LOCUS       sequence-200234-       19356 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pVec8 containing Sr45 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19356)
  AUTHORS   Md Hatta MA, Ghosh S, Athiyannan N, Richardson T, Steuernagel B, Yu 
            G, Rouse MN, Ayliffe M, Lagudah ES, Radhakrishnan GV, Periyannan SK,
            Wulff BBH
  TITLE     Extensive Genetic Variation at the Sr22 Wheat Stem Rust Resistance 
            Gene Locus in the Grasses Revealed Through Evolutionary Genomics and
            Functional Analyses.
  JOURNAL   Mol Plant Microbe Interact. 2020 Nov;33(11):1286-1298. doi: 
            10.1094/MPMI-01-20-0018-R. Epub 2020 Oct 1.
  PUBMED    32779520
REFERENCE   2  (bases 1 to 19356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1094/MPMI-01-20-0018-R"; journalName: "Mol Plant Microbe 
            Interact"; date: "2020-11"; volume: "33"; issue: "11"; pages: 
            "1286-1298"
COMMENT     Sequence Label: pBW_0141
FEATURES             Location/Qualifiers
     source          1..19356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    480..504
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        628..807
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        660..843
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      complement(913..1165)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     intron          1893..2082
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     promoter        complement(2431..2775)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(2467..2487)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    10917..10941
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     oriT            11496..11605
                     /note="incP origin of transfer"
     mobile_element  11665..12432
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             13057..14205
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     primer_bind     complement(15394..15411)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15565..16153)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15645..15664)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             17250..18038
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      18713..19348
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcgacgatct tgctgcgttc ggatattttc gtggagttcc cgccacagac ccggattgaa
       61 ggcgagatcc agcaactcgc gccagatcat cctgtgacgg aactttggcg cgtgatgact
      121 ggccaggacg tcggccgaaa gagcgacaag cagatcacgc ttttcgacag cgtcggattt
      181 gcgatcgagg atttttcggc gctgcgctac gtccgcgacc gcgttgaggg atcaagccac
      241 agcagcccac tcgaccttct agccgaccca gacgagccaa gggatctttt tggaatgctg
      301 ctccgtcgtc aggctttccg acgtttgggt ggttgaacag aagtcattat cgcacggaat
      361 gccaagcact cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat
      421 aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg
      481 tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca
      541 atctgatcat gagcggagaa ttaagggagt cacgttatga cccccgccga tgacgcggga
      601 caagccgttt tacgtttgga actgacagaa ccgcaacgtt gaaggagcca ctcagccgcg
      661 ggtttctgga gtttaatgag ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa
      721 gtcgcctaag gtcactatca gctagcaaat atttcttgtc aaaaatgctc cactgacgtt
      781 ccataaattc ccctcggtat ccaattagag tctcatattc actctcaatc caaataatct
      841 gcaatggcaa ttaccttatc cgcaacttct ttacctattt ccgcccggat ctgatatcat
      901 cgatgaattc ccgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg
      961 cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa
     1021 aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat
     1081 tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac
     1141 tttattgcca aatgtttgaa cgatcgggga aattcgagct ctccggatcc cggtcggcat
     1201 ctactctatt cctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt
     1261 acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg
     1321 acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca
     1381 tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac
     1441 gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc
     1501 tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa
     1561 tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg
     1621 acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc
     1681 caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca
     1741 gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg
     1801 tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc
     1861 gcagcgatcg catccatggc ctccgcgacc ggctgcagtt atcatcatca tcatagacac
     1921 acgaaataaa gtaatcagat tatcagttaa agctatgtaa tatttacacc ataaccaatc
     1981 aattaaaaaa tagatcagtt taaagaaaga tcaaagctca aaaaaataaa aagagaaaag
     2041 ggtcctaacc aagaaaatga aggagaaaaa ctagaaattt acctgcagaa cagcgggcag
     2101 ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga tgcaataggt
     2161 caggctctcg ctgaattccc caatgtcaag cacttccgga atcgggagcg cggccgatgc
     2221 aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag
     2281 gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag
     2341 ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc
     2401 ggtgagttca ggctttttca tatcggggtc gtcctctcca aatgaaatga acttccttat
     2461 atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag
     2521 atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
     2581 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata
     2641 gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt
     2701 ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt
     2761 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag
     2821 tagacgagag tgtcgtgctc caccatgttg acggatctct aggacgcgtc gagtctagag
     2881 gagcatgcga cgtcgggccc tctagatgcg gccgcgaaga caatctgagc gttaggaggg
     2941 agctggcggc gtcgaactcc ttttcctaca tacacccttt tttgacggga catttacacc
     3001 gtttttttaa tgtatatgtg acagaaacat gttggtagaa tttgggcttc atttcgtttt
     3061 gcaatggccc ttctcaaaaa tatacttgta gctggaagca cccttttatt tgtatgtaac
     3121 ggaaacacat tggcagaact agtaaataat atttattttt gaactcgggt aatccccgta
     3181 atgcaaatat acacaagtct gaatagacgt ttaggaggtg ggaaagagaa agatcaagtt
     3241 caacttatcg ccaggaaacc gctgcgagtg cgcgcaatgg acactcacca tggggcgaaa
     3301 acccaatgac accaaactat actaacaatg ccatacaaag ctcatgcagt agcagcagcg
     3361 gccaaaggta ccaggagtct gaagcagcac ctaaacagcg acaaaccact acaaaacatg
     3421 cagagctgta tgagccagag caagtaaata ttttctgaag taaggaatcc aacaatgagt
     3481 cgcagttgtt tacgaattaa ggagctcggg tgagcccaga atttcagtca ttacaaacat
     3541 gatactacac cctcaggttt gtccggttca gataaaaaaa acatcaacat gacatgagcc
     3601 agccattcga tgtaacagac agcaacaacg atggctctca gccgaaacag agcaacttac
     3661 tacaacgctt tactaaaaaa ccaagcaaca tctctttatg caagcgactg ccatttaaaa
     3721 ggagcacatc acacaacctc tcggaacctt tttatccttc aattttttgc atgaccggtg
     3781 cccacccaac gtttatgaaa catctcagac gccactagca gtaacctgcg cccctggtac
     3841 ctgcacaacg caagtctttc ttctgtaagc tataccaaca gaacatccaa aatgcaactt
     3901 taccaactac acagcttagg tacaaatgga aactatttct gaagcaagca tcatttcctc
     3961 attagaacca ccctgaaatt catgaaccaa tcacacagtc tgtccatgct aacaggaact
     4021 atatcaaaac taaaagcaca cagcaatcat agagtgcccc caagtattac gagcaatcga
     4081 acaattaaga aggtatgatc cactgactct tttctactgc gaaaaggtaa attgtgccaa
     4141 gtgcattttt ctcagatact tatgccaaag aaacttctgt aaaattacct ttgcctgttc
     4201 agaatttccg tagctgctag agaagcacaa ttgaacaatg caatgacaag ctgagctcta
     4261 gagatggaaa aaaaggtgga gaataatatg atgagtaaat atgtatagat tgagagcaca
     4321 cctgaagcaa cgtcagaagc ctattcagac tcacgcttgc gcagtgaagg aatcatatat
     4381 ttccaggatt ttggttccca tagtttaggc cctgtacatg gaaaacaatg tcagaatatc
     4441 tctgtgaaca ttaatagcaa gtcaaacttt acaaataaat gatagtggga tagacataaa
     4501 agaatgatct gtgtacggtc actacacaag agagcatact tccatggtgt attaactttt
     4561 accaaaatga tagaaaatac agatcggcca cacatgacga cgtcaatcca agtcaaactt
     4621 acggtataca aataaatgat attggaatag ccatatcaga gaaactgtgt acgcggtcac
     4681 tacataccaa atgtgaaaat acaaggagtt ggataaactt gaaactacag gctgcaatat
     4741 gtacatctgt aaactataca gatggaaaaa gagatacaaa atggggtaaa tgaaattgaa
     4801 aggtaccttc attaggatgg cttgaaccag ccttagatag atccttcaat tcaaggctat
     4861 caagccgctg ttggagatgt ccatgaagtg gcttcatatt taaagctggg cagtattcaa
     4921 tgcggagctt ctgaagggcc gagtaactcc caagacttcc tggtacagat gccagatgtt
     4981 tgcagcccat aaggtataga tattccagtg acggtagatc tcccaaacaa tccagcgact
     5041 ccagcccatt gcaatcagaa atacacaaaa attggagtgc atctaactgc actggcacga
     5101 aacgaagctt cttacagcta actatacgca aataatcaag acatggcaaa ggattaattc
     5161 tgggtgaaag tgactgctct agcaaacatg tggacgtgag gtcattgaag tgttccgatg
     5221 atgatgctgt atcaaagtgt agtatactct ccaactctgt atcatcctgc cccagtatgg
     5281 actcaatact attgcaatcc agcatatcaa tacttgtgag agatggcggc agaacaaaaa
     5341 gctctgtcaa acttccacaa tgatgtattc ttaaattttt aagatttggc aggagttgat
     5401 cccttacttg agtacgggta cggcatcctt tcccctgggt gtgtcctact agcttgctgc
     5461 aggaattaat ggataaacta ttcaatgata ccaagcaacg gaactcttct tcaggccagt
     5521 agatgagcat atcacaagat tttatttcca aatgcacaag ctgaccaaac catttccaga
     5581 ccccaacagt tggctgcaat ggagttgagc agaacaaaaa gttgcaacca tccaacagca
     5641 ggttcgaaag agatacttca caatcctgat caagctctgt tcttggtgct gcttctgtat
     5701 cacggacgga caggccaacc ccagacaagc aagccatata cctggactta aatattgata
     5761 aggatagctg tgccttttct tcttttagag ctataaactt gagttttggt gcttcaggca
     5821 gaattgctag ctttgggcag tccttaattt taacattctc aagttgagga aatgctaact
     5881 cttctccttc tgttgccacc catctctcaa aaatctccaa tttttttaat ctgagaactt
     5941 ttaatgctgg aaatattgca gatgaactgt gattgcatag gctttgaagt ttgttcaacc
     6001 ttttcaaaac aagaacttca agggacttaa attgaccgaa ttggggaaat tcctcacaca
     6061 ttgtacaacc atctagctgg agctcggtca aatgctgcag aaaattaata cttaccaccc
     6121 atgcggggaa gccagtacct ctataggagc gtattcttag aaactccagg acagcatgag
     6181 gtttaagagc atcgagcaca ttcctgtggt ggcccaattc atcactgctg tcatcacccc
     6241 atttaagaga taaatgtgta agtttctctt tatttccaag gctgcatgct ttagcatcta
     6301 cttcagttgc aaactgcaga cgagatagct ctaactcacc accaagattc aagtcctgta
     6361 gttctctaat agtactgcaa ccaggactag agctcaccac aaaatatgtt agagtctgta
     6421 gagaagtgag ttgcccgagg cctggaggca tgcacttcaa tgatgagcat ccatttgtat
     6481 agaggtgacg gagattttcc atatacttca tatcctctgg aagttgatca agccttctgc
     6541 agttacaaag gttaagagtc tgtagattat acagtatgct tatttccttg ggaagctctt
     6601 cgatcaacat gttatttgag agatcaagat acctcaggtg ctgtaagtgc cttggccaga
     6661 gtggaagttt tctcaaactg aagagttgca gtgctcgcag ataattgcac ttcgacaagt
     6721 gtggtgctga gtcactggtg tagggatacg gatacagcag tgtctggaga gcgggagagt
     6781 gtttcttcag ataatcatcc aacagagttc cgatatactc atatgatgag aacaagtaac
     6841 gtgtaggacc agtggacagt aactcctttt cattaggcct atcaactata gtaagacaat
     6901 cttttcccat aacagacaag gcaatgtcat gcataagatc atgtatcttg catattgttc
     6961 taggacggag ctccccccac cactctcttc gagggattcg cttgacatct tgaaagaatg
     7021 acctccaagt taactcatca aaaatttctc tacctacctt ctcaagatta tcaccttctt
     7081 tcagtggtat gaaatcatgt gccatccata gctgaattaa tgtttcaaca ccaatctcat
     7141 aatctttggg aaatacagca caaaaagcaa agcattgttt caagtgtgat ggcaagtcat
     7201 catagctgag cttgagtaca agaaaagttt ttgtgccctc attgcaagtg ttgcttctgg
     7261 ctagtatgtc tgtccattct ttcatgctag tcttgttact caacatagac ccaaaggctt
     7321 tggcagctaa aggagagcct ccacatctgt ccataatctt atcaaccaca tcgcttagct
     7381 cagcagcctt tggcttttgc aaacagaatg ctctattctc aaatatttcc ttcagaaata
     7441 ctttatctag atttctgaga ttatggctat cagcaataca catcttcata attcgtgcta
     7501 cttgcacatt gcgagtggtc gtcaatattg cactcccctt cccaccgtgc ttcaggcagg
     7561 ttttcagttt ttcccactta tcagcatctt cattccatac atcatccaac acaatgaggt
     7621 atctcttccc acttacttct ttctgaaggt tctgcaatgc cttttcacga tggatctcat
     7681 tagtctcaca gatgttgttt gcaatcttca caacatcaaa atcatctgac acacaacacc
     7741 acctctggag cttgaaatgc tccttgatta tagggtcatt gtagacaagc tgagcaaagg
     7801 tggtcttgcc cagcccgccc attccaacaa tgggaaggac gatgaggtcc ctatcgccag
     7861 cttgatcaat caatatatca acgatcttct tcttctcttc gtttctggac ctgctgacaa
     7921 tatccttctc agagtcaatt attatggaat ctgtgttgcg ccactgcttg gatggtggtg
     7981 cttgctgagt ttggttgaat ccgaagtcat tcatctctct gacaaggccc tcaattttgc
     8041 gcacaatcct gcacaacttc ttgcccatcc tgtaacgaaa tacaatgggg ttgtgagcag
     8101 ggaagaggct tacgatgtca aagccaagct tcttgtagtg tcccttcttc ttggcgtcac
     8161 gccggagggc ctcgtacttg aactcatcaa agacatcgtt cgcctcatag gccaccttct
     8221 tgagtgcttc gagccaagcg cgcactccag gtagggaagc tcctttcttc tccgcatctt
     8281 ggatgatgtc caagatggct ggcagctggc gcttgagggt ttcgcgctgc tccttcatgc
     8341 catccatcac cttgtactgg tccagcaggt agctggatgc cgtgttcatc aacgtggata
     8401 ccagtggcct gactacaaac tccgccatca ttgagagctc tcttgatcaa tcagctgaaa
     8461 cctgcaaatc gatgaattta gggatctgta acacaatcca agctctggtg gaactgagtt
     8521 ggcaagccaa atcgatgaat gtggggatct ggaccaagcc caccttttga tgcttcagag
     8581 gagagcagaa cacaacggcg gcgtcttcgt cggatggtca cgctgtgcgg acaacgaagc
     8641 gggtcagtgg attaaacgct ttgctcgatg gggacaacac ggcggccctg taataaaaac
     8701 ttatagccgg gtcagcgcct tggatgcatg gtgctctgaa taatgactgg gcaaatcaat
     8761 gagactcgtc aagcaggaga agcaatgact ttttagaccc aagcattgct actttactgg
     8821 aaacaaatcg acaagaaaca tacccggagg ttggatgaga ttagcaacaa ggtggatgga
     8881 tctgttcaag gcagcacaat ggaagcgggg acagaggaag tccaacagca gtcaagagag
     8941 taaattgctt ggaatacagt ggtactccgt actagataga tacagagccc cacgacccca
     9001 ccctggattc aatcaacaca aatcggtcag aatctcaagc aggagaagta ataactttta
     9061 gatccctttt tagatccaat aactggtaaa aaaacagagc agaggaagac cccactgcca
     9121 cggccacggg tgtcaaggtc ggatcgccgt cagtggagaa agagcagagg aagaccccac
     9181 ctttttgtgc ttcacggcag agcagaacac agcggcggtg gcctcgtcgg acaacgaagc
     9241 gaaagcggcg gctgggcacc agaggccaga tccgtgaggg gacaagacgg cgcttcactc
     9301 aatctggaag tataatctgg gaaatcagca gatgaacaag gaaggtgggg atgtggaaga
     9361 tttcagtagc aagaaagatg agacaaagtt accaggtcag tggattccat gcgtggtgct
     9421 ctgaataatg attggaaact ccatgattct tgctagctgc tccactgcca ccccaaatga
     9481 ttcaaccaac acaaatcgat cagactccca agcaagcagt gaagcaggag acgtaagttt
     9541 ttagatccaa gcgtacttca gctggaaaga aatatacctc cactccacag gtgtcagggg
     9601 cgagctcgga ggttggacga gctcaccaaa caagaagccg gatcgaccag gagatgagga
     9661 ctgagatgat aggaagggag cactctgtcg gatccgtgag cgtgtgggca ccagaggcca
     9721 gatccgtgag cgtgtggagg tcagtggatt cgatgcatgg tgcaaaatag atgaatatgg
     9781 ggagctgtaa tccaatcaaa actgtgtgaa gctttaattt caacgagcta tagaacagag
     9841 ttgccaatcc aaactgtgat atccatccaa acccaccttt gatacttcac ggcagaggag
     9901 aacacagccg cggcgtcaac accggatggc cgtcaccgtt gatgcttcac cggcagagga
     9961 ggacagagca gaacacagcg gcgtcgtagg ggacaagacg gcggcggatg tcgcttcact
    10021 cgatctggaa gaataagtaa gaacttacca tcaagcaaat cagcagatta ataagaagag
    10081 aatagcaaga aagatgggag ggaaattttt accaggccac tagtatggtg ctctgaatag
    10141 tcactggaaa ctgccacccg aaatctccac ggaggatgga tgagatcagc aacaaggcgg
    10201 atcgatctgt tccaggcagc gcaatggagg agactgagag gaagcggaga cgaggagcag
    10261 aggaagacca acagcactca agagagtaaa ttgcttggaa tatccatcct agatagatac
    10321 agagccccac gatcccaccc tgagttcaat caacacaaat ggatcagaat ctgaagcagg
    10381 agaagtaata agttttagat ccaagcattg ctactttaag tggaaacaaa tcgacaggaa
    10441 atatatatac ccaaaaagtc cacggtcacc aatagagcag tcagttggca ggggcgaggc
    10501 caataggagg cgagatgaga tcagcaacaa gggcggatct atcttattcg agggaggagg
    10561 agaggactga gatgagaatg aggagaggaa gggagtactc agtggaggga gacgaggagc
    10621 atcaagagag gatactactt tagcacgctc ctcgaatact agtacgtatt acagggaggg
    10681 ataccctctg cctttgggct gatatgttta ttcagtgggt acctatggtc agtccaccag
    10741 ccatgcttca ccaaatatta tccctaaata gcccttttga aatttaagta agcacatcct
    10801 caacactctt aaagcggccg catgcataag cttatcgatg ataagctgtc aaacatgaga
    10861 attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
    10921 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca
    10981 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt
    11041 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
    11101 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
    11161 gcgttgctgc ctgtgatcaa atatcatctc cctcgcagag atccgaatta tcagccttct
    11221 tattcatttc tcgcttaacc gtgacaggct gtcgatcttg agaactatgc cgacataata
    11281 ggaaatcgct ggataaagcc gctgaggaag ctgagtggcg ctatttcttt agaagtgaac
    11341 gttgacgatc gtcgacggat cttttccgct gcataaccct gcttcggggt cattatagcg
    11401 attttttcgg tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt
    11461 gtagactttc cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca
    11521 cccgcgagcg ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg
    11581 aatcctgctc tgcgaggctg gccggctacc gccggcgtaa cagatgaggg caagcggatg
    11641 gctgatgaaa ccaagccaac caggggtgat gctgccaact tactgattta gtgtatgatg
    11701 gtgtttttga ggtgctccag tggcttctgt ttctatcagc tgtccctcct gttcagctac
    11761 tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat cagcgctatc tctgctctca
    11821 ctgccgtaaa acatggcaac tgcagttcac ttacaccgct tctcaacccg gtacgcacca
    11881 gaaaatcatt gatatggcca tgaatggcgt tggatgccgg gcaacagccc gcattatggg
    11941 cgttggcctc aacacgattt tacgtcactt aaaaaactca ggccgcagtc ggtaacctcg
    12001 cgcatacagc cgggcagtga cgtcatcgtc tgcgcggaaa tggacgaaca gtggggctat
    12061 gtcggggcta aatcgcgcca gcgctggctg ttttacgcgt atgacagtct ccggaagacg
    12121 gttgttgcgc acgtattcgg tgaacgcact atggcgacgc tggggcgtct tatgagcctg
    12181 ctgtcaccct ttgacgtggt gatatggatg acggatggct ggccgctgta tgaatcccgc
    12241 ctgaagggaa agctgcacgt aatcagcaag cgatatacgc agcgaattga gcggcataac
    12301 ctgaatctga ggcagcacct ggcacggctg ggacggaagt cgctgtcgtt ctcaaaatcg
    12361 gtggagctgc atgacaaagt catcgggcat tatctgaaca taaaacacta tcaataagtt
    12421 ggagtcatta cccaaccagg aagggcagcc cacctatcaa ggtgtactgc cttccagacg
    12481 aacgaagagc gattgaggaa aaggcggcgg cggccggcat gagcctgtcg gcctacctgc
    12541 tggccgtcgg ccagggctac aaaatcacgg gcgtcgtgga ctatgagcac gtccgcgagc
    12601 tggcccgcat caatggcgac ctgggccgcc tgggcggcct gctgaaactc tggctcaccg
    12661 acgacccgcg cacggcgcgg ttcggtgatg ccacgatcct cgccctgctg gcgaagatcg
    12721 aagagaagca ggacgagctt ggcaaggtca tgatgggcgt ggtccgcccg agggcagagc
    12781 catgactttt ttagccgcta aaacggccgg ggggtgcgcg tgattgccaa gcacgtcccc
    12841 atgcgctcca tcaagaagag cgacttcgcg gagctggtat tcgtgcaggg caagattcgg
    12901 aataccaagt acgagaagga cggccagacg gtctacggga ccgacttcat tgccgataag
    12961 gtggattatc tggacaccaa ggcaccaggc gggtcaaatc aggaataagg gcacattgcc
    13021 ccggcgtgag tcggggcaat cccgcaagga gggtgaatga atcggacgtt tgaccggaag
    13081 gcatacaggc aagaactgat cgacgcgggg ttttccgccg aggatgccga aaccatcgca
    13141 agccgcaccg tcatgcgtgc gccccgcgaa accttccagt ccgtcggctc gatggtccag
    13201 caagctacgg ccaagatcga gcgcgacagc gtgcaactgg ctccccctgc cctgcccgcg
    13261 ccatcggccg ccgtggagcg ttcgcgtcgt ctcgaacagg aggcggcagg tttggcgaag
    13321 tcgatgacca tcgacacgcg aggaactatg acgaccaaga agcgaaaaac cgccggcgag
    13381 gacctggcaa aacaggtcag cgaggccaag caggccgcgt tgctgaaaca cacgaagcag
    13441 cagatcaagg aaatgcagct ttccttgttc gatattgcgc cgtggccgga cacgatgcga
    13501 gcgatgccaa acgacacggc ccgctctgcc ctgttcacca cgcgcaacaa gaaaatcccg
    13561 cgcgaggcgc tgcaaaacaa ggtcattttc cacgtcaaca aggacgtgaa gatcacctac
    13621 accggcgtcg agctgcgggc cgacgatgac gaactggtgt ggcagcaggt gttggagtac
    13681 gcgaagcgca cccctatcgg cgagccgatc accttcacgt tctacgagct ttgccaggac
    13741 ctgggctggt cgatcaatgg ccggtattac acgaaggccg aggaatgcct gtcgcgccta
    13801 caggcgacgg cgatgggctt cacgtccgac cgcgttgggc acctggaatc ggtgtcgctg
    13861 ctgcaccgct tccgcgtcct ggaccgtggc aagaaaacgt cccgttgcca ggtcctgatc
    13921 gacgaggaaa tcgtcgtgct gtttgctggc gaccactaca cgaaattcat atgggagaag
    13981 taccgcaagc tgtcgccgac ggcccgacgg atgttcgact atttcagctc gcaccgggag
    14041 ccgtacccgc tcaagctgga aaccttccgc ctcatgtgcg gatcggattc cacccgcgtg
    14101 aagaagtggc gcgagcaggt cggcgaagcc tgcgaagagt tgcgaggcag cggcctggtg
    14161 gaacacgcct gggtcaatga tgacctggtg cattgcaaac gctagggcct tgtggggtca
    14221 gttccggctg ggggttcagc agccagcgct ttactggcat ttcaggaaca agcgggcact
    14281 gctcgacgca cttgcttcgc tcagtatcgc tcgggacgca cggcgcgctc tacgaactgc
    14341 cgataaacag aggattaaaa ttgacaattg tgattaaggc tcagattcga cggcttggag
    14401 cggccgacgt gcaggatttc cgcgagatcc gattgtcggc cctgaagaaa gctccagaga
    14461 tgttcgggtc cgtttacgag cacgaggaga aaaagcccat ggaggcgttc gctgaacggt
    14521 tgcgagatgc cgtggcattc ggcgcctaca tcgacggcga gatcattggg ctgtcggtct
    14581 tcaaacagga ggacggcccc aaggacgctc acaaggcgca tctgtccggc gttttcgtgg
    14641 agcccgaaca gcgaggccga ggggtcgccg gtatgctgct gcgggcgttg ccggcgggtt
    14701 tattgctcgt gatgatcgtc cgacagattc caacgggaat ctggtggatg cgcatcttca
    14761 tcctcggcgc acttaatatt tcgctattct ggagcttgtt gtttatttcg gtctaccgcc
    14821 tgccgggcgg ggtcgcggcg acggtaggcg ctgtgcagcc gctgatggtc gtgttcatct
    14881 ctgccgctct gctaggtagc ccgatacgat tgatggcggt cctgggggct atttgcggaa
    14941 ctgcgggcgt ggcgctgttg gtgttgacac caaacgcagc gctagatcct gtcggcgtcg
    15001 cagcgggcct ggcgggggcg gtttccatgg cgttcggaac cgtgctgacc cgcaagtggc
    15061 aacctcccgt gcctctgctc acctttaccg cctggcaact ggcggccgga ggacttctgc
    15121 tcgttccagt agctttagtg tttgatccgc caatcccgat gcctacagga accaatgttc
    15181 tcggcctggc gtggctcggc ctgatcggag cgggtttaac ctacttcctt tggttccggg
    15241 ggatctcgcg actcgaacct acagttgttt ccttactggg ctttctcagc cgggatggcg
    15301 ctaagaagct attgccgccg atcttcatat gcggtgtgaa ataccgcaca gatgcgtaag
    15361 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    15421 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    15481 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    15541 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    15601 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    15661 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    15721 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    15781 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    15841 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    15901 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    15961 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
    16021 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    16081 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    16141 aaaaggatat caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    16201 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    16261 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    16321 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    16381 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    16441 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    16501 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    16561 ccagtctatt aaacaagtgg cagcaacgga ttcgcaaacc tgtcacgcct tttgtgccaa
    16621 aagccgcgcc aggtttgcga tccgctgtgc caggcgttag gcgtcatatg aagatttcgg
    16681 tgatccctga gcaggtggcg gaaacattgg atgctgagaa ccatttcatt gttcgtgaag
    16741 tgttcgatgt gcacctatcc gaccaaggct ttgaactatc taccagaagt gtgagcccct
    16801 accggaagga ttacatctcg gatgatgact ctgatgaaga ctctgcttgc tatggcgcat
    16861 tcatcgacca agagcttgtc gggaagattg aactcaactc aacatggaac gatctagcct
    16921 ctatcgaaca cattgttgtg tcgcacacgc accgaggcaa aggagtcgcg cacagtctca
    16981 tcgaatttgc gaaaaagtgg gcactaagca gacagctcct tggcatacga ttagagacac
    17041 aaacgaacaa tgtacctgcc tgcaatttgt acgcaaaatg tggctttact ctcggcggca
    17101 ttgacctgtt cacgtataaa actagacctc aagtctcgaa cgaaacagcg atgtactggt
    17161 actggttctc gggagcacag gatgacgcct aacaattcat tcaagccgac accgcttcgc
    17221 ggcgcggctt aattcaggag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga
    17281 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
    17341 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
    17401 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
    17461 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
    17521 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
    17581 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
    17641 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
    17701 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
    17761 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
    17821 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
    17881 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct aggcaggctt
    17941 atcttggaca agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgttcact
    18001 acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc
    18061 gacgccgctt cgcggcgcgg cttaactcaa gcgttagaga gctggggaag actatgcgcg
    18121 atctgttgaa ggtggttcta agcctcgtac ttgcgatggc atcggggcag gcacttgctg
    18181 acctgccaat tgttttagtg gatgaagctc gtcttcccta tgactactcc ccatccaact
    18241 acgacatttc tccaagcaac tacgacaact ccataagcaa ttacgacaat agtccatcaa
    18301 attacgacaa ctctgagagc aactacgata atagttcatc caattacgac aatagtcgca
    18361 acggaaatcg taggcttata tatagcgcaa atgggtctcg cactttcgcc ggctactacg
    18421 tcattgccaa caatgggaca acgaacttct tttccacatc tggcaaaagg atgttctaca
    18481 ccccaaaagg ggggcgcggc gtctatggcg gcaaagatgg gagcttctgc ggggcattgg
    18541 tcgtcataaa tggccaattt tcgcttgccc tgacagataa cggcctgaag atcatgtatc
    18601 taagcaacta gcctgctctc taataaaatg ttaggagctt ggctgccatt tttggggtga
    18661 ggccgttcgc ggccgagggg cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc
    18721 gggagggttc gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg
    18781 cagccctggt taaaaacaag gtttataaat attggtttaa aagcaggtta aaagacaggt
    18841 tagcggtggc cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct gcctgtggac
    18901 agcccctcaa atgtcaatag gtgcgcccct catctgtcag cactctgccc ctcaagtgtc
    18961 aaggatcgcg cccctcatct gtcagtagtc gcgcccctca agtgtcaata ccgcagggca
    19021 cttatcccca ggcttgtcca catcatctgt gggaaactcg cgtaaaatca ggcgttttcg
    19081 ccgatttgcg aggctggcca gctccacgtc gccggccgaa atcgagcctg cccctcatct
    19141 gtcaacgccg cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca
    19201 gtgagggcca agttttccgc gaggtatcca caacgccggc ggccggccgc ggtgtctcgc
    19261 acacggcttc gacggcgttt ctggcgcgtt tgcagggcca tagacggccg ccagcccagc
    19321 ggcgagggca accagcccgg tgagcgtcgg aaaggg
//
