttccaact gatctgcgcg cgaggccaag cgatcttctt gtccaagata agcctgccta
    15061 gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg
    15121 acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc
    15181 actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca
    15241 tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga
    15301 cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg
    15361 atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc
    15421 agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact
    15481 tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg
    15541 atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata
    15601 tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac
    15661 gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg
    15721 gcgatcaccg cttccctcat gatgtttaac tcctgaatta agccgcgccg cgaagcggtg
    15781 tcggcttgaa tgaattgtta ggcgtcatcc tgtgctcccg agaaccagta ccagtacatc
    15841 gctgtttcgt tcgagacttg aggtctagtt ttatacgtga acaggtcaat gccgccgaga
    15901 gtaaagccac attttgcgta caaattgcag gcaggtacat tgttcgtttg tgtctctaat
    15961 cgtatgccaa ggagctgtct gcttagtgcc cactttttcg caaattcgat gagactgtgc
    16021 gcgactcctt tgcctcggtg cgtgtgcgac acaacaatgt gttcgataga ggctagatcg
    16081 ttccatgttg agttgagttc aatcttcccg acaagctctt ggtcgatgaa tgcgccatag
    16141 caagcagagt cttcatcaga gtcatcatcc gagatgtaat ccttccggta ggggctcaca
    16201 cttctggtag atagttcaaa gccttggtcg gataggtgca catcgaacac ttcacgaaca
    16261 atgaaatggt tctcagcatc caatgtttcc gccacctgct cagggatcac cgaaatcttc
    16321 atatgacgcc taacgcctgg cacagcggat cgcaaacctg gcgcggcttt tggcacaaaa
    16381 ggcgtgacag gtttgcgaat ccgttgctgc cacttgttta atagactgga tggaggcgga
    16441 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
    16501 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
    16561 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
    16621 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
    16681 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    16741 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    16801 agcgtcagac cccgtagaaa agatcaaagg atcttcttga tatccttttt ttctgcgcgt
    16861 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    16921 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    16981 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    17041 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    17101 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    17161 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    17221 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    17281 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    17341 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    17401 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    17461 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    17521 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
    17581 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
    17641 gtgcggtatt tcacaccgca tatgaagatc ggcggcaata gcttcttagc gccatcccgg
    17701 ctgagaaagc ccagtaagga aacaactgta ggttcgagtc gcgagatccc ccggaaccaa
    17761 aggaagtagg ttaaacccgc tccgatcagg ccgagccacg ccaggccgag aacattggtt
    17821 cctgtaggca tcgggattgg cggatcaaac actaaagcta ctggaacgag cagaagtcct
    17881 ccggccgcca gttgccaggc ggtaaaggtg agcagaggca cgggaggttg ccacttgcgg
    17941 gtcagcacgg ttccgaacgc catggaaacc gcccccgcca ggcccgctgc gacgccgaca
    18001 ggatctagcg ctgcgtttgg tgtcaacacc aacagcgcca cgcccgcagt tccgcaaata
    18061 gcccccagga ccgccatcaa tcgtatcggg ctacctagca gagcggcaga gatgaacacg
    18121 accatcagcg gctgcacagc gcctaccgtc gccgcgaccc cgcccggcag gcggtagacc
    18181 gaaataaaca acaagctcca gaatagcgaa atattaagtg cgccgaggat gaagatgcgc
    18241 atccaccaga ttcccgttgg aatctgtcgg acgatcatca cgagcaataa acccgccggc
    18301 aacgcccgca gcagcatacc ggcgacccct cggcctcgct gttcgggctc cacgaaaacg
    18361 ccggacagat gcgccttgtg agcgtccttg gggccgtcct cctgtttgaa gaccgacagc
    18421 ccaatgatct cgccgtcgat gtaggcgccg aatgccacgg catctcgcaa ccgttcagcg
    18481 aacgcctcca tgggcttttt ctcctcgtgc tcgtaaacgg acccgaacat ctctggagct
    18541 ttcttcaggg ccgacaatcg gatctcgcgg aaatcctgca cgtcggccgc tccaagccgt
    18601 cgaatctgag ccttaatcac aattgtcaat tttaatcctc tgtttatcgg cagttcgtag
    18661 agcgcgccgt gcgtcccgag cgatactgag cgaagcaagt gcgtcgagca gtgcccgctt
    18721 gttcctgaaa tgccagtaaa gcgctggctg ctgaaccccc agccggaact gaccccacaa
    18781 ggccctagcg tttgcaatgc accaggtcat cattgaccca ggcgtgttcc accaggccgc
    18841 tgcctcgcaa ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg
    18901 aatccgatcc gcacatgagg cggaaggttt ccagcttgag cgggtacggc tcccggtgcg
    18961 agctgaaata gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac ttctcccata
    19021 tgaatttcgt gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct
    19081 ggcaacggga cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg
    19141 attccaggtg cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca
    19201 ggcattcctc ggccttcgtg taataccggc cattgatcga ccagcccagg tcctggcaaa
    19261 gctcgtagaa cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg tactccaaca
    19321 cctgctgcca caccagttcg tcatcgtcgg cccgcagctc gacgccggtg taggtgatct
    19381 tcacgtcctt gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc gggattttct
    19441 tgttgcgcgt ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt
    19501 ccggccacgg cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt
    19561 gtttcagcaa cgcggcctgc ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg
    19621 tttttcgctt cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac
    19681 ctgccgcctc ctgttcgaga cgacgcgaac gctccacggc ggccgatggc gcgggcaggg
    19741 cagggggagc cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg
    19801 agccgacgga ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt
    19861 cggcatcctc ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa
    19921 acgtccgatt cattcaccct ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc
    19981 cttattcctg atttgacccg cctggtgcct tggtgtccag ataatccacc ttatcggcaa
    20041 tgaagtcggt cccgtagacc gtctggccgt ccttctcgta cttggtattc cgaatcttgc
    20101 cctgcacgaa taccagctcc gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct
    20161 tggcaatcac gcgcaccccc cggccgtttt agcggctaaa aaagtcatgg ctctgccctc
    20221 gggcggacca cgcccatcat gaccttgcca agctcgtcct gcttctcttc gatcttcgcc
    20281 agcagggcga ggatcgtggc atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag
    20341 agtttcagca ggccgcccag gcggcccagg tcgccattga tgcgggccag ctcgcggacg
    20401 tgctcatagt ccacgacgcc cgtgattttg tagccctggc cgacggccag caggtaggcc
    20461 gacaggctca tgccggccgc cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg
    20521 cagtacacct tgataggtgg gctgcccttc ctggttgggt aatgactcca acttattgat
    20581 agtgttttat gttcagataa tgcccgatga ctttgtcatg cagctccacc gattttgaga
    20641 acgacagcga cttccgtccc agccgtgcca ggtgctgcct cagattcagg ttatgccgct
    20701 caattcgctg cgtatatcgc ttgctgatta cgtgcagctt tcccttcagg cgggattcat
    20761 acagcggcca gccatccgtc atccatatca ccacgtcaaa gggtgacagc aggctcataa
    20821 gacgccccag cgtcgccata gtgcgttcac cgaatacgtg cgcaacaacc gtcttccgga
    20881 gactgtcata cgcgtaaaac agccagcgct ggcgcgattt agccccgaca tagccccact
    20941 gttcgtccat ttccgcgcag acgatgacgt cactgcccgg ctgtatgcgc gaggttaccg
    21001 actgcggcct gagtttttta agtgacgtaa aatcgtgttg aggccaacgc ccataatgcg
    21061 ggctgttgcc cggcatccaa cgccattcat ggccatatca atgattttct ggtgcgtacc
    21121 gggttgagaa gcggtgtaag tgaactgcag ttgccatgtt ttacggcagt gagagcagag
    21181 atagcgctga tgtccggcgg tgcttttgcc gttacgcacc accccgtcag tagctgaaca
    21241 ggagggacag ctgatagaaa cagaagccac tggagcacct caaaaacacc atcatacact
    21301 aaatcagtaa gttggcagca tcacccctgg ttggcttggt ttcatcagcc atccgcttgc
    21361 cctcatctgt tacgccggcg gtagccggcc agcctcgcag agcaggattc ccgttgagca
    21421 ccgccaggtg cgaataaggg acagtgaaga aggaacaccc gctcgcgggt gggcctactt
    21481 cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca cgaacccttt
    21541 ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatc gctataatga
    21601 ccccgaagca gggttatgca gcggaaaaga tcc
//
