LOCUS       sequence-200231-       21313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pVec8 containing Sr22 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21313)
  AUTHORS   Md Hatta MA, Ghosh S, Athiyannan N, Richardson T, Steuernagel B, Yu 
            G, Rouse MN, Ayliffe M, Lagudah ES, Radhakrishnan GV, Periyannan SK,
            Wulff BBH
  TITLE     Extensive Genetic Variation at the Sr22 Wheat Stem Rust Resistance 
            Gene Locus in the Grasses Revealed Through Evolutionary Genomics and
            Functional Analyses.
  JOURNAL   Mol Plant Microbe Interact. 2020 Nov;33(11):1286-1298. doi: 
            10.1094/MPMI-01-20-0018-R. Epub 2020 Oct 1.
  PUBMED    32779520
REFERENCE   2  (bases 1 to 21313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-01-20-0018-R"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-11"; volume: "33"; issue: "11"; pages: 
            "1286-1298"
COMMENT     Sequence Label: pBW_0065
FEATURES             Location/Qualifiers
     source          1..21313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    480..504
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        628..807
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        660..843
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      complement(913..1165)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     intron          1893..2082
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     promoter        complement(2431..2775)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(2467..2487)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    12874..12898
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     oriT            13453..13562
                     /note="incP origin of transfer"
     mobile_element  13622..14389
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             15014..16162
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     primer_bind     complement(17351..17368)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(17522..18110)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(17602..17621)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             19207..19995
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      20670..21305
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcgacgatct tgctgcgttc ggatattttc gtggagttcc cgccacagac ccggattgaa
       61 ggcgagatcc agcaactcgc gccagatcat cctgtgacgg aactttggcg cgtgatgact
      121 ggccaggacg tcggccgaaa gagcgacaag cagatcacgc ttttcgacag cgtcggattt
      181 gcgatcgagg atttttcggc gctgcgctac gtccgcgacc gcgttgaggg atcaagccac
      241 agcagcccac tcgaccttct agccgaccca gacgagccaa gggatctttt tggaatgctg
      301 ctccgtcgtc aggctttccg acgtttgggt ggttgaacag aagtcattat cgcacggaat
      361 gccaagcact cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat
      421 aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg
      481 tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca
      541 atctgatcat gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga
      601 caagccgttt tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctcagccgcg
      661 ggtttctgga gtttaatgag ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa
      721 gtcgcctaag gtcactatca gctagcaaat atttcttgtc aaaaatgctc cactgacgtt
      781 ccataaattc ccctcggtat ccaattagag tctcatattc actctcaatc caaataatct
      841 gcaatggcaa ttaccttatc cgcaacttct ttacctattt ccgcccggat ctgatatcat
      901 cgatgaattc ccgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg
      961 cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa
     1021 aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat
     1081 tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac
     1141 tttattgcca aatgtttgaa cgatcgggga aattcgagct ctccggatcc cggtcggcat
     1201 ctactctatt cctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt
     1261 acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg
     1321 acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca
     1381 tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac
     1441 gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc
     1501 tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa
     1561 tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg
     1621 acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc
     1681 caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca
     1741 gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg
     1801 tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc
     1861 gcagcgatcg catccatggc ctccgcgacc ggctgcagtt atcatcatca tcatagacac
     1921 acgaaataaa gtaatcagat tatcagttaa agctatgtaa tatttacacc ataaccaatc
     1981 aattaaaaaa tagatcagtt taaagaaaga tcaaagctca aaaaaataaa aagagaaaag
     2041 ggtcctaacc aagaaaatga aggagaaaaa ctagaaattt acctgcagaa cagcgggcag
     2101 ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga tgcaataggt
     2161 caggctctcg ctgaattccc caatgtcaag cacttccgga atcgggagcg cggccgatgc
     2221 aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag
     2281 gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag
     2341 ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc
     2401 ggtgagttca ggctttttca tatcggggtc gtcctctcca aatgaaatga acttccttat
     2461 atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag
     2521 atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
     2581 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata
     2641 gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt
     2701 ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt
     2761 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag
     2821 tagacgagag tgtcgtgctc caccatgttg acggatctct aggacgcgtc gagtctagag
     2881 gagcatgcga cgtcgggccc tctagatgcg gccgcccgcc cagcaacgcc gcccctgtcc
     2941 ccgggcgccc gccgccgccg ggaagcaaac tagttcccgc cgccgcttcc cccaccgcac
     3001 agccgcccgc gaccaagaag acgcctcgcc acccccgcca catacgaccg acacgctcgt
     3061 cggacgccgt cacactcgtc ggacgcaggc agggcagcta gttggtctgt gggcgccgcg
     3121 tctcccctca ccggccgtct ccttcttcta cgcccgcaag ctgttcgaca gtttgccaag
     3181 gtacgaaaat gaactccgcc gatgagtgtt ttttccacaa tttcatttgc gactccgacg
     3241 attcgtcgtc cgacgacgag gaggagatat tggctgccat gttggtccat caccacctca
     3301 acagccagcg gccgttgttc cgtggctcca taccgggcca ccttcccgac gttgaaccgc
     3361 aatcgagaga gcgggcattt ccttcttcgg aaggactact ttgatacaac aaacccgttg
     3421 ttcaaacatc aaaaattccg tcgtcgtttc cgtatgagta ggcatctttt caaccgtatt
     3481 agagaggggg tggtcggcta tgatgactat tgcgagtgaa agaggatgcc gtcggcaaga
     3541 tcggtttctc ctcttatcag aaatgcactg ccgccatccg aatgcttgca tacggagtgc
     3601 acggtgatct cattgacgag tacgtccgta tgagtgagtc tacatgccta gagtccctgt
     3661 ataagttctg caaggctgct attgctgtgt ttggccctga gtacttgaga aagccgacaa
     3721 ctaaagatac atcttgtttg ttggcgacga atgccagaag gggctttcca gggatgcttg
     3781 gcagcataga ctgcatgcac tgggagtgga agaactgtcc ttctgcttgg caagggcaat
     3841 ataagggaca tgtcagggct tgcgctgtca tactaaggcc gtgacatctc aagatctctg
     3901 gatctggcac tctttctttg gcatggctgg atcacacaat gatatcaacg tgcttcagcg
     3961 ctcgcgggtg tttgctaggc ttgccgaagg caacagccca ccggcgaact ttactgtcaa
     4021 cggacacaac tacgacaaac gatactacct gggtgatagt atctatccta gtggaccact
     4081 attgtcaaga taatacccaa ccctgtcgga gagaagagga aaagatttgc ccatgagcaa
     4141 gagagtgcta ggaaggatgt cgagcgtgcc tttggtgttt tgcaatctcg atggggcatc
     4201 gttcggtatg ctgctaatat ctggagcacg cagaaactgg ggggaggtga tgactgcttg
     4261 tgtgatcatg cataatatga tcgtagaaga cgagcgaccg gaacgtctgt acgatcaagg
     4321 gtttcagttt cagagtgaga atgttgtgcc tgagcatgga gtagcagcaa cgtttgaaca
     4381 gttcatccaa tttcatcaag acatgcgtga ttgggaaact cacgtgcagc cgcaaaatga
     4441 tttggttgag tatatgtggg gtcatgttgg caaccaatag atatatcttc ttttattcgt
     4501 ttgcaaaact atgaaacatt tatttttatt tttatttgta tccggcttgt aaaactatac
     4561 tattttattt ggtaaactat gttatttgac aatatgtttg aatgcaaatc aatataatat
     4621 tgggcggcca gccagccacg ccgacacgta taggtcgacg cgttgggcgc gctgccgact
     4681 caaatctaaa aaagggcgga cgccgggcgg gcgggcgacc caaacgaaca aaaagcgaat
     4741 gaaatcgccg tccatttgga tcgctccatc ggagttgctc tgtctggccg ccaaatgggg
     4801 acgtgacgga caccgagtga actcgtgcgg tgtgtccgct tcacatctaa atttaggtaa
     4861 aaaaatgaat ccgtatggac atgttttgag aatgggtcgt tacgttgcga caatattttt
     4921 atccacaacg acctaaacaa atgcgggcag atgaaatgta tggctgcgtt ggagatgctg
     4981 ttaacggagc aaagtcgttg gtagtcggac cgagcaaagt aattgctgag gcaggtagtg
     5041 gacgagactg tagaaaggtt ccacgcttaa tatcttgagc agtacttgta gcacatgtgt
     5101 ccctgcaaag ctgtctccgt gctgtatacc atgtgaattg cttttctcaa aagtagtgga
     5161 gtaccctacc gtgtttcatt agtcttgccg gcttcataga caaaggattc ttttttcccg
     5221 tgccatcgcc atctgcaagc ccaaatcaac acacaccaga gagagagaga gagagagaga
     5281 gagagagcaa caatggcgga agttctggtg agcgcatcca caggggcaat gggttccctc
     5341 ctgcggaagt tgggagctat gttgacggat gaatacaagc tgctcaagaa cgtccgtggg
     5401 gacatcaagt tcctcaagga cgagctggag gtcatgtgcg cgttcctcct caagatgtca
     5461 gatgtggagg agcctgacga accaacaaag ctgcgtgtga cggcggtgcg ggagatgtcc
     5521 tataagatag aggacaacat cgacaagttc atggtcctcg tggaacagga acacgggtcc
     5581 tcctgttccg aggcggctca tggcgtcgcc aagctcatgg acaagtgcaa gaacttgctg
     5641 ccggacatca aggcccgccg caggatcgcc aaggaggtca aagacatcaa gaaagaaatt
     5701 aaggatgtca gcgacagatt ttcaaggtac cagatcttgt tctccttaca gtactattag
     5761 tacctaccat cagatttgtg accgatttgt tgccactcaa ggtacaagat tgacgactcc
     5821 tcctcctcta tgccggcaaa agacaaggtc gaccctcgac ttcgtgcagt ctacaaagac
     5881 gccgcagagc tcgtcggaat tgatggacca aaggatgaac ttgtcaagtg gctgaatgag
     5941 aaagaaggcc aatcactgaa atctgtctct attgttggat atggagggtt gggcaagacc
     6001 acgctcgcca accagatccg ggtcaacctt ggagcgacct tcgactgcgg ggcttttgtc
     6061 tcgatttcac gcaagcctga catgaaggcg atattaagat ctatattatc acaaatcacc
     6121 aagaaagacg atgcttgctc tagattagat gatatacaac tcatcatcga caagattcga
     6181 gaattcctcc aagacacaag gtatgtatgt gagatctgct acgaagtaac attgcttaat
     6241 tagctcatgc ataatcggtt gcctagaatt ttgctaatga agattgcttt gtgtgattta
     6301 ctttcgtcct caacttgtat gactgcgctt aaactttcaa gtcattgttt cttgcaactt
     6361 atgtcgatct gttcactaat taagatatag ctaaaatgtg tttgccgatt tctgtcgcca
     6421 cacattttct aatttgattg ccacaagttt gttaaacttg cggctatgtt ctgtttatta
     6481 aataattgtg acatggcgtc agtcaaaaca ataaagtaca tgcaattccc tctcttttgt
     6541 acaagataaa tatccgcacg cactacatga ctgcaaatat atctatcttc taaggaccca
     6601 aagcacttgc atgtgcagac cattgttacc ggatatgcag tacattaggt ttgtgattag
     6661 ccactttaag aacatagggt ttctagaggc tataaaccta cggttatcag tattataatc
     6721 ttgaatggat atcaaacatc cgttgaagaa tctatgccca taacacaaaa agagaaaagg
     6781 gaagaattaa tcatagtcag atcggaccag cctgactagc gtaacataat gacaacagta
     6841 ggttgttgcg tctttcctgg tttcgtagca atttgcatga cgaattgagg cgcagagcca
     6901 ccactcaata tgtggtccta ccaatcatga ggaaataact tgttaccaaa atgacaaaag
     6961 aaaataggta gtccccatca ttgtttcaat atctatcctc ttgcgaacat gctattggca
     7021 gtggcaaaac attgtacctt gccactggca acagctgcct ttactcggcc tgagcattca
     7081 tatttttttc ctcttggttg ttaaatcttg ctatttttcg cttgccttct atggcaatct
     7141 ttttttttct tatattgcca ccaaattgta tctaagagca actccaacac gccgacgcaa
     7201 tttttgtcca tttagatcgg ccaagcggac ggggtgtctg gttttgcctt tgggtggtgc
     7261 gcctacgcat tagtccgcgt gggcatttaa aaaacttaaa aagcttaaaa taattaaata
     7321 taaataaaaa tgcatattaa agcccgtcaa agtccacata attaaacata atacattaat
     7381 atattaataa aaaacagccg ccccagcccg cagccctagg cgtcttcgtc ggaggagtgg
     7441 gtgaggtcga agaatgccgg cagctcagcc cacgggaagg cctgctggtg ggcagccagc
     7501 accgcctgct ccgctgtctg cggcggcggc ggccctacct ggcaccgcgc acgcctcctc
     7561 ctcgcgctcc tgccgctcct cctcctcatc gcacgcccaa cgaagttttg gcgctcctcc
     7621 ccctcgggtt cccgctgcat gcggacctgg ccgtcgtacc gctcctcctc cagcgtgcgg
     7681 tccttccacg ccacgaggta ggccgcctcc tcctccatca cgcacagcca aatgggcggc
     7741 ttgctcatcc gcttgcagac gatgccctgc cattggtaaa cgccggtggg cgcgggtggt
     7801 gctagcgctg gtgcaggcgg tgacgatgtg gctggtggtc ccggcaggta gacactatag
     7861 ctggcggccg atagctgcag cgtgtcatcg aggccagacc actaggagtc ctcgcactaa
     7921 gactcctcgg tgacgtgatg ggccccctcc tccaatgcct tgaggaacgc cgcctcctcc
     7981 tcaacctact atttgaccac cggtggcggc ggtggagggc aggcttcgag ctggacggcg
     8041 tcgcggaggc gcacatggtg ctcgatggtg aaccagactt ctcagttagg ggagtctggc
     8101 ctcgatgggt ggagtcgcga cgctgctctg gcgtcaggag agcacgccgg gcccgaatct
     8161 cgttggcgcg cgcacgctcg gactgtggca ccgccggcac tggaatgtgg tgggggtcga
     8221 ggtgccagcc gtgggggaag ttcacgtccg gccacggcag tgagacgcgg tactcccggt
     8281 ggtactacgc gtggtgcacc gggatgtact ggtggtcccg tggcgctgcc gccctcccat
     8341 cccgcagcgc cgacaccaat gcgaacccct tcgctgccgg cctcaccggc ccttgcaacg
     8401 agccggcctc gtggtcgtgc ttgccttgcc cttacctttg ccaaagaaac ccatgacggc
     8461 tagggttttc tgtcaccggc gagggagcag atgtggacta gagtgggagg agaagtggat
     8521 gaggcccgcc ccgccgcacc cttgcaatta aaaagggcgt gatccggacg tttctagtca
     8581 ctgccatgcg ggcccgaggt aggtgggcgc cattgactac gtctgtggtg gtagttggct
     8641 ggcagacacg ccagcccgag gcgcacaccg agcggacgcg caacacgtcc gtctcgtgtt
     8701 cgtgtgaacg caaatcaggc acaaatttgg gctggaaatg cgtctacatg gatgccgaac
     8761 ggacgtgatt tgggtttgtg tccgcacgtt gggccggcgt ttttgtgcct ttcgacccta
     8821 atggacacac acggaccaaa tccgttggcg tgttagagtt gcttgtacct agtactacta
     8881 tgtgtggagt tgttatcttt gagtttctcc cccatctgta gctgtgtatt attgtgagat
     8941 gctctcagga tttcacttat tttagataat gacaactttc ttgatgctag acaattacat
     9001 caaggtgtat tatatctagg atgcacacag atgtgagatt gtttcaggat tttaggttac
     9061 ttggtaggtg ttactataaa tatctaatga gctctaatgt gtatgttcat gaaactgtgt
     9121 tgcaggtact ttatcataat tgatgatata tgggagttag gaacatggga aactttgaaa
     9181 tgtgcatttg tcaagaatac attgggcagc agaataatta tcacaacacg tatagttgat
     9241 gttgccaaat cttgttctcc ttctagtgaa gatcttgtct atgagatgaa accactcagt
     9301 gaggctgact cgaagaaatt gtttttcaag aggatatttg gctgtgaaga aagctgtcct
     9361 gatagcttga aagaagctgc caatgatatt ttgaaaaaat gccgtggcct acctctggcc
     9421 atcaatgcca tttctagcgt gttggtcaca acaagggaaa caaaagaaga gtgggatcgg
     9481 gtgcgacatt caatacgctc ttcaaaggtc aaaagtgata taatagagac catgaattac
     9541 atattgtctc ttagttattt tgatcttccc caccacctaa gaagctgctt attgtacctg
     9601 gctctgtttc ctgaagacca attgattgga aggaagcggt tagtacgcag atggatttct
     9661 gaaggcttta tccatggaga aagtggacaa gatctcatgg aattaggaga ggaatatttt
     9721 caccagcttg tgaacagaag tctaatacag cccggcaaca tagggtatga cggcaaggca
     9781 atgtattgcc gagtccatga caccatcctt gatttcctaa ttgacaaatc ctctgaagag
     9841 aacatgtgta ctgtcctaaa gaaacaatgc aagcctaatg gcatagttcg tcggctctct
     9901 ctaatgggga atgaggatga agaaatcgtg gagcaattgg atctgtcaca cgctcggtca
     9961 atcactgctt ttggggatat caagctattg ccttcgcttg ggaggtcaaa atgcttgcgc
    10021 gtgctagact tgcaagactg cgatcagttg gaaaatcatc atatcaagga tattgaaaga
    10081 ctctatcagc taagatactt ggatatctct tctacaggaa tcacggagct tcccaggcaa
    10141 attggggagt tgctgtatct agagacgcta gttgcctatg gattacgtga gcttcctgaa
    10201 agcacaagtc ggctacaacg actggcacgt ttgtttgttt actctggttg taagcttcca
    10261 ggtgggttgg gaaacttgat aaacttgcaa gagcttgact gcgttgatgc cttgcatttg
    10321 aagcatgtgg aagagctcgg caaactaaca aatctgagaa agctgagtat taaattggac
    10381 actggtggga ttgaaggcaa caaattagaa gagtccaagg aaaagctggt gtcctctctc
    10441 tgtaaactgg acgaatgcgg cctcctttcc ctgagtattg attattatct gagagaaaag
    10501 gatggagaag agccgttcct ccctgctttg ggttgtatcc aggaggtttt tgtatatgga
    10561 caagatatca gccggattag cagatggctc gcgtcacttc ccaacctaca caggttgctt
    10621 ttggacgatc cgaagataga gcagcaggat attgagatga ttggattgat acccaatctg
    10681 atcgatttga cactgccgcc tctatacaaa acagacgacg ctgggcggct catcatcagg
    10741 cgtgaaggat ttcaacagtt gcagaagttt gaggcttaca atactcgtat gggggtttta
    10801 atgtttgaac cgggggctat gccgaggctc aaggagctta aactccataa cttcatcgaa
    10861 aagcccaaat ctgctgcggt cgatttcgac tttggcatcc aacgcctctc cagccttgct
    10921 cgcctcactg tcagcttatc ctgcggtggc tggacggtgg cagaagtaga ggctgcggag
    10981 gatgctttca agagcatggc cgaggcgaac cccaaccgtc caatactgga gatgacaaga
    11041 tataatacgc agcatatgtt acaagacgag caaataggta tgacaggctc aggtactatt
    11101 aactagtttg tacttttgtc ttggtgagca gtagatcaaa agatgtgcat atgcttcaat
    11161 cttttttccg gtaatctatt tcagctacaa cgccggccgt caagtcctag tgcctaaacc
    11221 gagatgcgcc tttggttatg gagcccaagt cacgtactcg gattggatgc tgcagcaaat
    11281 gatacttctg tacatgtctg caagtatcac gaattagtgg aatgactagg aataagtaag
    11341 acaagtatca cgaattcaga gtcggatgca atttttttct tttcttttgg gcataagtag
    11401 attgtcattt gagtattttt ttccattaat atttgtgtat tcttagattg agactttgat
    11461 atgtaaccca gatttctgtt gttaggaaac acataccgtg tagcttatca gaagaaaaag
    11521 atctagcgtg catctatatt ctatcggact atgatatttg gcacatgtgt gccatataaa
    11581 caaaactgtc acacctctat ttccttcact ctaaatagat gtatacatta gtttgattct
    11641 ttcttctttc acgtgcattt ctccttctaa ttagatgcat acattagagg acaaaaaaga
    11701 cgatgtcatt ctagattttc tttattaaaa tatttaaaat gttttgtagc tcaaaccgtc
    11761 ggtccgatgt aaaaaccgtt tttacatata cgattcgtcg cgatgagatc tttgaaacta
    11821 gatcctatgt tgacatgttt cgatgacttt ttttaaccaa aatttaccat gccttttata
    11881 tgcaagttac cgtgctattt ccacataagt catcggaggc ataaaaatgg ttcaccgaca
    11941 ctacagttca tatattacca tgatgtttac atataagtta ccgggagggg gggggggggt
    12001 gtctgttttc aataatttcc ccaccgagtc aaatattacc atagtatatg tacgtaagtt
    12061 atcaggtatc cgatgggtat attaccatgg ctatctttta aaaaaatctc taatttaccg
    12121 cggtgtttgt atctaaatta tcaagtttgt ggtgcctaat ttactatgct atttacatac
    12181 aaaagttacc agtgtatttt ttgacaattt tttactattc ttccccaaat caagactatt
    12241 accgtgctgt ttacacacaa gttaccgggt atattttcaa caacttcatc cacccgaatc
    12301 aaacttatca tgatgtttgt gagtaactta tcatgtctgc aatgcgtata ttaccattct
    12361 atttaaacaa aatttaccaa cgatatgctt caaacaattt tttacgtggg tcaaagctag
    12421 catactgttt ttgcgtaagt tattgtatct gcaataaata tattaccgtg ctatttacct
    12481 agaagttatt gggggtaatg ttcttcaacg aaaaaaaatt cagggtcaaa aaattattgt
    12541 ggtgtttgaa cgagagttat taggtctccg atgggtaaat tatcatgtta tttgcacata
    12601 aattaccgag ggtatgcttt tcaacaactt cctccatcca ggtcaaagtt atcatggtgt
    12661 ttttgctcaa gttttcaagt ttgtggtgcg tatattacca cgatgttgac tgtttacaag
    12721 gtttttgagt cgccgactaa tcgccgacta atcgtcgagt cgtcgcggcg gcggccgcat
    12781 gcataagctt atcgatgata agctgtcaaa catgagaatt cagtacatta aaaacgtccg
    12841 caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata tcctgccacc
    12901 agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg atacaggcag
    12961 cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga ctttgctcat
    13021 gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac acggatgatc
    13081 tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg tgatcaaata
    13141 tcatctccct cgcagagatc cgaattatca gccttcttat tcatttctcg cttaaccgtg
    13201 acaggctgtc gatcttgaga actatgccga cataatagga aatcgctgga taaagccgct
    13261 gaggaagctg agtggcgcta tttctttaga agtgaacgtt gacgatcgtc gacggatctt
    13321 ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt
    13381 tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca
    13441 acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt
    13501 cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc
    13561 ggctaccgcc ggcgtaacag atgagggcaa gcggatggct gatgaaacca agccaaccag
    13621 gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg
    13681 cttctgtttc tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca
    13741 aaagcaccgc cggacatcag cgctatctct gctctcactg ccgtaaaaca tggcaactgc
    13801 agttcactta caccgcttct caacccggta cgcaccagaa aatcattgat atggccatga
    13861 atggcgttgg atgccgggca acagcccgca ttatgggcgt tggcctcaac acgattttac
    13921 gtcacttaaa aaactcaggc cgcagtcggt aacctcgcgc atacagccgg gcagtgacgt
    13981 catcgtctgc gcggaaatgg acgaacagtg gggctatgtc ggggctaaat cgcgccagcg
    14041 ctggctgttt tacgcgtatg acagtctccg gaagacggtt gttgcgcacg tattcggtga
    14101 acgcactatg gcgacgctgg ggcgtcttat gagcctgctg tcaccctttg acgtggtgat
    14161 atggatgacg gatggctggc cgctgtatga atcccgcctg aagggaaagc tgcacgtaat
    14221 cagcaagcga tatacgcagc gaattgagcg gcataacctg aatctgaggc agcacctggc
    14281 acggctggga cggaagtcgc tgtcgttctc aaaatcggtg gagctgcatg acaaagtcat
    14341 cgggcattat ctgaacataa aacactatca ataagttgga gtcattaccc aaccaggaag
    14401 ggcagcccac ctatcaaggt gtactgcctt ccagacgaac gaagagcgat tgaggaaaag
    14461 gcggcggcgg ccggcatgag cctgtcggcc tacctgctgg ccgtcggcca gggctacaaa
    14521 atcacgggcg tcgtggacta tgagcacgtc cgcgagctgg cccgcatcaa tggcgacctg
    14581 ggccgcctgg gcggcctgct gaaactctgg ctcaccgacg acccgcgcac ggcgcggttc
    14641 ggtgatgcca cgatcctcgc cctgctggcg aagatcgaag agaagcagga cgagcttggc
    14701 aaggtcatga tgggcgtggt ccgcccgagg gcagagccat gactttttta gccgctaaaa
    14761 cggccggggg gtgcgcgtga ttgccaagca cgtccccatg cgctccatca agaagagcga
    14821 cttcgcggag ctggtattcg tgcagggcaa gattcggaat accaagtacg agaaggacgg
    14881 ccagacggtc tacgggaccg acttcattgc cgataaggtg gattatctgg acaccaaggc
    14941 accaggcggg tcaaatcagg aataagggca cattgccccg gcgtgagtcg gggcaatccc
    15001 gcaaggaggg tgaatgaatc ggacgtttga ccggaaggca tacaggcaag aactgatcga
    15061 cgcggggttt tccgccgagg atgccgaaac catcgcaagc cgcaccgtca tgcgtgcgcc
    15121 ccgcgaaacc ttccagtccg tcggctcgat ggtccagcaa gctacggcca agatcgagcg
    15181 cgacagcgtg caactggctc cccctgccct gcccgcgcca tcggccgccg tggagcgttc
    15241 gcgtcgtctc gaacaggagg cggcaggttt ggcgaagtcg atgaccatcg acacgcgagg
    15301 aactatgacg accaagaagc gaaaaaccgc cggcgaggac ctggcaaaac aggtcagcga
    15361 ggccaagcag gccgcgttgc tgaaacacac gaagcagcag atcaaggaaa tgcagctttc
    15421 cttgttcgat attgcgccgt ggccggacac gatgcgagcg atgccaaacg acacggcccg
    15481 ctctgccctg ttcaccacgc gcaacaagaa aatcccgcgc gaggcgctgc aaaacaaggt
    15541 cattttccac gtcaacaagg acgtgaagat cacctacacc ggcgtcgagc tgcgggccga
    15601 cgatgacgaa ctggtgtggc agcaggtgtt ggagtacgcg aagcgcaccc ctatcggcga
    15661 gccgatcacc ttcacgttct acgagctttg ccaggacctg ggctggtcga tcaatggccg
    15721 gtattacacg aaggccgagg aatgcctgtc gcgcctacag gcgacggcga tgggcttcac
    15781 gtccgaccgc gttgggcacc tggaatcggt gtcgctgctg caccgcttcc gcgtcctgga
    15841 ccgtggcaag aaaacgtccc gttgccaggt cctgatcgac gaggaaatcg tcgtgctgtt
    15901 tgctggcgac cactacacga aattcatatg ggagaagtac cgcaagctgt cgccgacggc
    15961 ccgacggatg ttcgactatt tcagctcgca ccgggagccg tacccgctca agctggaaac
    16021 cttccgcctc atgtgcggat cggattccac ccgcgtgaag aagtggcgcg agcaggtcgg
    16081 cgaagcctgc gaagagttgc gaggcagcgg cctggtggaa cacgcctggg tcaatgatga
    16141 cctggtgcat tgcaaacgct agggccttgt ggggtcagtt ccggctgggg gttcagcagc
    16201 cagcgcttta ctggcatttc aggaacaagc gggcactgct cgacgcactt gcttcgctca
    16261 gtatcgctcg ggacgcacgg cgcgctctac gaactgccga taaacagagg attaaaattg
    16321 acaattgtga ttaaggctca gattcgacgg cttggagcgg ccgacgtgca ggatttccgc
    16381 gagatccgat tgtcggccct gaagaaagct ccagagatgt tcgggtccgt ttacgagcac
    16441 gaggagaaaa agcccatgga ggcgttcgct gaacggttgc gagatgccgt ggcattcggc
    16501 gcctacatcg acggcgagat cattgggctg tcggtcttca aacaggagga cggccccaag
    16561 gacgctcaca aggcgcatct gtccggcgtt ttcgtggagc ccgaacagcg aggccgaggg
    16621 gtcgccggta tgctgctgcg ggcgttgccg gcgggtttat tgctcgtgat gatcgtccga
    16681 cagattccaa cgggaatctg gtggatgcgc atcttcatcc tcggcgcact taatatttcg
    16741 ctattctgga gcttgttgtt tatttcggtc taccgcctgc cgggcggggt cgcggcgacg
    16801 gtaggcgctg tgcagccgct gatggtcgtg ttcatctctg ccgctctgct aggtagcccg
    16861 atacgattga tggcggtcct gggggctatt tgcggaactg cgggcgtggc gctgttggtg
    16921 ttgacaccaa acgcagcgct agatcctgtc ggcgtcgcag cgggcctggc gggggcggtt
    16981 tccatggcgt tcggaaccgt gctgacccgc aagtggcaac ctcccgtgcc tctgctcacc
    17041 tttaccgcct ggcaactggc ggccggagga cttctgctcg ttccagtagc tttagtgttt
    17101 gatccgccaa tcccgatgcc tacaggaacc aatgttctcg gcctggcgtg gctcggcctg
    17161 atcggagcgg gtttaaccta cttcctttgg ttccggggga tctcgcgact cgaacctaca
    17221 gttgtttcct tactgggctt tctcagccgg gatggcgcta agaagctatt gccgccgatc
    17281 ttcatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
    17341 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
    17401 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
    17461 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    17521 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    17581 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    17641 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    17701 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    17761 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    17821 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    17881 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    17941 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
    18001 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    18061 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatatcaa gaagatcctt
    18121 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    18181 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    18241 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    18301 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    18361 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    18421 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    18481 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaaa caagtggcag
    18541 caacggattc gcaaacctgt cacgcctttt gtgccaaaag ccgcgccagg tttgcgatcc
    18601 gctgtgccag gcgttaggcg tcatatgaag atttcggtga tccctgagca ggtggcggaa
    18661 acattggatg ctgagaacca tttcattgtt cgtgaagtgt tcgatgtgca cctatccgac
    18721 caaggctttg aactatctac cagaagtgtg agcccctacc ggaaggatta catctcggat
    18781 gatgactctg atgaagactc tgcttgctat ggcgcattca tcgaccaaga gcttgtcggg
    18841 aagattgaac tcaactcaac atggaacgat ctagcctcta tcgaacacat tgttgtgtcg
    18901 cacacgcacc gaggcaaagg agtcgcgcac agtctcatcg aatttgcgaa aaagtgggca
    18961 ctaagcagac agctccttgg catacgatta gagacacaaa cgaacaatgt acctgcctgc
    19021 aatttgtacg caaaatgtgg ctttactctc ggcggcattg acctgttcac gtataaaact
    19081 agacctcaag tctcgaacga aacagcgatg tactggtact ggttctcggg agcacaggat
    19141 gacgcctaac aattcattca agccgacacc gcttcgcggc gcggcttaat tcaggagtta
    19201 aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
    19261 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
    19321 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
    19381 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
    19441 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
    19501 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
    19561 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
    19621 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
    19681 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
    19741 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
    19801 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
    19861 cagtatcagc ccgtcatact tgaagctagg caggcttatc ttggacaaga agatcgcttg
    19921 gcctcgcgcg cagatcagtt ggaagaattt gttcactacg tgaaaggcga gatcaccaag
    19981 gtagtcggca aataatgtct aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt
    20041 aactcaagcg ttagagagct ggggaagact atgcgcgatc tgttgaaggt ggttctaagc
    20101 ctcgtacttg cgatggcatc ggggcaggca cttgctgacc tgccaattgt tttagtggat
    20161 gaagctcgtc ttccctatga ctactcccca tccaactacg acatttctcc aagcaactac
    20221 gacaactcca taagcaatta cgacaatagt ccatcaaatt acgacaactc tgagagcaac
    20281 tacgataata gttcatccaa ttacgacaat agtcgcaacg gaaatcgtag gcttatatat
    20341 agcgcaaatg ggtctcgcac tttcgccggc tactacgtca ttgccaacaa tgggacaacg
    20401 aacttctttt ccacatctgg caaaaggatg ttctacaccc caaaaggggg gcgcggcgtc
    20461 tatggcggca aagatgggag cttctgcggg gcattggtcg tcataaatgg ccaattttcg
    20521 cttgccctga cagataacgg cctgaagatc atgtatctaa gcaactagcc tgctctctaa
    20581 taaaatgtta ggagcttggc tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc
    20641 agcccctggg gggatgggag gcccgcgtta gcgggccggg agggttcgag aagggggggc
    20701 accccccttc ggcgtgcgcg gtcacgcgca cagggcgcag ccctggttaa aaacaaggtt
    20761 tataaatatt ggtttaaaag caggttaaaa gacaggttag cggtggccga aaaacgggcg
    20821 gaaacccttg caaatgctgg attttctgcc tgtggacagc ccctcaaatg tcaataggtg
    20881 cgcccctcat ctgtcagcac tctgcccctc aagtgtcaag gatcgcgccc ctcatctgtc
    20941 agtagtcgcg cccctcaagt gtcaataccg cagggcactt atccccaggc ttgtccacat
    21001 catctgtggg aaactcgcgt aaaatcaggc gttttcgccg atttgcgagg ctggccagct
    21061 ccacgtcgcc ggccgaaatc gagcctgccc ctcatctgtc aacgccgcgc cgggtgagtc
    21121 ggcccctcaa gtgtcaacgt ccgcccctca tctgtcagtg agggccaagt tttccgcgag
    21181 gtatccacaa cgccggcggc cggccgcggt gtctcgcaca cggcttcgac ggcgtttctg
    21241 gcgcgtttgc agggccatag acggccgcca gcccagcggc gagggcaacc agcccggtga
    21301 gcgtcggaaa ggg
//
